Dry vs. wet testing: quasi-static tensile experiment setup for polymer based biomaterials

2021 ◽  
Vol 7 (2) ◽  
pp. 637-639
Author(s):  
Daniela Arbeiter ◽  
Stefan Oschatz ◽  
Kerstin Lebahn ◽  
Sabine Illner ◽  
Niels Grabow
Keyword(s):  
Biofilm Formation ◽  
Polymer Materials ◽  
Fine Tuning ◽  
Test Medium ◽  
Nonwoven Materials ◽  
Different Temperatures ◽  
Static Tensile ◽  
Matrix Property ◽  
Tensile Experiment ◽  
Property Modification

Abstract Polymer materials can be manufactured with high reproducibility and do offer the potential for chemical modification. This enables matrix property modification and fine-tuning of several material characteristics, such as tissue-implant interaction, inflammatory potential or susceptibility to biofilm formation. Whereas manufacturing protocols are crucial for the resulting material properties, also the evaluation in terms of performance and safety has to be considered. Regarding this, both, temperature and composition of test medium may affect the physicochemical properties of implant materials. The present study addresses the influence of test medium compared to dry test conditions, each at two different temperatures, on the mechanical properties of elastomeric film and nonwoven materials.

scholarly journals Chemical sanitizers to control biofilms formed by two Pseudomonas species on stainless steel surface

2012 ◽  
Vol 32 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Danila Soares Caixeta ◽  
Thiago Henrique Scarpa ◽  
Danilo Florisvaldo Brugnera ◽  
Dieyckson Osvani Freire ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Skim Milk ◽  
304 Stainless Steel ◽  
Stainless Steel Surface ◽  
Aisi 304 ◽  
Pseudomonas Species ◽  
Sodium Dichloroisocyanurate ◽  
Different Temperatures

The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of the adhered P. aeruginosa cells at 7 and 28 °C and those on the biofilm, respectively. The hydrogen peroxide was more effective in the reduction of adhered cells of P. fluorescens at 7 °C.

Effect of lactic acid stress on biofilm formation of Escherichia coli O26 at different temperatures

2020 ◽  
Author(s):  
Xiaoyu Mi ◽  
Jie Hu ◽  
Su Zhang ◽  
Siqi Wang ◽  
Wangchen Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Biofilm Formation ◽  
Acid Stress ◽  
Different Temperatures

Biofilm Formation and Biocides Sensitivity of Pseudomonas marginalis Isolated from a Maple Sap Collection System

2006 ◽  
Vol 69 (10) ◽  
pp. 2411-2416 ◽  
Author(s):  
L. LAGACÉ ◽  
M. JACQUES ◽  
A. A. MAFU ◽  
D. ROY
Keyword(s):  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Biofilm Formation ◽  
Planktonic Cells ◽  
Collection System ◽  
Biofilm Growth ◽  
Biofilm Production ◽  
Different Temperatures ◽  
Maple Sap ◽  
Scanning Electron

The susceptibility of planktonic and biofilm cells of Pseudomonas marginalis toward four commonly used biocides at different temperatures (15 and 30°C) and biofilm growth times (24 and 48 h) was assessed. Using the MBEC biofilm device, biofilm production in maple sap was shown to be highly reproducible for each set of conditions tested. Biofilm formation was influenced by growth temperature and time. A temperature of 15°C and incubation time of 24 h yielded fewer CFU per peg and showed fewer adhered cells and typical biofilm structures, based on scanning electron microscopy observations as compared with other conditions. Minimal biofilm eradication concentration values for P. marginalis were significantly greater (P < 0.001) than were MBCs for planktonic cells and for every biocide tested, with the exception of minimal biofilm eradication concentration values for peracetic acid at 15°C and 24 h. Sodium hypochlorite and peracetic acid sanitizers were able to eliminate P. marginalis biofilms at lower concentrations as compared with hydrogen peroxide– and quaternary ammonium– based sanitizers (P < 0.001). According to the results obtained, sodium hypochlorite and peracetic acid sanitizers would be more appropriate for maple sap collection system sanitation.

Transcriptional analysis of genes related to biofilm formation, stress-response, and virulence in Listeria monocytogenes strains grown at different temperatures

2014 ◽  
Vol 64 (4) ◽  
pp. 1707-1714 ◽  
Author(s):  
Luiza Pieta ◽  
Flavia Brusch Garcia ◽  
Gustavo Pelicioli Riboldi ◽  
Luisa Abruzzi de Oliveira ◽  
Ana Paula Guedes Frazzon ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Response ◽  
Biofilm Formation ◽  
Transcriptional Analysis ◽  
Different Temperatures

scholarly journals Experimental and semianalytical investigation of X850 ± IM190 CFRP bolted joints

2020 ◽  
Vol 29 ◽  
pp. 096369351989500
Author(s):  
Boling He
Keyword(s):  
Bolted Joints ◽  
Design Parameters ◽  
Material System ◽  
Commercial Aircraft ◽  
Reinforced Polymer ◽  
Epoxy Material ◽  
Static Tensile ◽  
Curing Method ◽  
Tensile Experiment ◽  
Bolt Diameter

Considering the fact that the foundation data for a new X850 ± IM190 carbon/epoxy material system adopted in commercial aircraft industry are extremely scarce in the literature, an in-plane, static tensile experiment was carried out to investigate the bearing performance of double-lap, single-bolt joints in X850 ± IM190 carbon fiber-reinforced polymer (CFRP) composites. The effects of ply ratio, 0° layers’ combination percentage, bolt diameter, and curing method were considered. Then, special attention was paid to determine the design parameters of X850 ± IM190 CFRP bolted joints, such as tensile strength of un-notched laminate and stress concentration relief factor. Based on these design parameters, an efficient semianalytical approach was established to obtain the ultimate bearing strength of the joints. The failure prediction exhibited excellent agreement with the experimental data. These results will play an important role in design and strength evaluation of X850 ± IM190 CFRP bolted joints.

scholarly journals The Toxicological Testing and Thermal Decomposition of Drive and Transport Belts Made of Thermoplastic Multilayer Polymer Materials

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2232 ◽  
Author(s):  
Piotr Krawiec ◽  
Łukasz Warguła ◽  
Daniel Małozięć ◽  
Piotr Kaczmarzyk ◽  
Anna Dziechciarz ◽  
...  
Keyword(s):  
Thermal Decomposition ◽  
Degradation Kinetics ◽  
Combustion Products ◽  
Polymer Materials ◽  
Toxic Substances ◽  
Butadiene Rubber ◽  
Different Temperatures ◽  
Increasing Temperature ◽  
In Fire ◽  
Kinetics Of

The article presents the potential impact of flat drive and transport belts on people’s safety during a fire. The analysis distinguished belts made of classically used fabric–rubber composite materials reinforced with cord and currently used multilayer polymer composites. Moreover, the products’ multilayers during the thermal decomposition and combustion can be a source of emissions for unpredictable and toxic substances with different concentrations and compositions. In the evaluation of the compared belts, a testing methodology was used to determine the toxicometric indicators (WLC50SM) on the basis of which it was possible to determine the toxicity of thermal decomposition and combustion products in agreement with the standards in force in several countries of the EU and Russia. The analysis was carried out on the basis of the registration of emissions of chemical compounds during the thermal decomposition and combustion of polymer materials at three different temperatures. Moreover, the degradation kinetics of the polymeric belts by using the thermogravimetric (TGA) technique was evaluated. Test results have shown that products of thermal decomposition resulting from the neoprene (NE22), leder leder (LL2), thermoplastic connection (TC), and extra high top cower (XH) belts can be characterized as moderately toxic or toxic. Their toxicity significantly increases with the increasing temperature of thermal decomposition or combustion, especially above 450 °C. The results showed that the belts made of several layers of polyamide can be considered the least toxic in fire conditions. The TGA results showed that NBR/PA/PA/NBR belt made with two layers of polyamide and the acrylonitrile–butadiene rubber has the highest thermal stability in comparison to other belts.

scholarly journals Effect of Batch and Fed-Batch Growth Modes on Biofilm Formation by Listeria monocytogenes at Different Temperatures

2009 ◽  
Vol 59 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Diana A. Rodrigues ◽  
Marta A. Almeida ◽  
Pilar A. Teixeira ◽  
Rosário T. Oliveira ◽  
Joana C. Azeredo
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Fed Batch ◽  
Growth Modes ◽  
Different Temperatures ◽  
Batch Growth

Biofilm Formation and Morphotypes of Salmonella enterica subsp. arizonae Differs from Those of Other Salmonella enterica Subspecies in Isolates from Poultry Houses

2016 ◽  
Vol 79 (7) ◽  
pp. 1127-1134 ◽  
Author(s):  
A. LAMAS ◽  
I. C. FERNANDEZ-NO ◽  
J. M. MIRANDA ◽  
B. VÁZQUEZ ◽  
A. CEPEDA ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Public Health Problem ◽  
Nutrient Concentrations ◽  
Cellulose Production ◽  
Biofilm Production ◽  
Poultry Houses ◽  
High Prevalence ◽  
Different Temperatures ◽  
Strain Dependent

ABSTRACT Salmonella serovars are responsible for foodborne diseases around the world. The ability to form biofilms allows microorganisms to survive in the environment. In this study, 73 Salmonella strains, belonging to four different subspecies, were isolated from poultry houses and foodstuffs and tested. Biofilm formation was measured at four different temperatures and two nutrient concentrations. Morphotypes and cellulose production were evaluated at three different temperatures. The presence of several genes related to biofilm production was also examined. All strains and subspecies of Salmonella had the ability to form biofilms, and 46.57% of strains produced biofilms under all conditions tested. Biofilm formation was strain dependent and varied according to the conditions. This is the first study to analyze biofilm formation in a wide number of Salmonella enterica subsp. arizonae strains, and no direct relationship between the high prevalence of Salmonella enterica subsp. arizonae strains and their ability to form biofilm was established. Morphotypes and cellulose production varied as the temperature changed, with 20°C being the optimum temperature for expression of the red, dry, and rough morphotype and cellulose. Salmonella enterica subsp. arizonae, whose morphotype is poorly studied, only showed a smooth and white morphotype and lacked the csgD and gcpA genes that are implicated in biofilm production. Thus, Salmonella biofilm formation under different environmental conditions is a public health problem because it can survive and advance through the food chain to reach the consumer.

scholarly journals Yellow electrochromic polymer materials with fine tuning electrofluorescences by adjusting steric hindrance of side chains

RSC Advances ◽  
2017 ◽  
Vol 7 (41) ◽  
pp. 25444-25449 ◽  
Author(s):  
Jian Liu ◽  
Yuchen Shi ◽  
Jingchuan Wu ◽  
Mei Li ◽  
Jianming Zheng ◽  
...  
Keyword(s):  
Steric Hindrance ◽  
External Potential ◽  
Polymer Materials ◽  
Fine Tuning ◽  
Side Chains ◽  
Structure Property ◽  
Structure Property Relationships ◽  
Electrochromic Polymer

A series of novel conducting conjugated yellow-to-transmissive electrochromic (EC) polymers were designed to research their structure–property relationships, achieving electrofluorescent (EF) switching with applied external potential.

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