Efficiency of selection–biotechnological system of selection for creation of breeding source material of sunflower resistant to herbicides and broomrape

Helia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Viktoria Babych ◽  
Mykola Kuchuk ◽  
Yaroslava Sharipina ◽  
Miroslav Parii ◽  
Yaroslav Parii ◽  
...  
Keyword(s):  
High Resistance ◽  
Normal Development ◽  
Source Material ◽  
Immature Embryos ◽  
Hybrid Combination ◽  
Selection For ◽  
The Creation ◽  
Efficiency Of Selection ◽  
Tribenuron Methyl

Abstract The sunflower is a strategically important oil crop. Every year the area under this crop grows, and the rapid returning of sunflowers back to the fields provokes the formation of new more aggressive races of broomrape (Orobanche cumana Wallr.). Broomrape is a parasite that interferes with the normal development of sunflower and can lead to significant crop losses. For creating a sunflower hybrid (F1) it is needed to cross the parental components, which have a complex of important traits, among which there is a resistance to the herbicides and a broomrape. Considering that the creation of each of the components of the hybrid involves many years of painstaking work in the breeding process, modern approaches and methods are used to accelerate the creation of a new source material. Thus, using the technology of cultivating immature embryos in vitro culture, it is possible to reduce the time to create lines resistant to herbicides, for example. And during selection for resistance to pathogenic organisms, testing is most often used against an artificial infectious background, both in the field and in laboratory conditions, in order to differentiate the material on this basis. The aim of this work was to establish the effectiveness system when creating an initial breeding material resistant to herbicides and broomrape. As a result of testing the lines on an artificial infectious background, was identified plants which have high resistance to the G-race broomrape and were isolated from hybrid combinations resistant to tribenuron-methyl and imidazolinones. Thus, among the analyzed plants which are resistant to tribenuron‐methyl, four lines were isolated, which are highly resistant to the G-race broomrape from a hybrid combinations BH0118/SURES–2 (101/1, 101/4, 101/6, 101/7), and BH0318/SURES–2 (101/21, 101/24, 101/28, 101/30), and five lines (101/11, 101/12, 101/16, 101/17, 101/18) from a hybrid combination BH0218/SURES–2. Among imidazolinone-resistant sunflower lines – line 3 was isolated as highly resistant to the G-race broomrape.

scholarly journals Use of sunflower immature embryos culture in in vitro for fast creation of fertility restorer resistant to tribenuron methyl herbicide

2020 ◽  
Vol 27 ◽  
pp. 23-28
Author(s):  
V. O. Babych ◽  
O. I. Varchenko ◽  
M. V. Kuchuk ◽  
M. F. Parii ◽  
Ya. F. Parii ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Herbicide Resistance ◽  
Immature Embryos ◽  
Culture Methods ◽  
Fertility Restorer ◽  
Homozygous Genotype ◽  
Tribenuron Methyl

Aim. Acceleration of the sunflower lines homozygosity for isolation of the fertility restorer resistant to tribenuron methyl herbicide using in vitro culture of sunflower immature embryos. Methods. Methods of immature sunflower embryos cultivation in in vitro culture; methods of classical crossing. Results. As a result, homozygous genotype was created and isolated from three combinations (BH0118xSURES-2, BH0218xSURES-2 and BH0318xSURES-2) for 10 parent sunflower lines. Conclusions. The use of the technology of sunflower immature embryos cultivation in in vitro culture effectively accelerates the sunflower lines homozygosity for the fertility restorer isolation resistant to tribenuron methyl herbicide. The involvement of immature sunflower embryos cultivation in in vitro culture methods can reduce time for creation of initial parental sunflower lines twice. Keywords: sunflower, immature embryos, in vitro culture, herbicide resistance, tribenuron methyl.

scholarly journals GENETIC SOURCES AND NEW GENPLASMA - THE BASIS OF INTRODUCED MAIZE MATERIAL

2020 ◽  
pp. 130-142
Author(s):  
Akhmad Al Sheikh Kaddur ◽  
Vitalii Zhemoida ◽  
Oleksandr Makarchuk ◽  
Volodymyr Alokhin
Keyword(s):  
Genetic Diversity ◽  
Crop Production ◽  
Test Methods ◽  
Source Material ◽  
Initial Growth ◽  
Original Material ◽  
Joint Research ◽  
Late Emergence ◽  
The Creation ◽  
Efficiency Of Selection

The efficiency of selection work depends on the availability of the source maize material with genetic diversity, high combination ability and a set of economically valuable traits. Modern maize hybrids are created using only a small portion of gene plasma. Only 5-8% of the species maize diversity is used in the modern breeding process. Of great importance for successful breeding research and effective selection is the introduced material, especially from different parts of the globe. Each form, line, variety or hybrid is characterized by a set of many features and properties. The most important of them change more under the influence of adaptation, when grown in new conditions. Scientists of the Department of genetics, breeding and seed production named after prof. M.O. Zelensky NULES of Ukraine have been working together with the Department of Crop Production of the University of Aleppo (Syrian Arab Republic) for more than 20 years to expand the gene pool, developed a scheme, methods of approaches to the introduced forms for their analysis, testing, selection, followed by the creation of both source material and the creation of high-yielding hybrids in Ukraine and Syria. The article presents materials that highlight the importance of different approaches to the selection of source material, genetic sources, as well as the importance of the new gene plasma in the creation of self-pollinating lines, its preservation and subsequent use in breeding programs. The results of many years of research and reports of their results provide an opportunity to summarize the indicators of the migration effect from one area to another. It is established that the use maize samples of Syrian origin begins with overcoming the low cold resistance and slowed down the initial growth of maize. That is why the original forms of Syrian selection when grown in the conditions of "VP ADS NULES of Ukraine" (Pshenichne village, Vasylkiv district, Kyiv region) have a long growing season and late emergence of panicles. This shortcoming can be eliminated only by selection. The authors have developed proposals for a joint research program, test methods, recommended scheme of work with the original material of Syrian and Ukrainian maize selection. Key words: maize, source material, introduction, cell and genetic engineering, genetic diversity, genetic resources, sources of traits, collections, adaptation.

scholarly journals Reproductive Technologies and Genomic Selection in Cattle

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Patrice Humblot ◽  
Daniel Le Bourhis ◽  
Sebastien Fritz ◽  
Jean Jacques Colleau ◽  
Cyril Gonzalez ◽  
...  
Keyword(s):  
Genomic Selection ◽  
Genetic Selection ◽  
Reproductive Traits ◽  
Reproductive Technologies ◽  
Combined Use ◽  
Reproductive Techniques ◽  
Selection For ◽  
Efficiency Of Selection ◽  
Near Future

The recent development of genomic selection induces dramatic changes in the way genetic selection schemes are to be conducted. This review describes the new context and corresponding needs for genomic based selection schemes and how reproductive technologies can be used to meet those needs. Information brought by reproductive physiology will provide new markers and new improved phenotypes that will increase the efficiency of selection schemes for reproductive traits. In this context, the value of the reproductive techniques including assisted embryo based reproductive technologies (Multiple Ovaluation Embryo Transfer and Ovum pick up associated toin vitroFertilization) is also revisited. The interest of embryo typing is discussed. The recent results obtained with this emerging technology which are compatible with the use of the last generation of chips for genotype analysis may lead to very promising applications for the breeding industry. The combined use of several embryo based reproductive technologies will probably be more important in the near future to satisfy the needs of genomic selection for increasing the number of candidates and to preserve at the same time genetic variability.

Improvement of effectiveness in maize breeding

2006 ◽  
Vol 54 (3) ◽  
pp. 351-358 ◽  
Author(s):  
P. Pepó
Keyword(s):  
Recurrent Selection ◽  
Genetic Manipulation ◽  
Field Testing ◽  
Tissue Cultures ◽  
Maize Breeding ◽  
Breeding Programmes ◽  
Speed Up ◽  
Selection For

Plant regeneration via tissue culture is becoming increasingly more common in monocots such as maize (Zea mays L.). Pollen (gametophytic) selection for resistance to aflatoxin in maize can greatly facilitate recurrent selection and the screening of germplasm for resistance at much less cost and in a shorter time than field testing. In vivo and in vitro techniques have been integrated in maize breeding programmes to obtain desirable agronomic attributes, enhance the genes responsible for them and speed up the breeding process. The efficiency of anther and tissue cultures in maize and wheat has reached the stage where they can be used in breeding programmes to some extent and many new cultivars produced by genetic manipulation have now reached the market.

Устойчивые сорта корнеплодов в муссонном климате юга Дальнего Востока России

2018 ◽  
Author(s):  
Y.G. Mikheev ◽  
I.A. Vanyushkina ◽  
V.I. Leunov
Keyword(s):  
High Resistance ◽  
Geographical Origin ◽  
Russian Far East ◽  
Far East ◽  
Source Material ◽  
The South ◽  
Root Crops ◽  
The Russian Far East ◽  
Resistance To Diseases

Представлены результаты изучения исходного материала столовой моркови и свеклы разного эколого-географического происхождения по устойчивости к патогенной флоре. Для условий Дальнего Востока созданы высокопродуктивные, устойчивые к патогенам сорта и гибриды столовых корнеплодов.The article presents the results of the study of the source material of table carrots and beets of different ecological and geographical origin for resistance to diseases. Geniuses for breeding of high-productive variety samples of root crops with rather high resistance to diseases in the conditions of the South of the Russian Far East are revealed.

Reading Genesis Through Chronicles: The Creation of the Sons of Jacob

2016 ◽  
Vol 7 (2) ◽  
pp. 138-168
Author(s):  
Andrew Tobolowsky
Keyword(s):  
Historical Memory ◽  
Source Material ◽  
Biblical Studies ◽  
Dynamic Nature ◽  
Dynamic Power ◽  
The Creation ◽  
Book Of Genesis ◽  
Over Time

Scholars are increasingly aware of the dynamic nature of the interaction between the nine-chapter-long genealogy that begins the book of Chronicles and its source material. However, little attention has been paid to the role this interaction might have played in the creation of some key biblical ideas, particularly in the “eponymous imagination” of the tribes as literally the sons of Jacob. Through comparison with scholarly approaches to the pseudo-Hesiodic Catalogue of Women and an investigation into the ramifications for biblical studies of ethnic theory and historical memory on the fluidity of ethnicity and memory over time, this article seeks to reassess the dynamic power of the Chronicles genealogy as an ethnic charter for the elites of Persian Yehud. Focus on the distinctive imagination of Israel in the crucial narratives in the book of Genesis, as compared with narratives elsewhere in the primary history, and the contributions of the Chronicles genealogy to their redefinition, allows us to address the Bible’s dependence upon the lens the Chronicles genealogy imposes upon it.

Divergent Selection for In Vitro Dry Matter Digestibility in Smooth Bromegrass 1

Crop Science ◽  
1986 ◽  
Vol 26 (6) ◽  
pp. 1123-1126 ◽  
Author(s):  
N. J. Ehlke ◽  
M. D. Casler ◽  
P. N. Drolsom ◽  
J. S. Shenk
Keyword(s):  
Dry Matter ◽  
Divergent Selection ◽  
Smooth Bromegrass ◽  
Dry Matter Digestibility ◽  
Selection For

The Neuropathic Diathesis, or the Diathesis of the Degenerate

1888 ◽  
Vol 34 (146) ◽  
pp. 167-176
Author(s):  
G. T. Revington
Keyword(s):  
Family History ◽  
Normal Development ◽  
Personal History ◽  
Striking Feature ◽  
General Paralysis ◽  
History Of ◽  
The Creation ◽  
The Individual ◽  
The Mind

I think that the foregoing statistics, and those which follow, together with the large number of cases which I quote, and which connect general paralysis with almost every form of neurotic manifestation, will prove conclusively that neurotic inheritance is a striking feature in the causation of general paralysis. I question whether a distinction between “the cerebral and the insane element” in general paralysis can be maintained. If general paralysis is not a degeneration of the mind-tissue, then the pathology of insanity has no existence, and I would say that the subtle influence for evil, which is transmitted from parents, whose brains are deteriorated by neurotic outbursts, or soaked in alcohol, or wrecked by physiological immorality, tends strongly towards such degeneration. If insanity is, as Dr. Savage says, a perversion of the ego, then a general paralytic is the in-sanest of the insane. We know that the children of a melancholic parent, for example, may develop any form of neurosis—in other words, it is not that melancholia or general paralysis, or any other definite disease, is transmitted, but that a certain tendency to deviate from normal development is transmitted. This tendency to deviate is the neurotic diathesis, and the form of its development is determined by collateral circumstances, and a certain series of collateral circumstances determine the development of general paralysis. Perhaps neurotic inheritance may mean in some cases a limited capital of nervous energy, and if this is wasted recklessly the individual breaks down suddenly and pathologically, as we all do slowly and physiologically. I would also point out that considering the number of histories of insanity which owing to ignorance or reticence we, do not receive, and considering that we never receive information as to the existence of the slighter neuroses, it is marvellous that we get so high a percentage as 51. Of the 145 general paralytics with a reliable history, 38 had a family history of insanity, 28 a family history of drink, 8 of both, 43 had a personal history of drink, 8 of a previous attack too remote to be considered, at least, according to our present ideas, as part of the disease, and the vast majority had a history of some physiological irregularity which must be considered as conducive to the creation of an acquired neurosis. We may now pass to some further statistics.

scholarly journals Enhancing Terpene Yield from Sugars via Novel Routes to 1-Deoxy-d-Xylulose 5-Phosphate

2014 ◽  
Vol 81 (1) ◽  
pp. 130-138 ◽  
Author(s):  
James Kirby ◽  
Minobu Nishimoto ◽  
Ruthie W. N. Chow ◽  
Edward E. K. Baidoo ◽  
George Wang ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Xylose Reductase ◽  
Pentose Phosphate ◽  
Terpene Biosynthesis ◽  
Content Type ◽  
E Coli ◽  
Pathway Expression ◽  
Terpene Synthesis ◽  
Selection For

ABSTRACTTerpene synthesis in the majority of bacterial species, together with plant plastids, takes place via the 1-deoxy-d-xylulose 5-phosphate (DXP) pathway. The first step of this pathway involves the condensation of pyruvate and glyceraldehyde 3-phosphate by DXP synthase (Dxs), with one-sixth of the carbon lost as CO2. A hypothetical novel route from a pentose phosphate to DXP (nDXP) could enable a more direct pathway from C5sugars to terpenes and also circumvent regulatory mechanisms that control Dxs, but there is no enzyme known that can convert a sugar into its 1-deoxy equivalent. Employing a selection for complementation of adxsdeletion inEscherichia coligrown on xylose as the sole carbon source, we uncovered two candidate nDXP genes. Complementation was achieved either via overexpression of the wild-typeE. coliyajOgene, annotated as a putative xylose reductase, or via various mutations in the nativeribBgene.In vitroanalysis performed with purified YajO and mutant RibB proteins revealed that DXP was synthesized in both cases from ribulose 5-phosphate (Ru5P). We demonstrate the utility of these genes for microbial terpene biosynthesis by engineering the DXP pathway inE. colifor production of the sesquiterpene bisabolene, a candidate biodiesel. To further improve flux into the pathway from Ru5P, nDXP enzymes were expressed as fusions to DXP reductase (Dxr), the second enzyme in the DXP pathway. Expression of a Dxr-RibB(G108S) fusion improved bisabolene titers more than 4-fold and alleviated accumulation of intracellular DXP.

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