Determination of thermal, molecular changes, and functional properties in stabilized rice bran

2021 ◽  
Vol 17 (4) ◽  
pp. 247-256
Author(s):  
Raciye Meral
Keyword(s):  
Thermal Properties ◽  
Rice Bran ◽  
Lipase Activity ◽  
Radical Scavenging ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Rice Bran Lipase ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

Abstract In this study, stabilization was performed using three different oven temperatures (110, (T1) 130 (T2) and 150 °C(T3)) in the oven set at 10% moisture. The effect of stabilization on lipase activity, functional, and thermal properties of rice bran. Lipase activity of control (27.92 U/g) was to be higher than stabilized rice bran samples. Starch gelatinization peak temperature ranged from 70.05 (T3) to 85.09 °C (control). DPPH scavenging effect of control was 12.99 mmol TE. The highest DPPH radical scavenging effect value (15.72 mmol TE) was determined in the T3 sample. ABTS radical scavenging effect of stabilized samples significantly increased (p < 0.05) and the increase for T1, T2, and T3 was found to be 73, 75, and 77%, respectively. The highest ferulic (4284 μg/g) and p-coumaric acid (1180 μg/g) contents were determined in T3 and T1 samples, respectively. The content of bound ferulic and p-coumaric acids of stabilized rice bran samples was 2.41 and 2.33 times higher than control samples. While prolamine content was 3.38–4.18 mg/mL; glutelin content ranged from 4.45 to 5.11 mg/mL. An obvious state change during stabilization was observed in all samples around 70–85.9 °C. The results of the study revealed that stabilization might change the functional and thermal properties of rice bran.

scholarly journals Antibacterial, Antioxidant and Cytotoxic Properties of Crinum asiaticum Bulb Extract

2012 ◽  
Vol 28 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Md Atiar Rahman ◽  
Rumana Sharmin ◽  
Md Nazim Uddin ◽  
Md Sohel Rana ◽  
Nazim Uddin Ahmed
Keyword(s):  
Radical Scavenging ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Scavenging Effect ◽  
Ic50 Value ◽  
Radical Scavenging Effect ◽  
Dpph Scavenging ◽  
Crinum Asiaticum

Antibacterial effect of Crinum asiaticum bulb extract (1mg/disc) was tested on four Gram- positive and six Gram-negative bacteria by disc diffusion method using kanamycin (30 ìg/disc) as standard antibiotic disc. The bulb extract (250-1000mg/disc) showed significant zone of inhibition against all Gram-positive and Gram-negative bacteria ranging from 12-14 mm in diameter. Antioxidant potential of the same extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The extract showed remarkable free radical scavenging effect (95.96%) providing the IC50 value of 5.62 for the bulb extract and 5.46 for ascorbic acid (standard antioxidant) at the concentration of 1000 ìg/ml. The bulb extract was found to be (LC50 value 94.06 ?g/ml) in Brine-Shrimp lethality test. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11801 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 1-5

DPPH radical-scavenging effect on some constituents from the aerial parts of Lippia triphylla

2007 ◽  
Vol 62 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Masateru Ono ◽  
Eriko Oda ◽  
Takemi Tanaka ◽  
Yoshihiko Iida ◽  
Toru Yamasaki ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Aerial Parts ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging ◽  
Lippia Triphylla

scholarly journals Some bioactive component analysis of herbal capsules

2021 ◽  
Vol 17 (2) ◽  
pp. 057-062
Author(s):  
Mustafa Yaşar ◽  
Sibel Bayıl Oğuzkan ◽  
Halil İbrahim Uğraş
Keyword(s):  
Radical Scavenging ◽  
Protective Activity ◽  
Scavenging Effect ◽  
Bioactive Component ◽  
Scavenging Capacity ◽  
Dpph Method ◽  
Pbr322 Plasmid ◽  
Total Antioxidant ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging

Since the beginning of the evaluation of plants in terms of human health, the bioactivity properties of plants have been studied in the laboratory and thus standards have been brought to the treatment methods with plants. For this purpose, some bioactive component analyzes of 18 herbal capsules and 2 liquid herbal syrups produced by Naturin Company were performed in our current study. In this context, the total oxidant and antioxidant status of these plant mixtures were evaluated. The free radical capacities of the samples were determined by 1, 1-diphenyl-2-picrylhydrazil (DPPH) method and their DNA protective activities were determined using pBR322 plasmid DNA. Total antioxidant level (TAL) and Total oxidant level (TOL) activities were performed using Rel Assay diagnostic kit. All 3 of the stinging nettle samples showed DNA protective activity. The clean sample containing milk thistle extract also showed DNA Protective activity. In both the syrup samples (My guard and DTX-19) results were detected in the direction of positive protection on DNA. When the DPPH radical scavenging capacity was examined, it was determined that the best results were in juniper, nettle and thistle thistle samples, and the samples with syrup had a very good radical scavenging effect, and all of the samples showed antioxidant activity. This study is a preliminary and supportive study in order to elucidate the properties that can be used as drug active ingredients in traditional medicine, especially in the field of pharmacology.

Preparation, Purification and Identification of Antioxidant Peptides with Bienzyme Hydrolysis from Rice Bran Protein

2012 ◽  
Vol 610-613 ◽  
pp. 72-80 ◽  
Author(s):  
De Jun Mei ◽  
Guo Ping Yu ◽  
An Min Sun
Keyword(s):  
Rice Bran ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Antioxidant Peptides ◽  
Rice Bran Protein ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

The rice bran protein (RBP) was then hydrolyzed with various proteases (papain, flavorzyme, neutrase, protamex, and trypsin) to prepare antioxidant peptides. The rice bran protein hydrolysates (RBPH) were assessed using method of DPPH radical scavenging ability. Hydrolysate prepared with papain and flavorzyme (activity ratio 1:1) was found to have the highest antioxidant activity (IC50=6.778±0.21 mg/ml). This hydrolysate was purified using ultrafiltration, RBPH-III (Mw＜3KDa) had the highest DPPH and hydroxyl radical scavenging activity (IC50 value of 6.56±0.28, 5.43±0.22, respectively) and highest reducing power activity (1.02±0.18 at 4 mg/mL). Later, RBPH-III was fractionated by SP-SephadexC-25 cation-exchange column into six fractions (A–F), fraction F with the highest DPPH scavenging activity, was then separated by size exclusion chromatography on a SephadexG-25 into three major fractions (F1–F3). Fraction F2 exhibited the highest DPPH scavenging activity was choose to fractionate by reversed-phase high performance liquid chromatography (RP-HPLC), seven antioxidant peptides were isolated, The F2-5 peptide displayed the highest DPPH radical-scavenging activity (58.2±1.63%; at 250 μg/ml) among these peptides, the amino acids composition of F2-5 was determined, which might play an important role on its antioxidant activity. In addition, purified peptide did show remarkable inhibition rate on SGC-7901 cells proliferation, and it also revealed the dose-dependent relationship. The results of this study suggest that rice bran protein hydrolysates are good source of natural antioxidants.

DPPH Radical-scavenging Effect of Several Phenylpropanoid Compounds and Their Glycoside Derivatives

2005 ◽  
Vol 125 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Hitoshi TOMINAGA ◽  
Yuka KOBAYASHI ◽  
Takashi GOTO ◽  
Kazuo KASEMURA ◽  
Masato NOMURA
Keyword(s):  
Radical Scavenging ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging

scholarly journals Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach

Antioxidants ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 137 ◽  
Author(s):  
Qamar Ahmed ◽  
Alhassan Alhassan ◽  
Alfi Khatib ◽  
Syed Shah ◽  
Muhammad Hasan ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bioactive Constituents ◽  
Butanol Fraction ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging

The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p < 0.05), as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p < 0.05). Afzelechin 3-O-alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.

scholarly journals Antioxidant capacity of blackcurrant (Ribes nigrum L.) leaves and buds

2021 ◽  
Vol 14 (1) ◽  
pp. 117-129
Author(s):  
Cs. Orbán ◽  
É. Kis ◽  
Cs. Albert ◽  
É. Molnos
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Measurement Systems ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Pattern Of Variation

Abstract The antioxidant capacity is the combined free radical scavenging effect of all antioxidant compounds found in the studied system. There is a growing need for accurate, numerical determination of this capacity (for easier comparison), so there are many analytical procedures, methods, and measurement systems available to researchers. Neither one is able to model the totality of real, naturally occurring reactions; therefore, conclusions about the antioxidant power of the studied sample can be drawn only after using several methods. In this work, the total phenolic content (TPC) of blackcurrant leaves and buds was determined, and the antioxidant capacity was tested using the DPPH and FRAP assays. 80% methanol was the most effective in the extraction of phenolics followed by 80% ethanol, while for the antioxidant capacity the acetone (50%)/water/acetic acid (2%) mixture proved to be the best. Significant differences were observed between cultivars and sampling dates, but the pattern of variation during the harvest period was similar for all cultivars.

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