scholarly journals Modulators of KATP channels in the prevention of oxidative stress and antioxidant capacity improvement in the rat heart with different resistance to hypoxia upon cobalt treatment

2016 ◽  
Vol 60 (2) ◽  
pp. 195-206 ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Katp Channels ◽  
Heart Tissue ◽  
Individual Resistance ◽  
Individual Resistance To Hypoxia ◽  
Resistance To Hypoxia ◽  
Channel Opener ◽  
Antioxidant Defence System ◽  
Oxidatively Modified ◽  
The Individual

AbstractIntroduction:The main goal of the study was to investigate the effect of KATPchannel modulators on development of oxidative stress in the heart of rats showing different resistance to hypoxia.Material and Methods:The study has been performed on rats showing high- (HR) or low-resistance (LR) to hypoxia under modulators of ATP-sensitive potassium (KATP) channel opener pinacidil (0.06 mg/kg) and blocker glibenclamide (1 mg/kg) upon cobalt (Co) treatment (30 mg of cobalt chloride/kg b.w., 3 h). Changes in the oxidative stress parameters of the heart tissue, such as lipid peroxidation (LPO), level of oxidatively modified protein (OMP), and antioxidant defence system (superoxide dismutase - SOD, catalase -CAT, glutathione peroxidase - GPx, glutathione reductase - GR) as well as total antioxidant activity (TAA) were analysed.Results:Co treatment caused a significant decrease in SOD and CAT activity in the heart of LR rats and GPx activity in HR rats. It also led to a decrease in OMP level in the heart of rats with HR in comparison with controls.Conclusion:The obtained results suggest that individual resistance to hypoxia plays a crucial role in Co actions and provides evidence that the effects of KATPchannel opener pinacidil in the heart are mediated through different pathways of the antioxidative system, depending on the individual resistance to hypoxia. Pinacidil exerts a protective effect on the heart tissue by preventing the LPO decrease and significantly reducing OMP levels, as well as increasing TTA in rats with LR.

scholarly journals Аналіз ефективності інсуфляції кисню, субстанції “кд-234” і реамберину на процеси вільнорадикального окиснення при гострому респіраторному дистрес-синдромі в щурів

2017 ◽  
Author(s):  
M. I. Marushchak ◽  
S. O. Savchuk ◽  
U. P. Gevko ◽  
R. S. Usinsky
Keyword(s):  
Oxidative Stress ◽  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Distress Syndrome ◽  
Control Level ◽  
Male Rats ◽  
Individual Resistance To Hypoxia ◽  
Resistance To Hypoxia ◽  
Liver Tissues

Introduction. In previous studies, we have demonstrated the development of oxidative stress in case of experimental acute respiratory distress syndrome (ARDS), which was characterized by a statistically significant increasing of active product soft hiobarbituric acid, dienic and triene conjugates concentration in liver tissues, decreasing of superoxide dismutase and catalase activity, and the content of SH-groups in animals with different resistance to hypoxia.The aim of the study – to compare the effectiveness of the combination of oxygen insufflation, the substance "KD-234" and reamberin on oxidative stress level in rats with associated chest and both hip injuries.Research Methods. Experimental studies were performed on 92 white nonlinear adult male rats aged 6–8 months. The animals were selected according to their individual resistance to hypoxia. Resistance of rats to acute hypoxia was carried out according to the method of V. Ya. Berezovskyi. Depending on the resistance to hypoxia, all animals were divided into medium-resistant (MRH) and low-resistant (LRH). We used G. Mutute-Bello experimental model. In the second test group for the correction we used the substance "KD-234", which was diluted in distilled water for injections and injected intragastric through a probe at a dose of 50 mg / kg, in the third study group – reamberin, at a dose of 10 ml / kg was intraperitoneally administered to animals one hour before the ARDS simulation.Results and Discussion. The combination of oxygen insufflation with "KD-234" or reamberin had a significant positive effect on lipid peroxidation compared to oxygen insufflation, but the results were higher from the control level in animals with different resistance to hypoxia. The combined using of oxygen insufflation and "KD-234" substance in the experimental ARDS has a similar effect on the enzyme link of antioxidant protection, as in the case of oxygen insufflation, but it significantly increases the antioxidant-prooxidant index in the liver tissues, which is almost returns to the level of control in the groups of MRH- and LRH-animals.Conclusion. In acute respiratory distress syndrome, the combination of oxygen insufflation and the substance "KD-234" due to antioxidant and antiradical properties.

The importance and significance of gene polymorphisms in preeclampsia

2016 ◽  
pp. 45-49
Author(s):  
P.N. Veropotvelyan ◽  
◽  
I.S. Tsehmistrenko ◽  
N.P. Veropotvelyan ◽  
N.S. Rusak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Early Diagnosis ◽  
Gene Polymorphisms ◽  
Individual Risk ◽  
Extended Study ◽  
Stress Genes ◽  
Detoxification System ◽  
The Individual ◽  
The Relationship

Was to conduct a systematic review of data on the relationship between polymorphisms genes of detoxification system and development of preeclampsia (РЕ). Рresents the main genes of detoxification system (GSTPI, GSTМI, GSTТI, GРХI, ЕРНХI, SOD-2, SOD-3, CYPIAL, MTHЕR, MTR) and their functions. Of interest is the possibility of calculating the individual risk of PE based on the results about the presence of a combination of different polymorphisms in the genotype of the female. Question about early diagnosis of РЕ remains controversial and not fully understood. It is necessary to conduct further in-depth, extended study of this problem. Key words: preeclampsia, oxidative stress, genes of the detoxification system.

Hexagonal boron nitride nanoparticles trigger oxidative stress by modulating thiol/disulfide homeostasis

2021 ◽  
pp. 096032712110028
Author(s):  
F Kar ◽  
İ Söğüt ◽  
C Hacıoğlu ◽  
Y Göncü ◽  
H Şenturk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Boron Nitride ◽  
Hexagonal Boron Nitride ◽  
Chemical Properties ◽  
Heart Tissue ◽  
Albino Rats ◽  
Dependent Manner ◽  
Tissue Samples ◽  
Boron Nitride Nanoparticles

Background: Hexagonal boron nitride nanoparticles (hBN NPs) are encouraging nanomaterials with unique chemical properties in medicine and biomedical fields. Until now, the optimal hBN NP’s dosage and biochemical mechanism that can be used for in vivo systems has not been fully revealed. The main aim of this article is to reveal characteristics, serum and tissue interactions and any acute cytotoxic effect of different dose of hBN NPs for the first time. Methods: hBN NPs at concentrations varying between 50–3200 µg/kg was administered by intravenous injection to Wistar albino rats (n = 80) divided into seven dosage and control groups. Blood and tissue samples were taken after 24 hours. Results: Our findings suggested that higher doses hBN NPs caused oxidative stress on the serum of rats dose-dependently. However, hBN NPs did not affect thiol/disulfide homeostasis on kidney, liver, spleen, pancreas and heart tissue of rats. Furthermore, hBN NPs increased serum disulfide formation by disrupting the thiol/disulfide balance in rats. Also, LOOH and MPO levels increased at high doses, while CAT levels decreased statistically. Conclusion: The results revealed that hBN NPs induce oxidative stress in a dose-dependent manner by modulating thiol/disulfide homeostasis in rats at higher concentrations

scholarly journals Downregulation of FeSOD-A expression in Leishmania infantum alters trivalent antimony and miltefosine susceptibility

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Maria Murta Santi ◽  
Paula Alves Silva ◽  
Isabella Fernandes Martins Santos ◽  
Silvane Maria Fonseca Murta
Keyword(s):  
Oxidative Stress ◽  
Western Blot ◽  
Leishmania Infantum ◽  
Transcript Level ◽  
Drug Susceptibility ◽  
Central Component ◽  
Multiplication Rate ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System

Abstract Background Superoxide dismutase (SOD), a central component of the antioxidant defence system of most organisms, removes excess superoxide anions by converting them to oxygen and hydrogen peroxide. As iron (Fe) SOD is absent in the human host, this enzyme is a promising molecular target for drug development against trypanosomatids. Results We obtained Leishmania infantum mutant clones with lower FeSOD-A expression and investigated their phenotypes. Our attempts to delete this enzyme-coding gene using three different methodologies (conventional allelic replacement or two different CRISPR/methods) failed, as FeSOD-A gene copies were probably retained by aneuploidy or gene amplification. Promastigote forms of WT and mutant parasites were used in quantitative reverse-transcription polymerase chain reaction (RT-qPCR) and western blot analyses, and these parasite forms were also used to assess drug susceptibility. RT-qPCR and western blot analyses revealed that FeSOD-A transcript and protein levels were lower in FeSOD-A−/−/+L. infantum mutant clones than in the wild-type (WT) parasite. The decrease in FeSOD-A expression in L. infantum did not interfere with the parasite growth or susceptibility to amphotericin B. Surprisingly, FeSOD-A−/−/+L. infantum mutant clones were 1.5- to 2.0-fold more resistant to trivalent antimony and 2.4- to 2.7-fold more resistant to miltefosine. To investigate whether the decrease in FeSOD-A expression was compensated by other enzymes, the transcript levels of five FeSODs and six enzymes from the antioxidant defence system were assessed by RT-qPCR. The transcript level of the enzyme ascorbate peroxidase increased in both the FeSOD-A−/−/+ mutants tested. The FeSOD-A−/−/+ mutant parasites were 1.4- to 1.75-fold less tolerant to oxidative stress generated by menadione. Infection analysis using THP-1 macrophages showed that 72 h post-infection, the number of infected macrophages and their intracellular multiplication rate were lower in the FeSOD-A−/−/+ mutant clones than in the WT parasite. Conclusions The unsuccessful attempts to delete FeSOD-A suggest that this gene is essential in L. infantum. This enzyme plays an important role in the defence against oxidative stress and infectivity in THP-1 macrophages. FeSOD-A-deficient L. infantum parasites deregulate their metabolic pathways related to antimony and miltefosine resistance. Graphic Abstract

scholarly journals In Vivo Bioavailability of Selenium in Selenium-Enriched Streptococcus thermophilus and Enterococcus faecium in CD IGS Rats

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 463
Author(s):  
Gabriela Krausova ◽  
Antonin Kana ◽  
Marek Vecka ◽  
Ivana Hyrslova ◽  
Barbora Stankova ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Enterococcus Faecium ◽  
Reductase Activity ◽  
Streptococcus Thermophilus ◽  
Heart Tissue ◽  
Sprague Dawley ◽  
Glutathione Reductase Activity ◽  
Novel Concept ◽  
The Individual

The selenium (Se) enrichment of yeasts and lactic acid bacteria (LAB) has recently emerged as a novel concept; the individual health effects of these beneficial microorganisms are combined by supplying the essential micronutrient Se in a more bioavailable and less toxic form. This study investigated the bioavailability of Se in the strains Enterococcus faecium CCDM 922A (EF) and Streptococcus thermophilus CCDM 144 (ST) and their respective Se-enriched forms, SeEF and SeST, in a CD (SD-Sprague Dawley) IGS rat model. Se-enriched LAB administration resulted in higher Se concentrations in the liver and kidneys of rats, where selenocystine was the prevalent Se species. The administration of both Se-enriched strains improved the antioxidant status of the animals. The effect of the diet was more pronounced in the heart tissue, where a lower glutathione reductase content was observed, irrespective of the Se fortification in LAB. Interestingly, rats fed diets with EF and SeEF had higher glutathione reductase activity. Reduced concentrations of serum malondialdehyde were noted following Se supplementation. Diets containing Se-enriched strains showed no macroscopic effects on the liver, kidneys, heart, and brain and had no apparent influence on the basic parameters of the lipid metabolism. Both the strains tested herein showed potential for further applications as promising sources of organically bound Se and Se nanoparticles.

scholarly journals Multiphoton NAD(P)H FLIM reveals metabolic changes in individual cell types of the intact cochlea upon sensorineural hearing loss

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Paromita Majumder ◽  
Thomas S. Blacker ◽  
Lisa S. Nolan ◽  
Michael R. Duchen ◽  
Jonathan E. Gale
Keyword(s):  
Oxidative Stress ◽  
Hearing Loss ◽  
Cell Model ◽  
Organ Of Corti ◽  
Cell Types ◽  
Fluorescence Lifetime Imaging ◽  
Noise Induced Hearing Loss ◽  
Label Free ◽  
Age Related ◽  
The Individual

AbstractAn increasing volume of data suggests that changes in cellular metabolism have a major impact on the health of tissues and organs, including in the auditory system where metabolic alterations are implicated in both age-related and noise-induced hearing loss. However, the difficulty of access and the complex cyto-architecture of the organ of Corti has made interrogating the individual metabolic states of the diverse cell types present a major challenge. Multiphoton fluorescence lifetime imaging microscopy (FLIM) allows label-free measurements of the biochemical status of the intrinsically fluorescent metabolic cofactors NADH and NADPH with subcellular spatial resolution. However, the interpretation of NAD(P)H FLIM measurements in terms of the metabolic state of the sample are not completely understood. We have used this technique to explore changes in metabolism associated with hearing onset and with acquired (age-related and noise-induced) hearing loss. We show that these conditions are associated with altered NAD(P)H fluorescence lifetimes, use a simple cell model to confirm an inverse relationship between τbound and oxidative stress, and propose such changes as a potential index of oxidative stress applicable to all mammalian cell types.

scholarly journals Play Experience as Individual Ability and a Factorof Individual Resistance to Psychosomatic Disorders

2017 ◽  
Vol 13 (4) ◽  
pp. 118-126
Author(s):  
A.V. Serikov
Keyword(s):  
Statistical Analysis ◽  
Cultural Diversity ◽  
Inverse Correlation ◽  
Psychosomatic Disorders ◽  
Individual Resistance ◽  
Significant Inverse Correlation ◽  
Individual Ability ◽  
The Individual ◽  
Play Experience

The paper analyzes the constructs of ‘play experience’ and ‘play experiencing ability’ from the perspective of cultural-historical psychology. The paper stresses the importance of education, play, art, wealth and cultural diversity in the formation of healthy and independent personality. The role of play experience as a healthful factor that allows an individual to acquire resistance to psychosomatic disorders is supported both theoretically and empirically. It is argued that the individual capable of play experience can transform the meaning of a situation (within his/her play experience) and therefore eliminate its psychotraumatic effect which contributes to the development of psychosomatic disorders. The paper provides outcomes of an empirical research with 73 participants (40 female, 33 male; aged 18—45, with the average age of 25 years). The statistical analysis revealed a significant inverse correlation between the level of the individual’s play experiencing ability and the level of his/her somatization (rs = -0,435; p ≤ 0,01), which confirms the research hypothesis.

Sign in / Sign up

Export Citation Format

Share Document