scholarly journals Unravelling peptidomes by in silico mining

Peptidomics ◽  
2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Johannes Koehbach ◽  
Kathryn A.V. Jackson
Keyword(s):  
Plant Extracts ◽  
Biological Samples ◽  
In Silico ◽  
Bioactive Peptides ◽  
Bioinformatics Analysis ◽  
Rapid Identification ◽  
Human Cells ◽  
Domains Of Life ◽  
In Silico Mining

AbstractPeptides of great number and diversity occur in all domains of life and exhibit a range of pharmaceutically relevant bioactivities. The complexity of biological samples including human cells or tissues, plant extracts or animal venom cocktails, often impedes the discovery of novel bioactive peptides using mass spectrometrybased peptidomics analysis. An increasing number of publicly available genome and transcriptome datasets, together with refined bioinformatics analysis, allows for rapid identification of novel peptides which may have been previously unrecognized. Moreover, a combination of information extracted from

In silico mining of WRKY TFs through Solanum melongena L. and Solanum incanum L. transcriptomes and identification of SiWRKY53 as a source of resistance to bacterial wilt

Plant Gene ◽  
2021 ◽  
Vol 26 ◽  
pp. 100278
Author(s):  
Pallavi Mishra ◽  
A.N. Tripathi ◽  
Sarvesh P. Kashyap ◽  
Mohd Aamir ◽  
Kavindra N. Tiwari ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
In Silico ◽  
Solanum Melongena ◽  
Solanum Incanum ◽  
In Silico Mining

scholarly journals In Silico Bioactive Peptide Prediction from The Enzymatic Hydrolysates of Edible Seaweed Rubisco Large Chain

2021 ◽  
Vol 21 (12) ◽  
pp. 615-625
Author(s):  
Ayse Kose
Keyword(s):  
In Silico ◽  
Bioactive Peptides ◽  
Antioxidant Properties ◽  
In Silico Analysis ◽  
Bioactive Peptide ◽  
Bioactive Substances ◽  
Edible Seaweed ◽  
Ic50 Values ◽  
Peptide Prediction ◽  
Ancient Food

Seaweeds are one of the ancient food supplements on Earth. Especially Asian countries use seaweeds as the fundamental ingredient in their cuisine. Seaweeds are photosynthetic organisms living in aquatic ecosystems and in the coastal territories. Seaweeds out of farm areas are frequently observed as coastal wastes. However, seaweeds are outstanding sources for bioactive substances and investigation bioactive properties of seaweed RuBisCO has never been done. RuBisCO is the most abundant protein on Earth but a vast amount of RuBisCO goes through waste. In this study, bioactive peptide prediction of frequently consumed seaweed RuBisCO proteins were analyzed in silico to identify possible bioactive peptides as substitute or support for grain, meat, and dairy based bioactive peptides. A huge portion of peptides were di-, tri- peptides with IC50 values less than 300 µM according to the comparison of BIOPEP database. Including gastric digestion, more than half of the peptides showed DDP-IV and ACE inhibitory activity followed by antioxidant properties. Also, novel antiinflammatory and anti-cancer peptides were found through in silico analysis.

scholarly journals RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

2021 ◽  
Author(s):  
Peiguo Shi ◽  
Michael R Murphy ◽  
Alexis O Aparicio ◽  
Jordan S Kesner ◽  
Zhou Fang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Single Cell ◽  
State Transition ◽  
Cell Types ◽  
Rapid Identification ◽  
Human Cells ◽  
Single Cell Sequencing ◽  
Functional Studies ◽  
Single Marker ◽  
Inhibit Cell Proliferation

While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.

scholarly journals In silico mining of micro-RNAs from Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

2014 ◽  
Vol 13 (1) ◽  
pp. 32-43
Author(s):  
Asokan R. ◽  
K. Roopa H. ◽  
B. Rebijith K. ◽  
H. Ranjitha H. ◽  
K. Krishna Kumar N.
Keyword(s):  
Spodoptera Frugiperda ◽  
In Silico ◽  
Micro Rnas ◽  
Lepidoptera Noctuidae ◽  
In Silico Mining

Porcine myofibrillar proteins as potential precursors of bioactive peptides – an in silico study

2016 ◽  
Vol 7 (6) ◽  
pp. 2878-2885 ◽  
Author(s):  
Paulina Kęska ◽  
Joanna Stadnik
Keyword(s):  
In Silico ◽  
Bioactive Peptides ◽  
Myofibrillar Proteins ◽  
In Silico Study

Selected porcine myofibrillar proteins have been assessed as potential precursors of bioactive peptides based onin silicoanalysis.

PERSPECTIVES OF BIOINFORMATICS FOR RESEARCHING FOOD BIOACTIVE PEPTIDES USING IN SILICO INSTRUMENTS

2020 ◽  
Author(s):  
К.А. РЯЗАНЦЕВА ◽  
Е.Ю. АГАРКОВА
Keyword(s):  
In Silico ◽  
Bioactive Peptides ◽  
Rapid Development ◽  
Biologically Active ◽  
Food Proteins ◽  
Active Peptides ◽  
Biologically Active Peptides ◽  
Speed Up ◽  
Bioinformatic Approaches

В статье приведен обзор основных классических подходов к производству биологически активных пептидов (БП) и способы их идентификации. Показано, что традиционно используемые способы получения и анализа БП требуют значительных временных и материальных затрат, что ограничивает подробные исследования и оперативную разработку БП. Новые биоинформационные подходы in silico, используемые для идентификации, характеристики, разработки биоактивных механизмов и производства БП из пищевых белков, могут упростить получение и исследование БП, что позволит ускорить разработку функциональных продуктов с использованием БП. The article describes the main classical approaches to the production of biologically active peptides and methods for their identification. It has been shown that the traditionally used methods of obtaining and analyzing bioactive peptides (BP) are not only very costly, but also require a significant amount of time, which limits detailed research and rapid development of BP. New bioinformatic approaches in silico used for identification, characterization, development of bioactive mechanisms and production of BP from food proteins can simplify the production and study of biologically active peptides, which will speed up the development of functional products using BP.

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