Reproductive Performance of Rabbit does Artificially Inseminated with Semen Supplemented with GnRH Analogue [des-Gly10, D-Ala6]-LH-RH Ethylamide

2016 ◽  
Vol 19 (3) ◽  
pp. 659-661
Author(s):  
P. Gogol

Abstract The aim of the study was to evaluate, the ability of a GnRH synthetic analogue [des-Gly10, D-Ala6]-LH-RH ethylamide to induce ovulation in rabbit does using intravaginal administration. A total of 138 primiparous lactating does were randomly divided into 4 groups that at the time of insemination received following treatments for ovulation induction: 1 μg of buserelin administered intramuscularly (control group); 5 μg of [des-Gly10, D-Ala6]-LH-RH ethylamide added to the semen dose (D5 group); 10 μg of [des-Gly10, D-Ala6]-LH-RH ethylamide added to the semen dose (D10 group); 15 μg of [des-Gly10, D-Ala6]-LH-RH ethylamide added to the semen dose (D15 group). Kindling rates were 68.8% in D10 and 66.7% in D15 groups and were comparable to that obtained in the control group (72.2%). The kindling rate in group D5 (29.4%) was significantly lower than those recorded in the other groups. The number of live born kits was not significantly affected by the ovulation induction treatment. The results of this study show that [des-Gly10, D-Ala6]-LH-RH ethylamide added directly into the semen dose can effectively stimulate ovulation in rabbits. The dose of 10 μg of [des-Gly10, D-Ala6]-LH-RH ethylamide per doe was sufficient to produce results comparable to those obtained by intramuscular administration of buserelin.

2016 ◽  
Vol 64 (1) ◽  
pp. 116-119 ◽  
Author(s):  
Piotr Gogol

The aim of this study was to evaluate the ability of two synthetic GnRH analogues, goserelin and leuprolide, to induce ovulation in rabbit does using intravaginal administration. A total of 252 primiparous lactating does were randomly divided into five groups that, at the time of insemination, received the following treatments for ovulation induction: 1 µg of buserelin administered intramuscularly (control group), 5 µg of goserelin added to the semen dose (Group G5), 10 µg of goserelin added to the semen dose (Group G10), 5 µg of leuprolide added to the semen dose (Group L5), and 10 µg of leuprolide added to the semen dose (Group L10). The kindling rate was 80.5% in Group G10 and 75.0% in Group L10; these values are comparable to the kindling rate obtained in the control group (85.9%). The kindling rates in Groups G5 and L5 were significantly lower than in the control group (60.0%, 54.2% and 85.9%, respectively). The number of live-born rabbits was not significantly affected by the ovulation induction treatment. As regards the total number of rabbits born the only significant difference was observed between Groups G5 and L5. This study shows the possibility of inducing ovulation in rabbits by adding goserelin and leuprolide directly to the semen dose.


1993 ◽  
Vol 73 (2) ◽  
pp. 287-293
Author(s):  
S. L. Boyles ◽  
L. J. Johnson ◽  
W. D. Slanger ◽  
B. J. Kreft ◽  
J. D. Kirsch

Twenty-six of 52 heifers with an average liveweight of 318 kg were dewormed by intraruminal injection of oxfendazole and placed in drylot on 31 January. The other 26 animals served as controls. All heifers were pastured starting on 14 June and the treatment heifers were dewormed in the same manner as at the start of the experiment (31 January) on 1 July and 31 July. The groups started out with similar total worm eggs per gram of fecal sample, but the dewormed heifers had fewer counts each month thereafter. The drylot average daily gain of the treated heifers was higher (P = 0.01) and less variable (P = 0.12) and by 29 May these animals were heavier by 11.7 kg (P = 0.10). The treated animals did not gain as fast on pasture (P = 0.20), but the gain continued to be less variable (P = 0.09). Age at puberty was not different. Number of open heifers in the treated group was 4 vs. 15% for the control group (P = 0.18). Deworming by intraruminal injection resulted in faster feedlot gains, more uniform gains during both drylot and pasture, reduced parasites and no abscess at injection sites. Key words: Anthelmintics, oxfendazole, cattle, heifers


2021 ◽  
Author(s):  
Ahmed Ismail Damarany

Abstract The current investigation was designed to determine the effect of treatment with anti-parasites (Ivermectin) on mastitis and postpartum reproductive efficiency measurements in Egyptian Baladi cows. Twenty-four of the cows included in this study were split into two groups. The first group (n = 12 cows) was injected two months prepartum with two doses (15 days inter-treatment) of IVOMEC® Plus solution subcutaneously behind the shoulder, 1ml/50kg live body weight as prescribed by the manufacturer, whilst the other group (n = 12 cows) was used as a control group. The present results revealed that the incident rate of mastitis in treated cows’ group was significantly (P < 0.05) lower (16.7%) than control cows’ group (41.7%). The conception rate in treated cows’ group was significantly (P < 0.05) higher (66.7%) compared with (33.3%) in control group. The time from calving to conception in treated cows was significantly (P < 0.05) lower 86.5 ± 11.4 days compared to 123.3 ± 13.2 days in the control group. The occurrence rate of anestrous cases was significantly (P < 0.05) lower (8.3%) in treated cows than (control group) (25%). In conclusion, the current study indicates there is an association between treated cows with anti-parasites (Ivermectin) two months prepartum and incidence rate of mastitis addition an improvement in the reproductive performance in treated cows relative to untreated cows. Therefore, the current study recommends the herdsmen treats their cows with anti-parasites (Ivermectin) during the prepartum period for less mastitis incidence rate and better reproductive efficiency.


2008 ◽  
Vol 52 (No. 9) ◽  
pp. 413-418 ◽  
Author(s):  
S. Cech ◽  
R. Dolezel

Different procedures of treatment of large follicular ovarian cysts in 177 sows using GnRH, hCG and PGF2α are evaluated in this study. Ovarian cysts were diagnosed by transcutaneous ultrasonography, which was a part of routine pregnancy diagnosis. No treatment was performed in the control group (Group 1, <i>n</i> = 29); the method of treatment used in the other groups immediately after the diagnosis was intramuscular administration of lecirelin in doses 50 µg (Group 2, <i>n</i> = 28), 100 µg (Group 3, <i>n</i> = 27) and 200 µg divided into 2 equal doses administered at a 12-hour interval (Group 4, <i>n<i> = 25) and of hCG in doses 1 500 IU (Group 5, <i>n</i> = 23), 3 000 IU (Group 6, <i>n</i> = 21), and 250 µg of cloprostenol (Group 7, <i>n</i> = 24). Insemination rate (IR), conception rate (CR) in inseminated sows, pregnancy rate (PR = recovery rate), treatment-insemination interval (TII) and treatment-pregnancy interval (TPI) within 42 days after the initial examination were evaluated. In addition PR in groups of sows divided according to parity (1–3, 4–6 and ≥ 7) were also evaluated. IR and PR were higher in Group 4 (84.0% and 44.0%) and lower in Group 1 (17.2% and 6.9%) in comparison with the other groups (<i>P</i> < 0.001 and <i>P</i> < 0.05). CR, TII and TPI did not differ between the experimental groups. PR were similar in sows with different parity. The study proved a positive response in sows with large follicular ovarian cysts to the treatment consisting of 2 administrations of 100 µg GnRH at a 12-hour interval.


Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 143
Author(s):  
Marina Bottrel ◽  
Isabel Ortiz ◽  
Manuel Hidalgo ◽  
María Díaz-Jiménez ◽  
Blasa Pereira ◽  
...  

Two prostanglandins (luprostiol, LUP, and dinoprost, DIN) and two ovulation-inducing agents (human Chorionic Gonadotropin, hCG, and deslorelin, DES) were evaluated for luteolysis and estrus induction, and for ovulation induction, respectively, in embryo donor jennies. Twenty-six fertile Andalusian jennies were used. In Experiment 1, jennies (n = 112 cycles) were randomly treated with either LUP or DIN after embryo flushing. In Experiment 2, donors (n = 84 cycles) were randomly treated with either hCG or DES to induce ovulation. No differences were found between prostaglandins for all variables studied (prostaglandin–ovulation interval (POI), interovulatory interval (IOI), embryo recovery rate (ERR), positive flushing rate (PFR) and embryo grade (EG)). The ovulation rate was similar for hCG and DES (60.9% vs. 78.7%). However, the interval to ovulation (ITO) was affected (62.61 ± 7.20 vs. 48.79 ± 2.69 h). None of the other variables studied (ERR, PFR and EG) were affected (p > 0.05), except for embryo quality (p = 0.009). In short, both prostaglandins evaluated are adequate to induce luteolysis and estrus. Both ovulation-inducing agents hastened ovulation, but DES seems to be more effective than hCG. Follicular diameter affected the interval from treatment to ovulation, and high uterine edema was related to low embryo quality.


1999 ◽  
Vol 47 (4) ◽  
pp. 451-460
Author(s):  
Ö. ETLÝK ◽  
V. SAÐMANLIGÝL ◽  
Ý. PÝÞKÝN ◽  
A. Tomur

The aim of this research was to observe the effects of cyclophosphamide and its uroprotective agents, mesna and hyperbaric oxygen (HBO), on the motility of urinary bladder muscle in guinea pigs. In the experimental groups, mesna and cyclophosphamide were intraperitoneally injected at a dose of 21.5 mg/kg and 68.1 mg/kg, respectively. For the combination of mesna and cyclophosphamide, one dose of mesna was injected 20 min before cyclophosphamide administration and three additional injections of mesna were repeated every three hours. A total of 8 HBO exposures were performed at 2.8 ATA for 90 min twice daily for another experimental group. In the HBO and cyclophosphamide combined group 5 HBO exposures were given prophylactically before cyclophosphamide. The combination of mesna, HBO and cyclophosphamide was administered by the same procedure. The contractions obtained in response to acetylcholine (ACh, 10–4M) in the control group were reduced using cyclophosphamide and HBO individually, but not by mesna. However, the contractions belonging to the various combinations of these three agents were not different from those seen in the control group. On the other hand, the combinations of cyclophosphamide, mesna and HBO showed higher responses to ACh than the groups in which cyclophosphamide and HBO were used individually, while the responses elicited by the cyclophosphamide and HBO combination were greater than those seen in the group treated with HBO only.


2011 ◽  
Vol 82 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Rengin Attin ◽  
Bogna Stawarczyk ◽  
Defne Keçik ◽  
Michael Knösel ◽  
Dirk Wiechmann ◽  
...  

Abstract Objective: To compare the influence of demineralized and variously pretreated demineralized enamel on the shear bond strength of orthodontic brackets. Materials and Methods: Sixty bovine enamel specimens were allocated to five groups (n  =  12). Specimens of group 1 were not demineralized and were not pretreated, but served as controls. The other specimens were demineralized to form artificial carious lesions. Samples from group 2 were only demineralized and were kept untreated in artificial saliva. The other samples were pretreated with highly concentrated fluoride preparations (group 3: Elmex Gelee, 1.23% F; group 4: Clinpro White Varnish, 2.23% F) or with an infiltrating resin (group 5: Icon). After respective pretreatments, brackets were adhesively fixed on all specimens with an adhesive system after etching with 35% phosphoric acid and application of a primer and bracket resin cement (Transbond XT). Bracket shear bond strength was evaluated with a universal testing machine. Statistical analysis was performed by one-way analysis of variance followed by a post-hoc Scheffé test. Results: Shear bond strength in control group 1 was statistically significantly greater compared with that in all other groups. Application of the infiltrating resin Icon (group 5) as pretreatment resulted in statistically significantly greater bond strength as compared with pretreatments with fluoride compounds (groups 3 and 4) and treatment provided without pretreatment (group 2). Groups 2, 3, and 4 did not significantly differ from each other. Conclusion: Pretreatment with the infiltrating resin is a beneficial approach to increasing the shear bond strength of brackets to demineralized enamel.


2021 ◽  
Vol 52 (3) ◽  
pp. 535-546
Author(s):  
T. A. Abdulkareem ◽  
S. J. Muhammad ◽  
A. N. Yousif

This study was conducted to examine the effect of Kisspeptin-10 as an alternative to equine chorionic gonadotropin (eCG) in estrus- synchronization protocol for improving the reproductive performance of Karadi ewes. Forty adult ewes of 3-7 years old and 65–70 kg live body weight were equally divided into four equal groups. Animals were inserted with a progestagen (60 mg MAP)-impregnated sponges for 13 days as estrus synchronization protocol. Following withdrawal of sponges, first group was served as control (C) and intramuscularly injected with normal saline only. The second group was intramuscularly injected with 250 IU of eCG (eCG), while those of third and fourth groups were intravenously injected with 4 and 8 µg /kg body weight of Kisspeptin-10, referred as Kisspeptin1 and Kisspeptin2 groups respectively. Serum progesterone and LH concentrations did not significantly differ among groups before estrus synchronization protocol and eCG or Kisspeptin treatments. The two Kisspeptin-injected groups attained better (P≤0.01) overall mean estrus (100%), fertility (90%), conception (90 and 100%, respectively), lambing (90%), and twinning (10%) rates with lesser barrenness (10%) as compared with the other groups. Moreover, eCG and Kisspeptin groups exhibited greater (P≤ 0.01) percentage of increasing fecundity (233%) and extra lambs born (0.7) than the control group. In conclusion, treatment of Karadi ewes either with Kisspeptin-10 or eCG hormones enhanced the overall reproductive performance, but did not affect serum progesterone and LH concentrations.


2018 ◽  
Vol 30 (1) ◽  
pp. 173
Author(s):  
F. Y. Hilari ◽  
J. C. Villanueva ◽  
W. F. Huanca ◽  
B. Lira ◽  
W. Huanca

Experiments were designed to determine the effect of ovulation stimulus on the biochemical composition of oviduct fluid in alpacas (Vicugna pacos). Twenty-four adult females were used to determine profiles of protein, albumin, glucose, cholesterol, and triglycerides in oviduct fluid. The animals were stimulated with 1 mL of seminal plasma (SP), 0.042 mg of a gonadotropin-releasing hormone (GnRH) analogue (buserelin acetate; Conceptal, Intervet, Holland), or PBS as control group. The oviduct was removed inmediately after slaughter on Day 2 or 4 after ovulation induction and washed with 3 mL of PBS solution. Determination of the biochemical composition was carried out with specific kits from the FAR Diagnostics Italia laboratory using a semiautomatic biochemical analyzer of the Sinowa China brand, which is based on spectrophotometric evaluation. The results are presented in Table 1. Our results suggest that the method of induction of ovulation affected the biochemical composition of oviduct fluid, including concentrations of albumin, cholesterol, total protein, triglyceride, and glucose. Table 1.Biochemical composition of oviduct fluid (mean ± SEM).


2017 ◽  
Vol 57 (8) ◽  
pp. 1725
Author(s):  
Pan Zhou ◽  
Guangbo Luo ◽  
Lianqiang Che ◽  
Yan Lin ◽  
Shengyu Xu ◽  
...  

The aim of this study was to determine the effect of supplementing different concentrations of extruded full-fat soybean (EFS) to corn-soybean meal-based lactation diets, at the expense of soybean meal and oil, on sow and litter performance. During gestation, a total of 60 Landrace × Yorkshire multiparous sows were fed the same gestational diet. After farrowing, sows were randomly allotted to one of four treatments including Control (corn-soybean meal-soybean oil), and 6.1%, 12.2%, and 18.3% EFS diets, until Day 21 of lactation. Individual weight at weaning tended to be heavier in the 12.2% EFS group as compared with Controls (P = 0.07). Litter weight in 12.2% EFS group was significantly increased compared with Controls, representing an increase of 7.8%. Average daily feed intake of sows in the 12.2% EFS group tended to increase (P = 0.08) when compared with the other three groups. At Day 21 of lactation, fat and protein content in the milk of the 12.2% EFS group were higher than those of the Control group (P &lt; 0.05). In conclusion, these results demonstrated that feeding lactating sows diets supplemented with EFS had a positive effect on milk composition as well as litter performance. Among the three dietary levels of EFS tested, 12.2% EFS supplementation level showed preferable reproductive performance.


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