Cerebrospinal Fluid Levels of Neopterin in Child Patients with Neurological Diseases-In Correlation with IFN-γ or TNF-α

Pteridines ◽  
1999 ◽  
Vol 10 (1) ◽  
pp. 27-31
Author(s):  
Yasuhiko Kawakami ◽  
Yoshitaka Fukunaga ◽  
Kentaro Kuwabara ◽  
Takehisa Fujita ◽  
Osamu Fujino ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Neurological Diseases ◽  
Febrile Convulsion ◽  
Reactive Protein ◽  
Tnf Α ◽  
Child Patients ◽  
The Central Nervous System ◽  
Ifn Γ ◽  
Fluid Levels

Cerebrospinal fluid (CSF) neopterin levels were measured in child patients with neurological diseases. CSF neopterin levels on admission were significantly higher in patients with bacterial meningitis than in those with aseptic meningitis or in those with non-pleocytotic CSF. CSF neopterin levels in patients with bacterial meningitis one day after admission were higher than those on admission and were markedly elevated with high levels of CSF IFN-γ and TNF-α. CSF neopterin levels in patients with bacterial meningitis one day after admission were related to the period of positive serum C-reactive protein. In patients with non-pleocytotic CSF, CSF neopterin and CSF IFN-y levels were higher in patients with febrile convulsion (FC) than in those with pyrexia only or convulsion (including epilepsy) only. These results suggest that elevation of CSF neopterin in bacterial meningitis results from monocytes/ macrophages costimulated with IFN-γ and TNF-α and that immunological activation in the central nervous system is one of the mechanisms of onset with FC.

Correlation between Neopterin, Biopterin and Nitrite/Nitrate in Cerebrospinal Fluid in Child Patients with Neurological Diseases

Pteridines ◽  
2003 ◽  
Vol 14 (1) ◽  
pp. 5-8
Author(s):  
Yasuhiko Kawakami ◽  
Mayuko Sakamoto ◽  
Ken-ichi Shimada ◽  
Eiji Noguchi ◽  
Kentaro Kuwabara ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Aseptic Meningitis ◽  
Neurological Diseases ◽  
Febrile Convulsions ◽  
Child Patients ◽  
Nitrite Nitrate

Abstract Cerebrospinal fluid (CSF) neopterin been previously reported in various diseases. In this study CSF neopterin, biopterin, and nitrite/nitrate (NOx) Contents were measured and the correlation between them in child patients with various kinds of neurological diseases were investigated. Changes in the CSF neopterin levels in patients with bacterial meningitis were similar to those previously reported for those with bacterial meningitis; on the 2th hospital day they were significantly higher than on admission, and on the 6th hospital day they were tapered. The CSF biopterin levels and CSF NOx content in patients with bacterial meningitis on admission were significantly higher than those with other categories and were decreased gradually. Although patients with high levels of CSF neopterin tended to have high CSF biopterin levels in any categories, there was no significant correlation between CSF neopterin and biopterin levels. The CSF biopterin and NOx levels in patients with convulsions were higher than those with aseptic meningitis. Since the neuro-protective or anticonvulsant role for NO was previously reported, high CSF biopterin and NOx levels in patients having epilepsy or febrile convulsions may be regarded as one of the endogenous mechanisms for recovery from an overexcitatory brain in patients with convulsive diseases.

Neopterin and Cytokines in Non-pleocytotic Cerebrospinal Fluid in Child Patients

Pteridines ◽  
2000 ◽  
Vol 11 (4) ◽  
pp. 142-146
Author(s):  
Yasuhiko Kawakami ◽  
Kentaro Kuwabara ◽  
Takehisa Fujita ◽  
Osamu Fujino ◽  
Yoshitaka Fukunaga
Keyword(s):  
Cerebrospinal Fluid ◽  
Immune Activation ◽  
Serum Levels ◽  
Microglial Cells ◽  
Febrile Convulsions ◽  
Exanthem Subitum ◽  
Tnf Α ◽  
Child Patients ◽  
Ifn Γ

Abstract CSF and serum levels of neopterin and several kinds of CSF cytokines were measured in child patients with non-pleocytotic CSF. The CSF neopterin levels with febrile convulsions (FCs) were 27.4±33.0 nmol/1 and the CSF neopterinlserum neopterin ratio (C/S ratio) with FCs was 2.07±2.06. The longer the FCs patients' convulsions lasted, the higher their CSF neopterin levels tended to be come. The CSF neopterin levels with FCs were significantly higher than in those with pyrexia without convulsions (6.5±2.5 nmol/L) or convulsions without pyrexia, including epilepsy (4.9±2.9 nmol/L). The C/S ratio was also higher in patients with FCs than in those with pyrexia without convulsions (0.31±0.17) or convulsions without pyrexia (0.82±0.50).In addition, there was a tendency for CSF IFN-γ levels to be higher in patients with FCs than in those with pyrexia without convulsions or convulsions without pyrexia. However, TNF-α and IL-1α were under measurable levels in cases of non-pi eocytotic CSF. The CSF neopterin levels in patients with exanthem subitum with FCs were higher than in those without FCs. It has been reported that there is a possibility of neopterin production by microglial cells. Our finding that CSF neopterin levels were elevated in patients with FCs, whose CSF had no pleocytosis, are consistent with the possibility of neopterin production by microglia. Our results further suggest that immune activation of microgrial cells is one of the mechanisms involved in the onset of FCs.

scholarly journals Estimation of Some Interleukins in Cerebrospinal Fluid in Children with Meningitis

2019 ◽  
Vol 12 (04) ◽  
pp. 2151-2155
Author(s):  
Entedhar R. Sarhat ◽  
Zubaidah N. M Albarzanji ◽  
Chateen I. Ali Pambuk
Keyword(s):  
Cerebrospinal Fluid ◽  
Serum Glucose ◽  
Control Group ◽  
Serum Total Protein ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Tnf Α ◽  
The Central Nervous System ◽  
Factor Alpha ◽  
Necrosis Factor

Meningitis is often associated with cerebral compromise which may be responsible for neurological squeal in nearly half of the survivors. Little is known about the mechanisms of CNS involvement in bacterial meningitis. the current study was to analyze the clinical significance of cerebrospinal fluid (CSF) concentrations of interleukin (IL)- 6, IL-1, IL-8, IL-10, tumor necrosis factor-alpha (TNF-α), and C-reactive protein in children with meningitis (n=35) and compared with control subjects (n=28). Serum total protein, interleukine-6, IL-1 , IL-8, IL-10 , and, CRP, and TNF-α levels were found to be significantly high in study group, whereas serum glucose IL-10 levels were significantly low comparing with control group. Positive correlation was observed between IL-6with glucose , CRP , and IL-8 , also between IL-10 with protein , TNF with IL-8 ,and IL-1 with CRP . whereas there was negative correlation between IL-6 with protein , TNF , IL-1 , IL-10 , also between IL-1 with IL-8 ,between IL-10 with, and TNF , IL-10 with glucose . More studies performed in suitable models of meningitis are required in order to establish the routine use of inflammatory markers in the diagnosis of infectious diseases of the central nervous system

scholarly journals Interleukin-6 and granulocyte macrophage-csf in the cerebrospinal fluid from hiv infected subjects with involvement of the central nervous system

1992 ◽  
Vol 50 (2) ◽  
pp. 180-182 ◽  
Author(s):  
O. Perrella ◽  
M. Guerriero ◽  
E. Izzo ◽  
M. Soscia ◽  
P. B. Carrieri
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Interleukin 6 ◽  
Neurological Diseases ◽  
Aids Dementia Complex ◽  
Gm Csf ◽  
Aids Dementia ◽  
High Incidence ◽  
The Central Nervous System ◽  
Csf Levels

We detected the cytokines interleukin-6 (IL-6) and granulocyte macrophage-CSF (GM-CSF) by ELISA in the CSF and serum of 30 HIV-infected patients classified as AIDS dementia complex (ADC), and 20 subjects with other neurological diseases (OND). We have found a high incidence of detectable IL-6 and GM-CSF in the CSF of ADC patients compared with OND patients. No statistical differences were observed between both groups for serum IL-6 and GM-CSF levels. These results suggest an intrathecal synthesis of these cytokines and a possible involvement in the pathogenesis of ADC.

Brain-blood permeability: TNF-α promotes escape of protein tracer from CSF to blood

2000 ◽  
Vol 279 (1) ◽  
pp. R148-R151 ◽  
Author(s):  
Jodi B. Dickstein ◽  
Harvey Moldofsky ◽  
John B. Hay
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Tumor Necrosis ◽  
Venous Blood ◽  
Protein Clearance ◽  
Tracer Recovery ◽  
Tnf Α ◽  
The Central Nervous System ◽  
Necrosis Factor

The objective of this study was to determine the effect of tumor necrosis factor (TNF)-α on the efflux of protein from the central nervous system to blood based on assessing the clearance of radiolabeled albumin from the cerebrospinal fluid (CSF) to blood in rats. 125I-labeled human serum albumin (125I-HSA) was injected into a lateral ventricle, and venous blood was sampled hourly to determine the basal CSF protein clearance into the blood. After this, rats were intraventricularly infused with 10 μl TNF-α and 10 μl131I-HSA ( n = 6) or 10 μl saline and 10 μl 131I-HSA ( n = 6). Venous blood was sampled hourly for 3 h. 131I-HSA tracer recovery increased threefold in the venous blood and was significantly higher in the spleen, muscles, and skin in animals treated with TNF-α. No significant changes were observed in control animals treated with saline. The data suggest that TNF-α promotes the clearance of protein macromolecules from the CSF to the venous blood.

scholarly journals Inflammatory and immunogenetic markers in correlation with pulmonary tuberculosis

2013 ◽  
Vol 39 (6) ◽  
pp. 719-727 ◽  
Author(s):  
Beatriz Lima Alezio Muller ◽  
Daniela Maria de Paula Ramalho ◽  
Paula Fernanda Gonçalves dos Santos ◽  
Eliene Denites Duarte Mesquita ◽  
Afranio Lineu Kritski ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Pulmonary Tuberculosis ◽  
Gene Polymorphisms ◽  
Serum Levels ◽  
Acute Inflammatory Response ◽  
Reactive Protein ◽  
Antituberculosis Treatment ◽  
Tnf Α ◽  
Referral Hospitals ◽  
Ifn Γ

OBJECTIVE: To describe serum levels of the cytokines IL-10, TNF-α, and IFN-γ, as well as polymorphisms in the genes involved in their transcription, and their association with markers of the acute inflammatory response in patients with pulmonary tuberculosis.METHODS: This was a descriptive, longitudinal study involving 81 patients with pulmonary tuberculosis treated at two referral hospitals. We collected data on sociodemographic variables and evaluated bacteriological conversion at the eighth week of antituberculosis treatment, gene polymorphisms related to the cytokines studied, and serum levels of those cytokines, as well as those of C-reactive protein (CRP). We also determined the ESR and CD4+ counts.RESULTS: The median age of the patients was 43 years; 67 patients (82.7%) were male; and 8 patients (9.9%) were infected with HIV. The ESR was highest in the patients with high IFN-γ levels and low IL-10 levels. IFN-γ and TNF-α gene polymorphisms at positions +874 and −238, respectively, showed no correlations with the corresponding cytokine serum levels. Low IL-10 levels were associated with IL-10 gene polymorphisms at positions −592 and −819 (but not −1082). There was a negative association between bacteriological conversion at the eighth week of treatment and CRP levels.CONCLUSIONS: Our results suggest that genetic markers and markers of acute inflammatory response are useful in predicting the response to antituberculosis treatment.

scholarly journals 1104 QUANTITATIVE MEASUREMENT OF C-REACTIVE PROTEIN (CRP) IN CEREBROSPINAL FLUID (CSF) OF PATIENTS WITH BACTERIAL MENINGITIS

1985 ◽  
Vol 19 (4) ◽  
pp. 294A-294A
Author(s):  
Barry M Gray ◽  
Don Simmons ◽  
Henry F Mason ◽  
Scott K Barnum ◽  
John E Volanakis
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Quantitative Measurement ◽  
C Reactive Protein ◽  
Reactive Protein

Elevated cerebrospinal fluid levels of manganese superoxide dismutase in bacterial meningitis

1995 ◽  
Vol 131 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Yoshikiyo Hirose ◽  
Kenji Mokuno ◽  
Masakazu Wakai ◽  
Akira Takahashi ◽  
Yoshio Hashizume ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Superoxide Dismutase ◽  
Bacterial Meningitis ◽  
Manganese Superoxide Dismutase ◽  
Cerebrospinal Fluid Levels ◽  
Manganese Superoxide ◽  
Fluid Levels

Cerebrospinal fluid levels of chemokines in HIV infected patients with and without opportunistic infection of the central nervous system

2009 ◽  
Vol 287 (1-2) ◽  
pp. 79-83 ◽  
Author(s):  
Paulo Pereira Christo ◽  
Márcia de Carvalho Vilela ◽  
Thales Lage Bretas ◽  
Renan Barros Domingues ◽  
Dirceu Bartolomeu Greco ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Opportunistic Infection ◽  
Cerebrospinal Fluid Levels ◽  
The Central Nervous System ◽  
Fluid Levels

scholarly journals Comparison of Seven Formulae and Isoelectrofocusing for Determination of Intrathecally Produced IgG in Neurological Diseases

1992 ◽  
Vol 29 (4) ◽  
pp. 405-410 ◽  
Author(s):  
Sten Öhman ◽  
Jan Ernerudh ◽  
Pia Forsberg ◽  
Annemarie Henriksson ◽  
Henning von Schenck ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Reference Group ◽  
Neurological Diseases ◽  
Diagnostic Sensitivity ◽  
Reference Limits ◽  
The Central Nervous System ◽  
Positive Results

Seven different formulae and agarose isoelectrofocusing (AIF) using immunolabelling for IgG were compared for their ability to discriminate between intrathecally produced IgG and transudated IgG in cerebrospinal fluid. All reference limits were set to a specificity of 97·5% (reference group, n = 211). The probability of a positive test ( p+) was evaluated for 112 patients with multiple sclerosis (MS), 42 with meningitis, 114 with noninflammatory diseases affecting the central nervous system (CNS), 23 with Guillain-Barré syndrome, and 56 with various diseases not affecting the CNS. Agarose isoelectrofocusing had the best diagnostic sensitivity (93%) for MS, combined with a low p+ (0–19%) for other diseases. Among the formulae, the IgG extended index and Reiber's hyperbolic formula were equivalent, giving high (75–79%) diagnostic sensitivity for MS combined with low p+ (4–22%) for other diseases. All other formulae, although sensitive for MS, had a higher rate of false positive results.

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