Chemical Constituents of Pedicularis densispica Franch

An ethanol extract of the dried aerial of Pedicularis densispica Franch (Scrophulariaceae) afforded a new lignan, densispicoside (1), and two new iridoids, densispicnin C and D (14, 15), together with four lignans, (+)-isolarisiresinol 3a-O-β -D-glucopyranoside (2), (-)-pinoresinol-β - D-glucopyranoside (3), syringaresinol mono-β -D-glucopyranoside (4), longifloroside B (5), eight phenylpropanoids, 4-O-β -D-glucopyranosyl-sinapic acid methyl ester (6), 3-(4-hydroxy-3-methoxyphenyl)- 1,2,3-propantriol (7), citrusin C (8), robustaside B (9), verbascoside (10), martynoside (11), 2ʺ-O-acetylverbascoside (12), cis-martynoside (13), and two iridoid glycosides, shanzhiside methyl ester (16), and 8-epiloganin (17). Their structures were established on the basis of chemical and spectroscopic studies. Bioactivity results indicate that P. densispica shows activity on PAI-1 antithrombus assay in vitro and antifatigue activities in vivo.

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The Genus Lagochilus (Lamiaceae): A Review of Its Diversity, Ethnobotany, Phytochemistry, and Pharmacology

Plants ◽

10.3390/plants10010132 ◽

2021 ◽

Vol 10 (1) ◽

pp. 132

Author(s):

Nilufar Z. Mamadalieva ◽

Davlat Kh. Akramov ◽

Ludger A. Wessjohann ◽

Hidayat Hussain ◽

Chunlin Long ◽

...

Keyword(s):

Secondary Metabolites ◽

Chemical Constituents ◽

Iridoid Glycosides ◽

Herbal Medicines ◽

Future Research ◽

South Central ◽

Pharmacological Studies ◽

Central South

The genus Lagochilus (Lamiaceae) is native to Central, South-Central, and Eastern Asia. It comprises 44 species, which have been commonly used as herbal medicines for the treatments of various ailments for thousands of years, especially in Asian countries. This review aims to summarize the chemical constituents and pharmacological activities of species from the genus Lagochilus to unveil opportunities for future research. In addition, we provide some information about their traditional uses, botany, and diversity. More than 150 secondary metabolites have been reported from Lagochilus, including diterpenes, flavonoids, phenolic compounds, triterpenoids, iridoid glycosides, lignans, steroids, alkaloids, polysaccharides, volatile, non-volatile and aromatic compounds, lipids, carbohydrates, minerals, vitamins, and other secondary metabolites. In vitro and in vivo pharmacological studies on the crude extracts, fractions, and isolated compounds from Lagochilus species showed hemostatic, antibacterial, anti-inflammatory, anti-allergic, cytotoxic, enzyme inhibitory, antispasmodic, hypotensive, sedative, psychoactive, and other activities.

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High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Molecules ◽

10.3390/molecules26133977 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3977

Author(s):

Shaoyun Wang ◽

Xiaozhu Sun ◽

Shuo An ◽

Fang Sang ◽

Yunli Zhao ◽

...

Keyword(s):

High Performance ◽

Chemical Constituents ◽

Water Extract ◽

Ethanol Extract ◽

In Vivo Studies ◽

Polygonum Multiflorum ◽

Q Exactive ◽

Orbitrap Ms

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

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Phytochemistry and Pharmacology of the Genus Pedicularis Used in Traditional Chinese Medicine

The American Journal of Chinese Medicine ◽

10.1142/s0192415x14500670 ◽

2014 ◽

Vol 42 (05) ◽

pp. 1071-1098 ◽

Cited By ~ 13

Author(s):

Mao-Xing Li ◽

Xi-Rui He ◽

Rui Tao ◽

Xinyuan Cao

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Chemical Constituents ◽

Iridoid Glycosides ◽

In Vivo Studies ◽

Chemical Components ◽

Wide Range ◽

Phenylpropanoid Glycosides

In the present review, the literature data on the chemical constituents and biological investigations of the genus Pedicularis are summarized. Some species of Pedicularis have been widely applied in traditional Chinese medicine. A wide range of chemical components including iridoid glycosides, phenylpropanoid glycosides (PhGs), lignans glycosides, flavonoids, alkaloids and other compounds have been isolated and identified from the genus Pedicularis. In vitro and in vivo studies indicated some monomer compounds and extracts from the genus Pedicularis have been found to possess antitumor, hepatoprotective, anti-oxidative, antihaemolysis, antibacterial activity, fatigue relief of skeletal muscle, nootropic effect and other activities.

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PPARα/γ, adiponectin and GLUT4 overexpression induced by moronic acid methyl ester influenced on glucose and triglycerides levels of experimental diabetic mice

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2021-0526 ◽

2021 ◽

Author(s):

Samuel Estrada-Soto ◽

Litzia Cerón-Romero ◽

Gabriel Navarrete-Vázquez ◽

Edgar Rosales-Ortega ◽

Jaime H. Gómez-Zamudio ◽

...

Keyword(s):

Methyl Ester ◽

Acid Methyl Ester ◽

Control Group ◽

Oral Glucose ◽

Acute Administration ◽

Diabetic Mice ◽

Ppar Γ ◽

Acid Methyl

The current study aimed to determine the antidiabetic and antidyslipidemic activities of moronic acid methyl ester (1) by in vivo, in vitro, in silico and molecular biology studies. Compound 1 was evaluated to establish its dose-dependent antidiabetic and antihyperglycemic (50 mg/kg) activities, in diabetic and normoglycemic male CD1 mice, respectively. Also, compound 1 was subjected to a sub-acute study (50 mg/kg/day for eight days) to determine blood biochemical profiles and the expression of PTP-1B, GLUT4, PPAR-α, PPAR-γ, adiponectin, IL-1β, and MCP1 in adipose tissue of animals after treatment. Different doses in acute administration of 1 decreased glycemia (p < 0.05), compared with vehicle, showing greater effectiveness in the range 50-160 mg/kg. Also, the oral glucose tolerance test (OGTT) showed that 1 induced a significant antihyperglycemic action by opposing the hyperglycemic peak (p < 0.05). Moreover, 1 subacute administration decrease glucose and triglycerides levels after treatment (p < 0.05); while the expression of PPAR-α and γ, adiponectin and GLUT4 displayed an increase (p< 0.05) compared with the diabetic control group. In conclusion, compound 1 showed antihyperglycemic, antidiabetic and antidyslipidemic effects in normal and diabetic mice, probably due to insulin sensitization through increase mRNA expression of GLUT4, PPAR-α, PPAR-γ and adiponectin genes.

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Chlorogenic acid methyl ester exerts strong anti-inflammatory effectsviainhibiting the COX-2/NLRP3/NF-κB pathway

Food & Function ◽

10.1039/c8fo01281d ◽

2018 ◽

Vol 9 (12) ◽

pp. 6155-6164 ◽

Cited By ~ 12

Author(s):

Lang Zhang ◽

Ya Fan ◽

Hanwen Su ◽

Li Wu ◽

Yuying Huang ◽

...

Keyword(s):

Methyl Ester ◽

Chlorogenic Acid ◽

Acid Methyl Ester ◽

Acid Methyl ◽

Cox 2 ◽

Anti Inflammatory

In vivoandin vitrostudies show that chlorogenic acid methyl ester (CME) has been proven to be a potential nutraceutical for preventing inflammation.

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Chemical Constituents and Antidepressant-Like Effects in Ovariectomized Mice of the Ethanol Extract of Alternanthera philoxeroides

Molecules ◽

10.3390/molecules23092202 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2202 ◽

Cited By ~ 3

Author(s):

Charinya Khamphukdee ◽

Orawan Monthakantirat ◽

Yaowared Chulikhit ◽

Suradet Buttachon ◽

Michael Lee ◽

...

Keyword(s):

Crude Extract ◽

Estrogenic Activity ◽

Chemical Constituents ◽

Corticosterone Level ◽

Ethanol Extract ◽

Alternanthera Philoxeroides ◽

Ovariectomized Mice ◽

Propenoic Acid

The previously unreported flavone glycoside, demethyltorosaflavone B (2) and the E-propenoic acid substituted flavone, torosaflavone E (3a), were isolated together with nine previously reported metabolites, including indole-3-carbaldehyde, oleanonic acid, vanillic acid, p-hydroxybenzoic acid, altheranthin (1a), alternanthin B (1b), demethyltorosaflavone D (3b), luteolin 8-C-E-propenoic acid (4) and chrysoeriol 7-O-rhamnoside (5), from the ethanol extract of the aerial part of Althernanthera philoxeroides. The crude ethanol extract was evaluated for its in vitro estrogenic activity in MCF-7 breast cancer cell line. The crude ethanol extract was also investigated in vivo for its antidepressant-like effects on ovariectomized mice using tail suspension and forced swimming tests, while its effect on the locomotor activity was evaluated by a Y-maze test. The effect of the crude extract on the serum corticosterone level, size and volume of uterus of the ovariectomized mice were also investigated. The expression of the mouse cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB), brain-derived neurotrophic factor (BDNF) and β-actin mRNAs in hippocampus and frontal cortex was also evaluated, using semiquantitative reverse transcription-polymerase chain reaction. The crude extract and the isolated compounds 1a, 1b, 3a, 3b and 5, were evaluated for their inhibitory effects on monoamine oxidases (MAOs)-A and -B.

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Bioactive Variability and In Vitro and In Vivo Antioxidant Activity of Unprocessed and Processed Flour of Nine Cultivars of Australian lupin Species: A Comprehensive Substantiation

Antioxidants ◽

10.3390/antiox9040282 ◽

2020 ◽

Vol 9 (4) ◽

pp. 282 ◽

Cited By ~ 2

Author(s):

Kishor Mazumder ◽

Afia Nabila ◽

Asma Aktar ◽

Asgar Farahnaky

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Bioactive Compounds ◽

Antioxidant Activities ◽

Acid Methyl Ester ◽

Heat Processing ◽

Acid Methyl ◽

Ms Analysis

The aim of this present investigation was to analyze bioactive compounds, as well as demonstrate the antioxidant activities of nine cultivars of Australian lupin species accompanied by observing the effect of domestic heat processing on their antioxidant activities adopting in vivo and in vitro approaches. Gas chromatography mass spectroscopy (GC-MS) analysis was performed for profiling bioactive compounds present in lupin cultivars. Multiple assay techniques involving quantification of polyphenolics, flavonoids and flavonol, electron transfer (ET) based assay, hydrogen atom transfer (HAT)-based assay and in vivo assays were performed. The major compounds found were hexadecanoic acid methyl ester, 9,12-octadecadienoic acid methyl ester, methyl stearate, lupanine,13-docosenamide and 11-octadecenoic acid (Z)- methyl ester. Mandelup was found to show excellent antioxidant activity. Moreover, Jurien, Gunyidi and Barlock had strong antioxidant activity. Both positive and negative impacts of heat processing were observed on antioxidant activity. Heating and usage of excess water during processing were the key determinants of loss of antioxidants. Negligible loss of antioxidant activity was observed in most of the assays whereas inhibition of both lipid peroxidation (33.53%) and hemolysis of erythrocytes (37.75%) were increased after processing. In addition, in vitro and in vivo antioxidant assays are found to show statistically significant (* p < 0.05 and ** p < 0.01) results, which are supported by the presence of a number of antioxidant compounds in GC-MS analysis.

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Antidiarrheal Activity of Ethanol Extract of Ophioglossum Vulgatum In Mice And Spasmolytic Effect on Smooth Muscle Contraction of Isolated Jejunum In Rabbits

10.21203/rs.3.rs-113816/v1 ◽

2020 ◽

Author(s):

Jianwu Zhang ◽

Lan Yang ◽

Yingying Fang ◽

Jing Li ◽

Chunxia Gu ◽

...

Keyword(s):

Smooth Muscle ◽

High Performance ◽

Chemical Constituents ◽

Ethanol Extract ◽

Gastrointestinal Diseases ◽

Control Group ◽

Response Curves ◽

Antidiarrheal Activity

Abstract BackgroundOphioglossum vulgatum Linn. (Ophioglossaceae) (OV), which is traditionally used on wounds and burns, enjoys a reputation as the king of medicine in Taiwan. There are few studies on its role in gastrointestinal diseases. Our aim was to assess the antidiarrheal and spasmolytic effect of the ethanol whole plant extract of Ophioglossum vulgatum (EWOV).MethodsStudy was conducted from June 2018 to July 2019. The chemical constituents of EWOV were analyzed by high performance liquid chromatography (HPLC). In vivo, the antidiarrheal activity of EWOV (125, 250 and 500 mg/kg; orally) in castor oil-induced Kun Ming mice was evaluated. In vitro, the effect of EWOV (0.01-10 mg/mL) on the spontaneous contraction of isolated rabbit jejunum smooth muscle was studied. Verapamil was the positive control group in both vivo and vitro studies. The jejunum stripes were pre-contracted by ACh (10-5 M) and KCl (60 mM) which could induce the jejunum spasm. The possible spasmolytic effect was analyzed in the pretreatment of the jejunum preparations with EWOV (0.3, 1 mg/mL) or verapamil (0.03, 0.1 µM) in Ca2+-free and high-K+ (60 mM) solution containing EDTA.ResultsEWOV (250 and 500 mg/kg) exhibited antidiarrheal effect. EWOV (0.01-10 mg/mL) inhibited the spontaneous and ACh/KCl-induced contraction with an EC50 value of 1.46 (0.89-2.04), 1.06 (0.63-1.48) and 0.48 (0.29-0.67), and it shifted the concentration-response curves of CaCl2 to right with decreased in max, similar to verapamil. ConclusionsEWOV has significant antidiarrheal and spasmolytic effect, possibly by mediating calcium channel blocking activity, this provides the pharmacological basis for use in gastrointestinal disorders.

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