Antioxidant Activity of Flavones from Scutellaria baicalensis in Lecithin Liposomes

1997 ◽  
Vol 52 (11-12) ◽  
pp. 817-823 ◽  
Author(s):  
Janina Gabrielska ◽  
Jan Oszmiańsk ◽  
Romuald Żyłka ◽  
Małgorzata Komorowska
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Skin Diseases ◽  
Inhibitory Effect ◽  
Scutellaria Baicalensis ◽  
Butylated Hydroxytoluene ◽  
Protective Effects ◽  
Liposome Membrane ◽  
Beneficial Effects ◽  
Phosphatidylcholine Liposome

Abstract Trihydroxyflavones of Scutellaria baicalensis, Antioxidant Activity, Liposome, Peroxidation, MDA The antioxidant effect of a trihydroxyflavone extract from Scutellaria baicalensis on oxida­ tion induced by ultraviolet light, was studied with phosphatidylcholine liposome membrane. Also, as standards, the antioxidative activity of baicalin, wogonin, baicalein and butylated hydroxytoluene (BHT) was investigated. Comparison of the protective effects of thecom­pounds studied against photoinduced lipid peroxidation in lecithin liposome membranes showed that: (1) the inhibitory effect of those compounds (at 1.2 mol% antioxidant content in liposomes) on TBA reactive materials from lipid peroxidation decreased in the order of baicalin > BHT ≅ Scutellaria baicalensis. These were found much greater than wogonin and baicalein; (2) the depressed effect of those compounds (at 1.1 mol% compounds content in liposomes) on the production of conjugated dienes (proportional to oxidation index) could be classified as follows: Scutellaria baicalensis ≅ baicalin > BHT, these three were found more active much greather than baicalein and wogonin. Results obtained by ESR measure­ment confirm that Scutellaria baicalensis extract and the BHT compound significantly de­ pressed the effect of liposome oxidation. It was found that the new trihydroxyflavones of Scutellaria baicalensis, ensured a very satisfactory concentration-dependent protection of the liposome membrane against UV-induced oxidation. These findings suggest that some of the beneficial effects of the extract of the Scutellaria baicalensis can be mediated in certain diseases (for example in skin diseases) by their ability to scavenge free radicals and by their protective effect on lipid peroxidation caused by sunlight irradiation.

scholarly journals Implications for Degenerative Disorders: Antioxidative Activity, Total Phenols, Flavonoids, Ascorbic Acid, β-Carotene and β-Tocopherol inAloe vera

2009 ◽  
Vol 2 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Nurten Ozsoy ◽  
Eda Candoken ◽  
Nuriye Akev
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological Effects ◽  
Aloe Vera ◽  
Total Phenols ◽  
Beneficial Effects ◽  
Degenerative Disorders ◽  
Phosphatidylcholine Liposome ◽  
Aqueous Leaf Extract ◽  
Β Carotene

In order to demonstrate whether the known biological effects ofAloe vera(L.) Burm. fil. could correlate with the antioxidant activity of the plant, the antioxidant activity of the aqueous leaf extract was investigated. The present study demonstrated that the aqueous extract fromA. veraleaves contained naturally occuring antioxidant components, including total phenols, flavonoids, ascorbic acid, β-carotene and α-tocopherol. The extract exhibited inhibitory capacity against Fe3+/ascorbic acid induced phosphatidylcholine liposome oxidation, scavenged stable DPPH•, ABTS•+and superoxide anion radicals, and acted as reductant. In contrast, the leaf inner gel did not show any antioxidant activity. It was concluded that the known beneficial effects ofAloe veracould be attributed to its antioxidant activity and could be related to the presence of phenolic compounds and antioxidant vitamins.

scholarly journals Protective effects of hesperetin on the quality of sperm, apoptosis, lipid peroxidation, and oxidative stress during the process of cryopreservation: An experimental study

2021 ◽  
Author(s):  
Jamal Valipour ◽  
Sina Mojaverrostami ◽  
Beheshteh Abouhamzeh ◽  
Masoumeh Abdollahi
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Experimental Study ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Control Group ◽  
Oxygen Species ◽  
Protective Effects ◽  
Beneficial Effects

Background: Hesperetin is a bioflavonoid compound, largely used in Chinese traditional medicine and found plenty in citrus fruits. Hesperetin has beneficial effects against different diseases. The sperm cryopreservation process is a common method that is used in infertility laboratories. It has been reported that during the cryopreservation process, the quality of sperm is significantly reduced. Objective: To investigate the effect of hesperetin on the quality of human spermatozoa during the cryopreservation process. Materials and Methods: In this experimental study, 22 sperm sample of normozoospermia men who referred to the infertility department of the Shariati Hospital (Tehran, Iran) Between October and November 2019 were collect and divided into three groups as: 1) fresh, 2) control (frozen-thawed group without treatment), and 3) treatment group as frozen-thawed samples supplemented with 20 μM hesperetin. Motility, Viability, morphology, Apoptotic-like changes, intracellular H2O2, intracellular O2−, and lipid peroxidation (LPO) was measured. Results: Hesperetin treatment during the cryopreservation process of human sperm significantly improved the viability, motility, and morphology rates of the spermatozoa after frozen-thawed process in control group (p < 0.01). In addition, it significantly reduced the reactive oxygen species (ROS) level, LPO level and increased the percentage of viable sperm cells with intact plasma membrane (p < 0.01) after frozen-thawed process. Conclusion: Hesperetin can improve the quality of human sperm and also protect human sperm against reactive oxygen species, LPO, and apoptosis during the cryopreservation-thawing process. Key words: Cryopreservation, Hesperetin, Spermatozoa, Reactive oxygen species.

scholarly journals Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mehdi Evazalipour ◽  
Pouya Safarzadeh Kozani ◽  
Pooria Safarzadeh Kozani ◽  
Sahar Shabani ◽  
Bahar Rezaei Soufi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Cell Line ◽  
Cellular Senescence ◽  
Fibroblast Cell ◽  
Protective Effects ◽  
Proliferating Cells ◽  
Beneficial Effects ◽  
Nih 3T3 ◽  
Embryonic Fibroblast

Background: Stress-induced cellular senescence is a perpetual state of cell cycle arrest occurring in proliferating cells in response to stressful conditions. It is believed that oxidative stress plays a unique role in this process. As a reactive chemical compound that can induce oxidative stress, acrylamide is widely applied in several fields. Carvacrol is a liquid phenolic monoterpenoid found in essential oils of some plants and is known for its antioxidant and anticarcinogenic properties. Objectives: The current study aimed to evaluate the effects of carvacrol on oxidative stress and cellular senescence induced by acrylamide in the NIH 3T3 cell line. Methods: NIH 3T3 embryonic fibroblast cells were exposed to different concentrations of acrylamide, carvacrol, and H2O2 in a cell culture medium. The level of β-galactosidase (SA-β-gal) activity, as a marker of cellular senescence, was measured using staining and quantitative assays. Furthermore, to measure oxidative stress parameters, the content of glutathione and lipid peroxidation were determined. Results: Acrylamide could induce premature senescence evident by the elevated lipid peroxidation and SA-β-gal activity and declined cell viability and glutathione. Moreover, carvacrol showed beneficial effects on both acrylamide- and H2O2-induced cellular senescence by significantly reversing or subsiding the effect of oxidative stress and mediating its consequences. Conclusions: It can be concluded that carvacrol has protective effects against oxidative cellular senescence induced by acrylamide in the NIH 3T3 cell line.

Litopenaeus vannamei muscle carotenoids versus astaxanthin: A comparison of antioxidant activity and in vitro protective effects against lipid peroxidation

2015 ◽  
Vol 9 ◽  
pp. 12-19 ◽  
Author(s):  
Fabiana Ourique da Silva ◽  
Vera L.C.G. Tramonte ◽  
Jane Parisenti ◽  
Juliana F. Lima-Garcia ◽  
Marcelo Maraschin ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Litopenaeus Vannamei ◽  
Protective Effects

scholarly journals Lycium europaeum Extract: A New Potential Antioxidant Source against Cisplatin-Induced Liver and Kidney Injuries in Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Ilhem Rjeibi ◽  
Anouar Feriani ◽  
Anouar Ben Saad ◽  
Jazia Sdayria ◽  
Issam Saidi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Animal Studies ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Protective Effects ◽  
Dpph Radical ◽  
Phytochemical Composition ◽  
Liver And Kidney

This study was designed to assess the protective effects of Lycium europaeum methanol extract (LEM) on liver and kidney injuries induced by cisplatin. The phytochemical composition, the antioxidant activity, and hepatorenal injury biomarkers were investigated. Results revealed that LEM exhibited a significant antioxidant activity in vitro on DPPH radical and H2O2 scavenging assays. In the animal studies, treatment with LEM significantly reduced the effects of cisplatin intoxication on serum liver biomarkers and serum renal biomarkers. Meanwhile, LEM diminishes significantly the effect of cisplatin on the level of lipid peroxidation in liver and kidney tissues. The activities of the antioxidant enzymes (reduced glutathione, glutathione peroxidase, superoxide dismutase, and catalase) were increased in groups pretreated with LEM and quercetin. Additionally, the normal histological structures of the liver and kidney were restored after treatment with LEM. This work clearly demonstrated that L. europaeum may be useful as a drug with hepato-nephroprotective potentials.

scholarly journals Citrus sudachi Peel Extract Suppresses Cell Proliferation and Promotes the Differentiation of Keratinocytes through Inhibition of the EGFR–ERK Signaling Pathway

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1468
Author(s):  
Shogo Abe ◽  
Misako Ueno ◽  
Mami Nishitani ◽  
Tetsuya Akamatsu ◽  
Takumi Sato ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Egf Receptor ◽  
Skin Diseases ◽  
Receptor Activation ◽  
Inhibitory Effect ◽  
Keratinocyte Differentiation ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Beneficial Effects ◽  
Keratinocyte Cell Line Hacat

Citrus sudachi is a well-known fruit in Tokushima Prefecture, Japan, and its peels are rich in phytochemicals, including phenolic compounds. Although it is expected that the extract of the C. sudachi peel elicits various beneficial physiological activities, the effect on the skin has not been investigated. In this study, we report that the aqueous extract from the peel of C. sudachi suppresses cell proliferation of the immortalized human keratinocyte cell line, HaCaT, and primary normal human epidermal keratinocytes. The extract of C. sudachi peel suppressed epidermal growth factor (EGF)-induced EGF receptor activation and tumor necrosis factor (TNF)-α-induced extracellular regulated kinase (ERK) 1/2 activation, which suggests that the extract exerts its inhibitory effect through inhibition of both the EGF receptor (EGFR) and its downstream molecules. Additionally, the extract of C. sudachi peel potentiated calcium-induced keratinocyte differentiation. These results suggest that the extract of C. sudachi peel may have beneficial effects against skin diseases that are characterized by hyperproliferation of epidermal keratinocytes, such as those seen in psoriasis and in cutaneous squamous cell carcinoma.

Antioxidant Activity of Resveratrol Compared with Common Food Additives

2001 ◽  
Vol 64 (3) ◽  
pp. 379-384 ◽  
Author(s):  
M. ANTONIA MURCIA ◽  
MAGDALENA MARTÍNEZ-TOMÉ
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Sodium Tripolyphosphate ◽  
Food Additives ◽  
The Other ◽  
Butylated Hydroxytoluene ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Scavenger Activity ◽  
Inhibit Lipid Peroxidation

Resveratrol is a phenolic compound of the stilbene family present in wines and various parts of the grape, including the skin. In this study, the antioxidant and prooxidant activities of resveratrol were compared with other antioxidants (butylated hydroxytoluene [BHT], butylated hydroxyacetone [BHA], phenol, propyl gallate [PG], sodium tripolyphosphate [TPP], α-tocopherol, and vanillin) widely used in foods. The ability of these compounds to inhibit lipid peroxidation was as follows: BHA &gt; resveratrol &gt; PG &gt; tripolyphosphate &gt; vanillin &gt; phenol &gt; BHT &gt; α-tocopherol, the first three inhibiting the peroxidation in a concentration-dependent manner. The order of OH scavenger activity of the tested compounds was BHA &gt; TPP &gt; BHT. Resveratrol and vanillin produced between 10 to 7% and 16 to 10% inhibition of the deoxyribose attack, respectively, but they do not scavenge OH▪. Neither the resveratrol analyzed nor PG or the rest of compounds reacted with H2O2 and must be considered inefficient in catalyzing any subsequent oxidation. The ability to scavenge HOCl was, in decreasing order, PG &gt; resveratrol &gt; α-tocopherol &gt; phenol. The other compounds did not scavenge HOCl.

scholarly journals In Vitro antioxidant activity of Polygonum Glabrum 

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Raja Sundara rajan ◽  
Ramya I
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Superoxide Anion ◽  
Methanol Extract ◽  
Butylated Hydroxytoluene ◽  
In Vitro Antioxidant Activity

<p>Assessment of antioxidant activity was imperative in the screening of medicinal plants for potential health benefits. In present study methanol extract of <em>Polygonum glabrum</em> <em> </em>(polygonaceae) was screened for its in vitro antioxidant activity using biologically relevant methodologies which scavenge radicals such as  1,1 diphenyl 2 picryl hydrazyl (DPPH), nitric oxide, hydrogen peroxide, hydroxyl,  superoxide anion and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Total reducing ability by conversion of ferric (III) to ferrous (II) and molybdenum (VI) to molybdenum (V), metal ion chelating capacity and anti lipid peroxidation activities were also examined. The antioxidant ability of <em>Polygonum glabrum</em> whole plant extract was found to be in a dose dependent manner. The IC<sub>50</sub> values for scavenging of DPPH<sup>●</sup> and ABTS<sup>●+</sup> free radicals were 13.18 μg/ml and 20.46 μg/ml. For scavenging of nitric oxide, hydrogen peroxide, hydroxyl and superoxide anion radicals, the IC<sub>50</sub> values were found to be 80.22 μg/ml, 33.06 μg/ml, 52.26μg/ml and 36.98 μg/ml respectively. Further, addition of 120μg/ml of plant extract to the reaction mixture produced 50% lipid peroxidation inhibition activity. Commercial antioxidants such as vitamin E, quercetin, butylated hydroxytoluene and ascorbic acid were used as reference compounds. The strong antioxidant activity of <em>Polygonum glabrum</em> may be credited to the presence of triterpenes [beta-hydroxyfriedalanol], phenols        [3-hydroxy-5-methoxystilbene], flavonoids [pinocembrin and pinocembrin-5-methylether], steroids [sitosterol - (6-O-palmitoyl)- 3-O-β-D glucopyranoside and sitosterol-3-O-β-D glucopyranoside], sesqueterpenes [2,3-dihydroxy isodrimeninol] and pigments etc in methanol extract.</p>

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