Flavonol Glycosides from Distilled Petals of Rosa damascena Mill

Flavonol glycosides were extracted from petals of Rosa damascena Mill. after industrial distillation for essential oil recovery and characterized by high-performance liquid chromatography-electrospray ionization mass spectrometry. Among the 22 major compounds analyzed, only kaempferol and quercetin glycosides were detected. To the best of our knowledge, the presence of quercetin 3-O-galactoside and quercetin 3-O-xyloside has so far not been reported within the genus Rosa. In addition, based on their fragmentation patterns, several acylated quercetin and kaempferol glycosides, some of them being disaccharides, were identified for the first time. The kaempferol glycosides, along with the kaempferol aglycone, accounted for 80% of the total compounds that were quantified, with kaempferol 3-O-glucoside being the predominant component. The high flavonol content of approximately 16 g/kg on a dry weight basis revealed that distilled rose petals represent a promising source of phenolic compounds which might be used as functional food ingredients, as natural antioxidants or as color enhancers.

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ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of Chaenomeles Fruits Determined by Polyphenols and other Chemical Compounds

Antioxidants ◽

10.3390/antiox9010060 ◽

2020 ◽

Vol 9 (1) ◽

pp. 60 ◽

Cited By ~ 2

Author(s):

Igor Piotr Turkiewicz ◽

Aneta Wojdyło ◽

Karolina Tkacz ◽

Paulina Nowicka ◽

Tomasz Golis ◽

...

Keyword(s):

Pancreatic Lipase ◽

Biological Activities ◽

Dry Weight ◽

Array Detector ◽

Ionization Mass ◽

Photodiode Array Detector ◽

On Line ◽

Polyphenolic Profile ◽

Promising Source

This study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of Chaenomeles × superba, Chaenomeles japonica, and Chaenomeles speciosa by liquid chromatography coupled with photodiode array detector and quadrupole time-of-flight electrospray ionization mass spectrometry (LC-PDA-QTOF-ESI-MS). Antioxidant (ABTS on-line, ABTS, FRAP, and ORAC), as well as in vitro biological activities, i.e., the ability to inhibit α-amylase, α-glucosidase, pancreatic lipase, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and 15-lipoxygenase (15-LOX) were determined. Most of the Chaenomeles species and cultivars analyzed in this study have not been examined in this respect until now. Fruits contained 30.26 to 195.05 mg of vitamin C, 0.65 to 1.69 g of pectin, 0.32 to 0.64 g of ash, 0.60 to 3.98 g of sugars, and 41.64 to 110.31 g of organic acids in 100 g fresh weight. The lowest content of total polyphenols showed C. speciosa ‘Rubra’ (57.84 g/kg dry weight, dw) while C. × superba ’Nicoline’ (170.38 g/kg dw) exhibited the highest concentration of those compounds. In the phenolic compounds, polymeric procyanidin fraction predominated (65%) with procyanidin B2, C1, and (−)-epicatechin the most abundant. The antioxidant capacity measured by ABTS assay was mainly formed by polymeric procyanidins and flavan-3-ols, which was confirmed by ABTS on-line profiling. Chaenomeles fruits showed high potential for inhibition of α-glucosidase and pancreatic lipase. The analyzed cultivars displayed greater potential for acetylcholinesterase (AChE) inhibition than for butyrylcholinesterase (BuChE). The data indicate that Chaenomeles fruits could be regarded as a promising source of bioactive functional food.

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Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS

International Journal of Analytical Chemistry ◽

10.1155/2009/530639 ◽

2009 ◽

Vol 2009 ◽

pp. 1-9 ◽

Cited By ~ 20

Author(s):

Chiara Borromei ◽

Maria Careri ◽

Antonella Cavazza ◽

Claudio Corradini ◽

Lisa Elviri ◽

...

Keyword(s):

High Performance ◽

Anion Exchange Chromatography ◽

Exchange Chromatography ◽

Ionization Mass ◽

Good Resolution ◽

Food Ingredients ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Pulsed Electrochemical Detection ◽

Tof Ms

This paper describes the complementarity of high-performance anion exchange chromatography coupled with pulsed electrochemical detection (HPAEC-PED) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) to evaluate commercial available fructans (fructooligosaccharides (FOS) and inulins), having different degrees of polymerization (DP) which are usually employed by food industry as functional ingredients either for their prebiotic properties or as a fat replacer, giving a fat-like mouth feel and texture. The developed HPAEC-PED methods are able to analyze FOS (fructans with DP 3–10) and inulins (DP ranging from 3 to 80) with a good resolution and relatively short retention times to evaluate structural differences between fructooligosaccharide and inulins and the possible presence of inulooligosaccharides as well as of branching. To characterize FOS and inulin at different degrees of polymerization and to assure correct molecular assignment, MALDI-TOF MS analysis was also investigated. The 2,5-dihydroxy benzoic acid (2,5-DHB) was found to be the best matrix for FOS analysis as Actilight and Raftilose P95 products, while 3-aminoquinoline (3-AQ) seems to be the best matrix for inulin with higher DP. The applicability of the optimized methods to the identification and determination of FOS contained in a symbiotic milk as well as a type of inulin added as functional ingredient to a cooked ham is demonstrated.

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Biotransformation of quercetin, kaempferol and apigenin to monoglycosylated derivatives by in vitro suspension cultures of Astragalus vesicarius ssp. carniolicus

Pharmacia ◽

10.3897/pharmacia.68.e54289 ◽

2021 ◽

Vol 68 (2) ◽

pp. 307-311

Author(s):

Pavlinka Popova ◽

Yancho Zarev ◽

Iliana Ionkova

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Resolution ◽

Electrospray Ionization ◽

High Performance ◽

Dry Weight ◽

Suspension Cultures ◽

Ionization Mass

Biotransformation of exogenous substrates quercetin, kaempferol and apigenin by suspension cultures of Astragalus vesicarius ssp. carniolicus to their monoglycosylated derivatives was performed. The maximal enzymatic potential of cells of A. vesicarius ssp. carniolicus was evaluated by different concentrations of substrate exposure. According to quantitative ultra-high performance liquid chromatography-high resolution electrospray ionization mass spectrometry (UHPLC-HR-ESI-MS) analysis, the highest concentration of kaempferol O-glycoside (14.88 nmol/g dry weight, DW), apigenin O-glycoside (10.55 nmol/g DW) and quercetin O-glycoside (150.83 nmol/g DW) was achieved, when suspension cultures were treated with 4 mg/mL kaempferol, 4 mg/mL apigenin and 3 mg/mL quercetin, respectively. The glycosidic products of biotransformation were not detected in the untreated control.

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Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.03722-14 ◽

2015 ◽

Vol 59 (4) ◽

pp. 2169-2178 ◽

Cited By ~ 19

Author(s):

A. Srivastava ◽

B. N. Singh ◽

D. Deepak ◽

A. K. S. Rawat ◽

B. R. Singh

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Quorum Sensing ◽

High Performance ◽

Signal Molecules ◽

Antibiofilm Activity ◽

Ionization Mass ◽

Strong Inhibitor ◽

Content Type ◽

Promising Source

ABSTRACTThe discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling inStaphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD),1H and13C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS againstS. aureusand its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance inS. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.

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Characterization of Vitamin B12 Compounds in Fermented Poultry Manure Fertilizers

Agriculture ◽

10.3390/agriculture11070627 ◽

2021 ◽

Vol 11 (7) ◽

pp. 627

Author(s):

Hiromi Katsura ◽

Kyohei Koseki ◽

Tomohiro Bito ◽

Shigeo Takenaka ◽

Fumio Watanabe

Keyword(s):

Liquid Chromatography ◽

Vitamin B12 ◽

High Performance ◽

Organic Fertilizer ◽

Poultry Manure ◽

Dry Weight ◽

Ionization Mass ◽

Rf Values ◽

Pseudovitamin B12

(1) Background: Currently, no data are available on the vitamin B12 content of an organic fertilizer product, viz. fermented poultry manure, or whether the organic fertilizer product contains vitamin B12 or inactive corrinoids (or both). (2) Methods: This study conducted a microbiological assay to determine the vitamin B12 content of various commercially available fermented poultry manure fertilizer products. (3) Results: The results varied from 1.4 μg to approximately 20 μg per 100 g of dry weight. In the bioautography analysis, selected products had two positive spots with identical Rf values of vitamin B12 and pseudovitamin B12. High-performance liquid chromatography and liquid chromatography/electrospray ionization−mass spectrometry analyses of the selected products indicated that these fertilizers primarily contained vitamin B12. They also contained minor inactive cobamides such as pseudovitamin B12, 2-methyladenyl cobamide, and 2-methylmercaptoadenyl cobamide. (4) Conclusions: These results suggested that edible plants would enrich vitamin B12 using fermented poultry manure organic fertilizer products.

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Antiangiogenic and Antioxidant In Vitro Properties of Hydroethanolic Extract from açaí (Euterpe oleracea) Dietary Powder Supplement

Molecules ◽

10.3390/molecules26072011 ◽

2021 ◽

Vol 26 (7) ◽

pp. 2011

Author(s):

Raquel Costa ◽

Daniela Azevedo ◽

Pedro Barata ◽

Raquel Soares ◽

Luís F. Guido ◽

...

Keyword(s):

High Performance ◽

Antioxidant Defenses ◽

Migration And Invasion ◽

Ionization Mass ◽

Euterpe Oleracea ◽

Oxidative Markers ◽

Vessel Growth ◽

Promising Source ◽

Hydroethanolic Extract

The Euterpe oleracea fruit (açaí) is a promising source of polyphenols with health-promoting properties. To our knowledge, few studies have focused on the influence of açaí phytochemicals on angiogenesis, with a significant impact on cancer. This study aimed at investigating the phytochemical profile of a purple açaí hydroethanolic extract (AHE) obtained from a commercial dietary powder supplement by high-performance liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry, and evaluate its in vitro effects on distinct angiogenic steps during vessel growth and on oxidative markers in human microvascular endothelial cells (HMEC-1). The phenolic profile of AHE revealed the presence of significant levels of anthocyanins, mainly cyanidin-3-O-rutinoside, and other flavonoids with promising health effects. The in vitro studies demonstrated that AHE exerts antiangiogenic activity with no cytotoxic effect. The AHE was able to decrease HMEC-1 migration and invasion potential, as well as to inhibit the formation of capillary-like structures. Additionally, AHE increased antioxidant defenses by upregulating superoxide dismutase and catalase enzymatic activities, accompanied by a reduction in the production of reactive oxygen species. These data bring new insights into the potential application of angiogenic inhibitors present in AHE on the development of novel therapeutic approaches for angiogenesis-dependent diseases.

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Identification and Quantification of β-Sitosterol β-d-Glucoside of an Ethanolic Extract Obtained by Microwave-Assisted Extraction from Agave angustifolia Haw

Molecules ◽

10.3390/molecules24213926 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3926

Author(s):

Herminia López-Salazar ◽

Brenda Hildeliza Camacho-Díaz ◽

Sandra Victoria Ávila-Reyes ◽

Ma Dolores Pérez-García ◽

Manases González- Cortazar ◽

...

Keyword(s):

High Performance ◽

Ethanolic Extract ◽

Dry Weight ◽

Extraction Time ◽

Ionization Mass ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Agave Angustifolia ◽

Assisted Extraction ◽

Ft Ir

β-sitosterol β-d-glucoside (BSSG) was extracted from “piña” of the Agave angustifolia Haw plant by microwave-assisted extraction (MAE) with a KOH solution such as a catalyst and a conventional maceration method to determine the best technique in terms of yield, extraction time, and recovery. The quantification and characterization of BSSG were done by high-performance thin layer chromatography (HPTLC), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography−electrospray ionization−mass spectrometry (HPLC-ESI-MS). With an extraction time of 5 s by MAE, a higher amount of BSSG (124.76 mg of β-sitosterol β-d-glucoside/g dry weight of the extract) than those for MAE extraction times of 10 and 15 s (106.19 and 103.97 mg/g dry weight respectively) was shown. The quantification of BSSG in the extract obtained by 48 h of conventional maceration was about 4–5 times less (26.67 mg/g dry weight of the extract) than the yields reached by the MAE treatments. MAE achieved the highest amount of BSSG, in the shortest extraction time while preserving the integrity of the compound’s structure.

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Fucoxanthin and Phenolic Contents of Six Dictyotales From the Tunisian Coasts With an Emphasis for a Green Extraction Using a Supercritical CO2 Method

Frontiers in Marine Science ◽

10.3389/fmars.2021.647159 ◽

2021 ◽

Vol 8 ◽

Author(s):

Leila Ktari ◽

Cheima Mdallel ◽

Brahim Aoun ◽

Leila Chebil Ajjabi ◽

Saloua Sadok

Keyword(s):

High Performance ◽

Dry Weight ◽

Abundant Species ◽

Antioxidant Property ◽

Extraction Yield ◽

Added Value ◽

Bioactive Metabolites ◽

Total Phenol Content ◽

Dictyota Dichotoma ◽

Promising Source

Dictyotales, a common taxonomic group of brown seaweeds found in warm and temperate waters, are known for their richness in bioactive metabolites. In this study, six species of Dictyotales (Dictyopteris polypodioides, Dictyota dichotoma, Dictyota fasciola, Dictyota spiralis, Padina pavonica, and Taonia atomaria) collected from the Tunisian coasts were investigated for their antioxidant potentials, based on their contents of high added-value bioactive metabolites such as fucoxanthin and polyphenols. Fucoxanthin and polyphenols were analyzed quantitatively by high-performance liquid chromatography (HPLC) and UV spectrophotometer, respectively. The antioxidant property of extracts was also determined based on their ability to scavenge 2,2-Diphenyl-1-picrylhydrazyl (DPPH’s) free radical. Thus, the highest concentrations of fucoxanthin were obtained from T. atomaria and D. polypodioides (5.53 ± 1.2 and 3.43 ± 1.3 mg⋅g–1 dry weight, respectively), while the lowest amount was from D. spiralis (0.23 ± 0.1 m mg⋅g–1 dry weight). Dictyota dichotoma and T. atomaria gave the highest total phenol content (19.3 ± 0.4 and 15.2 ± 1.1 mg GAE⋅g–1 dry weight, respectively). In the second step, supercritical carbon dioxide (ScCO2) was used as a green and environmentally safe technique for the extraction of fucoxanthin from the most abundant species D. polypodioides. The extraction of fucoxanthin by ScCO2 allowed an extraction yield ranging from 0.50 ± 0.04% to 1.32 ± 0.02%, with 60°C temperature and 50-MPa pressure as the best extraction conditions. The maximum fucoxanthin and polyphenol recovery in the extract attained 15 and 64%, respectively. The results strengthen the possible use of Dictyotales from the Mediterranean Sea as a promising source of natural ingredients of health and economic interests contributing to Blue Growth in the region.

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Fruit and berry raw materials is a promising source of food ingredients for flour confectionery.

Khleboproducty ◽

10.32462/0235-2508-2020-29-10-45-49 ◽

2020 ◽

Vol 29 (11) ◽

pp. 45-49

Author(s):

L.N. Fedyanina ◽

◽

E.S. Smertina ◽

V.A. Lyakh ◽

A.E. Elizarova ◽

...

Keyword(s):

Dietary Fiber ◽

Scientific Literature ◽

Consumer Demand ◽

Raw Materials ◽

Plant Materials ◽

Food Ingredients ◽

Promising Source

The article considers the problem of improving the range of confectionery from the standpoint of use plant materials of satisfaction by consumer demand in dieteticpreventive foods. The analysis of domestic and foreign scientific literature on promising directions of improving the range of dietetic-preventive confectionery is given. It is noted that in the recipes for flour confectionery introduced from non-traditional raw materials containing dietary fiber.

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