Effect of Some Flavonic Compounds and Ascorbic Acid on Lactoferrin Stimulation of Erythrocyte Glycolysis and Na+/K+-ATPase Activity

2008 ◽  
Vol 63 (9-10) ◽  
pp. 773-779 ◽  
Author(s):  
Ana Maneva ◽  
Borislava Taleva
Keyword(s):  
Ascorbic Acid ◽  
Atpase Activity ◽  
Specific Binding ◽  
Lactate Production ◽  
Inhibitory Effect ◽  
High Concentration ◽  
Transport Chain ◽  
Strong Inhibitory Effect ◽  
Lactate Formation ◽  
Stimulation Of

The aim of the present study was to assess if some flavonic compounds (quercetin, piceatannol and apigenin) and ascorbic acid could interfere with the Lf stimulatory effect on the erythrocyte function. Quercetin (1.5 μm) and piceatannol (30 μm) showed an additive effect on Lf stimulation of Na+/K+-ATPase when used together with Lf. The enhancement of Lf stimulation on Na+/K+-ATPase in the presence of flavonoids was probably due to their antioxidative properties and/or to their involvement in the erythrocyte signaling. None of the estimated flavonoids showed an effect on Lf stimulation of the lactate production. Quercetin itself enhanced the ATPase activity but did not affect the lactate formation. Apigenin (1.5 μm) enhanced reliably the lactate generation, but it did not exert any effect on the ATPase activity. High concentration of ascorbic acid (60 mm) did not change the Lf stimulatory effect on Na+/K+-ATPase, but decreased the Lf-specific-binding. A significantly strong inhibitory effect on the Lf-specific binding exerted the electron acceptors NAD+ (2 mm) and FAD (2 mm). These effects concern most likely the competition with Lf for electron(s) which is (are) provided from the erythrocyte intercellular electron transport chain(s).

Abstract 341: Synergistic Effects of Renal Dopamine and Gastrin Receptors in Hypertension

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Yue Chen ◽  
Shuo Zhen ◽  
Laureano Asico ◽  
Pedro Jose ◽  
Chunyu Zeng
Keyword(s):  
Atpase Activity ◽  
Sodium Excretion ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
Receptor Interaction ◽  
Gastrin Receptor ◽  
Wky Rats ◽  
Link Type ◽  
Renal Dopamine ◽  
Stimulation Of

Oral NaCl produces stronger natriuresis and diuresis as compared with venous infusion of same amount of NaCl, indicating the existence of renal-gastric axis. Although numerous hormones are secreted in gastrointestinal tract, gastrin is evident one due to its natriuretic effects and taken-up by the renal proximal tubule (RPT) cells. We hypothesize that there is an interaction between gastrin and dopamine receptor in kidney, which synergistically increases sodium excretion, the impaired interaction would be involved in the pathogenesis of hypertension. In WKY rats, infusion of gastrin, via renal artery, induced natriuresis and diuresis, which was blocked in the presence of CI988, a gastrin receptor blocker. Similarly, the natriuretic and diuretic effect of fenoldopam, a D1-like receptor agonist, was blocked by the D1-like receptor antagonist, SCH23390 , indicating that gastrin and fenoldopam, via individual receptor, play natriuretic and diuretic effects. Our further study found that lower dosages of gastrin or fenoldopam could not induce natriuresis and diuresis alone, while putting together induced natriuretic and diuretic effects. The above-mentioned effects were lost in SHRs. We also found, in the presence of SCH23390 , gastrin-mediated natriuresis and diuresis was partially blocked. Similarly, in the presence of CI988, the natriuretic and diuretic effects of fenoldopam were partially blocked, indicating the interaction between gastrin and D1-like receptor. The gastrin/D1-like receptor interaction was also confirmed in the RPT cells. Stimulation of one receptor increased the expression of the other. Stimulation of either D1-like receptor or gastrin receptor inhibited the Na + -K + -ATPase activity in RPT cells, while in the presence of SCH23390 , the inhibitory effect of gastrin on Na + -K + -ATPase activity was partially blocked. In the presence of CI988, D1-like receptor-mediated inhibitory effect of Na + -K + -ATPase activity in RPT cells was partially inhibited. It indicated the synergistic effect between gastrin and D1-like receptor would increase the sodium excretion in WKY rats; the impaired interaction might be involved in the pathogenesis of hypertension.

scholarly journals Sodium iron EDTA and ascorbic acid, but not polyphenol oxidase treatment, counteract the strong inhibitory effect of polyphenols from brown sorghum on the absorption of fortification iron in young women

2013 ◽  
Vol 111 (3) ◽  
pp. 481-489 ◽  
Author(s):  
Colin I. Cercamondi ◽  
Ines M. Egli ◽  
Christophe Zeder ◽  
Richard F. Hurrell
Keyword(s):  
Ascorbic Acid ◽  
Polyphenol Oxidase ◽  
Young Women ◽  
Inhibitory Effect ◽  
Inhibitory Effects ◽  
Fe Isotopes ◽  
Absorption Studies ◽  
Oxidase Enzyme ◽  
Strong Inhibitory Effect

In addition to phytate, polyphenols (PP) might contribute to low Fe bioavailability from sorghum-based foods. To investigate the inhibitory effects of sorghum PP on Fe absorption and the potential enhancing effects of ascorbic acid (AA), NaFeEDTA and the PP oxidase enzyme laccase, we carried out three Fe absorption studies in fifty young women consuming dephytinised Fe-fortified test meals based on white and brown sorghum varieties with different PP concentrations. Fe absorption was measured as the incorporation of stable Fe isotopes into erythrocytes. In study 1, Fe absorption from meals with 17 mg PP (8·5 %) was higher than that from meals with 73 mg PP (3·2 %) and 167 mg PP (2·7 %;P< 0·001). Fe absorption from meals containing 73 and 167 mg PP did not differ (P= 0·9). In study 2, Fe absorption from NaFeEDTA-fortified meals (167 mg PP) was higher than that from the same meals fortified with FeSO4(4·6v.2·7 %;P< 0·001), but still it was lower than that from FeSO4-fortified meals with 17 mg PP (10·7 %;P< 0·001). In study 3, laccase treatment decreased the levels of PP from 167 to 42 mg, but it did not improve absorption compared with that from meals with 167 mg PP (4·8v.4·6 %;P= 0·4), whereas adding AA increased absorption to 13·6 % (P< 0·001). These findings suggest that PP from brown sorghum contribute to low Fe bioavailability from sorghum foods and that AA and, to a lesser extent, NaFeEDTA, but not laccase, have the potential to overcome the inhibitory effect of PP and improve Fe absorption from sorghum foods.

scholarly journals ATP-dependent interaction of propranolol and local anaesthetic with sarcoplasmic reticulum. Stimulation of Ca2+ efflux

1988 ◽  
Vol 256 (3) ◽  
pp. 733-739 ◽  
Author(s):  
V Shoshan-Barmatz
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Atpase Activity ◽  
Local Anaesthetics ◽  
Inhibitory Effect ◽  
Phospholipid Bilayer ◽  
Drug Induced ◽  
Protein Factor ◽  
Dependent Inhibition ◽  
Drug Modification ◽  
Stimulation Of

Preincubation of sarcoplasmic reticulum (SR) with propranolol or tetracaine inhibits Ca2+ accumulation and stimulates ATPase activity by more than 2-fold. This effect is obtained only when the preincubation is carried out in the presence of ATP or other nucleoside triphosphates. The (ATP + drug)-induced inhibition of Ca2+ accumulation is pH-dependent, increasing as the pH rises above 7.5. The presence of micromolar concentrations of Ca2+ or Mg2+ during the preincubation prevents the inhibitory effect of ATP plus drug on Ca2+ accumulation or ATPase activity. The (ATP + drug) modification of SR vesicles resulted in stimulation of a rapid Ca2+ efflux from passively loaded vesicles. The ATP-dependent inhibition of Ca2+ accumulation by the drug is obtained with other local anaesthetics. The drug concentration required for 50% inhibition was 0.15 mM for dibucaine and 0.4 mM for both propranolol and tetracaine, whereas it was 5 mM, 8 mM and greater than 10 mM for lidocaine, benzocaine and procaine respectively. The heavy SR vesicles were only slightly affected by the incubation with propranolol or tetracaine in the presence of ATP, but their sensitivity increased markedly after storage at 0 degrees C for 24-48 h. These results suggest that propranolol and some local anaesthetics, in the presence of ATP, stimulate Ca2+ efflux by modifying a protein factor(s) rather than the phospholipid bilayer.

Interleukin-1 inhibition of Na(+)-K(+)-ATPase in inner medullary collecting duct cells: role of PGE2

1991 ◽  
Vol 261 (6) ◽  
pp. F1013-F1016 ◽  
Author(s):  
M. L. Zeidel ◽  
H. R. Brady ◽  
D. E. Kohan
Keyword(s):  
Atpase Activity ◽  
Collecting Duct ◽  
Interleukin 1 ◽  
Inhibitory Effect ◽  
Inner Medullary Collecting Duct ◽  
Duct Cells ◽  
Natriuretic Effect ◽  
Collecting Duct Cells ◽  
Medullary Collecting Duct ◽  
Stimulation Of

Interleukin-1 (IL-1), a cytokine produced by macrophages, causes an increase in Na+ excretion in experimental animals. Micropuncture studies have determined that the natriuretic effect of IL-1 is largely due to inhibition of Na+ reabsorption in the collecting duct. The current studies made use of suspensions of rabbit inner medullary collecting duct (IMCD) cells to examine the mechanism by which IL-1 regulates Na+ transport. IL-1 reduced ouabain-sensitive 86Rb+ uptake by 48% at 10 s, 36% at 30 s, and 29% at 60 s, suggesting an inhibitory effect on Na(+)-K(+)-adenosinetriphosphatase (ATPase) activity. IL-1 inhibition of 86Rb+ uptake occurred in a dose-dependent manner. This effect appears to be mediated by prostaglandin E2 (PGE2) because 1) ibuprofen blocks the inhibitory effect of IL-1 on IMCD Na(+)-K(+)-ATPase activity, 2) IL-1 and PGE2 cause equivalent and nonadditive inhibition of 86Rb+ uptake, 3) IL-1 causes a two- to threefold increase in PGE2 content in IMCD cells, and 4) dose-response curves were similar for IL-1 stimulation of PGE2 content and inhibition of 86Rb+ uptake in IMCD cells. Thus the natriuretic effect of IL-1 is due, at least in part, to stimulation of PGE2 production by collecting duct cells with resultant inhibition of Na(+)-K(+)-ATPase activity.

Mode of action of somatostatin to inhibit secretion by shark rectal gland

1985 ◽  
Vol 249 (3) ◽  
pp. R329-R334 ◽  
Author(s):  
P. Silva ◽  
J. S. Stoff ◽  
D. R. Leone ◽  
F. H. Epstein
Keyword(s):  
Cellular Response ◽  
Specific Binding ◽  
Phosphodiesterase Inhibitor ◽  
Inhibitory Effect ◽  
Squalus Acanthias ◽  
Rectal Gland ◽  
Gland Cells ◽  
Mechanism Of Inhibition ◽  
Rectal Glands ◽  
Stimulation Of

The rectal gland of the spiny dogfish Squalus acanthias is stimulated to secrete chloride by vasoactive intestinal peptide (VIP) in a way that is inhibited by somatostatin. The mechanism of inhibition by somatostatin was studied in isolated perfused rectal glands and separated rectal gland cells. Somatostatin did not alter the specific binding of VIP to rectal gland cells but inhibited their accumulation of adenosine 3',5'-cyclic monophosphate (cAMP) in response to VIP. In isolated perfused glands, somatostatin inhibited the stimulation of secretion produced by VIP, adenosine, and forskolin, as well as by dibutyryl cAMP plus a phosphodiesterase inhibitor. The results support the hypothesis of both a proximal and a distal locus, in the cascade of events leading from adenylate cyclase activation to cellular response, at which somatostatin exerts an inhibitory effect.

Suppression of the Cerebral Vasospastic Actions of Oxyhemoglobin by Ascorbic Acid

Neurosurgery ◽  
1991 ◽  
Vol 28 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Masahisa Kawakami ◽  
Namio Kodama ◽  
Noboru Toda
Keyword(s):  
Ascorbic Acid ◽  
Biological Activity ◽  
Subarachnoid Hemorrhage ◽  
Cerebral Arteries ◽  
Inhibitory Effect ◽  
Cisternal Irrigation ◽  
Dose Dependent Fashion ◽  
Anesthetized Dogs ◽  
Dose Dependent ◽  
Stimulation Of

Abstract Oxyhemoglobin (Oxy-Hb) produced a concentration-dependent contraction of monkey, dog, and bovine cerebral artery strips. Treatment of Oxy-Hb with ascorbic acid suppressed the ability of Oxy-Hb to contract the arteries, especially in the monkey arteries. The ability of intracisternally applied Oxy-Hb to constrict the basilar artery in anesthetized dogs was diminished when Oxy-Hb was treated previously with ascorbic acid (AsA-Hb). The contraction caused by Oxy-Hb was suppressed by treatment with indomethacin and aspirin in isolated bovine cerebral arteries. Endothelium-dependent relaxations elicited by substance P and relaxations induced by stimulation of the vasodilator nerves with nicotine were suppressed by treatment with Oxy-Hb and AsA-Hb; however, the inhibitory effect of AsA-Hb was markedly less. Oxy-Hb attenuated nitroglycerin-induced relaxations in a dose-dependent fashion, whereas AsA-Hb in concentrations up to 1.6 x 10-5 M did not significantly influence the relaxations. It is concluded that incubation of Oxy-Hb with ascorbic acid alters markedly the biological activity of Oxy-Hb; the vasoconstrictor activity is suppressed, and the ability to diminish vasodilator actions is minimized. These findings provide a rationale for the use of ascorbic acid in cisternal irrigation to prevent the development of cerebral vasospasm after a subarachnoid hemorrhage.

Exogenous ascorbic acid or thiamine increases the resistance of sunflower and maize plants to salt stress

2009 ◽  
Vol 57 (3) ◽  
pp. 335-347 ◽  
Author(s):  
A. Hamada ◽  
A. Al-Hakimi
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Net Photosynthetic Rate ◽  
Photosynthetic Rate ◽  
Membrane Integrity ◽  
Inhibitory Effect ◽  
Favourable Effect ◽  
High Concentration ◽  
Stimulatory Action ◽  
Salinity Levels

Increasing NaCl levels retarded the net photosynthetic rate, biosynthesis of photosynthetic pigments and membrane integrity of maize and sunflower seedlings; a serious effect was exhibited when NaCl was applied at high concentration. On the other hand, the K + efflux increased at increasing NaCl levels. In addition, the various salt levels induced considerable variations in the concentrations of sodium, potassium, calcium and magnesium. The vitamins applied were generally effective in partially or completely countering the inhibitory effects of salt stress on net photosynthetic rate, pigments biosynthesis and membrane integrity, exerting a stimulatory action on these parameters, especially in plants subjected to moderate and low salinity levels. The leakage of K + was reduced by the application of both ascorbic acid (AsA) and thiamine (B 1 ). Soaking the seeds of salt-stressed plants in AsA or B 1 had a favourable effect on the accumulation of certain ions and antagonized or ameliorated the inhibitory effect of salt stress.

Reexamination of dopamine as the prolactin-release inhibiting factor (PIF): supplementary agent may be required for dopamine to function as the physiological PIF

1990 ◽  
Vol 68 (9) ◽  
pp. 1226-1230 ◽  
Author(s):  
Seon H. Shin ◽  
Samia F. Hanna ◽  
Murray Hong ◽  
Khem Jhamandas
Keyword(s):  
Ascorbic Acid ◽  
Culture System ◽  
Monolayer Culture ◽  
Petri Dish ◽  
Inhibitory Effect ◽  
Male Rat ◽  
High Concentration ◽  
Prolactin Release ◽  
Inhibiting Factor ◽  
Perifusion System

A large number of studies have been performed concerning dopamine's inhibitory effect on prolactin release, but many of these studies have examined the effect of dopamine dissolved in a solution containing ascorbic acid. Ascorbic acid, routinely used to protect dopamine from oxidation, alone does not stimulate or inhibit prolactin release, but it can potentiate the inhibitory effect of dopamine in a static monolayer culture system by approximately 100 times. We have closely examined the inhibitory effect of dopamine on prolactin release in the absence of ascorbic acid using a perifusion system. Male rat adenohypophyses were dispersed with trypsin and cultured in a Petri dish to form cell clusters. Inhibition of prolactin release by dopamine (1 μmol/L) in the absence of ascorbic acid was sustained for only 63 min during the 2-h perifusion period. Following a 2-h period of incubation of dopamine in the same experimental solution, the dopamine concentration was reduced from 1 to 0.18 μmol/L, yet this "2-h-old dopamine" was still effective in inhibiting prolactin release (approximately 30 min). This result suggests that the lactotrophs may be desensitized by chronic exposure to a high concentration of dopamine in the absence of ascorbic acid. In contrast, when a low concentration of dopamine (3 nmol/L) containing ascorbic acid (0.1 mmol/L) was perifused, inhibition of prolactin release was sustained for the entire 2-h perifusion period. Although there may be a large number of explanations for dopamine's transient inhibitory effect on prolactin release, the present results suggest that dopamine may require supplementary agent(s) to effectively inhibit prolactin release and thus function as the prolactin release inhibitory factor (PIF). We propose ascorbic acid as a major candidate for the supplementary factor for the PIF.Key words: dopamine, somatostatin, prolactin, cell cluster, perifusion.

scholarly journals Stimulation of both aerobic glycolysis and Na+-K+-ATPase activity in skeletal muscle by epinephrine or amylin

1999 ◽  
Vol 277 (1) ◽  
pp. E176-E186 ◽  
Author(s):  
J. Howard James ◽  
Kenneth R. Wagner ◽  
Jy-Kung King ◽  
Rebecca E. Leffler ◽  
Radha Krishna Upputuri ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Atpase Activity ◽  
Extensor Digitorum Longus ◽  
Aerobic Glycolysis ◽  
Lactate Production ◽  
Extensor Digitorum ◽  
Atp Production ◽  
High Concentrations ◽  
First Time ◽  
Stimulation Of

Epinephrine and amylin stimulate glycogenolysis, glycolysis, and Na+-K+-ATPase activity in skeletal muscle. However, it is not known whether these hormones stimulate glycolytic ATP production that is specifically coupled to ATP consumption by the Na+-K+pump. These studies correlated glycolysis with Na+-K+-ATPase activity in resting rat extensor digitorum longus and soleus muscles incubated at 30°C in well-oxygenated medium. Lactate production rose three- to fourfold, and the intracellular Na+-to-K+ratio (Na+/K+) fell with increasing concentrations of epinephrine or amylin. In muscles exposed to epinephrine at high concentrations (5 × 10−7 and 5 × 10−6 M), ouabain significantly inhibited glycolysis by ∼70% in either muscle and inhibited glycogenolysis by ∼40 and ∼75% in extensor digitorum longus and soleus, respectively. In the absence of ouabain, but not in its presence, statistically significant inverse correlations were observed between lactate production and intracellular Na+/K+for each hormone. Epinephrine had no significant effect on oxygen consumption or ATP content in either muscle. These results suggest for the first time that stimulation of glycolysis and glycogenolysis in resting skeletal muscle by epinephrine or amylin is closely linked to stimulation of active Na+-K+transport.

scholarly journals Chemical and Bioactivity Evaluation of Eryngium planum and Cnicus benedictus Polyphenolic-Rich Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Gabriela Paun ◽  
Elena Neagu ◽  
Veronica Moroeanu ◽  
Camelia Albu ◽  
Simona Savin ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Chlorogenic Acid ◽  
Ursolic Acid ◽  
Biological Activities ◽  
Sinapic Acid ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
High Concentration ◽  
Standard Compound ◽  
Inhibitory Activities

This study evaluated the biological activities of Eryngium planum and of Cnicus benedictus extracts enriched in polyphenols obtained by nanofiltration. The HPLC-MS analysis showed that E. planum contains mainly flavonoids, especially rutin, while in C. benedictus extracts show the high concentration of the phenolic acids, principally the chlorogenic acid and sinapic acid. Herein, there is the first report of ursolic acid, genistin, and isorhamnetin in E. planum and C. benedictus. C. benedictus polyphenolic-rich extract showed high scavenging activity (IC50=0.0081 mg/mL) comparable to that of standard compound (ascorbic acid) and a higher reducing power (IC50= 0.082 mg/mL), with IC50 having a significantly lower value than IC50 for ascorbic acid. Both extracts were nontoxic to NCTC cell line. Among the investigated herbs, E. planum polyphenolic-rich extract showed the highest inhibitory activities with the IC50 value of 31.3 μg/mL for lipoxygenase and 24.6 μg/mL for hyaluronidase. Both polyphenolic-rich extracts had a higher inhibitory effect on α-amylase and α-glucosidase than that of the acarbose. The synergistic effect of ursolic acid, rutin, chlorogenic acid, rosmarinic acid, genistin, and daidzein identified in polyphenolic-rich extracts could be mainly responsible for the pharmacological potentials of the studied extracts used in managing inflammation and diabetes.

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