scholarly journals Chemical Characterization and Physical and Biological Activities of Rhamnolipids Produced by Pseudomonas aeruginosa BN10

2011 ◽  
Vol 66 (7-8) ◽  
pp. 394-402
Author(s):  
Nelly Christova ◽  
Boryana Tuleva ◽  
Rashel Cohen ◽  
Galya Ivanova ◽  
Georgy Stoev ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Pure Water ◽  
Carbon Sources ◽  
Chemical Characterization ◽  
Critical Micellar Concentration ◽  
Antimicrobial Activities ◽  
Chemical Structures ◽  
Enhanced Production ◽  
Pharmaceutical Industries

Pseudomonas aeruginosa BN10 isolated from hydrocarbon-polluted soil was found to produce rhamnolipids when cultivated on 2% glycerol, glucose, n-hexadecane, and n-alkanes. The rhamnolipids were partially purified on silica gel columns and their chemical structures elucidated by combination of one- and two-dimensional 1H and 13C NMR techniques and ESI-MS analysis. Eight structural rhamnolipid homologues were identified: Rha-C10- C8, Rha-C10-C10, Rha-C10-C12:1, Rha-C10-C12, Rha2-C10-C8, Rha2-C10-C10, Rha2-C10-C12:1, and Rha2-C10-C12. The chemical composition of the rhamnolipid mixtures produced on different carbon sources did not vary with the type of carbon source used. The rhamnolipid mixture produced by Pseudomonas aeruginosa BN10 on glycerol reduced the surface tension of pure water from 72 to 29 mN m-1 at a critical micellar concentration of 40 mg l-1, and the interfacial tension was 0.9 mN m-1. The new surfactant product formed stable emulsions with hydrocarbons and showed high antimicrobial activity against Gram-positive bacteria. The present study shows that the new strain Pseudomonas aeruginosa BN10 demonstrates enhanced production of the di-rhamnolipid Rha2-C10-C10 on all carbon sources used. Due to its excellent surface and good antimicrobial activities the rhamnolipid homologue mixture from Pseudomonas aeruginosa BN10 can be exploited for use in bioremediation, petroleum and pharmaceutical industries.

scholarly journals Secondary Metabolites from Gorgonian Corals of the Genus Eunicella: Structural Characterizations, Biological Activities, and Synthetic Approaches

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 129
Author(s):  
Dario Matulja ◽  
Maria Kolympadi Markovic ◽  
Gabriela Ambrožić ◽  
Sylvain Laclef ◽  
Sandra Kraljević Pavelić ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Biological Activities ◽  
Structural Characteristics ◽  
Antimicrobial Activities ◽  
Chemical Structures ◽  
Gorgonian Corals ◽  
Synthetic Approaches ◽  
Further Development

Gorgonian corals, which belong to the genus Eunicella, are known as natural sources of diverse compounds with unique structural characteristics and interesting bioactivities both in vitro and in vivo. This review is focused primarily on the secondary metabolites isolated from various Eunicella species. The chemical structures of 64 compounds were divided into three main groups and comprehensively presented: a) terpenoids, b) sterols, and c) alkaloids and nucleosides. The observed biological activities of depicted metabolites with an impact on cytotoxic, anti-inflammatory, and antimicrobial activities were reviewed. The most promising biological activities of certain metabolites point to potential candidates for further development in pharmaceutical, cosmetic, and other industries, and are highlighted. Total synthesis or the synthetic approaches towards the desired skeletons or natural products are also summarized.

scholarly journals A Comprehensive Insight on the Health Benefits and Phytoconstituents of Camellia sinensis and Recent Approaches for Its Quality Control

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 455 ◽  
Author(s):  
Aboulwafa ◽  
Youssef ◽  
Gad ◽  
Altyar ◽  
Al-Azizi ◽  
...  
Keyword(s):  
Quality Control ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Biological Activities ◽  
Health Benefits ◽  
Antimicrobial Activities ◽  
The Family ◽  
Complex Chemical Composition ◽  
Anti Oxidant ◽  
Pharmaceutical Industries

Tea, Camellia sinensis, which belongs to the family Theaceae, is a shrub or evergreen tree up to 16 m in height. Green tea is very popular because of its marked health benefits comprising its anticancer, anti-oxidant, and antimicrobial activities, as well as its effectiveness in reducing body weight. Additionally, it was recognized by Chinese people as an effective traditional drink required for the prophylaxis against many health ailments. This is due to the complex chemical composition of green tea, which comprises different classes of chemical compounds, such as polyphenols, alkaloids, proteins, minerals, vitamins, amino acids, and others. The beneficial health effects of green tea ultimately led to its great consumption and increase its liability to be adulterated by either low-quality or non-green tea products with concomitant decrease in activity. Thus, in this review, green tea was selected to highlight its health benefits and phytoconstituents, as well as recent approaches for its quality-control monitoring that guarantee its incorporation in many pharmaceutical industries. More research is needed to find out other more biological activities, active constituents, and other simple and cheap techniques for its quality assurance that ascertain the prevention of its adulteration.

scholarly journals Microfactories-Bioproduction of Chemicals and Pharmaceuticals by using Microbes

2021 ◽  
pp. 44-53
Author(s):  
Attiya Rasool
Keyword(s):  
Natural Products ◽  
Recombinant Dna ◽  
Biological Activities ◽  
Carbon Sources ◽  
Chemical Production ◽  
Recombinant Dna Technology ◽  
Fine Chemical ◽  
Chemical Structures ◽  
Microbial Cell Factories ◽  
Cell Factories

A variety of organisms, such as bacteria, fungi, and plants, produce secondary metabolites, also known as natural products. Natural products have been a prolific source and an inspiration for numerous medical agents with widely divergent chemical structures and biological activities, including antimicrobial, immunosuppressive, anticancer, and anti-inflammatory activities, many of which have been developed as treatments and have potential therapeutic applications for human diseases. Aside from natural products, the recent development of recombinant DNA technology has sparked the development of a wide array of biopharmaceutical products, such as recombinant proteins, offering significant advances in treating a broad spectrum of medical illnesses and conditions. Fine chemicals that are physiologically active, such as pharmaceuticals, cosmetics, nutritional supplements, flavoring agents as well as additives for foods, feed, and fertilizer are produced by enzymatically or through microbial fermentation. The identification of enzymes that catalyze the target reaction makes possible to synthesis of the desired fine chemical. The genes encoding these enzymes are then introduced into suitable microbial hosts that are cultured with inexpensive, naturally abundant carbon sources, and other nutrients. Metabolic engineering create efficient microbial cell factories for producing chemicals at higher yields. In the present review, we summarize recent studies on bio-based fine chemical production and assess the potential of synthetic bioengineering for further improvement their productivity.

Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste

2006 ◽  
Vol 97 (18) ◽  
pp. 2457-2461 ◽  
Author(s):  
Benjamas Thanomsub ◽  
Wanna Pumeechockchai ◽  
Anirut Limtrakul ◽  
Panarat Arunrattiyakorn ◽  
Wipawan Petchleelaha ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Chemical Structures

scholarly journals Isolation and Antibacterial Activity of Indole Alkaloids from Pseudomonas aeruginosa UWI-1

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3744
Author(s):  
Antonio Ramkissoon ◽  
Mohindra Seepersaud ◽  
Anderson Maxwell ◽  
Jayaraj Jayaraman ◽  
Adesh Ramsubhag
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
High Resolution Mass Spectrometry ◽  
Indole Alkaloid ◽  
Antimicrobial Activities ◽  
Mass Spectrometry Data ◽  
Gram Positive ◽  
Chemical Structures ◽  
Wide Range

In this study, we report the first isolation of three antibiotic indole alkaloid compounds from a Pseudomonad bacterium, Pseudomonas aeruginosa UWI-1. The bacterium was batch fermented in a modified Luria Broth medium and compounds were solvent extracted and isolated by bioassay-guided fractionation. The three compounds were identified as (1) tris(1H-indol-3-yl) methylium, (2) bis(indol-3-yl) phenylmethane, and (3) indolo (2, 1b) quinazoline-6, 12 dione. A combination of 1D and 2D NMR, high-resolution mass spectrometry data and comparison from related data from the literature was used to determine the chemical structures of the compounds. Compounds 1–3 were evaluated in vitro for their antimicrobial activities against a wide range of microorganisms using the broth microdilution technique. Compounds 1 and 2 displayed antibacterial activity against only Gram-positive pathogens, although 1 had significantly lower minimum inhibitory concentration (MIC) values than 2. Compound 3 displayed potent broad-spectrum antimicrobial activity against a range of Gram positive and negative bacteria. Several genes identified from the genome of P. aeruginosa UWI-1 were postulated to contribute to the biosynthesis of these compounds and we attempted to outline a possible route for bacterial synthesis. This study demonstrated the extended metabolic capability of Pseudomonas aeruginosa in synthesizing new chemotypes of bioactive compounds.

Erratum to “Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste” [Bioresour. Technol. 98(5) (2007) 1149–1153]

2009 ◽  
Vol 100 (23) ◽  
pp. 6141 ◽  
Author(s):  
Benjamas Thanomsub ◽  
Wanna Pumeechockchai ◽  
Anirut Limtrakul ◽  
Panarat Arunrattiyakorn ◽  
Wipawan Petchleelaha ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Chemical Structures

Chemical Characterization and Physical and Biological Activities of Rhamnolipids Produced by Pseudomonas aeruginosa BN10

2011 ◽  
Vol 66 ◽  
pp. 0394 ◽  
Author(s):  
N. Christova ◽  
B. Tuleva ◽  
R. Cohen ◽  
G. Ivanova ◽  
G. Stoev ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Chemical Characterization

scholarly journals (+)-Usnic Acid Isolated from the Lichen Cladonia substellata Impairs Myocardial Contractility

2017 ◽  
Vol 4 (02) ◽  
pp. e59-e65 ◽  
Author(s):  
Sílvia Mendonça ◽  
Carla de Vasconcelos ◽  
Jader Cruz ◽  
Danilo Roman-Campos ◽  
José Menezes-Filho ◽  
...  
Keyword(s):  
Water Solubility ◽  
Biological Activities ◽  
Membrane Integrity ◽  
Usnic Acid ◽  
Chemical Characterization ◽  
Antimicrobial Activities ◽  
Cell Membrane Integrity ◽  
Atrial Contraction ◽  
Molecular Inclusion ◽  
Isolated Cardiac Myocytes

AbstractThe scientific interest in (+)-usnic acid has grown because of its antitumor, cytotoxic, and antimicrobial activities as well as weight loss. However, overuse of usnic acid has been related with severe hepatotoxicity, making its use questionable. In this study, we decided to expand the knowledge of usnic acid biological activities by characterizing its effects on the mammalian myocardium as a potential pharmacological target. Usnic acid was isolated from samples of Cladonia substellata and submitted to chemical characterization. Molecular inclusion complexes of usnic acid with hydroxypropyl β-cyclodextrin were prepared to improve its water solubility. The effects of usnic acid on the atrial contractility and Ca2+ influx were carried out in the left atrium of guinea pigs and the effect of usnic acid on the L-type Ca2+ current was performed in rat ventricular cardiomyocytes enzymatically isolated. To evaluate the membrane integrity of cells subjected to usnic acid, we used histological procedures. Usnic acid reduced atrial contraction with an EC50 of 43.0±1.0 μM. This effect was related to a reduction of Ca2+ entry in myocardial cells. In isolated cardiac myocytes, usnic acid at 100 μM inhibited the L-type Ca2+ current by 73.0%. In addition, usnic acid caused an irreversible myocardial contracture, reflecting a serious disturbance of the intracellular Ca2+ homeostasis. Such an effect could not be ascribed to tissue death because cell membrane integrity was confirmed by histological observation. Taken together, our results show that usnic acid impairs cardiac function. Clearly more studies will be necessary to allow further applications of this natural product.

scholarly journals Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 13 ◽  
Author(s):  
Nassima Leulmi ◽  
Denise Sighel ◽  
Andrea Defant ◽  
Karima Khenaka ◽  
Abderrahmane Boulahrouf ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Carbon Sources ◽  
Culture Conditions ◽  
Quantitative Detection ◽  
Evaporative Light Scattering Detector ◽  
Streptomyces Species ◽  
Enhanced Production ◽  
Evaporative Light Scattering ◽  
Antitumor Properties

Nigericin, one of the main ionophoric polyethers produced by various Streptomyces strains, presents relevant biological activities including antibacterial and recently studied antitumor properties. This work describes the influence of different culture conditions on the production of this metabolite by Streptomyces sp. SF10, isolated from a semi-arid soil sample collected at Chélia Mountain, in Khenchela (Northeastern Algeria) and identified as Streptomyces youssoufiensis. The extracts from the strain, cultured in a solid state or submerged fermentation conditions, using several carbon sources at different pH values, in the presence or absence of iron (II) sulfate and in co-culture with other Streptomyces species, were analyzed using a high-performance liquid chromatography (HPLC) system equipped with an evaporative light scattering detector (ELSD). The best culture conditions provided a concentration of nigericin of 0.490 ± 0.001 mg/mL in the extract. The HPLC-ELSD method, optimized here for the quantitative detection of nigericin, can find wider applications in the analysis of several other metabolites characterized by a similar polycyclic polyether structure or, more generally, by the lack of significant chromophores in their molecular structure.

WITHDRAWN: Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste

2007 ◽  
Vol 98 (5) ◽  
pp. 1149-1153 ◽  
Author(s):  
Benjamas Thanomsub ◽  
Wanna Pumeechockchai ◽  
Anirut Limtrakul ◽  
Panarat Arunrattiyakorn ◽  
Wipawan Petchleelaha ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biological Activities ◽  
Chemical Structures
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