Gastroprotective Effect of an Ethanolic Extract from Neoglaziovia variegata (Arruda) Mez (Bromeliaceae) in Rats and Mice

2013 ◽  
Vol 68 (3-4) ◽  
pp. 97-107 ◽  
Author(s):  
Flávia Danniele F. Machado ◽  
Francilene V. Silva ◽  
Hélio B. Fernandes ◽  
Flávia Franceli B. P. Freitas ◽  
Daniel D. R. Arcanjo ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ischemia Reperfusion ◽  
Healing Process ◽  
Gastric Wall ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Vehicle Group ◽  
Gastroprotective Effect ◽  
Oxide Synthase ◽  
Rats And Mice

This study investigates the gastroprotective effect of a crude ethanolic extract of Neoglaziovia variegata (Arruda) Mez (Bromeliaceae), designated Nv-EtOH, in experimental models of gastric ulcer. In the ethanol-induced gastric ulcer model, Nv-EtOH showed gastroprotection at doses of 200 and 400 mg/kg body weight (BW) (57.0% and 79.7%, respectively). Nv-EtOH also significantly reduced the formation of gastric lesions induced by ethanol/HCl (31.6% and 63.5%), ibuprofen (70.0% and 74.3%), or ischemia/reperfusion in rats (65.0% and 87.0%) at 200 and 400 mg/kg BW when compared with the vehicle group. In the antioxidant activity assessment, Nv-EtOH (400 mg/kg BW) increased the catalase activity and sulfhydryl groups (SH) levels, respectively. Moreover, gastroprotection against ethanol damage was decreased after ibuprofen pretreatment. Nv-EtOH (400 mg/kg BW) promoted a significant increase in the content of gastric wall mucus. The Nv-EtOH effect was significantly reduced in mice pretreated with NG-nitro-L-arginine (L-NOARG) or glibenclamide, inhibitors of nitric oxide synthase and KATP channel activation, respectively, suggesting the involvement of these mechanisms in the Nv-EtOH-induced gastroprotective effect. Nv-EtOH decreased the total acidity, but did not modify other gastric juice parameters. Nv-EtOH was also effective in promoting the healing process in chronic gastric ulcer induced by acetic acid in rats.

scholarly journals Protective Effect of Ocotillol, the Derivate of Ocotillol-Type Saponins in Panax Genus, against Acetic Acid-Induced Gastric Ulcer in Rats Based on Untargeted Metabolomics

2020 ◽  
Vol 21 (7) ◽  
pp. 2577 ◽  
Author(s):  
Cuizhu Wang ◽  
Yuze Yuan ◽  
He Pan ◽  
Alan Chen-Yu Hsu ◽  
Jinluan Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Gastric Ulcer ◽  
Growth Factor ◽  
Gastric Mucosa ◽  
Epidermal Growth Factor ◽  
Serum Levels ◽  
Gastroprotective Effect ◽  
Epidermal Growth ◽  
Oxide Synthase

Gastric ulcer (GU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Finding a natural drug with a gastroprotective effect is needed. Ocotillol, the derivate of ocotillol-type saponins in the Panax genus, possesses good anti-inflammatory activity. The study aimed to investigate the gastroprotective effect of ocotillol on acetic acid-induced GU rats. The serum levels of endothelin-1 (ET-1) and nitric oxide (NO), the gastric mucosa levels of epidermal growth factor, superoxide dismutase and NO were assessed. Hematoxylin and eosin staining of gastric mucosa for pathological changes and immunohistochemical staining of ET-1, epidermal growth factor receptors and inducible nitric oxide synthase were evaluated. A UPLC-QTOF-MS-based serum metabolomics approach was applied to explore the latent mechanism. A total of 21 potential metabolites involved in 7 metabolic pathways were identified. The study helps us to understand the pathogenesis of GU and to provide a potential natural anti-ulcer agent.

Gastroprotective Effect of an Ethanolic Extract from Neoglaziovia variegata (Arruda) Mez (Bromeliaceae) in Rats and Mice

2013 ◽  
Vol 68 ◽  
pp. 0097 ◽  
Author(s):  
Flávia Danniele F. Machado ◽  
Francilene V. Silva ◽  
Hélio B. Fernandes ◽  
Flávia Franceli B. P. Freitas ◽  
Daniel D. R. Arcanjo ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Gastroprotective Effect ◽  
Rats And Mice

scholarly journals Gastroprotective Effect of Polypeptide-K Isolated from Momordica charantia’s Seeds on Multiple Experimental Gastric Ulcer Models in Rats

2022 ◽  
Vol 2022 ◽  
pp. 1-11
Author(s):  
Nurul ’Ain Abu Bakar ◽  
Muhammad Nazrul Hakim Abdullah ◽  
Vuanghao Lim ◽  
Yoke Keong Yong
Keyword(s):  
Gastric Ulcer ◽  
Gastric Wall ◽  
Mucus Secretion ◽  
Health Concern ◽  
Inhibition Rate ◽  
Sprague Dawley ◽  
Ulcer Disease ◽  
Gastroprotective Effect ◽  
Significant Public Health ◽  
Pyloric Ligation

Peptic ulcer disease is a multifactorial disorder and is the most significant public health concern nowadays. Previous study showed that essential oil extracted from Momordica charantia’s seed exhibited gastroprotective effect. However, the evidence for the gastroprotective effect of its active compound, polypeptide K (PPK), remains unclear. This study aimed to examine the preventive effect of PPK against different experimental gastric lesions models in rats. The possible gastroprotective effect of PPK was assessed in hydrochloride ethanol- and indomethacin-induced gastric ulcer models in Sprague Dawley rats and was further evaluated macroscopically and microscopically. Pyloric ligation experiments were used to investigate gastric secretion. Oral administration of PPK at all concentrations (10, 25, and 50 mg/kg) showed significant p < 0.05 reduction in total area of lesion in both hydrochloride ethanol- and indomethacin-induced gastric ulcer models. The highest inhibition rate was seen in PPK dose of 50 mg/kg with 64.9% and 72.2% on hydrochloride ethanol and indomethacin models, respectively. Microscopically, PPK preserved the normal architectures of the gastric tissues from being damaged by hydrochloride ethanol and indomethacin. Further, in the pyloric ligation studies, PPK significantly p < 0.05 decreased the ulcer area where the highest protection was exhibited by 50 mg/kg with 70% inhibition rate. Moreover, all concentrations of PPK also significantly p < 0.05 enhanced the gastric wall mucus secretion. Collectively, this study demonstrated the gastroprotective effect of PPK on hydrochloride ethanol- and indomethacin-induced gastric ulcer models. The possible mechanism might be associated with enhanced mucus secretion and thus lowering the total acidity.

Gastroprotective effect of aparisthman, a diterpene isolated from Aparisthmium cordatum, on experimental gastric ulcer models in rats and mice

Phytomedicine ◽  
2001 ◽  
Vol 8 (2) ◽  
pp. 94-100 ◽  
Author(s):  
C.A. Hiruma-Lima ◽  
J.S. Gracioso ◽  
W. Toma ◽  
A.B. Almeida ◽  
A.C. Paula ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Experimental Gastric Ulcer ◽  
Gastroprotective Effect ◽  
Rats And Mice

scholarly journals Development of new gastric ulcer model induced by ischemia-reperfusion and its application to evaluate the efficacy.

1996 ◽  
Vol 71 ◽  
pp. 202
Author(s):  
Kouichirou Wada ◽  
Yoshinori Kamisaki ◽  
Masayuki Kitano ◽  
Yosuke Kishimoto ◽  
Kentaro Nakamoto ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ischemia Reperfusion ◽  
Ulcer Model

Role of TLR-4/IL-6/TNF-α, COX-II and eNOS/iNOS pathways in the impact of carvedilol against hepatic ischemia reperfusion injury

2021 ◽  
pp. 096032712199944
Author(s):  
Mohamed IA Hassan ◽  
Fares EM Ali ◽  
Abdel-Gawad S Shalkami
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Liver Enzymes ◽  
Ischemia Reperfusion ◽  
Hepatic Ischemia ◽  
Hepatic Ischemia Reperfusion ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Aim: Hepatic ischemia/reperfusion (I/R) injury is a syndrome involved in allograft dysfunction. This work aimed to elucidate carvedilol (CAR) role in hepatic I/R injury. Methods: Male rats were allocated to Sham group, CAR group, I/R group and CAR plus I/R group. Rats subjected to hepatic ischemia for 30 minutes then reperfused for 60 minutes. Oxidative stress markers, inflammatory cytokines and nitric oxide synthases were measured in hepatic tissues. Results: Hepatocyte injury following I/R was confirmed by a marked increase in liver enzymes. Also, hepatic I/R increased the contents of malondialdehyde however decreased glutathione contents and activities of antioxidant enzymes. Furthermore, hepatic I/R caused elevation of toll-like receptor-4 (TLR-4) expression and inflammatory mediators levels such as tumor necrosis factor-α, interleukin-6 and cyclooxygenase-II. Hepatic I/R caused down-regulation of endothelial nitric oxide synthase and upregulation of inducible nitric oxide synthase expressions. CAR treatment before hepatic I/R resulted in the restoration of liver enzymes. Administration of CAR caused a significant correction of oxidative stress and inflammation markers as well as modulates the expression of endothelial and inducible nitric oxide synthase. Conclusions: CAR protects liver from I/R injury through reduction of the oxidative stress and inflammation, and modulates endothelial and inducible nitric oxide synthase expressions.

scholarly journals Anti-Inflammatory Effect of Phytoncide in an Animal Model of Gastrointestinal Inflammation

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1895
Author(s):  
Azra Memon ◽  
Bae Yong Kim ◽  
Se-eun Kim ◽  
Yuliya Pyao ◽  
Yeong-Geun Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Animal Model ◽  
Gastric Ulcer ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Inos Expression ◽  
Gastrointestinal Inflammation ◽  
Anti Inflammatory ◽  
Oxide Synthase ◽  
Inflammatory Effect

Background: Phytoncide is known to have antimicrobial and anti-inflammatory properties. Purpose: This study was carried out to confirm the anti-inflammatory activity of two types of phytoncide extracts from pinecone waste. Methods: We made two types of animal models to evaluate the efficacy, an indomethacin-induced gastroenteritis rat model and a dextran sulfate sodium-induced colitis mouse model. Result: In the gastroenteritis experiment, the expression of induced-nitric oxide synthase (iNOS), a marker for inflammation, decreased in the phytoncide-supplemented groups, and gastric ulcer development was significantly inhibited (p < 0.05). In the colitis experiment, the shortening of the colon length and the iNOS expression were significantly suppressed in the phytoncide-supplemented group (p < 0.05). Conclusions: Through this study, we confirmed that phytoncide can directly inhibit inflammation in digestive organs. Although further research is needed, we conclude that phytoncide has potential anti-inflammatory properties in the digestive tract and can be developed as a functional agent.

scholarly journals Ischemia-Reperfusion Injuries Assessment during Pancreas Preservation

2021 ◽  
Vol 22 (10) ◽  
pp. 5172
Author(s):  
Thomas Prudhomme ◽  
John F. Mulvey ◽  
Liam A. J. Young ◽  
Benoit Mesnard ◽  
Maria Letizia Lo Faro ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Ischemia Reperfusion ◽  
Graft Function ◽  
Impedance Analysis ◽  
Experimental Models ◽  
Caspase Activation ◽  
Pancreas Perfusion ◽  
Number Of Factors ◽  
Pancreas Preservation ◽  
Metabolite Concentrations

Maintaining organ viability between donation and transplantation is of critical importance for optimal graft function and survival. To date in pancreas transplantation, static cold storage (SCS) is the most widely practiced method of organ preservation. The first experiments in ex vivo perfusion of the pancreas were performed at the beginning of the 20th century. These perfusions led to organ oedema, hemorrhage, and venous congestion after revascularization. Despite these early hurdles, a number of factors now favor the use of perfusion during preservation: the encouraging results of HMP in kidney transplantation, the development of new perfusion solutions, and the development of organ perfusion machines for the lung, heart, kidneys and liver. This has led to a resurgence of research in machine perfusion for whole organ pancreas preservation. This review highlights the ischemia-reperfusion injuries assessment during ex vivo pancreas perfusion, both for assessment in pre-clinical experimental models as well for future use in the clinic. We evaluated perfusion dynamics, oedema assessment, especially by impedance analysis and MRI, whole organ oxygen consumption, tissue oxygen tension, metabolite concentrations in tissue and perfusate, mitochondrial respiration, cell death, especially by histology, total cell free DNA, caspase activation, and exocrine and endocrine assessment.

scholarly journals Selective A2A adenosine receptor activation reduces skin pressure ulcer formation and inflammation

2001 ◽  
Vol 281 (1) ◽  
pp. H67-H74 ◽  
Author(s):  
Shayn M. Peirce ◽  
Thomas C. Skalak ◽  
Jayson M. Rieger ◽  
Timothy L. Macdonald ◽  
Joel Linden
Keyword(s):  
Blood Flow ◽  
Pressure Ulcer ◽  
Cell Activation ◽  
Ischemia Reperfusion ◽  
Clinical Problem ◽  
Receptor Activation ◽  
Metal Plate ◽  
Vehicle Group ◽  
Ulcer Formation ◽  
A2a Adenosine Receptor

Activation of A2A adenosine receptors (A2A-AR) by ATL-146e (formerly DWH-146e) prevents inflammatory cell activation and adhesion. Recurrent ischemia-reperfusion (I/R) of the skin results in pressure ulcer formation, a major clinical problem. ATL-146e was evaluated in a novel reproducible rat model of pressure ulcer. A 9-cm2 region of dorsal rat skin was cyclically compressed at 50 mmHg using a surgically implanted metal plate and an overlying magnet to generate reproducible tissue necrosis. Osmotic minipumps were implanted into 24 rats divided into four equal groups to infuse vehicle (control), ATL-146e (0.004 μg · kg−1 · min−1), ATL-146e plus an equimolar concentration of A2A antagonist, ZM-241385, or ZM-241385 alone. Each group received 10 I/R cycles. In non-I/R-treated skin, ATL-146e has no effect on blood flow. I/R-treated skin of the ATL-146e group compared with the vehicle group had 65% less necrotic area, 31% less inhibition of average skin blood flow, and fewer extravasated leukocytes (23 ± 3 vs. 49 ± 6 per 500 μm2). These data suggest that ATL-146e, acting via an A2A-AR, reduces leukocyte infiltration and is a potent prophylactic for I/R injury in skin.

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