Standard Guide for Retroreflective Composite Optics Laboratory Procedures

Background: Serum Peanut-specific-IgE (PN-sIgE) and peanut-component-resolved-diagnostics (CRD) are often ordered simultaneously in the evaluation for peanut allergy. Results often guide the plans for peanut oral challenge. However, the clinical utility of CRD at different total PN-sIgE levels is unclear. A commonly used predefined CRD Ara h2 cutoff value in the literature predicting probability of peanut challenge outcomes is 0.35kUA/L. Objective: To examine the utility of CRD in patients with and without a history of clinical reactivity to peanut (PN). Methods: This was a retrospective chart review of 196 children with PN-sIgE and CRD testing, of which, 98 patients had a clinical history of an IgE-mediated reaction when exposed to PN and 98 did not. The Fisher's exact test was used to assess the relationship between CRD and PN-sIgE at different cutoff levels, McNemar test and Gwet’s approach (AC1 statistic) were used to examine agreement between CRD and PN-sIgE, and logistic regression was used to assess differences in the findings between patients with and without reaction history. Results: Ara h 1, 2, 3, or 9 (ARAH) levels ≤0.35 kUA/L were significantly associated with PN-sIgE levels <2 kUA/L rather than ≥2 kUA/L (p < 0.0001). When the ARAH threshold was increased to 1 kUA/L and 2 kUA/L, these thresholds were still significantly associated with PN-sIgE levels of <2, <5, and <14 kUA/L. These findings were not significantly different in patients with and without a history of clinical reactivity. Conclusion: ARAH values correlated with PN-sIgE. Regardless of clinical history, ARAH levels are unlikely to be below 0.35, 1, or 2 kUA/L if the PN-sIgE level is >2 kUA/L. Thus, if possible, practitioners should consider PN-sIgE rather than automatically ordering CRD with PN-sIgE every time. Laboratory procedures that allow automatically and reflexively adding CRD when the PN-sIgE level is ≤5 kUA/L can be helpful. However, further studies are needed in subjects with challenge-proven PN allergy.

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Effects of laboratory procedures on intrauterine insemination success

10.26226/morressier.573c1514d462b80296c98db6 ◽

2016 ◽

Author(s):

Louise Lemmens

Keyword(s):

Intrauterine Insemination ◽

Laboratory Procedures

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Water quality. Guidance on field and laboratory procedures for quantitative analysis and identification of macroinvertebrates from inland surface waters

10.3403/30340633u ◽

2019 ◽

Keyword(s):

Water Quality ◽

Quantitative Analysis ◽

Surface Waters ◽

Laboratory Procedures

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A Simple Technique to Fabricate Bite Turbos

The Journal of Indian Orthodontic Society ◽

10.1177/03015742211013762 ◽

2021 ◽

pp. 030157422110137

Author(s):

Hoger Omran Alsheikho ◽

Doaa Jomah

Keyword(s):

Simple Technique ◽

Resin Composite ◽

Dental Practice ◽

Posterior Teeth ◽

Laboratory Procedures

Bite turbos are a relatively new but widespread fabrication used in a variety of orthodontic cases. Bite turbos placed on lingual surfaces of upper incisors help disclude the posterior teeth and open the bite. This report describes a simple, effective, and economical technique to fabricate resin composite bite turbos. This method does not require laboratory procedures and can be manufactured using materials that are usually available in a regular dental practice.

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The Use of Lasers in Dental Materials: A Review

Materials ◽

10.3390/ma14123370 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3370

Author(s):

Emmanouil-George C. Tzanakakis ◽

Evangelos Skoulas ◽

Eudoxie Pepelassi ◽

Petros Koidis ◽

Ioannis G. Tzoutzas

Keyword(s):

Energy Levels ◽

Dental Materials ◽

High Strength ◽

Surface Characteristics ◽

High Energy ◽

Zirconia Ceramics ◽

Material Management ◽

Laboratory Procedures ◽

Laser Devices ◽

Exact Point

Lasers have been well integrated in clinical dentistry for the last two decades, providing clinical alternatives in the management of both soft and hard tissues with an expanding use in the field of dental materials. One of their main advantages is that they can deliver very low to very high concentrated power at an exact point on any substrate by all possible means. The aim of this review is to thoroughly analyze the use of lasers in the processing of dental materials and to enlighten the new trends in laser technology focused on dental material management. New approaches for the elaboration of dental materials that require high energy levels and delicate processing, such as metals, ceramics, and resins are provided, while time consuming laboratory procedures, such as cutting restorative materials, welding, and sintering are facilitated. In addition, surface characteristics of titanium alloys and high strength ceramics can be altered. Finally, the potential of lasers to increase the adhesion of zirconia ceramics to different substrates has been tested for all laser devices, including a new ultrafast generation of lasers.

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Selected In Situ Hybridization Methods: Principles and Application

Molecules ◽

10.3390/molecules26133874 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3874

Author(s):

Dominika Veselinyová ◽

Jana Mašlanková ◽

Katarina Kalinová ◽

Helena Mičková ◽

Mária Mareková ◽

...

Keyword(s):

In Situ Hybridization ◽

Imaging Techniques ◽

Cell Communication ◽

Probe Design ◽

Rapid Progress ◽

In Situ Techniques ◽

Different Types ◽

Laboratory Procedures ◽

The Individual

We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level.

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Unveiling the Link between the Third Law of Comminution and the Grinding Kinetics Behaviour of Several Ores

Metals ◽

10.3390/met11071079 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1079

Author(s):

Victor Ciribeni ◽

Juan M. Menéndez-Aguado ◽

Regina Bertero ◽

Andrea Tello ◽

Enzo Avellá ◽

...

Keyword(s):

Standard Procedure ◽

Research Work ◽

Excellent Correlation ◽

The Third ◽

Work Index ◽

Bond Work Index ◽

Laboratory Procedures ◽

Grinding Kinetics ◽

Definition Of ◽

Index Estimation

As a continuation of a previous research work carried out to estimate the Bond work index (wi) by using a simulator based on the cumulative kinetic model (CKM), a deeper analysis was carried out to determine the link between the kinetic and energy parameters in the case of metalliferous and non-metallic ore samples. The results evidenced a relationship between the CKM kinetic parameter k and the grindability index gbp; and also with the wi, obtained following the standard procedure. An excellent correlation was obtained in both cases, posing the definition of alternative work index estimation tests with the advantages of more straightforward and quicker laboratory procedures.

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Recommended Procedures to Assess Critical State Locus from Triaxial Tests in Cohesionless Remoulded Samples

Geotechnics ◽

10.3390/geotechnics1010006 ◽

2021 ◽

Vol 1 (1) ◽

pp. 95-127

Author(s):

António Viana da Fonseca ◽

Diana Cordeiro ◽

Fausto Molina-Gómez

Keyword(s):

Critical State ◽

Review Paper ◽

State Theory ◽

Soil Freezing ◽

Triaxial Tests ◽

Cohesionless Soils ◽

Saturation Condition ◽

Testing Procedures ◽

Laboratory Procedures ◽

Soil Behaviour

The critical state theory is a robust conceptual framework for the characterisation of soil behaviour. In the laboratory, triaxial tests are used to assess the critical state locus. In the last decades, the equipment and testing procedures for soil characterisation, within the critical state framework, have advanced to obtain accurate and reliable results. This review paper summarises and describes a series of recommended laboratory procedures to assess the critical state locus in cohesionless soils. For this purpose, results obtained in the laboratory from different cohesionless soils and triaxial equipment configurations are compiled, analysed and discussed in detail. The procedures presented in this paper reinforce the use of triaxial cells with lubricated end platens and an embedded connection piston into the top-cap, together with the verification of the full saturation condition and the measurement end-of-test water content—preferable using the soil freezing technique. The experimental evidence and comparison between equipment configurations provide relevant insights about the laboratory procedures for obtaining a reliable characterisation of the critical state locus of cohesionless geomaterials. All the procedures recommended herein can be easily implemented in academic and commercial geotechnical laboratories.

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