Effects of Distillers Grains and Substrate Steam-Flaked Corn Concentration on In Vitro Dry Matter Disappearance, Gas Production Kinetics, and Hydrogen Sulfide Production1

2010 ◽  
Vol 26 (4) ◽  
pp. 365-374 ◽  
Author(s):  
M.J. Quinn ◽  
M.L. May ◽  
N. DiLorenzo ◽  
D.R. Smith ◽  
M.L. Galyean
Keyword(s):  
Hydrogen Sulfide ◽  
Dry Matter ◽  
Gas Production ◽  
Distillers Grains ◽  
Production Kinetics

Method for measuring gas production kinetics

1998 ◽  
Vol 22 ◽  
pp. 209-211 ◽  
Author(s):  
D. R. Mertens ◽  
P. J. Weimer
Keyword(s):  
Kinetic Parameters ◽  
Dry Matter ◽  
Critical Role ◽  
Gas Production ◽  
Production Kinetics ◽  
Digestion Kinetics ◽  
Ruminal Digestion ◽  
Kinetics Of ◽  
Or Modelling

Methodology can play a critical rôle in the measurement of digestion kinetics, especially when the objective is to define kinetic parameters for use in formulating rations or modelling animal responses. Measurement of gas production kinetics provides the opportunity to evaluate the rate of digestion of the soluble, more rapidly fermenting fractions of foods but has the potential for being more sensitive to the in vitro procedure used. Differences among procedures that have little impact on digestion of dry matter after 48 h of incubation, may have dramatic effects on fermentation of soluble matter during the first 20 h. Our objective was to develop a method for measuring the kinetics of gas production that would minimize any detrimental effects associated with the in vitro system and provide estimates of digestion kinetics that can be used to both describe foods for ration formulation systems and provide parameters for models of ruminal digestion.

scholarly journals 134 Effects of Aspergillus oryzae prebiotic on in vitro digestibility and fermentability of fibrous ingredients and diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 110-110
Author(s):  
Jinlong Zhu ◽  
Jae-Cheol Jang ◽  
Gerald C Shurson ◽  
Lynsey Whitacre ◽  
Ignacio R Ipharraguerre ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Mixed Model ◽  
Enzymatic Digestion ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Production Kinetics ◽  
In Vitro Hydrolysis

Abstract It has been an increasing interest to develop strategies to improve utilization of fibrous ingredients in swine. The objective of this study was to determine the effects of an Aspergillus oryzae prebiotic (AOP, Amaferm®) on in vitro digestibility and fermentability of energy and dry matter using a 3-step in vitro enzymatic digestion and fermentation. Three diets were formulated based on corn-DDGS (DDGS), rice bran (RB), and wheat middlings (WM). During in vitro hydrolysis and fermentation, 0.05% AOP was added to the diets (DDGS+AOP, RB+AOP, and WM+AOP) and sole ingredients. Each 2-g sample was hydrolyzed for 2 h with pepsin and for a subsequent 4 h with pancreatin. Hydrolyzed residues were filtered, washed, dried, weighed, pooled within the same sample, and used for subsequent fermentation using swine fecal inoculum. Volume of gas produced was recorded at 11 time points during 72 h of incubation. Parameters of gas production kinetics were calculated using a nonlinear monophasic model, and differences among treatment groups were compared using a mixed model. Supplementation of AOP increased (P < 0.05) in vitro digestibility of gross energy (IVDGE) and dry matter (IVDDM) in the diets and AOP tended to increase (P = 0.06) dry matter fermentability of the diets. Supplementation of AOP during fermentation did not (P > 0.1) affect maximum gas production in ingredients or diets. AOP decreased the half-time to asymptote (T/2) in the ingredient of DDGS+AOP compared with that of DDGS and increased the fraction rate of degradation at T/2 in the diet of RB+AOP compared with that of RB. In conclusion, supplementation of AOP increased energy and dry matter digestibility of the diets during in vitro hydrolysis and modified gas production kinetics during in vitro fermentation and the effects were dietary- and ingredient-type dependent.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

scholarly journals Effects of exogenous enzymes on in vitro gas production kinetics and ruminal fermentation of four fibrous feeds

2013 ◽  
Vol 179 (1-4) ◽  
pp. 46-53 ◽  
Author(s):  
M.M.Y. Elghandour ◽  
A.Z.M. Salem ◽  
M. Gonzalez-Ronquillo ◽  
J.L. Bórquez ◽  
H.M. Gado ◽  
...  
Keyword(s):  
Gas Production ◽  
Ruminal Fermentation ◽  
Production Kinetics ◽  
In Vitro Gas Production ◽  
Exogenous Enzymes

Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

2021 ◽  
pp. 1-10
Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Gas Production ◽  
Rapid Decline ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Acid Detergent Fibre ◽  
Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

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