ISOLATION AND IDENTIFICATION OF 16-OXOOESTRADIOL-17β IN HUMAN PLACENTAE

1959 ◽  
Vol XXXII (II) ◽  
pp. 195-208 ◽  
Author(s):  
E. Diczfalusy ◽  
Anne-Marie v. Münstermann
Keyword(s):  
Ethanol Extract ◽  
Full Term ◽  
Free Form ◽  
Isolation And Identification ◽  
Human Placentae

ABSTRACT From the ethanol extract of 100 full term placentae approximately 200 μg of an α-ketolic Kober chromogen have been isolated in a »free« form. The evidence obtained indicates that this α-ketolic Kober chromogen is identical with 16-oxooestradiol-17β.

scholarly journals Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum

2018 ◽  
Vol 16 (1) ◽  
pp. 324-332 ◽  
Author(s):  
Nurhayat Tabanca ◽  
Zulfiqar Ali ◽  
Ulrich R. Bernier ◽  
Nancy Epsky ◽  
Ayse Nalbantsoy ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Ethanol Extract ◽  
Ethyl Linoleate ◽  
Significant Activity ◽  
Isolation And Identification ◽  
Human Carcinoma ◽  
Ethyl Palmitate ◽  
H Nmr ◽  
Bioassay Guided Fractionation ◽  
Nmr Techniques

AbstractAn ethanol extract fromVeratrum lobelianumBernh. rhizomes showed larvicidal activity with LC50values of 11.79 ppm and 89.9 ppm against 1stand 4thinstar larvae, respectively, at 24 h post-treatment. The extract also showed proportion not biting (PNB) value of 0.76 at 100 μg/cm2against females ofAe.aegypti. Systematic bioassay-guided fractionation ofV.lobelianumextract resulted in the isolation of five compounds that were identified as ethyl palmitate (1), ethyl linoleate (2), β-sitosterol (3), resveratrol (4) and oxyresveratrol (5) by GC-MS,1H-NMR, and13C-NMR techniques, comparison with literature data, and confirmation with authentic compounds. Compound2exhibited larvicidal activity with an LC50value of 24.1 (22.0-26.2) ppm whereas1was inactive. β-Sitosterol (3) displayed the highest larvicidal activity with LC50= 1.7 (1.3-12.3) ppm and LC90= 5.1 (3.4-13.8) ppm. Compounds4and5had larvicidal activity with LC50values of 18.5 (15.3-23.3) and 22.6 (19.0-26.8) ppm, respectively, and had an equivalent PNB values of 0.75 at 25 nmol/cm2. In addition,4and5were explored for their human-based repellency againstAe.aegypti, attractiveness against male medfliesCeratitis capitata, and also evaluated against series of human carcinoma cells (A549, HEK293, HeLa, SH-SY5Y); however, no significant activity was found.

scholarly journals Isolation and Identification of the Anti-Oxidant Constituents from Loropetalum chinense (R. Brown) Oliv. Based on UHPLC–Q-TOF-MS/MS

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1720 ◽  
Author(s):  
Haifang Chen ◽  
Mulan Li ◽  
Chen Zhang ◽  
Wendi Du ◽  
Haihua Shao ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Control Group ◽  
Ethyl Gallate ◽  
Isolation And Identification ◽  
Caffeoylquinic Acid ◽  
Tof Ms

The aim of this study was to identify the chemical constituents of Loropetalum chinense (R. Brown) Oliv. (LCO) and determine which of these had antioxidant effects. The chemical composition of a 70% ethanol extract of LCO was analyzed systematically using UHPLC–Q-TOF-MS/MS. The chemical components of the 70% ethanol extract of LCO were then separated and purified using macroporous resin and chromatographic techniques. Antioxidant activity was evaluated using a DPPH assay. In total, 100 compounds were identified tentatively, including 42 gallic acid tannins, 49 flavones, and 9 phenolic compounds. Of these, 7 gallium gallate, 4 flavonoid and 8 quinic acid compounds were separated and purified from the 70% ethanol extract of LCO. The compounds identified for the first time in LCO and in the genus Loropetalum were 3,4,5-trimethoxyphenyl-(6′-O-galloyl)-O-β-d-glucopyranoside, protocatechuic acid, ethyl gallate, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3,5-O-diocaffeoylquinic acid, 4,5-O-diocaffeoylquinic acid and 3,4-O-diocaffeoylquinic acid. The 50% inhibitory concentration (IC50) values of compounds 1,2,3,4,6-penta-O-galloyl-β-d-glucose, gallic acid, protocatechuic acid, and ethyl gallate were 1.88, 1.05, 1.18, and 1.05 μg/mL, respectively. Compared with the control group (VC) (2.08 μg/mL), these compounds exhibited stronger anti-oxidation activity. This study offered considerable insight into the chemical composition of LCO, with preliminary identification of the antioxidant ingredients.

LEVELS AND DISTRIBUTION OF FREE AND C4b-BP-B0UND-PR0TEIN S IN HUMAN FETUSES AND FULL-TERM NEWBORNS

1987 ◽  
Author(s):  
P MOALIC ◽  
Y GRUEL ◽  
P FOLOPPE ◽  
B DELAHOUSSE ◽  
G BODY ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Protein S ◽  
Full Term ◽  
Free Form ◽  
Reference Curve ◽  
Direct Puncture ◽  
A Value ◽  
Term Newborns ◽  
Low Levels ◽  
Normal Adults

Levels and plasmatic distribution of protein S were studied on umbilical cordplasmas from 25 normal full-term newborns (N) and 15 normal fetuses (F) between20 and 30 weeks of gestation. Samples from fetuses were collected for antenatal diagnosis by direct puncture of the umbilical vein under high resolution real-time ultrasound. Total protein S(PS) level was determined using Laurell rocket immuno-electrophoresis (Diagnostica Stago, Asnifcres-France). Free PS wasmeasured using this latter method, afterprecipitation of C4b-BP-bound-PS by polyethylene glycol (PEG). Normal pool plasma, treated as well, was considered as the reference curve. C4b-binding protein (C4b-BP) determinations were conducted by Laurell rocket immunoelectrophoresis. The qualitative distribution of free PS and C4b-BP-bound-PS in plasma was also assessed by crossed-immunoelectrophoresis(CIE). Results (mean - SD) were expressed in percentage, in relation to healthy adults values (n = 15). Low levels of total PS were obtained in all fetuses (16.4 ±4.2) and newborns (36.4 ±9.5) as compared to adults (91.6 ± 12.2). Free protein S level was also decreased both in fetuses (22.2 ±6.0) and newborns (48.5 ± 12.1 versus 89.4 ± 26.3 in adults). At these stages of development, the ratio Free PS / Total PS (both values were obtained according to a reference curve performed with a normal adult pool plasma untreated by PEG) was significantly higher as compared to normal adults (0.82 ±0.07 in F, 0.64 ±0.17 in N and 0.39 ±0.11 in A, p‹0.001, Student t test). The predominance of free PS was also visualized in the CIE patterns. These data may be explained by undetectable C4b-BP in 21-week old fetuses (‹2% in 10 cases). After the 26th week of gestation C4b-BP level was 7.8 ±7.4 ‹n=5) and reached a value of 19.2 ±15.6 in newborns (adults = 95.7 ±14.7). In human fetus and newborn, PS essentially circulates under free form and this might compensate the decrease of the total PS level.

scholarly journals UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Ni Made Dwi Desiyanti ◽  
I Made Dira Swantara ◽  
I Putu Sudiarta
Keyword(s):  
Ir Spectra ◽  
Silica Gel ◽  
Column Chromatography ◽  
Myzus Persicae ◽  
Mobile Phase ◽  
Ethanol Extract ◽  
Annona Muricata ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Metabolite Extraction

The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.

scholarly journals Isolation and Identification of Insect Antifeedant Compound from Ethanol Extract of Hemidesmus indicus Root

2020 ◽  
Author(s):  
Maya G. Pillai, Susha Dayanandan, Beena Bendada ◽  
Ali Guendouz ◽  
Ramdane Benniou
Keyword(s):  
Insect Pest ◽  
Ethanol Extract ◽  
Synergistic Action ◽  
Corcyra Cephalonica ◽  
Stored Grain ◽  
Isolation And Identification ◽  
Hemidesmus Indicus ◽  
Antifeedant Effect ◽  
Grain Protectants

Phytochemicals with insect antifeedant potential can be used as a safer alternative to harmful chemicals that are used as grain protectants. The insect antifeedant effect of the extracts and fractions of Hemidesmus indicus root were tested against the stored grain insect pest Corcyra cephalonica Stainton. Bioactivity-guided study of ethanol extract of Hemidesmus indicus root led to isolation and identification of a triterpenoid, Lupeol with insect antifeedant potential. Although Lupeol showed insect antifeedant potential the ethanol extract was found to be more effective as an antifeedant. This implies that the synergistic action of compounds present in the ethanol extract of H. indicus root is responsible for the higher antifeedant potential.

scholarly journals Anti-inflammatory Activity of Sclerotium stipitatum Berk. et. Curr. an Ethnomedicinal Fungus, in Chronic and Acute Animal Models of Inflammation

2021 ◽  
pp. 209-218
Author(s):  
Alina K. Sebastian ◽  
P. V. Anto
Keyword(s):  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Isolation And Identification ◽  
Standard Drug ◽  
Significant Inhibitory Effect ◽  
Anti Inflammatory

Sclerotium stipitatum Berk. et. Curr., locally known as ‘nilamanga’ is a rare macro fungus, traditionally used to treat numerous diseases like arthritis, earache, jaundice etc. The present study aims to evaluate the anti-inflammatory activity of ethanol extract of S. stipitatum and identify the bioactive compounds present in them. Phytochemical screening of extracts obtained using different solvents like petroleum ether, chloroform, ethanol and water were done. The best extract was chosen for the acute carrageenan-induced and chronic formalin-induced anti-inflammatory studies. Diclofenac was used as the standard drug. Ethanol extract showed significant inhibition of inflammation induced by carrageenan and formalin-induced paw edema models compared to the control. GC-MS analysis shows certain bioactive compounds. The significant inhibitory effect on paw edema proves that S. stipitatum possesses remarkable anti-inflammatory activity, and isolation and identification of bioactive compounds can be used for new drug formulations.

scholarly journals ISOLATION AND IDENTIFICATION OF SECONDARY METABOLIT COMPOUNDS IN ETIL ASETIC FRACTIONS ON MINDI LEAF

2019 ◽  
Vol 23 (2) ◽  
pp. 95-102
Author(s):  
Nurul Lutfia ◽  
Sri Atun
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Polar Fraction ◽  
Melia Azedarach ◽  
Base Peak ◽  
Isolation And Identification ◽  
Different Types ◽  
Dendrophthoe Falcata

This study was aimed at isolating and identifying secondary metabolites contained in the ethyl acetate fraction of parasitic leaves (Dendrophthoe falcata (L.f)) Ettingsh which attaches to the stem of Mindi plants (Melia azedarach). The subjects in this study were leaves of parasitic plants on Mindi plants. While the objects in this study were secondary metabolites from the ethyl acetate fraction of the leaves of parasite plants attached to the mindi plant. The research method was carried out by extracting macerated leaves of D. falcata with ethanol solvents. The ethanol extract obtained was partitioned using n-hexane, chloroform, and ethyl acetate. Ethyl acetate fraction was separated by gravity column chromatography (CCG). The relatively non-polar fraction that has shown a single stain is tested for purity using 3 different types of solvents and identified using UV-Vis, IR, and GC-MS. The results showed that the compounds that could be isolated and identified from the relatively non-polar ethyl acetate fraction of D. falcata leaves were stigmast-5-en-3β-ol with an abundance of 6.49%, having a molecular weight of m / z 414 with base peak 55. Compounds sterols are compounds commonly found in parasitic plantsISOLASI DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA FRAKSI ETIL ASETAT DAUN MINDIPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder yang terdapat dalam fraksi etil asetat daun benalu (Dendrophthoe falcata (L.f)) Ettingsh yang menempel pada batang tumbuhan Mindi (Melia azedarach). Subjek dalam penelitian ini adalah daun tumbuhan benalu pada tanaman mindi. Sedangkan objek dalam penelitian ini adalah senyawa metabolit sekunder dari fraksi etil asetat daun tumbuhan benalu yang menempel pada tanaman mindi. Metode penelitian dilakukan dengan ekstraksi maserasi daun D. falcata dengan pelarut etanol. Ekstrak etanol yang diperoleh dipartisi menggunakan n-heksana, kloroform, dan etil asetat. Fraksi etil asetat dipisahkan secara kromatografi kolom gravitasi (KKG). Fraksi relatif non polar yang sudah menunjukkan noda tunggal diuji kemurniannya menggunakan 3 jenis pelarut yang berbeda dan diidentifikasi menggunakan UV-Vis, IR, dan GC-MS. Hasil penelitian menunjukkan senyawa yang dapat diisolasi dan diidentifikasi dari fraksi etil asetat relatif nonpolar daun D. falcata adalah stigmast-5-en-3β-ol dengan kelimpahan 6,49 %, memiliki berat molekul m/z 414 dengan base peak 55. Senyawa sterol merupakan senyawa yag lazim ditemukan dalam tumbuhan benalu.  

scholarly journals Phytochemical Analysis and Antioxidant Capacity of BM-21, a Bioactive Extract Rich in Polyphenolic Metabolites from the Sea Grass Thalassia testudinum

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Erik L. Regalado ◽  
Roberto Menendez ◽  
Olga Valdés ◽  
Ruth A. Morales ◽  
Abilio Laguna ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
2D Nmr ◽  
Thalassia Testudinum ◽  
Phytochemical Analysis ◽  
Isolation And Identification ◽  
Phytochemical Investigation ◽  
Potential Applications

The aqueous ethanol extract of Thalassia testudinum leaves (BM-21) is now being developed in Cuba as an herbal medicine due to its promising pharmacological properties. Although some interesting biological activities of BM-21 have already been reported, its chemical composition remains mostly unknown. Thus, we now describe the qualitative and quantitative analyzes of BM-21 using standard phytochemical screening techniques, including colorimetric quantification, TLC and HPLC analyses. Phytochemical investigation of BM-21 resulted in the isolation and identification of a new phenolic sulfate ester (1), along with ten previously described phenolic derivatives (2-11), seven of which have never been previously reported from the genus Thalassia. The structures of these compounds were established by analysis of their spectroscopic (1D and 2D NMR) and spectrometric (HRMS) data, as well as by comparison of these with those reported in the literature. Furthermore, BM-21 was found to exhibit strong antioxidant activity in four different free radical scavenging assays (HO•, RO2•, O2-• and DPPH•). Consequently, this is the first study which highlights the phytochemical composition of BM-21 and demonstrates that this product is a rich source of natural antioxidants with potential applications in pharmaceutical, cosmetic and food industries.

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