GESTOGENS IN CORPUS LUTEUM OF CATTLE

1960 ◽  
Vol XXXIII (III) ◽  
pp. 417-427 ◽  
Author(s):  
John Kristoffersen

ABSTRACT By means of chromatographic and spectrophotometric methods progesterone and a substance closely similar to 20β-hydroxy-pregn-4-ene-3-one has been detected in luteal tissue from non-pregnant and pregnant cows. In 21 animals quantitative measurements based on a method giving an average net recovery of 56 per cent showed that in pregnancy the average progesterone content in the corpus luteum reached a maximum in the third to fifth month, with low values before and after this period. For 6 nonpregnant cows, the average value was 20.2 μg/g tissue, which is considerably higher than previous values reported in the literature. The relation between these findings and the bovine dependence on a functional corpus luteum in pregnancy is discussed, and it is pointed out that more information about the metabolism of progesterone in cattle is highly desirable.

2020 ◽  
Vol 4 (2) ◽  
pp. 173-181
Author(s):  
Siska Oktaviani ◽  
Muhammad Ikhsan ◽  
Nur Magfira Firdausetia

This study intended to improve students learning outcomes on force and motion material using the bingo game. This research was Classroom Action Research (CAR) conducted in three cycles. The subjects of the study were 26 children, consisting of 16 male students and 10 female students. Data collection techniques with observation and documentation and test learning outcomes.The results showed in the first cycle with an average value of 66, the second cycle an average value of 80.76, and the third cycle an average value of 90.38. The level of completeness in the first cycle was 38.46%, in the second cycle was 76.92%, and the third cycle increased to 92.30%. Thus, the level of completeness from cycle I to cycle II rose 30.77%, and cycle II to cycle III growth to 22.98%. Using the bingo game can increase student activity from cycle I to cycle III. In cycle I, there are aspects of attention 61.5%, activeness 38.4% and participation 46.1% have increased in cycle II on aspects of attention 73%, activeness 57.6%, 61.5% participation and increased in cycle III, namely in the aspect of attention to 92.3% activeness to 84.6% and participation aspects to 80.75%.In the assessment, there are two namely the spiritual attitude and social attitude. There are 3 aspects of spiritual attitude, namely thankful behavior, praying before and after doing activities, and saying hello when entering and leaving the room. And there are 5 aspects of social attitude, namely discipline, care, confidence, courtesy, and responsibility. It was seen that from cycle I to cycle III has increased in both good and very good categories. In the first cycle skills assessment with a percentage of 42.30%, cycle II with a percentage of 73.07%, and cycle III with a percentage of 92.30.


Reproduction ◽  
2021 ◽  
Author(s):  
Pedro L. J. Monteiro ◽  
Roberto Sartori ◽  
Aurea M O Canavessi ◽  
Leonardo F. Melo ◽  
Jessica C. L. Motta ◽  
...  

Inappropriate corpus luteum (CL) regression can produce pregnancy loss. An experimental model was utilized to investigate regression of accessory CL during pregnancy in dairy cows. Cows were bred (Day 0) and treated with GnRH 6 d later to form accessory CL. Transrectal ultrasound (every other d) and blood samples for progesterone (P4; daily) were performed until Day 56 of pregnancy. On Day 28, 13 cows were confirmed pregnant, and accessory CL were found contralateral (n=9) or ipsilateral (n=4) to previous ovulation. On Day 18, CL biopsy was performed to analyze mRNA expression for interferon-stimulated genes (ISGs). Luteolysis occurred more frequently in cows that had contralateral accessory CL [88.9% (8/9)] than cows with ipsilateral accessory CL [0% (0/4)]. Luteolysis of contralateral accessory CL occurred either earlier (Day 19-23; 2/8) or later (Day 48-53; 6/8) in pregnancy and occurred rapidly (24 h), based on daily P4. After onset of earlier or later accessory CL regression, circulating P4 decreased 41.2%. There was no difference in luteal tissue mRNA expression for ISGs on Day 19 between accessory and original CL and between CL that subsequently regressed or did not regress. On Day 56, an oxytocin challenge dramatically increased PGFM in all cows but produced no pregnancy losses, although cows with previous accessory CL regression had greater PGFM. In summary, ipsilateral accessory CL did not regress during pregnancy, whereas most contralateral CL regressed by 63 d of pregnancy, providing evidence for local mechanisms in regression of accessory CL and protection of CL of pregnancy.


1987 ◽  
Vol 26 (05) ◽  
pp. 192-197 ◽  
Author(s):  
T. Kreisig ◽  
P. Schmiedek ◽  
G. Leinsinger ◽  
K. Einhäupl ◽  
E. Moser

Using the 133Xe-DSPECT technique, quantitative measurements of regional cerebral blood flow (rCBF) were performed before and after provocation with acetazolamide (Diamox) i. v. in 32 patients without evidence of brain disease (normals). In 6 cases, additional studies were carried out to establish the time of maximal rCBF increase which was found to be approximately 15 min p. i. 1 g of Diamox increases the rCBF from 58 ±8 at rest to 73±5 ml/100 g/min. A Diamox dose of 2 g (9 cases) causes no further rCBF increase. After plotting the rCBF before provocation (rCBFR) and the Diamox-induced rCBF increase (reserve capacity, Δ rCBF) the regression line was Δ rCBF = −0,6 x rCBFR +50 (correlation coefficient: r = −0,77). In normals with relatively low rCBF values at rest, Diamox increases the reserve capacity much more than in normals with high rCBF values before provocation. It can be expected that this concept of measuring rCBF at rest and the reserve capacity will increase the sensitivity of distinguishing patients with reversible cerebrovascular disease (even bilateral) from normals.


2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.


1986 ◽  
Vol 113 (4) ◽  
pp. 570-575 ◽  
Author(s):  
Firyal S. Khan-Dawood

Abstract. Immunoreactive oxytocin is detectable in the corpora lutea of women and cynomolgus monkeys by radioimmunoassay. To localize the presence of oxytocin and neurophysin I in ovarian tissues of subhuman primates, three corpora lutea and ovarian stromal tissues and two Fallopian tubes obtained during the menstrual cycle of the baboon and decidua from two pregnant baboons were examined using highly specific antisera against either oxytocin or neurophysin I and preoxidase-antiperoxidase light microscopy immunohistochemistry. Oxytocin-like as well as neurophysin I-like immunoreactivities were found in some cells of all the corpora lutea only, but could not be demonstrated in ovarian stromal tissues, Fallopian tubes and decidua. Specificity of the immunocytochemical reaction was further confirmed by immunoabsorption of the antiserum with excess oxytocin or neurophysin, after which the immunoreactivities for both oxytocin and neurophysin in the luteal tissue were negative. Similar controls using normal rabbit serum gave no positive staining for either oxytocin or neurophysin. Counterstaining of the positive immunoreactivities for oxytocin and neurophysin I with Mayer's haematoxylin and eosin demonstrated clearly that the oxytocin and neurophysin I appeared as granular material mainly within the cytoplasm of the luteal cells. The localization of immunoreactive oxytocin and neurophysin I in the corpus luteum of the baboon demonstrates directly the presence of these two neurohypophysial peptides within primate luteal cells and suggests their local production.


Author(s):  
Matthew Hobson

This chapter provides a brief introduction to how the historiographical development of Roman studies, since mid-twentieth century decolonization, has altered our understanding of the developments which took place in North Africa following the destruction of Carthage in 146 bce. The reader is introduced to literary, epigraphic, and archaeological sources of evidence, which have traditionally been used to argue for either cultural change or continuity. After an initial examination of the immediate aftermath of the Third Punic War, Roman land appropriation and taxation, the focus is on sources of evidence usually described as “Punic,” “neo-Punic” or “Late Punic,” covering the spheres of municipal institutions, language use, and religious and funerary rituals. The vibrant multiculturalism and regional diversity of the Mediterranean and especially North Africa, both before and after the Roman conquest, is the dominant theme. This is used to shift emphasis away from grand explanatory paradigms based on essentialist identity categories, and toward a more nuanced picture of the complex and multivariate processes of cultural development and integration.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jeong Woo Kang ◽  
Yeoun Sook Chun ◽  
Nam Ju Moon

Abstract Background To evaluate the change of accommodation and ocular discomfort according to the display size, using quantitative measurements of accommodation and ocular discomfort through subjective and objective metrics. Methods Forty six subjects without any ophthalmic disease history were asked to watch the documentary movie, using two different sizes of smart devices; smartphones and tablets. Before and after using devices, the near point accommodation (NPA) and the near point convergence (NPC) were measured, and objective accommodation was measured using an auto refractometer/keratometer. The subjective ocular discomfort was assessed through a survey. Results Both devices showed a decrease in post-use NPA and NPC, and the change after use of the smartphone was significantly severe, 1.8 and 2.5 folds respectively, compared to tablet (p = 0.044, p = 0.033, respectively). Neither smartphone nor tablet showed significant changes in the accommodative response induced by dynamic accommodative stimulus of auto refractometer/keratometer (p = 0.240 and p = 0.199, respectively). Subjects showed a more severe increase in ocular discomfort after using smartphones (p = 0.035) and reported feeling tired even with shorter use times (p = 0.012). Conclusions Both devices showed significant decreases in NPA and NPC, and the larger changes were seen when using the small display smartphone. Even within 20minutes of using, subjects start to feel ocular discomfort, and it was more severe and faster after smartphones than tablets. Therefore, the smaller the display size, the greater the adverse impact on eyes, and thus, appropriate display size will need to be selected depending on the time and purpose of use.


2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.


1990 ◽  
Vol 124 (2) ◽  
pp. 225-232 ◽  
Author(s):  
J. J. Hirst ◽  
G. E. Rice ◽  
G. Jenkin ◽  
G. D. Thorburn

ABSTRACT The effect of protein kinase C activation and dibutyryl cyclic AMP on oxytocin secretion by ovine luteal tissue slices was investigated. Several putative regulators of luteal oxytocin secretion were also examined. Oxytocin was secreted by luteal tissue slices at a basal rate of 234·4 ± 32·8 pmol/g per h (n = 24) during 60-min incubations.Activators of protein kinase C: phorbol 12,13-dibutyrate (n = 8), phorbol 12-myristate,13-acetate (n = 4) and 1,2-didecanoylglycerol (n = 5), caused a dose-dependent stimulation of oxytocin secretion in the presence of a calcium ionophore (A23187; 0·2 μmol/l). Phospholipase C (PLC; 50–250 units/l) also caused a dose-dependent stimulation of oxytocin secretion by luteal slices. Phospholipase C-stimulated oxytocin secretion was potentiated by the addition of an inhibitor of diacylglycerol kinase (R59 022; n = 4). These data suggest that the activation of protein kinase C has a role in the stimulation of luteal oxytocin secretion. The results are also consistent with the involvement of protein kinase C in PLC-stimulated oxytocin secretion. The cyclic AMP second messenger system does not appear to be involved in the control of oxytocin secretion by the corpus luteum. Journal of Endocrinology (1990) 124, 225–232


Sign in / Sign up

Export Citation Format

Share Document