scholarly journals Apolipoprotein E gene determines serum testosterone and dehydroepiandrosterone levels in postmenopausal women

2002 ◽  
pp. 503-506 ◽  
Author(s):  
I Zofkova ◽  
K Zajickova ◽  
M Hill ◽  
A Horinek
Keyword(s):  
Apolipoprotein E ◽  
Apoe Genotype ◽  
Multiple Comparisons ◽  
Serum Testosterone ◽  
Dose Effect ◽  
Genotype Distribution ◽  
Testosterone Levels ◽  
Significant Difference ◽  
E4 Allele ◽  
Allele Combination

OBJECTIVE: Apolipoprotein E (ApoE) is believed to play an important role in lipid metabolism and has been found to be related to diseases associated with ageing, the important characteristic of which is decline in circulating sex steroids, including androgen. DESIGN: To find the relationships of levels of serum testosterone and its precursor, dehydroepiandrosterone (DHEA), to ApoE polymorphism in 113 postmenopausal Caucasian women. METHODS: The ApoE genotype was assessed by polymerase chain reaction and CfoI endonuclease digestion. ApoE genotype distribution was as follows: E2/3, 15%; E3/3, 71.7%; E2/4, 1.8%; E3/4, 10.6; and E4/4, 0.89%. The differences in serum androgen levels between genotypes were evaluated by ANCOVA and least significant difference (LSD) multiple comparisons test after adjustment for body mass index, age and/or years since menopause. RESULTS: Significant intergroup differences between the most frequent allele combination (2/3, 3/3 and 3/4) in serum DHEA levels were found (P<0.05, ANCOVA). DHEA levels were higher in women with the E3/4 allele combination than in the E3/3 genotype (P<0.01, LSD multiple comparisons). In serum testosterone levels, borderline intergroup differences were found (P<0.07, ANCOVA). Higher testosterone levels were found in the E3/4 allele combination as compared with E3/3 (P<0.05, LSD multiple comparisons). Dose effect of E4 allele analysis indicated higher serum DHEA and testosterone levels in women with the E4 allele present than in women with the E4 allele absent (P<0.003 for DHEA, P<0.007 for testosterone, ANCOVA). CONCLUSIONS: Circulating testosterone and DHEA are associated with the ApoE genotype, which may render women carrying the allele E4 more susceptible to the development of some diseases associated with ageing and menopause [corrected].

scholarly journals Apolipoprotein e gene polymorphism as a risk factor for ischemic cerebrovascular disease

2004 ◽  
Vol 23 (3) ◽  
pp. 255-264 ◽  
Author(s):  
Sanja Stankovic ◽  
Zagorka Jovanovic-Markovic ◽  
Nada Majkic-Singh ◽  
Aleksandra Stankovic ◽  
Sanja Glisic ◽  
...  
Keyword(s):  
Risk Factor ◽  
Gene Polymorphism ◽  
Cerebrovascular Disease ◽  
Apolipoprotein E ◽  
Apoe Genotype ◽  
Control Group ◽  
Ischemic Cerebrovascular Disease ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
E4 Allele

The possible association of apolipoprotein E (apoE) DNA polymorphism with ischemic cerebrovascular disease was evaluated in 65 patients who had suffered completed stroke or transient ischemic attack and 330 healthy controls. ApoE genotypes were determined by restriction isotyping/MADGE analysis. Significant difference in apoE genotype frequencies between case and control group was observed (p<0.01). Patients affected by ischemic stroke had higher frequency of E4 allele and lower E2 allele than age-matched control subjects. Compared with persons without E4 allele, carriers of an E4 allele had 2.1 times higher risk of incident stroke. Our results indicate that the apoE gene polymorphism may be a risk factor for the development of ischemic cerebrovascular disease in Serbian population..

scholarly journals Effect of short-term beta-hydroxy-beta-methylbutyrate (HMB) supplementation on serum cortisol and testosterone levels in male wrestlers following an exhaustive exercise

2020 ◽  
Vol 42 (5) ◽  
pp. 572-580
Author(s):  
Davoud Tavangar ◽  
Abbas Sadegi ◽  
Hassan Pourrazi
Keyword(s):  
Resting State ◽  
Serum Testosterone ◽  
Serum Cortisol ◽  
Exhaustive Exercise ◽  
Short Term ◽  
Testosterone Levels ◽  
Exhausting Exercise ◽  
Significant Difference ◽  
Before And After ◽  
Methyl Butyrate

Background: The use of HMB supplement is prevalence among athletes to reduce proteolysis and increase muscle mass. Therefore, the purpose of this study was to investigate the effect of short-term beta-hydroxy-beta-methylbutyrate (HMB) supplementation on serum cortisol and testosterone levels in wrestling men following an exhaustive exercise. Methods: Sixteen male wrestlers were randomly divided into two groups including: HMB supplementation (40 mg/kg body weight; n=8) and placebo (n=8). Both groups, after two weeks of supplementation, performed an exhaustive exercise. Blood was obtained before supplementation (Base), before exhaustive exercise, immediately after exhaustive exercise, one hour and 24 hours’ after exhaustive exercise. Circulating concentrations of cortisol and testosterone were assayed. Results: The results of this study indicated that there was no significant difference between the two groups at serum cortisol levels of resting state (before exhaustive exercise) and after exhaustive exercise (immediately, one hour and 24 hours later) (p<0.05). However, there was significant difference between the HMB and placebo groups at serum testosterone levels and testosterone/cortisol of resting state (before exhaustive exercise) and after exhaustive exercise (p<0.05). Conclusion: In general, short-term beta-hydroxy beta-methyl butyrate (HMB) supplementation does not affect the serum cortisol in male wrestlers before and after an exhausting exercise. However, HMB supplementation can lead to a significant increase in serum testosterone and T/C before and after an exhausting exercise.

scholarly journals Distribution of apolipoprotein E gene polymorphism in students and in high-educated elderly from Serbia

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 865-872
Author(s):  
Nela Maksimovic ◽  
Ivana Novakovic ◽  
Vesna Ralic ◽  
Elka Stefanova
Keyword(s):  
Gene Polymorphism ◽  
Apolipoprotein E ◽  
Apoe Genotype ◽  
Human Populations ◽  
Nucleotide Polymorphisms ◽  
Gene Encoding ◽  
Genetic Determination ◽  
Asian Populations ◽  
Significant Difference ◽  
Older Populations

Apolipoprotein E (ApoE) play important role in lipid metabolism and in processes of remodeling and reparation in central nervous system. Three common ApoE isoforms, ApoE2, ApoE3 and ApoE4, show strong genetic determination by ?2, ?3, and ?4 allele. In human genome gene encoding Apolipoprotein E (APOE) is located on cromosome 19, and ?2/?3/?4 haplotype system is defined by 2 non-synonymous single nucleotide polymorphisms (SNPs) in the APOE exon 4. The frequency of the three APOE alleles and corresponding genotypes varies across human populations, with possible clinical implications. At least, variable distribution of ?4 allele may contribute to the regional risk of cardiovascular and Alzheimer?s diseases. Allele-frequency comparisons between younger and older populations suggest an effect of APOE on mortality, but these data are not consistently confirmed. In the present study we have analyzed the distribution of APOE gene polymorphism in a group of University students and retained University professors living in Serbia. After DNA extraction from peripheral blood samples, the APOE genotype was determined by polymerase chain reaction (PCR) followed with HhaI restriction digestion. We found no statistically significant difference in alleles and genotypes distribution between younger and elder group of participants. Also, there was no significant difference compared to APOE data previously obtained in YUSAD cohort of healthy school children (15 y of age) from different regions of Serbia. In both of our groups, as well as in YUSAD cohort, frequency of APOE ?4 allele was <10%. The observed frequencies are lower than in neighboring countries, but similar with Spanish data and some Asian populations. Our results do not support important role of APOE ?4 in the morbidity and mortality in Serbian population, but gene-environmental-social interactions should be considered.

Proposed Relation of Testosterone Levels to Male Suicides and Sudden Deaths

1986 ◽  
Vol 59 (1) ◽  
pp. 100-102 ◽  
Author(s):  
Billy C. Roland ◽  
James L. Morris
Keyword(s):  
Sudden Death ◽  
Serum Testosterone ◽  
Testosterone Levels ◽  
Significant Difference

Samples of serum testosterone were taken from male cadavers (39 suicide and 48 sudden death). There was a significant difference in mean testosterone levels between modes of death for ages ranging from 23 to 45 yr.

ACE I/D polymorphism in Alzheimer’s disease

2008 ◽  
Vol 3 (1) ◽  
pp. 49-54
Author(s):  
Marianna Trebunova ◽  
Eva Slaba ◽  
Viera Habalova ◽  
Zuzana Gdovinova
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Interaction Analysis ◽  
Neurological Diseases ◽  
Apoe Genotype ◽  
Gene Interaction ◽  
Genotype Distribution ◽  
Ace Gene ◽  
Increased Risk ◽  
E4 Allele

AbstractAngiotensin-converting enzyme (ACE) has been reported to show altered activity in patients with neurological diseases. The recent studies found that a 287 bp insertion/deletion (I/D) polymorphism of the ACE gene may be associated with susceptibility to Alzheimer’s disease (AD) but the results have been heterogenous between studies in Europe. In the present study we examined for the first time the association of ACE I/D polymorphism along with APOE genotype in 70 sporadic AD and 126 control subjects in Slovak Caucasians (Central Europe). An increased risk for AD was observed in subjects with at least one APOE*E4 allele (OR=3.99, 95% CI=1.97–8.08). No significant differences for the genotype distribution or the allele frequency were revealed comparing controls and patients for ACE gene. Gene-gene interaction analysis showed increase of the risk to develop AD in subjects carrying both the ACE DD genotype and the APOE*E4 allele (OR=10.32, 95% C.I. 2.67–39.81).

scholarly journals Difference in apolipoprotein E genotype distribution between dentate and edentulous elderly patients with Alzheimer disease

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 699-706
Author(s):  
Aleksandra Popovac ◽  
Ivica Stancic ◽  
Nebojsa Despotovic ◽  
Nadja Nikolic ◽  
Elka Stefanova ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Elderly Patients ◽  
Apolipoprotein E ◽  
Tooth Loss ◽  
Dental Status ◽  
Genotype Distribution ◽  
Buccal Swabs ◽  
Pcr Rflp ◽  
E4 Allele ◽  
Apolipoprotein E Genotype

Association between dementia and tooth loss has been shown although the nature of that association is not clear. It has also been shown that risk of dementia was increased in apolipoprotein E4 allele carriers. The objective of this study was to determine the frequency of APOE alleles and their association with the dental status in elderly demented patients. Dental status of 67 patients with dementia was recorded. DNA was isolated from buccal swabs and genotyping was done by PCR-RFLP. The majority of participants had E3/E4 genotype (55.2%) and these heterozygotes were significantly more frequent than any other genotype (p<0.001). There was no significant association between dental status and genotype. However, partial edentulousness with very few teeth in both jaws (1-9 teeth) was significantly more frequent among demented patients with E3/E4 genotype (p=0.021). Patients with Alzheimer disease most frequently had E3/E4 genotype and had very few or no teeth.

No relationship between testosterone levels and depressive symptoms in aging men

2002 ◽  
Vol 17 (7) ◽  
pp. 411-413 ◽  
Author(s):  
Yasuhiro Kaneda ◽  
Akira Fujii
Keyword(s):  
Depressive Symptoms ◽  
Serum Testosterone ◽  
The Elderly ◽  
Testosterone Levels ◽  
Aging Men ◽  
Significant Difference ◽  
The Mean

SummaryWe investigated the associations between depression and serum testosterone levels in the elderly. There was no significant difference in the mean levels of the serum testosterone between the groups of patients and normals. For both groups, there was no significant correlation between testosterone and total depression scores or age.

Polymorphisms of apolipoprotein E; outcome and susceptibility in multiple sclerosis

2000 ◽  
Vol 6 (1) ◽  
pp. 32-36 ◽  
Author(s):  
S JM Weatherby ◽  
C LA Mann ◽  
M B Davies ◽  
D Carthy ◽  
A A Fryer ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Apolipoprotein E ◽  
Disease Duration ◽  
Age Of Onset ◽  
Neurological Diseases ◽  
Age At Onset ◽  
Apoe Genotype ◽  
Disease Process ◽  
Genotype Frequencies ◽  
Significant Difference

Allelic variants of the apolipoprotein E (APOE) gene influence the course of several neurological diseases. In multiple sclerosis the concentration of APOE in cerebrospinal fluid and its intrathecal synthesis is reduced. Specific isoforms of APOE may also be important and it has been suggested that possession of the e4 allele may be associated with a more aggressive disease process. These data prompted us to re-examine, in a large group of patients with multiple sclerosis, the proposal that allelism in the apolipoprotein gene influences disease course. Genotypes were determined in a well-defined group of 370 unrelated Caucasians with clinically definite multiple sclerosis and in 159 healthy controls. Age at onset, sex, disease duration, disease subtype were recorded. Disability was measured using the Kurtzke expanded disability status score in patients with a disease duration of 10 years or greater. There was no significant difference in APOE allele or genotype frequencies between patients and controls, between disease subtypes or between genders. APOE genotype did not significantly influence age of onset, and no significant relationship between genotype, allele frequency and disease severity was found. This study suggests that individual APOE alleles or genotypes do not determine disease susceptibility or the clinical course of multiple sclerosis.

scholarly journals Comparison of Basal Serum Testosterone Levels between Male Athletes and Martial Artists

2018 ◽  
Vol 6 (11) ◽  
Author(s):  
Roberto Arjona-Luna
Keyword(s):  
Martial Arts ◽  
Serum Testosterone ◽  
Total Serum ◽  
Control Group ◽  
Total Testosterone ◽  
Male Athletes ◽  
Testosterone Levels ◽  
Biological Stress ◽  
Significant Difference ◽  
The Relationship

There are reports about the relationship between testosterone levels and aggressiveness in animals and humans. The practice of martial arts requires high levels of pain tolerance, fear control and of course, enough aggressiveness to overcome psychological and biological stress. Therefore, the objective of the present study was to identify the relationship between basal testosterone levels and martial arts practice by comparing total serum testosterone values between male athletes and martial artists. In this cross-sectional study, the total testosterone between a control group of 15 male athletes and 15 male martial artists was compared. The participants had the following inclusion criteria: healthy men between 18 and 35 years old, not obese, with at least 1 year of continuous training, 3 to 5 sessions per week with a duration from 60 to 90 min at a moderate to high intensity, non smokers, alcohol free, and free from exogenous testosterone or testosterone precursors. Blood samples were recolected between 8:00 to 10:00 am and the laboratory results were obtained by chemiluminescence. The testosterone levels mean of the martial artist's group was 6.44 (±1.17) ng/mL and the athlete's control group had a mean of 6.09 (±1.32) ng/mL. Comparing values with the Student´s t-test showed no statistically significant difference, with a p value of 0.45. There is no significant difference of basal total testosterone levels between male martial artists and athletes, and it seems there is no direct relationship between testosterone levels and martial arts practice. Further investigation on the physiologic responses produced by the practice of combat sports is a growing necessity.

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