Steroid hormones regulate sperm–oviduct interactions in the bovine

After insemination in the cow, a sperm reservoir is formed within the oviducts, allowing the storage and then progressive release of spermatozoa toward the ovulated oocyte. In order to investigate the hormonal regulation of these events in vitro, the ovarian steroids 17β-estradiol (E2) and progesterone (P4) were added at various concentrations to monolayers of bovine oviduct epithelial cells (BOEC) before or during co-incubation with spermatozoa. Main findings demonstrate that (1) a 18-h pretreatment of BOEC with 100 pg/mL and 100 ng/mL of E2 decreased by 25% the ability of BOEC to bind spermatozoa after 10 min, and for the highest dose of E2, 60 min of co-incubation; (2) P4 at concentrations of 10, 100 and 1000 ng/mL induced the release within 60 min of 32–47% of bound spermatozoa from BOEC; this sperm-releasing effect was maintained after a 18-h pretreatment of BOEC with 100 pg/mL of E2; (3) E2 in concentrations above 100 pg/mL inhibited the releasing effect of P4 on bound sperm in a dose-dependent manner; (4) spermatozoa bound to BOEC, then released from BOEC by the action of P4-induced higher cleavage and blastocyst rates after in vitro fertilization than the control group. These results support the hypothesis that the dynamic changes in steroid hormones around the time of ovulation regulate the formation of the sperm reservoir and the timed delivery of capacitated spermatozoa to the site of fertilization.

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Ellagitannins – Compounds From Pomegranate as Possible Effector in Steroidogenesis of Rabbit Ovaries

Physiological Research ◽

10.33549/physiolres.932971 ◽

2015 ◽

pp. 583-585 ◽

Cited By ~ 1

Author(s):

D. PACKOVA ◽

A. A. CARBONELL-BARRACHINA ◽

A. KOLESAROVA

Keyword(s):

Steroid Hormones ◽

Mature Female ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Progesterone Secretion ◽

New Zealand White Rabbits ◽

17Β Estradiol ◽

Dose Dependent ◽

Sexually Mature

This study has observed possible effect of ellagitannins – compounds from pomegranate on process of steroidogenesis in ovaries. The aim of the study was to investigate the possible effect of punicalagin on secretion of steroid hormones – progesterone, androstenedione, testosterone and 17β-estradiol by ovarian fragments of rabbits in vitro. Ovarian fragments from sexually mature female New Zealand white rabbits (n=20) were incubated without (control group) or with punicalagin at various doses 1, 10 and 100 μg.ml−1 for 24 h. Hormones were evaluated by ELISA (The Enzyme-Linked Immunosorbent Assay). Data showed that progesterone and 17β-estradiol (but not androstenedione and testosterone) release by rabbit ovarian fragments was significantly affected by punicalagin addition at various doses. Punicalagin (at 100 μg.ml−1) significantly (P<0.05) increased progesterone secretion. On the other hand, the release of 17β-estradiol was significantly (P<0.005) decreased by punicalagin addition (at 10 μg.ml−1). Our results suggest that punicalagin could have dose-dependent impact on secretion of steroid hormones progesterone and 17β-estradiol by rabbit ovarian fragments and it may be effector in process of ovarian steroidogenesis.

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Effect of Synchronous Versus Sequential Regimens on the Pharmacokinetics and Biodistribution of Regorafenib with Irradiation

Pharmaceutics ◽

10.3390/pharmaceutics13030386 ◽

2021 ◽

Vol 13 (3) ◽

pp. 386

Author(s):

Tung-Hu Tsai ◽

Yu-Jen Chen ◽

Li-Ying Wang ◽

Chen-Hsi Hsieh

Keyword(s):

Stereotactic Body Radiation Therapy ◽

In Vivo Studies ◽

Control Group ◽

High Dose ◽

Dependent Manner ◽

Hep G2 ◽

Time Curve ◽

Dose Dependent

This study was performed to evaluate the interaction between conventional or high-dose radiotherapy (RT) and the pharmacokinetics (PK) of regorafenib in concurrent or sequential regimens for the treatment of hepatocellular carcinoma. Concurrent and sequential in vitro and in vivo studies of irradiation and regorafenib were designed. The interactions of RT and regorafenib in vitro were examined in the human hepatoma Huh-7, HA22T and Hep G2 cell lines. The RT–PK phenomenon and biodistribution of regorafenib under RT were confirmed in a free-moving rat model. Regorafenib inhibited the viability of Huh-7 cells in a dose-dependent manner. Apoptosis in Huh-7 cells was enhanced by RT followed by regorafenib treatment. In the concurrent regimen, RT decreased the area under the concentration versus time curve (AUC)regorafenib by 74% (p = 0.001) in the RT2 Gy × 3 fraction (f’x) group and by 69% (p = 0.001) in the RT9 Gy × 3 f’x group. The AUCregorafenib was increased by 182.8% (p = 0.011) in the sequential RT2Gy × 1 f’x group and by 213.2% (p = 0.016) in the sequential RT9Gy × 1 f’x group. Both concurrent regimens, RT2Gy × 3 f’x and RT9Gy × 3 f’x, clearly decreased the biodistribution of regorafenib in the heart, liver, lung, spleen and kidneys, compared to the control (regorafenib × 3 d) group. The concurrent regimens, both RT2Gy × 3 f’x and RT9Gy × 3 f’x, significantly decreased the biodistribution of regorafenib, compared with the control group. The PK of regorafenib can be modulated both by off-target irradiation and stereotactic body radiation therapy (SBRT).

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Protein-free phospholipid emulsion treatment improved cardiopulmonary function and survival in porcine sepsis

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00285.2002 ◽

2003 ◽

Vol 284 (2) ◽

pp. R550-R557 ◽

Cited By ~ 22

Author(s):

Roy D. Goldfarb ◽

Thomas S. Parker ◽

Daniel M. Levine ◽

Dana Glock ◽

Imran Akhter ◽

...

Keyword(s):

Density Lipoprotein ◽

Fibrin Clot ◽

Control Group ◽

High Dose ◽

Dependent Manner ◽

Serum Lipoproteins ◽

Treatment Groups ◽

Tnf Α ◽

Dose Dependent

Lipoprotein phospholipid (PL) plays a major role in neutralization of endotoxin. This study tested the hypothesis that prophylactic administration of a PL-enriched emulsion (PRE), which augments PL content of serum lipoproteins and neutralizes endotoxin in vitro, would preserve cardiovascular function and improve survival in porcine septic peritonitis. A control group was compared with low-, mid-, and high-dose treatment groups that received PRE by primed continuous infusion for 48 h. A fibrin clot containing live Escherichia coli 0111.B4 was implanted intraperitoneally 30 min after the priming dose. Survival increased in a dose-dependent manner and was correlated with serum PL. Infused PL was associated with high-density lipoprotein in the low-dose group and all serum lipoproteins at higher doses. Treatment significantly lowered serum endotoxin and tumor necrosis factor (TNF)-α, preserved cardiac output and ejection fraction, and attenuated increases in systemic and pulmonary vascular resistances. This study demonstrated that augmentation of lipoprotein PL via administration of PRE improved survival and offered a novel therapeutic approach to sepsis.

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Hormonal regulation of PGE2 and COX-2 production in rabbit uterine cervical fibroblasts

Journal of Applied Physiology ◽

10.1152/jappl.2001.90.4.1227 ◽

2001 ◽

Vol 90 (4) ◽

pp. 1227-1231 ◽

Cited By ~ 10

Author(s):

T. Sato ◽

H. Michizu ◽

K. Hashizume ◽

A. Ito

Keyword(s):

Hormonal Regulation ◽

Uterine Contraction ◽

Dependent Manner ◽

Physiological Concentration ◽

17Β Estradiol ◽

Interleukin 1Α ◽

Cox 2 ◽

Constitutive Production ◽

Dose Dependent ◽

Cox 1

Prostaglandins (PGs) cause uterine contraction to initiate labor at term. We investigated the effect of progesterone and 17β-estradiol on the production of PGE2in rabbit uterine cervical fibroblasts. When the cervical fibroblasts were treated with interleukin-1α (IL-1α), the level of PGE2 was augmented in a time- and dose-dependent manner. The IL-1α-augmented PGE2 level was almost completely suppressed by progesterone and 17β-estradiol at the physiological concentration (0.01 μM), whereas a slight decrease in the basal level of PGE2 was observed in the cervical fibroblasts treated with both hormones at a pharmacological concentration (1 μM). In addition, the level of PGE2 augmented by IL-1α was due to the increase of cyclooxygenase (COX) activity, which was inhibited by progesterone and 17β-estradiol as well as by indomethacin and a specific COX-2 inhibitor, NS-398, but not by the well-known COX-1 inhibitor, aspirin. Furthermore, progesterone and 17β-estradiol suppressed the IL-1α-augmented COX-2 production but not the constitutive production of COX-1 in rabbit uterine cervical fibroblasts. These results suggest that progesterone and 17β-estradiol prevent the initiation of labor by inhibiting PGE2 production after the suppression of COX-2 production during pregnancy in the rabbit.

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Proliferation Inhibition and Apoptosis Induction of Juglone on Human Cervical Cancer HeLa Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.912-914.1961 ◽

2014 ◽

Vol 912-914 ◽

pp. 1961-1964 ◽

Cited By ~ 1

Author(s):

Wei Zhang ◽

Wen He Zhu ◽

Yan Li ◽

Jun Luo ◽

Shi Jie Lv

Keyword(s):

Cervical Cancer ◽

Hela Cells ◽

Control Group ◽

Dependent Manner ◽

Inverted Microscope ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Dose Dependent ◽

Human Cervical Cancer

Abstract: To investigate whether juglone could inhibits the proliferation on human cervical cancer cells (HeLa) in vitro. Cells were divided into control group, different concentration (10μM, 20μM, 50μM, 100μM and200μM) juglone groups for different durations. The viability of HeLa cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The morphology changes of HeLa cells were observed by inverted microscope .The results showed that the viability of HeLa cells was decreased and the cell morphology was changed in a dose-dependent manner after treatment different concentration juglone for 24h when compared with control group. The results suggest that Juglone may be effective for the treatment of HeLa cells.

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Roles of oxidative stress, apoptosis, and heme oxygenase-1 in ethylbenzene-induced renal toxicity in NRK-52E cells

Toxicology and Industrial Health ◽

10.1177/0748233715602834 ◽

2016 ◽

Vol 32 (12) ◽

pp. 1952-1960

Author(s):

Ming Zhang ◽

Yanrang Wang ◽

Xiaojun Wang ◽

Jing Liu ◽

Jingshu Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Heme Oxygenase ◽

Messenger Rna ◽

Renal Toxicity ◽

Control Group ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Dependent Manner ◽

Dose Dependent

Ethylbenzene is an important industrial chemical, but its potential toxicity is a recent concern. Our previous study investigated the renal toxicity of ethylbenzene in vivo. Rat renal epithelial cells (NRK-52E cells) were incubated with 0, 30, 60, and 90 µmol/L of ethylbenzene for 24 h in vitro to investigate ethylbenzene-induced oxidative stress, apoptosis, and the expression of heme oxygenase 1 (HO-1) and nuclear factor (erythroid 2)-related factor 2 (Nrf2). The cell survival rate in the ethylbenzene-treated groups was significantly lower than the control group. Ethylbenzene significantly increased intracellular reactive oxygen species and apoptosis. Malondialdehyde levels were significantly elevated compared with the control group, while glutathione levels and glutathione peroxidase activities were decreased in ethylbenzene-treated groups. The activities of catalase and superoxide dismutase were also markedly reduced. A significant dose-dependent increase in HO-1 and Nrf2 messenger RNA expression levels was observed in ethylbenzene-treated groups compared with the control group. Similarly, ethylbenzene treatment enhanced protein expression of HO-1 and Nrf2 in a dose-dependent manner. Our results indicated that ethylbenzene induced oxidative stress, apoptosis, and upregulation of HO-1 and Nrf2 in NRK-52E cells, which contributes to ethylbenzene-induced renal toxicity.

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AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway

Journal of Diabetes Research ◽

10.1155/2018/6823058 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Ying-Hua Chen ◽

Zhang-Wei Chen ◽

Hong-Mei Li ◽

Xin-Feng Yan ◽

Bo Feng

Keyword(s):

Umbilical Vein ◽

Underlying Mechanism ◽

Control Group ◽

Dependent Manner ◽

Antioxidant Treatment ◽

Glycation End Products ◽

Ros Scavenger ◽

Umbilical Vein Endothelial Cells ◽

Dose Dependent

Objective. Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism. Methods. In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400 μg/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger. The number of CD31-positive EMPs was assessed by flow cytometry. Results. The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner. In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group. These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody. Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs. Conclusion. Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS.

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The role of vaspin in porcine corpus luteum

Journal of Endocrinology ◽

10.1530/joe-20-0332 ◽

2020 ◽

Vol 247 (3) ◽

pp. 283-294 ◽

Cited By ~ 2

Author(s):

Patrycja Kurowska ◽

Ewa Mlyczyńska ◽

Monika Dawid ◽

Małgorzata Grzesiak ◽

Joelle Dupont ◽

...

Keyword(s):

Corpus Luteum ◽

Hormonal Regulation ◽

Luteal Phase ◽

Dependent Manner ◽

Luteal Cells ◽

Visceral Adipose ◽

Dose Dependent ◽

Late Stages

Vaspin, visceral adipose tissue-derived serine protease inhibitor, plays important roles in inflammation, obesity, and glucose metabolism. Our recent research has shown the expression and role of vaspin in the function of ovarian follicles. However, whether vaspin regulates steroidogenesis and luteolysis in the corpus luteum (CL) is still unknown. The aim of this study was first to determine the expression of vaspin and its receptor GRP78 in porcine CL at the early, middle, and late stages of the luteal phase. Next, we investigated the hormonal regulation of vaspin levels in luteal cells in response to luteinizing hormone (LH), progesterone (P4), and prostaglandin PGE2 and PGF2α. Finally, we determined vaspin’s direct impact on luteal cells steroidogenesis, luteolysis and kinases phosphorylation. Our results are the first to show higher vaspin/GRP78 expression in middle and late vs early stages; immunohistochemistry showed cytoplasmic vaspin/GRP78 localization in small and large luteal cells. In vitro, we found that LH, P4, PGE2, and PGF2α significantly decreased vaspin levels. Furthermore, vaspin stimulated steroidogenesis by the activation of the GRP78 receptor and protein kinase A (PKA). Also, vaspin increased the ratio of luteotropic PGE2 to luteolytic PGF2α secretion via GRP78 and mitogen-activated kinase (MAP3/1). Moreover, vaspin, in a dose-dependent manner, decreased GRP78 expression, while it, in a time-dependent manner, increased kinases PKA and MAPK3/1 phosphorylation. Taken together, we found that vaspin/GRP78 expression depends on the luteal phase stage and vaspin affects luteal cells endocrinology, indicating that vaspin is a new regulator of luteal cells steroidogenesis and CL formation.

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Phenolic Compounds from Water-Ethanol Extracts ofTetrapleura tetrapteraProduced in Cameroon, as Potential Protectors againstIn VivoCCl4-Induced Liver Injuries

The Scientific World JOURNAL ◽

10.1155/2019/5236851 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Peter William Kemewele Saague ◽

Bruno Moukette Moukette ◽

Jacques Romain Njimou ◽

Prosper Cabral Nya Biapa ◽

Francine Nzufo Tankeu ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Hepatoprotective Activity ◽

Control Group ◽

Dependent Manner ◽

Polyphenolic Extract ◽

Tetrapleura Tetraptera ◽

Dose Dependent

Background. Liver diseases are a global health problem. Medicinal plants are being increasingly used to manage a wide variety of diseases including liver disorders. The aim of this study was to investigate the antioxidant properties and hepatoprotective activity of polyphenolic extract from the fruits ofTetrapleura tetraptera (T. tetraptera).Results. The extract ofT. tetrapterawas administered at doses of 50 mg/kg and 100 mg/kg for 07per osto rats before the induction of hepatotoxicity with of 2 ml/kg of 1:1 (v/v) carbon tetrachloride (CCl4) and olive oil through intraperitoneal route. Thein vitroantioxidant and radical scavenging properties ofT. tetrapterawere conducted by the FRAP method, the phosphomolybdate method, and the inhibition potential of DPPH, ABTS, OH, and NO radicals. The extraction yield ofT. tetrapterawas 19.35%. This extract contains polyphenols (273.48 mg CAE/g DM), flavonoids (5.2549 mg SE/g DM), and flavonols (1.615 mg SE/g DM). This extract showedin vitroantioxidant activity, an inhibitor power of various free radicals, and radical scavenging potential dose-dependent. The fifty-percent inhibitory concentration of the extract (IC50) for the studied radical varied from 28.16 to 136μg/L. In rats treated with the extract ofT. tetraptera, in a dose-dependent manner, the levels of hepatotoxicity markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) significantly increased while the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and the level of reduced glutathione (GHS) significantly increased compared to the control group.Conclusions. The extracts from the fruit ofT. tetrapterademonstrate antioxidant activity and hepatoprotective effects.

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Purified Astaxanthin from Haematococcus pluvialis Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen In Vitro and In Vivo

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/4946902 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Hsin-Yu Chou ◽

Dik-Lung Ma ◽

Chung-Hang Leung ◽

Chien-Chih Chiu ◽

Tzyh-Chyuan Hour ◽

...

Keyword(s):

Oxidative Stress ◽

Haematococcus Pluvialis ◽

Tissue Injury ◽

Fluorescein Isothiocyanate ◽

Normal Fibroblast ◽

Control Group ◽

Dependent Manner ◽

Dose Dependent

Intracellular reactive apoptosis and reactive oxygen species (ROS) play a crucial role in ultraviolet- (UV-) induced inflammation and aging reaction in human dermal tissues. This study determines the mechanism by which Haematococcus pluvialis extracts (HPE) and purified astaxanthin (HPA) to promote skin regeneration in the injured tissue in vitro and in vivo. The results show that HPE and HPA decrease the DNA damage and promote the secretion of collagen from the human normal fibroblast cell line (Hs68) in a dose-dependent manner. UV irradiation and HPA reduce oxidative stress damage due to phorbol-12-myristate-13-acetate (PMA). When skin cells are injured by free radicals, cells undergo a programmed cellular death. Cellular apoptotic death is determined using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining to verify that there is no cell membrane asymmetry and that the nuclear membrane is broken. Inflammatory symptoms and apoptotic injuries to experimental rats in a group that is treated with HPA treated are decreased in a dose-dependent manner after UVB exposure (300 mJ/cm2) for 15 min in vivo, compared to the vehicle control group. These positive results show that HPA repairs UVB-triggered skin tissue injury and aging by conducting electrons out of cells to maintain a low level of oxidative stress so that collagen is synthesized in vitro and in vivo.

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