scholarly journals GENOME EDITING: CURRENT STATE AND PROSPECTS FOR USE IN POULTRY (review)

2021 ◽  
Vol 56 (6) ◽  
pp. 1015-1030
Author(s):  
N.A. Volkova ◽  
Keyword(s):  
Genome Editing ◽  
Current State

TALEN-mediated genome editing: prospects and perspectives

2014 ◽  
Vol 462 (1) ◽  
pp. 15-24 ◽  
Author(s):  
David A. Wright ◽  
Ting Li ◽  
Bing Yang ◽  
Martin H. Spalding
Keyword(s):  
Genome Editing ◽  
Double Strand Breaks ◽  
Zinc Finger Nucleases ◽  
Transcription Activator ◽  
Strand Breaks ◽  
Natural Processes ◽  
Current State ◽  
A Genome ◽  
Dna Dsbs ◽  
Repair Mechanisms

Genome editing is the practice of making predetermined and precise changes to a genome by controlling the location of DNA DSBs (double-strand breaks) and manipulating the cell's repair mechanisms. This technology results from harnessing natural processes that have taken decades and multiple lines of inquiry to understand. Through many false starts and iterative technology advances, the goal of genome editing is just now falling under the control of human hands as a routine and broadly applicable method. The present review attempts to define the technique and capture the discovery process while following its evolution from meganucleases and zinc finger nucleases to the current state of the art: TALEN (transcription-activator-like effector nuclease) technology. We also discuss factors that influence success, technical challenges and future prospects of this quickly evolving area of study and application.

scholarly journals Genome-Editing and Biomedical Cell Products: Current State, Safety and Efficacy

2018 ◽  
Vol 18 (3) ◽  
pp. 140-149 ◽  
Author(s):  
A. A. Goryaev ◽  
M. V. Savkina ◽  
K. M. Mefed ◽  
V. P. Bondarev ◽  
V. A. Merkulov ◽  
...  
Keyword(s):  
T Cells ◽  
Genome Editing ◽  
Ex Vivo ◽  
Rapid Development ◽  
The United States ◽  
Human Cells ◽  
The European Union ◽  
Antigen Receptors ◽  
Current State ◽  
Cd34 Cells

Advances in ex vivo technologies of human genome editing have made it possible to develop new approaches to the treatment of genetic, oncological, infectious and other diseases, which may involve the use of biomedical cell products. However, despite the rapid development of these technologies and a large number of clinical trials conducted in many countries around the world, only 4 products (Strimvelis, Zalmoxis, Kymriah and Yescarta) containing ex vivo genetically modified human cells are authorised for use in the European Union and the United States of America. This paper considers three promising technologies (ZFN, TALEN and CRISPR) that allow for easy and effective editing of the genome at the sites of interest, thereby creating a platform for further development of the genetic engineering of human cells. It describes the technology of engineering chimeric antigen receptors (CARs). It also provides data on the efficacy and safety of the approved products: Strimvelis which contains autologous CD34+ cells transduced ex vivo with a retroviral vector containing adenosine deaminase gene, Zalmoxis which contains modified allogeneic T-cells, and two products: Kymriah and Yescarta which contain autologous T-cells with CARs to CD19 antigen, intended for the treatment of CD19+ hematological malignancies.

scholarly journals Production of Recombinant Proteins in the Milk of Transgenic Animals: Current State and Prospects

Acta Naturae ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 40-47 ◽  
Author(s):  
M. V. Shepelev ◽  
S. V. Kalinichenko ◽  
A. V. Deykin ◽  
I. V. Korobko
Keyword(s):  
Genetic Engineering ◽  
Genome Editing ◽  
Recombinant Proteins ◽  
Retrospective Review ◽  
Current Trend ◽  
Transgenic Animals ◽  
Current State ◽  
The Creation ◽  
A Current

The use of transgenic animals as bioreactors for the synthesis of the recombinant proteins secreted into milk is a current trend in the development of biotechnologies. Advances in genetic engineering, in particular the emergence of targeted genome editing technologies, have provided new opportunities and significantly improved efficiency in the generation of animals that produce recombinant proteins in milk, including economically important animals. Here, we present a retrospective review of technologies for generating transgenic animals, with emphasis on the creation of animals that produce recombinant proteins in milk. The current state and prospects for the development of this area of biotechnology are discussed in relation to the emergence of novel genome editing technologies. Experimental and practical techniques are briefly discussed.

scholarly journals CRISPRpred(SEQ): a sequence based tool for sgRNA on target activity prediction [(almost) beating Deep Learning pipelines by traditional machine learning]

2019 ◽  
Author(s):  
Ali Haisam Muhammad Rafid ◽  
Md. Toufikuzzaman ◽  
Mohammad Saifur Rahman ◽  
M. Sohel Rahman
Keyword(s):  
Machine Learning ◽  
Deep Learning ◽  
Genome Editing ◽  
Cell Lines ◽  
Genetic Diseases ◽  
Machine Learning Techniques ◽  
Activity Prediction ◽  
Current State ◽  
Learning Techniques ◽  
Target Activity

AbstractAn accurate and fast genome editing tool can be used to treat genetic diseases, modify crops genetically etc. However, a tool that has low accuracy can be risky to use, as incorrect genome editing may have severe consequences. Although many tools have been developed in the past, there are still room for further improvement. In this paper, we present CRISPRpred(SEQ), a sequence based tool for sgRNA on target activity prediction that leverages only traditional machine learning techniques. We compare the results of CRISPRpred(SEQ) with that of DeepCRISPR, the current state-of-the-art, which uses a deep learning pipeline. In spite of using only traditional machine learning methods, we are able to beat DeepCRISPR for the three out of four cell lines in the benchmark dataset convincingly (2.174%, 6.905% and 8.119% improvement for the three cell lines), which is quite outstanding.

scholarly journals Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Alexey S. Kiryushkin ◽  
Elena L. Ilina ◽  
Elizaveta D. Guseva ◽  
Katharina Pawlowski ◽  
Kirill N. Demchenko
Keyword(s):  
Genome Editing ◽  
Hairy Root ◽  
Fluorescent Protein ◽  
Effective Means ◽  
Gene Encoding ◽  
Transgenic Roots ◽  
Translational Enhancer ◽  
Current State ◽  
Root Transformation ◽  
Agrobacterium Rhizogenes Strains

CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy root transformation is a rapid and convenient approach for obtaining transgenic roots. When combined, these techniques represent a fast and effective means of studying gene function. In this review, we outline the current state of the art reached by the combination of these approaches over seven years. Additionally, we discuss the origins of different Agrobacterium rhizogenes strains that are widely used for hairy root transformation; the components of CRISPR/Cas vectors, such as the promoters that drive Cas or gRNA expression, the types of Cas nuclease, and selectable and screenable markers; and the application of CRISPR/Cas genome editing in hairy roots. The modification of the already known vector pKSE401 with the addition of the rice translational enhancer OsMac3 and the gene encoding the fluorescent protein DsRed1 is also described.

Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects

Planta ◽  
2021 ◽  
Vol 255 (1) ◽  
Author(s):  
Dhanawantari L. Singha ◽  
Debajit Das ◽  
Yogita N. Sarki ◽  
Naimisha Chowdhury ◽  
Monica Sharma ◽  
...  
Keyword(s):  
Genome Editing ◽  
Future Prospects ◽  
Tissue Specific ◽  
Current State

Genome Editing: Current State of Research and Application to Animal Husbandry

2019 ◽  
Vol 55 (7) ◽  
pp. 711-721
Author(s):  
N. A. Zinovieva ◽  
N. A. Volkova ◽  
V. A. Bagirov
Keyword(s):  
Genome Editing ◽  
Animal Husbandry ◽  
Current State ◽  
State Of Research

CRISPR/Cas-based genome engineering in natural product discovery

2019 ◽  
Vol 36 (9) ◽  
pp. 1262-1280 ◽  
Author(s):  
Yaojun Tong ◽  
Tilmann Weber ◽  
Sang Yup Lee
Keyword(s):  
Natural Product ◽  
Genome Editing ◽  
Genome Engineering ◽  
State Of The Art ◽  
Natural Product Discovery ◽  
Current State

This review summarizes the current state of the art of CRISPR/Cas-based genome editing technologies for natural product producers.

scholarly journals Genome editing in cereal crops: an overview

2021 ◽  
Author(s):  
Jerlie Mhay Matres ◽  
Julia Hilscher ◽  
Akash Datta ◽  
Victoria Armario-Nájera ◽  
Can Baysal ◽  
...  
Keyword(s):  
Genome Editing ◽  
Crop Improvement ◽  
Agricultural Practices ◽  
Cereal Crops ◽  
Full Potential ◽  
Cereal Crop ◽  
Biotic Stresses ◽  
Current State ◽  
Change Tolerance ◽  
Regulatory Challenges

AbstractGenome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal crops- rice, maize, wheat and barley. Genome editing has been used to achieve important agronomic and quality traits in cereals. These include adaptive traits to mitigate the effects of climate change, tolerance to biotic stresses, higher yields, more optimal plant architecture, improved grain quality and nutritional content, and safer products. Not all traits can be achieved through genome editing, and several technical and regulatory challenges need to be overcome for the technology to realize its full potential. Genome editing, however, has already revolutionized cereal crop improvement and is poised to shape future agricultural practices in conjunction with other breeding innovations.

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