Antioxidant activities of ethanolic extract of Annona muricata against different pro-oxidant induced lipid peroxidation in rat brain and liver

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

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Antidiabetic and Antioxidant Activities of Ethanolic Extract of Dried Flowers of Moringa oleifera in Streptozotocin-induced Diabetic Rats

Acta Facultatis Medicae Naissensis ◽

10.1515/afmnai-2016-0028 ◽

2016 ◽

Vol 33 (4) ◽

pp. 259-272 ◽

Cited By ~ 4

Author(s):

Rotimi Olusanya Arise ◽

Oluwaseun Ruth Aburo ◽

Samuel Tobi Farohunbi ◽

Adenike Adebola Adewale

Keyword(s):

Lipid Peroxidation ◽

Blood Glucose ◽

Antioxidant Activities ◽

Ethanolic Extract ◽

Diabetic Control ◽

Diabetic Rats ◽

The Body ◽

Albino Rats ◽

Reference Drug ◽

Glucose Lowering

Abstract This study was undertaken to determine the antidiabetic and antioxidant effects of oral administration of ethanolic extract of Moringa oleiferaflower on stretozotocin-induced diabetic rats at 100, 200, and 300 mg/kg b.w. Thirty (30) male experimental albino rats were grouped randomly into six groups: groups A, B, and F are the control, diabetic control and reference drug groups, while C-E received 100, 200, and 300mg/kg b.w of the extract, respectively. Blood samples and organs were collected to assay for blood glucose level and antioxidant enzymes. Levels of blood glucose, serum lipids and lipid peroxidation as well as aspartate aminotransferase (AST), alkaline phosphatase (ALP), and alanine aminotransferase (ALT) activities were significantly reduced (p<0.05) in STZ-induced diabetic rats orally administered ethanolic extract of M. oleifera flower. However, the body weight; catalase and superoxide dismutase activities were significantly increased (p < 0.05) when compared with the controls. M. oleifera flower ethanolic extract administered orally therefore exhibited improved lipid metabolism, glucose-lowering potential and is hence beneficial in preventing diabetic complications as a result of lipid peroxidation and oxidative systems in streptozotocin-induced diabetic rats. It could thus be employed therapeutically in managing diabetes mellitus.

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ANTI-INFLAMMATORY ACTIVITY AND PHYTOCHEMISTRY OF EXTRACTS FROM THE ROOT BARK OF CALOTROPIS PROCERA (AIT.) R. BR. HEPATOPROTECTIVE MEDICINAL PLANT FROM BURKINA FASO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i10.39275 ◽

2020 ◽

pp. 185-190

Author(s):

ERNEST NOGMA SOMBIÉ ◽

NOUFOU OUÉDRAOGO ◽

ADAMA HILOU

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Reducing Power ◽

Ethanolic Extract ◽

Calotropis Procera ◽

Root Bark ◽

Dpph Radical ◽

Antioxidant Power ◽

Anti Inflammatory

Objective: This study aimed to assess the anti-inflammatory and antioxidant properties in vitro of Calotropis procera root bark. Methods: Anti-inflammatory activities of the ethanolic extract of root bark of C. procera and its fractions were evaluated on lipoxygenase and xanthine oxidase (XO). Antioxidant activity was evaluated by trapping the 2,2-diphenylpicrylhydrazyl (DPPH) radical, hydrogen peroxide (H2O2), by the reducing power ferric reducing antioxidant power. Inhibitory powers of lipid peroxidation and of degradation of D-deoxyribose as well as the contents of phenolic and triterpene compounds were also evaluated. Results: Ethanolic extract showed interesting anti-inflammatory and antioxidant activities. Butanol fraction which is one of richest fractions for flavonoids (17.38±0.13 mg EQ/10 g extracts) showed the best percentages for inhibitions of XO (60.14±4.89%), degradation of D-deoxyribose (81.04±0.77%), and the DPPH radical (71.43±6.35%). Dichloromethane fraction which is the richest in triterpenes (1067.02±5.77 mg UAE/g extracts) showed the best percentages for inhibitions of lipoxygenase (56.45±1.15%) and of lipid peroxidation (82.45±0.73%). Ethyl acetate and residual aqueous fractions, respectively, showed the best activities of inhibiting H2O2 (75.38±0.76%) and for iron-reducing capacity (23.51±1.69 mg EAA/g extracts). Conclusion: Ethanolic extract from C. procera root bark and its fractions, their particular the butanol and dichloromethane one, has anti-inflammatory and antioxidant properties which could prevent or attenuate liver injury and its complications.

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Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

Myanmar Health Sciences Research Journal ◽

10.34299/mhsrj.00917 ◽

2019 ◽

Vol 30 (3) ◽

pp. 16-22

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Scientific Information ◽

Ethanolic Extract ◽

The Body ◽

World Health ◽

Gas Chromatography Mass Spectrometry ◽

Molecular Formula ◽

Bioactive Components ◽

Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

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Antiulcer and Antioxidant Activity of a Lectin from Mucuna pruriens Seeds on Ethanol- induced Gastropathy: Involvement of Alpha-2 Adrenoceptors and Prostaglandins

Current Pharmaceutical Design ◽

10.2174/1381612825666190524081433 ◽

2019 ◽

Vol 25 (12) ◽

pp. 1430-1439 ◽

Cited By ~ 2

Author(s):

Isabela Ribeiro Pinto ◽

Hellíada V. Chaves ◽

Auriana S. Vasconcelos ◽

Francisca Clea F de Sousa ◽

Tatiane Santi-Gadelha ◽

...

Keyword(s):

Lipid Peroxidation ◽

Acute Toxicity ◽

Antioxidant Activities ◽

Mucuna Pruriens ◽

Toxicity Tests ◽

Sod Activity ◽

Tropical Regions ◽

Mediated Effects ◽

Menstruation Disorders ◽

Therapeutic Tools

Ethnopharmacological Relevance:Mucuna pruriens (Mp) belongs to Leguminosae family, it is native of tropical regions and used to treat several maladies such as urinary, neurological, and menstruation disorders, constipation, edema, fever, tuberculosis, ulcers, diabetes, arthritis, dysentery, and cardiovascular diseases. Mp seeds are rich in bioactive compounds, for instance, lectins, a heterogeneous group of proteins and glycoproteins with a potential role as therapeutic tools for several conditions, including gastric disorders. This study investigated the acute toxicity, gastroprotective, and antioxidant activities of a lectin from Mucuna pruriens seeds (MpLec) on ethanol-induced gastropathy model in mice.Materials & Methods:Mice received MpLec (5 or 10 mg/kg; i.v.) and were observed for acute toxicity signs; in another experimental series, mice were pre-treated with MpLec (0.001; 0.01 or 0.1 mg/kg, i.v.), ranitidine (80 mg/kg, p.o.), or saline (0.3 mL/30g, i.v.) before ethanol 99.9% (0.2 mL/animal, p.o.), and euthanized 30 min after ethanol challenge. Macroscopic and microscopic gastric aspects, biochemical parameters (tissue hemoglobin levels, iron-induced lipid peroxidation, GSH content, SOD activity, and gastric mucosal PGE2) were measured. Additionally, pharmacological tools (yohimbine, indomethacin, naloxone, L-NAME) were opportunely used to clarify MpLec gastroprotective mechanisms of action.Results:No toxicity signs nor death were observed at acute toxicity tests. MpLec reduced ethanol-induced gastric damage, edema, and hemorrhagic patches formation, as well as decreased lipid peroxidation, SOD activity, and increased GSH content. Yohimbine and indomethacin prevented MpLec effects, suggesting the involvement of alpha-2 adrenoceptors and prostaglandins in the MpLec-mediated effects.Conclusion:MpLec does not present toxicity signs and shows gastroprotective and antioxidant activities via alpha-2 adrenoceptors and prostaglandins in the ethanol-induced gastropathy model.

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Management of Dermatophytoses by Foliose Lichen; Heterodermia diademata (Taylor) D.D. Awasthi

Science & Technology Journal ◽

10.22232/stj.2017.05.02.02 ◽

2017 ◽

Vol 5 (2) ◽

pp. 79-84

Author(s):

Anand Pandey ◽

◽

Awadhesh Kumar ◽

Anupam Dikshit ◽

◽

...

Keyword(s):

Fungicidal Activity ◽

Trichophyton Rubrum ◽

Antioxidant Activities ◽

Biological Activities ◽

Ethanolic Extract ◽

Microsporum Gypseum ◽

Superficial Infection ◽

Recent Advancement ◽

Secondary Substances ◽

Foliose Lichen

Lichens are demarcated as an association of two distinct and dissimilar components; the mycobiont (a fungus) and the phycobionts (an alga) forming a composite organism. The recent advancement in science has paid much attention on the biological roles of lichen secondary substances; which are attributed with a lot of biological activities, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-tumor, and antioxidant activities. The current research aims to unravel the fungicidal activity of lichen Heterodermia diademata, ethanolic extract (HDE) against prevalent superficial infection causing dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and M. canis. The HDE was found active against M. gypseum and T. mentagrophytes with MIC; 1.68 and 1.98 mg/ml and mg/ml respectively. The dermatophytes were also analyzed by molecular phylogeny of chs-1 gene for variability in MIC and prediction of susceptible pathogens.

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Strain differences in rat brain and liver σ binding: lack of cytochrome P450-2D1 involvement

European Journal of Pharmacology ◽

10.1016/0014-2999(93)90182-h ◽

1993 ◽

Vol 243 (3) ◽

pp. 249-254 ◽

Cited By ~ 7

Author(s):

Ann Jewell ◽

Peter Wedlund ◽

Linda Dwoskin

Keyword(s):

Cytochrome P450 ◽

Rat Brain ◽

Strain Differences ◽

Rat Brain And Liver

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Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

Scientific Reports ◽

10.1038/s41598-021-83566-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zhichen Cai ◽

Xunhong Liu ◽

Huan Chen ◽

Rong Yang ◽

Jiajia Chen ◽

...

Keyword(s):

Lipid Peroxidation ◽

Salt Stress ◽

Phenolic Acids ◽

Antioxidant Activities ◽

Vital Role ◽

Gray Relational Analysis ◽

Bioactive Constituents ◽

Multivariate Statistical ◽

Medicinal Value ◽

Quality Formation

AbstractLonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, the quality of sessile organisms is dramatically affected by salt stress. To systematically explore the quality formation of LJF in morphology, physiology, and bioactive constituents' response to multiple levels of salt stress, UFLC-QTRAP-MS/MS and multivariate statistical analysis were performed. Lonicera japonica Thunb. was planted in pots and placed in the field, then harvested after 35 days under salt stress. Indexes of growth, photosynthetic pigments, osmolytes, lipid peroxidation, and antioxidant enzymes were identified to evaluate the salt tolerance in LJF under different salt stresses (0, 100, 200, and 300 mM NaCl). Then, the total accumulation and dynamic variation of 47 bioactive constituents were quantitated. Finally, Partial least squares discrimination analysis and gray relational analysis were performed to systematically cluster, distinguish, and evaluate the samples, respectively. The results showed that 100 mM NaCl induced growth, photosynthetic, antioxidant activities, osmolytes, lipid peroxidation, and multiple bioactive constituents in LJF, which possessed the best quality. Additionally, a positive correlation was found between the accumulation of phenolic acids with antioxidant enzyme activity under salt stress, further confirming that phenolic acids could reduce oxidative damage. This study provides insight into the quality formation and valuable information to improve the LJF medicinal value under salt stress.

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