scholarly journals Seed dormancy of Ochradenus baccatus (Resedaceae), a shrubby species from Arabian desert regions

2016 ◽  
Vol 64 (3) ◽  
Author(s):  
Arvind Bhatt ◽  
Félix Pérez-García
Keyword(s):  
Room Temperature ◽  
Water Solution ◽  
Storage Period ◽  
Seed Storage ◽  
Storage Conditions ◽  
Food Sources ◽  
Continuous Darkness ◽  
Physiological Dormancy ◽  
Desert Regions ◽  
One Year

Ochradenus baccatus is a perennial glycophyte growing in Middle East and it is one of the most important food sources for many animal species in desert regions. The aim of our study was to investigate the effects of seed storage, light, temperature and gibberellic acid (GA3) on germination of O. baccatus seeds. We also investigated the germination characteristics of O. baccatus seeds under different saline concentration and their capability to recover germination once they were transferred to distilled water. Seeds were stored at room temperature (20 ± 2 ºC) and at -18 ºC. Germination tests were conducted at alternating temperatures of 15/25, 20/30 and 25/35 ºC in either continuous darkness or photoperiod of 12-h dark/12-h light. To study the effect of GA3 on germination of O. baccatus seeds, freshly-collected seeds and stored seeds were soaked for 24 h in a GA3 water solution (1 g/L) before sowing. To assess the salinity tolerance during germination, seeds were germinated under different salinity levels (100, 200 and 400 mM NaCl). Stored seeds at room temperature and -18 ºC germinate equally well at different temperature regimes and light conditions. However, freshly matured seeds were not able to germinate even when they were treated with GA3. On the contrary, stored seeds at room temperature and -18 ºC treated with GA3 increase the final germination percentages. These results indicated that O. baccatus seeds have physiological dormancy and they need to be stored in order to break their dormancy. In the present study, one year of storage did not show a significant variation in germination between the two storage conditions assayed. Therefore, further research is needed to know about the maximum storage period for O. baccatus seeds under different storage conditions. Very few O. baccatus seeds (less than 5 %) germinated at the tested lowest concentration of NaCl. However, ungerminated seeds were able to germinate when salinity stress was alleviated. In conclusion, O. baccatus seeds have physiological dormancy, and seed storage (at room temperature and at -18 ºC) for one year is effective for breaking this dormancy. In addition, O. baccatus seeds present ability to remain viable in saline conditions and they will be able to germinate once the salinity level decrease.

scholarly journals Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

2021 ◽  
Author(s):  
Hatem Ahmed Shabana ◽  
Tamer Mahmoud ◽  
Sanjay Gairola ◽  
Asma Al Ketbi ◽  
Mariam Aljasmi ◽  
...  
Keyword(s):  
Seed Germination ◽  
United Arab Emirates ◽  
Sodium Hypochlorite ◽  
Room Temperature ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Continuous Darkness ◽  
Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

ADRENALINE AMPOULES QUALITY ASSESSMENT–THE ISRAELI EXPERIENCE

2020 ◽  
pp. 144-146
Author(s):  
MEITAL ZUR ◽  
DAVID STEPENSKY ◽  
PAVEL GORENBEIN
Keyword(s):  
High Performance ◽  
Room Temperature ◽  
Storage Period ◽  
Clinical Effectiveness ◽  
Storage Conditions ◽  
Chiral Hplc ◽  
Drug Products ◽  
Variable Content ◽  
Israel Defense Forces ◽  
Israeli Experience

Objective: To characterize the differences in stability of L-adrenaline in adrenaline ampoules from different manufacturers that are used by the Israel Defense Forces (IDF). Methods: Adrenaline ampoules from three different vendors (Products A, B and C; 52, 13, and 19 batches, respectively) were purchased by the IDF and were stored under the recommended storage conditions (room temperature) for different time periods. The content of L-adrenaline in these samples was determined using a chiral high-performance liquid chromatography (HPLC) assay with UV detection. Results: The three analyzed drug products showed very dissimilar patterns of L-adrenaline degradation. The content of L-adrenaline in Product C was variable and declined below the 85% threshold much earlier than at the end of the 24-months storage period. Products A and B had less variable content of L-adrenaline and were more stable. Conclusion: L-adrenaline is prone to degradation in solution. Its content in adrenaline ampoules from certain vendors can decline rapidly, below the stipulated threshold, and compromise their clinical effectiveness (e. g., during resuscitation). Stability of adrenaline ampoules from individual vendors should be analyzed at different storage conditions, using a chiral HPLC-based assay, to define the shelf-life period that can differ substantially between the vendors.

scholarly journals Stability of Anthocyanins, Phenolics and Color of Tart Cherry Jams

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 255 ◽  
Author(s):  
Vukoja ◽  
Pichler ◽  
Kopjar
Keyword(s):  
Antioxidant Activity ◽  
Sulfonic Acid ◽  
Room Temperature ◽  
Phenolic Content ◽  
Storage Period ◽  
Storage Conditions ◽  
Anthocyanin Content ◽  
Tart Cherry ◽  
Color Parameters ◽  
Textural Parameters

The aim of this work was to assess the effect of a set storage period on the phenolics, anthocyanins, antioxidant activity, color, and texture of three types of jams made from tart cherry. The prepared samples of tart cherry jams were: (1) regular jam; (2) extra jam; and (3) light jam. The samples were stored at room temperature for eight months. Results of the investigated parameters after storage were compared with the results after sample preparations, and between the different jam samples. The light jam had the highest phenolic content and anthocyanin content (3.34 g/kg and 985.52 mg/kg). Consequently, the light jam had the highest antioxidant activity determined by ABTS (2,2'-azinobis(3-ethylbenzothiazoline sulfonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazil) method. After storage, the highest retention of the phenolics had regular jam and extra jam (85%), while the lowest retention (74%) were found in the light jam. Anthocyanin stability was the highest in the regular jam, then the extra jam (15%), and then the light jam, with retention of 22%, 15%, and 12%, respectively. Color parameters and textural parameters also depended on jam type, as well as on storage conditions. Since the investigated types of jams differed in content of fruit and water, these are the most important factors that cause the change in the retention of phenolics and anthocyanins, as well as a change in color and textural parameters.

scholarly journals Egg quality assessment at different storage conditions, seasons and laying hen strains

2017 ◽  
Vol 41 (3) ◽  
pp. 322-333 ◽  
Author(s):  
Vivian Feddern ◽  
Marina Celant De Prá ◽  
Rúbia Mores ◽  
Rodrigo da Silveira Nicoloso ◽  
Arlei Coldebella ◽  
...  
Keyword(s):  
Specific Gravity ◽  
Room Temperature ◽  
Egg Quality ◽  
Storage Period ◽  
Storage Conditions ◽  
Quality Parameters ◽  
Laying Hen ◽  
Unit Weight ◽  
Internal Quality ◽  
Haugh Unit

ABSTRACT Eggs are one of nearly perfect protein foods, offering nutrients of great biological value. However, during storage, egg albumen and yolk components may alter and deteriorate egg quality. Therefore, the aim of this work was to assess egg quality during 9-week storage. Parameters such as Haugh unit, weight loss, egg width and length, specific gravity, yolk and albumen dimensions and their pH were evaluated weekly. A total of 270 eggs (n=5) collected from two different hen strains were evaluated under room (20 to 35 °C in summer; 11.2 to 29.7 °C in autumn) and refrigerated (0 to 5 °C in summer; -3.1 to 6.5 °C in autumn) temperatures. For storage time, an unfolding analysis was accomplished by regression analysis using orthogonal polynomials. As a second approach, principal component analysis (PCA) was performed in order to assess correlations among quality parameters on storage conditions and laying hen strains. By the end of the 9-week storage period, eggs kept under refrigeration presented similar quality parameters to eggs stored at room temperature for only 3 weeks. In contrast, eggs kept at room temperature presented faster degradation from week 1 to 5. No differences on egg quality parameters were noticed between white and brown shells eggs. PCA suggests that better egg quality (first week) was associated mainly with higher egg weight and its specific gravity, Haugh unit and albumen height. Eggs stored at room temperature should be consumed in 2 weeks or refrigerated until 8 weeks, preserving internal quality from farm to retail.

scholarly journals Torrid Panicgrass Dormancy Relief Through Exogenous Chemical Treatments and Seed Storage Optimization of Temperature and Relative Humidity Parameters

2019 ◽  
Vol 29 (2) ◽  
pp. 199-204
Author(s):  
Scott B. Lukas ◽  
Joseph DeFrank ◽  
Orville C. Baldos ◽  
Ruijun Qin
Keyword(s):  
Relative Humidity ◽  
Seedling Survival ◽  
Seed Storage ◽  
Storage Conditions ◽  
Oxygen Intermediates ◽  
Seasonally Dry ◽  
Chemical Treatments ◽  
Long Term Storage ◽  
Physiological Dormancy

Seed dormancy is an evolutionary adaptation for increasing seedling survival by delaying germination and is found in many families of seed plants. Although dormancy is ecologically important, it becomes problematic during agronomic production and restoration. Torrid panicgrass (Panicum torridum) is a native Hawaiian annual grass that has been identified as a re-vegetation candidate for seasonally dry areas. Torrid panicgrass seed appears to possess a nondeep to intermediate physiological dormancy. This research aimed to characterize dormancy relief parameters by 1) evaluating exogenous hormonal, reactive oxygen intermediates, and simulated combustion product treatments; and 2) determining optimized storage conditions of relative humidity (RH) and temperature over a 10-month duration. Results indicate that all exogenous chemical treatments tested were not effective at relieving the dormancy present in torrid panicgrass. Optimal storage conditions to relieve dormancy were found with seeds equilibrated to 12% RH, stored at 30 °C for a period of 8 months resulting in 55% germination. Maintenance of viability for long-term storage up to 10 months was best achieved with seeds stored at 12% RH at 10, 20, or 30 °C.

scholarly journals Effects of Storage Conditions on Postharvest Qualities in Garlic Bulbs (Allium sativum L.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 805C-805
Author(s):  
Sun-Tay Choi ◽  
Ro-Na Bae* ◽  
Dae-Sung Chung ◽  
Seung-Koo Lee
Keyword(s):  
Weight Loss ◽  
Low Temperature ◽  
Pyruvic Acid ◽  
Room Temperature ◽  
Allium Sativum ◽  
Storage Period ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Atmosphere Storage ◽  
And Storage

To investigate quality changes of garlic associated with cultivars and storage conditions, northern type `Seosan' and sub-tropical type `Daeseo' garlics were stored at controlled atmosphere (O2 3%, CO2 5%, -1 ± 1°C) condition, low temperature (-1 ± 1°C), and room temperature (20 ± 5°C). The rate of sprouting, weight loss, enzymatic pyruvic acid content, and degree of greening in crushed garlic were determined during storage. The rate of sprouting was higher in `Daeseo' than in `Seosan' garlic in all storage conditions. Sprouting was effectively suppressed in low temperature and controlled atmosphere storage. Weight loss in `Daeseo' garlic was higher than in `Seosan' garlic. Enzymatic pyruvic acid (EP) contents increased for 3 months storage period, and then decreased gradually as the storage period was prolonged at room or low temperatures. However, EP content decreased dramatically during storage under CA condition in both cultivars. When garlic bulbs were crushed, greening appeared in the garlic stored at low temperature for more than one month. However, greening did not occur in the crushed garlic bulbs stored in CA condition.

scholarly journals Reserve mobilization during imbibition of stored Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds

2011 ◽  
Vol 33 (3) ◽  
pp. 549-560
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Fabrício Francisco Santos da Silva ◽  
...  
Keyword(s):  
Soluble Sugars ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Semi Arid Region

Gliricidia sepium is a drought-tolerant species, easily multiplied by seeds, and has been exploited by farmers as a source of forage in the semi-arid region of northeast Brazil. The objective of the present study was to evaluate the effect of seed storage on the mobilization of reserves during imbibition of "Gliricidia" seeds. Freshly-harvested seeds were packed in kraft paper bags and stored for three and six months in the laboratory under ambient conditions (25 º C ± 3 T and 75% ± 3 RH). Cotyledons were isolated from imbibed seeds and macerated for the extraction and quantification of total soluble sugars, reducing sugars, sucrose and starch, as well as of proteins, amino acids and for amylase activity. Storage under these conditions resulted in an increase in seed water content although germination remained at relatively high levels (86%). Seed macromolecule levels showed significant variation with the storage period and imbibition and these variations were associated with a loss in seed viability due to inadequate storage conditions.

scholarly journals AMMONIA AND NITRATE FLUCTUATIONS IN STORED WATER SAMPLES

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 484e-484
Author(s):  
D.C. Fare ◽  
C.H. Gilliam ◽  
G.J. Keever ◽  
J.T. Touchton
Keyword(s):  
Sulfuric Acid ◽  
Water Samples ◽  
Room Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Nitrate Concentrations

Water samples containing 0, 2.5, 10.0, or 20.0 ppm nitrate and ammonia were evaluated under 3 temperatures (0, 6, 20C) plus or minus sulfuric acid (36N) for changes in concentration. Ammonia and nitrate levels were measured 0, 1, 2, 4, 8, 16, 24, and 32 weeks after storing. Response to storage conditions was the same regardless of acid or concentration of ammonia or nitrate. Nitrate concentrations in the storage locations were similar for the first 2 weeks. Afterwards, treatments stored at room temperature fluctuated from initial standards. With ammonia, frozen samples had the greatest deviation from initial standards during the first 4 weeks. By week 24, ammonia samples stored at room temperature had exceeded acceptable deviations from the standards. Nitrate and ammonia samples held in refrigeration had the least fluctuation during the 32 week storage period.

scholarly journals Changes in the Physicochemical Quality Characteristics of Cabbage Kimchi with respect to Storage Conditions

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Su-Yeon You ◽  
Ji-Su Yang ◽  
Sung Hyun Kim ◽  
In Min Hwang
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Room Temperature ◽  
Gradual Increase ◽  
Storage Period ◽  
Storage Conditions ◽  
Quality Characteristics ◽  
Analytical Technique ◽  
Ph Values ◽  
Organic Acid Content

In this study, the changes in pH, organic acid content, acidity, and salinity of kimchi prepared at 0 days, stored 1–8 weeks at 4 and 10°C, and stored at room temperature for two days were analyzed. Organic acids content was analyzed by using analytical technique of high-performance liquid chromatography (HPLC). This method was also validated using quality assurance parameters of linearity, limits of detection and quantification (LOD and LOQ), precision, and spike recovery experiments. In the analysis of organic acids content (mg/kg), it was found that the kimchi stored for 1–8 weeks at 4 and 10°C showed gradual increase in the organic acids content during storage period. The order of organic acids was lactic acid > acetic acid > citric acid > malic acid > succinic acid > oxalic acid > fumaric acid. The pH values of kimchi stored at 4°C, 10°C, and 25°C were 4.1, 3.6–3.7, and 4.1, respectively.

scholarly journals Investigation of quality indicators and studying the stability of “Propolis-AK” gel for the treatment of acne disease

2019 ◽  
pp. 64-75
Author(s):  
А. I. Tykhonov ◽  
T. G. Yarnykh ◽  
S. G. Bobro ◽  
O. S. Shpychak
Keyword(s):  
Quality Control ◽  
Shelf Life ◽  
Quality Indicators ◽  
Room Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Social Adaptation ◽  
Control Methods ◽  
Aluminum Tubes ◽  
The Stability

In modern conditions, the incidence of acne, which is a polymorphic multifactorial disease of the sebaceous glands of the skin, has a tendency to significant growth. Localization of lesions on the face in almost all patients indicates the fact that acne has an effect on their psycho-emotional sphere and social adaptation, which makes this problem urgent and indicates the feasibility of creating new effective domestic medicines for treating this pathology. The aim of the work was to conduct research on the investigation of quality indicators and studying the stability of «Propolis-AK» gel of anti-inflammatory and antimicrobial action for the treatment of acne disease. The objects of research were model test-samples of «Propolis-AK» gel, for which were developed methodic for analyzing the qualitative composition and quantitative content of the active substances – propolis phenolic hydrophobic drug (PPHD) and azelaic acid (AA) in this dosage form, comprehensively allowing to evaluate the quality and criteria for the stability of the gel during the entire storage period for the following indicators: description, identification, homogeneity, tightness of the container, pH, package contents, microbiological purity, quantification. In addition, the requirements for packaging, labeling, transportation, storage conditions and shelf life were included in the draft of quality control methods. According to the results of the study of organoleptic and physical-chemical parameters of the developed «Propolis-AK» gel during storage at two temperature conditions (8‒15 °C and 15‒25 °C), it was found that the test samples of the gel under study remained fairly stable according to the studied indicators for 2 years and 3 months, which allows us to recommend a shelf life of 2 years at room temperature in aluminum tubes for the studied gel. According to the results of research, a specification for «Propolis-AK» gel for external use was developed as a component of the draft of quality control methods for the studied medicine. Studies have been conducted to establish the main indicators and methods of quality control of the developed «Propolis-AK» gel for the treatment of acne disease. According to the results of the tests, a “Specification” was developed, which was included in the draft of quality control methods and experimentally proved the stability of «Propolis-AK» gel prepared in pharmaceutical and industrial conditions for a prescribed shelf life of 24 months when stored in aluminum tubes with an internal lacquer coating in a cool place (8‒15 °C) and at room temperature (15‒25 °C).

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