scholarly journals Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

2021 ◽  
Author(s):  
Hatem Ahmed Shabana ◽  
Tamer Mahmoud ◽  
Sanjay Gairola ◽  
Asma Al Ketbi ◽  
Mariam Aljasmi ◽  
...  
Keyword(s):  
Seed Germination ◽  
United Arab Emirates ◽  
Sodium Hypochlorite ◽  
Room Temperature ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Continuous Darkness ◽  
Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

1997 ◽  
Vol 33 (2) ◽  
pp. 149-155 ◽  
Author(s):  
A. E. OSMAN ◽  
F. GHASSALI
Keyword(s):  
Seed Germination ◽  
Cold Storage ◽  
North Africa ◽  
Room Temperature ◽  
Storage Temperature ◽  
Seed Storage ◽  
Storage Conditions ◽  
Shrub Species ◽  
Atriplex Halimus ◽  
Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

scholarly journals Seed storage behaviour of Buxus wallichiana Baillon: An important woodcraft species of Indian Himalayan region

2019 ◽  
Vol 11 (2) ◽  
pp. 311-314
Author(s):  
Manisha Thapliyal ◽  
Pravin Rawat
Keyword(s):  
Room Temperature ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Himalayan Region ◽  
Germination Test ◽  
Storage Duration ◽  
Indian Himalayan Region ◽  
Ambient Room Temperature ◽  
Forest Division

Seeds of Buxus wallichiana Baillon are dormant and not much information is available on their shelf life under storage conditions. Therefore, this study was conducted to investigate the seed storage behaviour of B. wallichiana, an important woodcraft species of the Indian Himalayan Region. Healthy matured fruits of B. wallichiana were collected from Matkangra block, Chakrata Forest Division of Uttarakhand. The seeds were extracted, cleaned, processed manually and their initial viability was tested through germination test. After initial viability determination, seeds were processed and placed in air-tight plastic boxes for storage at four temperatures viz., ambient room temperature (Control), 5°C, 15°C and -20°C. Thereafter, at every two months interval, germination test was conducted to assess the viability of the stored seeds. The results revealed that the initial viability of freshly collected seeds was 26.66 per cent and it took 7.83 days to complete germination. After two months of storage the germination percentage increased in the seeds stored at all the temperatures going up to maximum (73.33%) in the ones stored at -20°C followed by 15°C (66.67%) as compared to 50% in the seeds stored at room temperature. Similarly, the mean germination time also increased proportionally, longest being 44 days for seeds stored at -20°C. Seeds stored at ambient room temperature lost viability completely at 6 months storage duration while almost half the viability was lost in seeds stored at 15°C (36.67%) and -20°C (26.67%). From present study, it can be concluded that the longevity of the B. wallichiana seeds is around 12 months when the seeds are stored at lower temperatures, maintaining the viability about 23% at 5°C and 15°C and 16.67 % at  -20°C.

scholarly journals Seed dormancy of Ochradenus baccatus (Resedaceae), a shrubby species from Arabian desert regions

2016 ◽  
Vol 64 (3) ◽  
Author(s):  
Arvind Bhatt ◽  
Félix Pérez-García
Keyword(s):  
Room Temperature ◽  
Water Solution ◽  
Storage Period ◽  
Seed Storage ◽  
Storage Conditions ◽  
Food Sources ◽  
Continuous Darkness ◽  
Physiological Dormancy ◽  
Desert Regions ◽  
One Year

Ochradenus baccatus is a perennial glycophyte growing in Middle East and it is one of the most important food sources for many animal species in desert regions. The aim of our study was to investigate the effects of seed storage, light, temperature and gibberellic acid (GA3) on germination of O. baccatus seeds. We also investigated the germination characteristics of O. baccatus seeds under different saline concentration and their capability to recover germination once they were transferred to distilled water. Seeds were stored at room temperature (20 ± 2 ºC) and at -18 ºC. Germination tests were conducted at alternating temperatures of 15/25, 20/30 and 25/35 ºC in either continuous darkness or photoperiod of 12-h dark/12-h light. To study the effect of GA3 on germination of O. baccatus seeds, freshly-collected seeds and stored seeds were soaked for 24 h in a GA3 water solution (1 g/L) before sowing. To assess the salinity tolerance during germination, seeds were germinated under different salinity levels (100, 200 and 400 mM NaCl). Stored seeds at room temperature and -18 ºC germinate equally well at different temperature regimes and light conditions. However, freshly matured seeds were not able to germinate even when they were treated with GA3. On the contrary, stored seeds at room temperature and -18 ºC treated with GA3 increase the final germination percentages. These results indicated that O. baccatus seeds have physiological dormancy and they need to be stored in order to break their dormancy. In the present study, one year of storage did not show a significant variation in germination between the two storage conditions assayed. Therefore, further research is needed to know about the maximum storage period for O. baccatus seeds under different storage conditions. Very few O. baccatus seeds (less than 5 %) germinated at the tested lowest concentration of NaCl. However, ungerminated seeds were able to germinate when salinity stress was alleviated. In conclusion, O. baccatus seeds have physiological dormancy, and seed storage (at room temperature and at -18 ºC) for one year is effective for breaking this dormancy. In addition, O. baccatus seeds present ability to remain viable in saline conditions and they will be able to germinate once the salinity level decrease.

scholarly journals Effect of Environmental Factors on the Germination and Emergence of Drunken Horse Grass (Achnatherum inebrians)

Weed Science ◽  
2020 ◽  
pp. 1-29
Author(s):  
Yonghuan Yue ◽  
Guili Jin ◽  
Weihua Lu ◽  
Ke Gong ◽  
Wanqiang Han ◽  
...  
Keyword(s):  
Seed Germination ◽  
Environmental Factors ◽  
Abiotic Factors ◽  
Seedling Emergence ◽  
Management Strategies ◽  
Germination Percentage ◽  
Western China ◽  
Burial Depth ◽  
Achnatherum Inebrians ◽  
Water Ph

Abstract Drunken horse grass [Achnatherum inebrians (Hance) Keng] is a perennial poisonous weed in western China. A comprehensive understanding of the ecological response of A. inebrians germination to environmental factors would facilitate the formulation of better management strategies for this weed. Experiments were conducted under laboratory conditions to assess the effects of various abiotic factors, including temperature, light, water, pH and burial depth, on the seed germination and seedling emergence of A. inebrians. The seeds germinated at constant temperatures of 15, 20, 25, 30, 35°C and in alternating-temperature regimes of 15/5, 20/10, 25/15, 30/20, 35/25, 40/30°C, and the seed germination percentages under constant and alternating temperatures ranged from 51% to 94% and 15% to 93%, respectively. Maximum germination occurred at a constant temperature of 25°C, and germination was prevented at 45/35°C. Light did not appear to affect seed germination. The germination percentage of seeds was more than 75% in the pH range of 5 to 10, with the highest germination percentage at pH 6. The seeds germinated at osmotic potentials of 0 MPa to -1.0 MPa, but decreasing osmotic potential inhibited germination, with no germination at -1.2MPa. After 21 d of low osmotic stress, the seeds that did not germinate after rehydration had not lost their vitality. The seedling emergence percentage was highest (90%) when seeds were buried at 1 cm but declined with increasing burial depth and no emergence at 9 cm. Deep tillage may be effective in limiting the seed germination and emergence of this species. The results of this study provide useful information on the conditions necessary for A. inebrians germination and provide a theoretical basis for science-based prediction, prevention and control of this species.

Germination Ecology of Milkweedvine (Morrenia odorata)

Weed Science ◽  
1984 ◽  
Vol 32 (6) ◽  
pp. 781-785 ◽  
Author(s):  
Megh Singh ◽  
Nagi Reddy Achhireddy
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Root Growth ◽  
Seedling Growth ◽  
Shoot Growth ◽  
Seedling Emergence ◽  
Germination Percentage ◽  
Planting Depth ◽  
Germination Ecology ◽  
Optimum Ph

The germination of milkweedvine (Morrenia odorataLindl. ♯3MONOD) seed at 20 or 25 C was unaffected by a 12-h photoperiod. The 12-h photoperiod, however, decreased germination by 50% at 15 C. No germination occurred at 35 C regardless of photoperiod. By alternating 35 C for 12 h with 20 C for 12 h, the germination percentage was 57%. Seedling growth was maximum at alternating temperatures of 30/20 C. Optimum pH for germination and seedling growth was 7 and germination did not occur at pH levels below 6. Seed germination declined steadily at osmotic stress below −0.12 MPa; no germination occurred at −0.5 MPa. Seedling growth was not influenced by osmotic stress down to −0.18 MPa. Germination percentages of seeds kept under aerated water and nonaerated water were similar, but the seedling growth was greater in aerated water. Seedling emergence was maximum from depths of 0.5 to 2.5 cm, but no seedling emerged from 0 or 10 cm. Planting depth was negatively correlated (r = −0.7) with shoot growth but positively correlated (r = +0.98) to root growth.

scholarly journals Factors Affecting Seed Dormancy and Germination of Greater Bur-Parsley (Turgenia latifolia)

2018 ◽  
Vol 36 ◽  
Author(s):  
M. REZVANI ◽  
S.A. SADATIAN ◽  
H. NIKKHAHKOUCHAKSARAEI
Keyword(s):  
Seed Germination ◽  
Environmental Factors ◽  
Seed Dormancy ◽  
Seedling Emergence ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Planting Depth ◽  
Factors Affecting ◽  
Drought And Salt Stresses

ABSTRACT: Our knowledge about seed dormancy breaking and environmental factors affecting seed germination of greater bur-parsley (Turgenia latifolia) is restricted. This study has addressed some seed dormancy breaking techniques, including different concentrations of gibberellic acid (GA3) and potassium nitrate (KNO3), leaching duration, physical scarification as well as some environmental factors effective on seed germination such as salt and drought stresses, pH and seed planting depth. Seed germination was promoted with lower concentrations of KNO3 (0.01 to 0.02 g L-1), while higher concentrations reduced germination percentage. Seed dormancy was declined by low concentrations of GA3 up to 100 ppm. Seeds of greater bur-parsley germinated in a range of pH from 3 to 7. With enhancement of drought and salt stresses, seed germination decreased. Also, there was no seed germination in a high level of stresses. Seedling emergence reduced as planting depth increased. Use of GA3, KNO3, leaching and physical scarification had a positive effect on seed dormancy breaking of greater bur-parsley. The information from the study increases our knowledge about seed dormancy breaking techniques, response of germination to drought and salt stresses and also determination of distribution regions of greater bur-parsley in the future.

scholarly journals The Effect of Priming Treatments on Germination and Seedling Performance of Purslane (Portulaca oleracea) Seed Lots

2017 ◽  
Vol 74 (2) ◽  
pp. 164
Author(s):  
Eren OZDEN ◽  
Ibrahim DEMIR
Keyword(s):  
Seed Germination ◽  
Relative Humidity ◽  
Seedling Emergence ◽  
Germination Percentage ◽  
Portulaca Oleracea ◽  
Distilled Water ◽  
Climate Chamber ◽  
Untreated Seed ◽  
Percent Relative Humidity ◽  
Purslane Seed

This study was conducted to test the effect of a priming combination on the seed germination percentage and seedling emergence performance of purslane under climate chamber and field conditions. Four purslane seed lots were treated according five different methods, which were T1: Seeds kept at a hundred percent relative humidity for four hours at 20 °C; T2: Seeds kept at a hundred percent relative humidity for four hours at 20 °C, and then soaked in distilled water for 8 hours at 5 °C; T3: Seeds kept at a hundred percent relative humidity for four hours at 20 °C, and then soaked in distilled water for 8 hours at 20 °C; T4: Seeds soaked in distilled water for 8 hours at 5 °C; T5: Seeds soaked in distilled water for 8 hours at 20 °C; and C: Control (untreated). Seed germination was calculated for 14 days at 20 °C, seedling emergence percentages were calculated in the climatically-controlled chamber for 21 days at 22 °C, and in the field for 35 days at 15-25 °C. The highest seed germination (94%) and seedling emergence in the climatically-controlled chamber (87%) and field (82%) were obtained from seeds that had been kept at a hundred percent relative humidity for four hours at 20 °C, then soaked in distilled water for eight hours at 5 °C. Results indicated that farm-priming, can be an efficient priming method in purslane seeds.

Factors Affecting Buffalobur (Solanum rostratum) Seed Germination and Seedling Emergence

Weed Science ◽  
2009 ◽  
Vol 57 (5) ◽  
pp. 521-525 ◽  
Author(s):  
Shouhui Wei ◽  
Chaoxian Zhang ◽  
Xiangju Li ◽  
Hailan Cui ◽  
Hongjuan Huang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Light Exposure ◽  
Soil Depth ◽  
Seedling Emergence ◽  
Soil Surface ◽  
Distribution Area ◽  
Burial Depth ◽  
Weed Species ◽  
Continuous Darkness ◽  
Invasive Weed

Buffalobur is a noxious and invasive weed species native to North America. The influence of environmental factors on seed germination and seedling emergence of buffalobur were evaluated in laboratory and greenhouse experiments. The germination of buffalobur seeds occurred at temperatures ranging from 12.5 to 45 C, with optimum germination attained between 25 and 35 C. Buffalobur seeds germinated equally well under both a 14-h photoperiod and continuous darkness; however, prolonged light exposure (≥ 16 h) significantly inhibited the seed germination. Buffalobur seed is rather tolerant to low water potential and high salt stress, as germination was 28 and 52% at osmotic potentials of −1.1 MPa and salinity level of 160 mM, respectively. Medium pH has no significant effect on seed germination; germination was greater than 95% over a broad pH range from 3 to 10. Seedling emergence was higher (85%) for seeds buried at a soil depth of 2 cm than for those placed on the soil surface (32%), but no seedlings emerged when burial depth reached 8 cm. Knowledge of germination biology of buffalobur obtained in this study will be useful in predicting the potential distribution area and developing effective management strategies for this species.

Dispersal and germination syndromes of tree seeds in a monsoonal forest in northern India

2005 ◽  
Vol 15 (1) ◽  
pp. 29-42 ◽  
Author(s):  
R.C. Thapliyal ◽  
S.S. Phartyal
Keyword(s):  
Seed Germination ◽  
Seed Storage ◽  
Germination Percentage ◽  
Cold Season ◽  
Poor Quality ◽  
Northern India ◽  
High Germination ◽  
Monsoon Forest ◽  
Mean Germination Time ◽  
Pre Treatment

This paper describes the dispersal–germination characteristics of seeds of 77 native tree species in a seasonal monsoon forest in Uttaranchal state, northern India. Results indicate that 50% of the species dispersed in the hot, dry summer months, 18% during the rainy season, 23% during the cold season and the remainder in late spring. Germination tests on fresh and laboratory-stored seeds revealed a relationship between morphological features of the fruit and both germination percentage and mean germination time (MGT). Highest mean germination (50%) was for dry-dehiscent fruits with winged wind-dispersed seeds, followed by dry-dehiscent fruits with non-winged seeds (38%) and seeds of dry-indehiscent fruits (37%). Lowest germination (29%) was for seeds from fleshy or pulpy fruits. MGT followed the reverse course. Germination data for seeds stored dry in the laboratory during one seeding cycle indicated six patterns of seed germination: (1) average germination percentage of fresh seeds lower than that of stored seeds, indicating an after-ripening requirement; (2) initial high germination percentage followed by low values, indicating a steep to moderate decline in viability following harvest; (3) no germination after 1 or 2 months of seed storage, due to complete loss of viability, indicating short seed longevity; (4) fresh seed germination in some species equalled the average germination value of stored seeds, indicating constant germination for one whole seeding cycle; (5) germination of both fresh and stored seeds remained consistently low throughout the season, indicating a requirement for some kind of pre-treatment or having poor quality of seeds; (6) initial high germination followed by decline and again increase, showing a seasonal rhythm of germination.

scholarly journals Seed and Embryo Germination in Ardisia crenata

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Takahiro Tezuka ◽  
Hisa Yokoyama ◽  
Hideyuki Tanaka ◽  
Shuji Shiozaki ◽  
Masayuki Oda
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Critical Factor ◽  
Seed Storage ◽  
Storage Conditions ◽  
Embryo Germination ◽  
Long Time ◽  
Dry Conditions ◽  
Temperature Water ◽  
Good Germination

Ardisia crenata is an evergreen shrub with attractive bright red berries. Although this species is usually propagated by seed, the seeds take a long time to germinate with conventional sowing methods. We investigated the germination capacity of seeds and embryos collected in different months and the effects of seed storage conditions, germination temperature, water permeability of the seed coat, and the endosperm on seed germination. Seeds and embryos collected in late September or later showed good germination rates. Seeds germinated more rapidly after longer periods of storage at low temperature (approximately 5°C), and those stored in dry conditions showed lower emergence frequency than those stored in wet conditions. Seeds germinated at 15–30°C, but not at 5–10°C. Removal of the seed coat enhanced water uptake and seed germination. Seeds with various proportions of the removed seed coat were sown on a medium supplemented with sucrose. The germination frequency increased as the size of the remaining endosperm decreased. However, the opposite results were obtained when seeds were sown on a medium without sucrose. We concluded that the optimal temperature of 25°C is the most critical factor for seed germination in A. crenata.

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