Effect of a plant-originated natural compound, CoD™ extract, on c-myc, Ha-ras and p53 gene expression in short-term animal experiments

2008 ◽  
Vol 37 (1) ◽  
pp. 87-92
Author(s):  
E. Nádasi ◽  
I. Prantner ◽  
T. Dávid ◽  
I. Ember
Author(s):  
Toshihiro Sakurai ◽  
Zhen Chen ◽  
Arisa Yamahata ◽  
Takahiro Hayasaka ◽  
Hiroshi Satoh ◽  
...  

2021 ◽  
Vol 21 (4) ◽  
pp. 1-28
Author(s):  
Song Deng ◽  
Fulin Chen ◽  
Xia Dong ◽  
Guangwei Gao ◽  
Xindong Wu

Load forecasting in short term is very important to economic dispatch and safety assessment of power system. Although existing load forecasting in short-term algorithms have reached required forecast accuracy, most of the forecasting models are black boxes and cannot be constructed to display mathematical models. At the same time, because of the abnormal load caused by the failure of the load data collection device, time synchronization, and malicious tampering, the accuracy of the existing load forecasting models is greatly reduced. To address these problems, this article proposes a Short-Term Load Forecasting algorithm by using Improved Gene Expression Programming and Abnormal Load Recognition (STLF-IGEP_ALR). First, the Recognition algorithm of Abnormal Load based on Probability Distribution and Cross Validation is proposed. By analyzing the probability distribution of rows and columns in load data, and using the probability distribution of rows and columns for cross-validation, misjudgment of normal load in abnormal load data can be better solved. Second, by designing strategies for adaptive generation of population parameters, individual evolution of populations and dynamic adjustment of genetic operation probability, an Improved Gene Expression Programming based on Evolutionary Parameter Optimization is proposed. Finally, the experimental results on two real load datasets and one open load dataset show that compared with the existing abnormal data detection algorithms, the algorithm proposed in this article have higher advantages in missing detection rate, false detection rate and precision rate, and STLF-IGEP_ALR is superior to other short-term load forecasting algorithms in terms of the convergence speed, MAE, MAPE, RSME, and R 2 .


1999 ◽  
Vol 276 (4) ◽  
pp. C946-C954 ◽  
Author(s):  
Li Li ◽  
Ji Li ◽  
Jaladanki N. Rao ◽  
Minglin Li ◽  
Barbara L. Bass ◽  
...  

The nuclear phosphoprotein p53 acts as a transcription factor and is involved in growth inhibition and apoptosis. The present study was designed to examine the effect of decreasing cellular polyamines on p53 gene expression and apoptosis in small intestinal epithelial (IEC-6) cells. Cells were grown in DMEM containing 5% dialyzed fetal bovine serum in the presence or absence of α-difluoromethylornithine (DFMO), a specific inhibitor of polyamine biosynthesis, for 4, 6, and 12 days. The cellular polyamines putrescine, spermidine, and spermine in DFMO-treated cells decreased dramatically at 4 days and remained depleted thereafter. Polyamine depletion by DFMO was accompanied by a significant increase in expression of the p53 gene. The p53 mRNA levels increased 4 days after exposure to DFMO, and the maximum increases occurred at 6 and 12 days after exposure. Increased levels of p53 mRNA in DFMO-treated cells were paralleled by increases in p53 protein. Polyamines given together with DFMO completely prevented increased expression of the p53 gene. Increased expression of the p53 gene in DFMO-treated cells was associated with a significant increase in G1 phase growth arrest. In contrast, no features of programmmed cell death were identified after polyamine depletion: no internucleosomal DNA fragmentation was observed, and no morphological features of apoptosis were evident in cells exposed to DFMO for 4, 6, and 12 days. These results indicate that 1) decreasing cellular polyamines increases expression of the p53 gene and 2) activation of p53 gene expression after polyamine depletion does not induce apoptosis in intestinal crypt cells. These findings suggest that increased expression of the p53 gene may play an important role in growth inhibition caused by polyamine depletion.


2008 ◽  
Vol 32 (2) ◽  
pp. 219-228 ◽  
Author(s):  
Adeel Safdar ◽  
Nicholas J. Yardley ◽  
Rodney Snow ◽  
Simon Melov ◽  
Mark A. Tarnopolsky

Creatine monohydrate (CrM) supplementation has been shown to increase fat-free mass and muscle power output possibly via cell swelling. Little is known about the cellular response to CrM. We investigated the effect of short-term CrM supplementation on global and targeted mRNA expression and protein content in human skeletal muscle. In a randomized, placebo-controlled, crossover, double-blind design, 12 young, healthy, nonobese men were supplemented with either a placebo (PL) or CrM (loading phase, 20 g/day × 3 days; maintenance phase, 5 g/day × 7 days) for 10 days. Following a 28-day washout period, subjects were put on the alternate supplementation for 10 days. Muscle biopsies of the vastus lateralis were obtained and were assessed for mRNA expression (cDNA microarrays + real-time PCR) and protein content (Kinetworks KPKS 1.0 Protein Kinase screen). CrM supplementation significantly increased fat-free mass, total body water, and body weight of the participants ( P < 0.05). Also, CrM supplementation significantly upregulated (1.3- to 5.0-fold) the mRNA content of genes and protein content of kinases involved in osmosensing and signal transduction, cytoskeleton remodeling, protein and glycogen synthesis regulation, satellite cell proliferation and differentiation, DNA replication and repair, RNA transcription control, and cell survival. We are the first to report this large-scale gene expression in the skeletal muscle with short-term CrM supplementation, a response that suggests changes in cellular osmolarity.


Author(s):  
J. Bourhis ◽  
J. Bosq ◽  
G.D. Wilson ◽  
B. Bressac ◽  
P. Wibault ◽  
...  

2018 ◽  
Vol 189 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Andreas Lamkowski ◽  
Matthias Kreitlow ◽  
Jörg Radunz ◽  
Martin Willenbockel ◽  
Frank Sabath ◽  
...  

2001 ◽  
Vol 34 (5) ◽  
pp. 415-420 ◽  
Author(s):  
George J Soleas ◽  
David M Goldberg ◽  
Linda Grass ◽  
Michael Levesque ◽  
Eleftherios P Diamandis

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