scholarly journals Concentrations of retinol and carotenoids in Jersey milk during different seasons and possible application of the colour parameter as an indicator of milk carotenoid content

2020 ◽  
Vol 70 (4) ◽  
pp. 266-274
Author(s):  
Nikolina Kelava Ugarković ◽  
Zvonimir Prpić ◽  
Miljenko Konjačić ◽  
Ivan Vnučec ◽  
Tomislav Rusan
Keyword(s):  
Chemical Composition ◽  
Milk Fat ◽  
High Performance ◽  
Cow Milk ◽  
Carotenoid Content ◽  
Yellow Colour ◽  
Grazing Season ◽  
Colour Parameter ◽  
Different Seasons ◽  
Β Carotene

The aim of this study was to determine concentrations of retinol, β-carotene, lutein and zeaxanthin in Jersey milk during different seasons and their correlation with milk colour parameters. During a sixmonths period the Jersey cows (n=77) were kept free-stall and fed with total mixed ratio (TMR; farm season), for the next three months the cows were kept for four hours on pasture and the rest of the day free-stall fed with TMR (transition season), and for the remaining three months they were kept predominantly on pasture (grazing season). A bulk milk samples (200 mL) were collected during the last week of each month in the period between May 2018 and April 2019. Retinol and carotenoids concentrations in cow milk were quantified using high performance liquid chromatography, while milk chemical composition was analysed by a MilkoScan FT 120 infra-red analyser. The season significantly (P<0.05) affected chemical composition of milk, and the highest (P<0.05) content of milk fat, protein and non-fat dry matter was found during the farm season. Retinol, β-carotene, lutein and zeaxanthin concentrations (μg/100 mL) were as follows: 2.56±0.29, 4.15±0.27, 4.43±0.40 and 0.97±0.04. Lutein and retinol concentrations in Jersey milk were affected (P<0.05) by the season. The highest lutein concentration was found during the grazing season, while the farm season yielded the highest retinol concentration. Moderate and positive correlations (P<0.05) were found between β-carotene and lutein concentrations and colour parameter b*, resulting in a more intense yellow colour i.e. high values of colour parameter b*. The obtained results suggest that milk colour could be used to estimate carotenoid concentrations in milk.

scholarly journals Soil Nutrient Management Practices Influence the Carotenoid Content and Profile of Orange Fleshed Sweet Potato Variety (UMUSPO 3)

2020 ◽  
Vol 8 ◽  
pp. 38-46
Author(s):  
Philippa Chinyere Ojimelukwe ◽  
Udo U. Okpanku
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Management Practices ◽  
Nutrient Management ◽  
Poultry Manure ◽  
Soil Nutrient ◽  
Soil Management ◽  
Soil Treatment ◽  
Carotenoid Content ◽  
Β Carotene

Food bio fortification holds a lot of promise for the alleviation of hidden hunger. The appropriate soil nutrient management methods that will maximize the pro vitamin A content of bio fortified Orange fleshed sweet potatoes bio fortified was evaluated in the present research. The carotenoid content and profile of OFSP variety grown on soils given five different nutrient managements were compared. The soil management treatments given were: Poultry manure at 10 x 103 kg /ha (VB1); Poultry manure at 5 x 103 kg/ha;( VB2): Poultry manure at 2.5 x 103 kg/ha + NPK at 200 kg/ha + Agrolyser at 2.7 kg/ha, (VB3) Agrolyser at 2.7 kg/ha + NPK at 200 kg/ha; (VB4) Poultry manure at 5.0 x 103 kg/ha + NPK at 200 kg/ha, (VB5) NPK 15:15:15 at 400 kg/ha. Carotenoids were extracted from the potato samples and analyzed using High performance Liquid Chromatography (HPLC). The best treatment that promoted high pro vitamin A carotenoid content was soil treatment with Poultry manure (5x 103 kg/ha+ NPK (200kg/ha). This soil treatment led to the highest contents of α-carotene (6.14μg/g); 13-cis-β-carotene (12.36μg/g); All-trans-β-carotene (87.89μg/g) and 9-cis-β-carotene (2.99μg/g). The best soil management treatment for the highest yield of β-cryptoxanthin (7.95μg/g) was poultry manure at 2.5kg/ha +NPK at 200kg/ha.

scholarly journals Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation

2020 ◽  
Vol 15 (1) ◽  
pp. 27-34
Author(s):  
Shilpa Pandurangaiah ◽  
Sadashiva A T ◽  
Shivashankar K S ◽  
SudhakarRao D V ◽  
Ravishankar K V
Keyword(s):  
High Performance ◽  
Color Space ◽  
Nondestructive Method ◽  
Carotenoid Content ◽  
Total Carotenoids ◽  
Time Saving ◽  
Carotene Content ◽  
Spectrophotometric Methods ◽  
Β Carotene ◽  
Cherry Tomatoes

Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin AA carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage.

scholarly journals The Study of Variation in Fatty Acids Composition for Animal Fats

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Flavia POP ◽  
Cornel LASLO
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Polyunsaturated Fatty Acids ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Cow Milk ◽  
Animal Fats ◽  
Poultry Fat

In this article the chemical composition of 2 types of animal fats (cow milk fat and poultry fat) following the variation of saturated and unsaturated fatty acids proportion during freezing storage was studied. Determination of chemical composition of animal fats is important in establishing organoleptic and physico-chemical parameters, the variation of them in time, nature and proportion of fatty acids conferring specific characteristics to them. For milk fat was determined the following chemical composition: saturated fatty acids 68.35%, monounsaturated fatty acids 29.25%, polyunsaturated fatty acids 2.4%. After 4 months of storage under freezing there was a change in fatty acids proportion, saturated fatty acid content increased to 70.41%, monounsaturated fatty acids content decreased to 28.23%, and polyunsaturated fatty acids content decreased to 1.35% due to oxidation process when decreased the degree of unsaturation due to unsaturated fatty acids oxidation. In the case of poultry fat there was also an increase of saturated fatty acids (30.71%) and a decrease for monounsaturated (43.47%) and polyunsaturated (24.81%) fatty acids content.

scholarly journals Carotenoid Content in Breastmilk in the 3rd and 6th Month of Lactation and Its Associations with Maternal Dietary Intake and Anthropometric Characteristics

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 193 ◽  
Author(s):  
Monika Zielinska ◽  
Jadwiga Hamulka ◽  
Aleksandra Wesolowska
Keyword(s):  
Dietary Intake ◽  
Regression Models ◽  
High Performance ◽  
Maternal Diet ◽  
Inverse Correlation ◽  
Carotenoid Content ◽  
Dietary Record ◽  
Maternal Bmi ◽  
Maternal Dietary Intake ◽  
Β Carotene

Carotenoids are diet-dependent milk components that are important for the visual and cognitive development of an infant. This study determined β-carotene, lycopene and lutein + zeaxanthin in breastmilk and its associations with dietary intake from healthy Polish mothers in the first six months of lactation. Concentrations of carotenoids in breastmilk were measured by HPLC (high-performance liquid chromatography) (first, third, sixth month of lactation) and dietary intake was assessed based on a three-day dietary record (third and sixth month of lactation). The average age of participants (n = 53) was 31.4 ± 3.8 years. The breastmilk concentrations of carotenoids were not changed over the progress of lactation. Lycopene was a carotenoid with the highest content in breastmilk (first month 112.2 (95% CI 106.1–118.3)—sixth month 110.1 (103.9–116.3) nmol/L) and maternal diet (third month 7897.3 (5465.2–10329.5) and sixth month 7255.8 (5037.5–9474.1) µg/day). There was a positive correlation between carotenoids in breastmilk and dietary intake (lycopene r = 0.374, r = 0.338; lutein + zeaxanthin r = 0.711, r = 0.726, 3rd and 6th month, respectively) and an inverse correlation with maternal BMI in the third month of lactation (β-carotene: r = −0.248, lycopene: r = −0.286, lutein + zeaxanthin: r = −0.355). Adjusted multivariate regression models confirmed an association between lutein + zeaxanthin intake and its concentration in breastmilk (third month: β = 0.730 (0.516–0.943); 6th: β = 0.644 (0.448–0.840)). Due to the positive associations between dietary intake and breastmilk concentrations, breastfeeding mothers should have a diet that is abundant in carotenoids.

scholarly journals Evaluation of Quality and Acceptability of Snack (Kokoro) Produced From Synthetic Provitamin A Maize (Zea mays) Genotypes

2020 ◽  
Vol 4 ◽  
Author(s):  
Omololami Tolulope Akinsola ◽  
Emmanuel Oladeji Alamu ◽  
Bolanle Omolara Otegbayo ◽  
Abebe Menkir ◽  
Busie Maziya-Dixon
Keyword(s):  
Chemical Composition ◽  
Vitamin A ◽  
Age Groups ◽  
Provitamin A ◽  
Carotenoid Content ◽  
Percentage Contribution ◽  
Unit Operations ◽  
Maize Genotypes ◽  
Daily Allowance ◽  
Β Carotene

Kokoro from provitamin A (PVA) maize genotypes, produced through conventional breeding, was studied to improve the indigenous white maize-based snack deficient in provitamin A carotenoid commonly consumed in South-western Nigeria. The chemical composition, carotenoid retention, and acceptability of Kokoro from three PVA maize genotypes and one landrace yellow maize variety (control) were estimated. Chemical composition showed significant differences (p < 0.05) in parameters with high crude fat content (23.21–32.11%). The sensory evaluation result revealed that Kokoro from DT STR SYN2-Y (control) was the most acceptable, while among the PVA Kokoro, PVA SYN HGBC1 was acceptable. The pre-processing for the estimated carotenoids (μg g−1); lutein, zeaxanthin, total β-carotene, and PVA in maize genotypes ranged from 10.38 to 12.87, 6.03 to 10.97, 3.83 to 6.18, and 5.96 to 8.43, while after processing to Kokoro, total β-carotene ranged from 1.47 to 3.10 μg g−1 and total PVA content 2.43–4.00 μg g−1. The carotenoid retention in Kokoro from PVA maize genotypes ranged from zeaxanthin 5.89–8.39%; lutein 2.74–4.45%; total β-carotene 38.24–66.14%, and total PVA 37.98–67.05%. Degradation of carotenoid was observed due to the unit operations in the processing method that led to the exposure of the food matrix to direct sunlight, heat, light, metals, and oxygen resulting in the formation of cis-isomers and loss of provitamin A quantity. The maize genotype PVASYNHGBC0 had the highest PVA value and carotenoid retention after processing. The study observed that PVA retention of Kokoro was genotype-dependent, and genotype PVASYNHGBC0 (Provitamin A maize HGA cycle zero) retained the highest carotenoid content. Also, PVASYNHGBC0 (for all the servings' size; 100 and 150 g) in all age groups had the highest percentage contribution of vitamin A to the recommended daily allowance. However, further improvement in the carotenoid content of maize genotypes is needed to enable the production of nutritious Kokoro with higher vitamin A percentage contribution and retinol equivalent.

Determination of Carotenoids in Yellow Maize, the Effects of Saponification and Food Preparations

2008 ◽  
Vol 78 (3) ◽  
pp. 112-120 ◽  
Author(s):  
Muzhingi ◽  
Yeum ◽  
Russell ◽  
Johnson ◽  
Qin ◽  
...  
Keyword(s):  
High Performance ◽  
Provitamin A ◽  
Carotenoid Content ◽  
Carotenoid Concentration ◽  
Eye Health ◽  
Provitamin A Carotenoids ◽  
C30 Column ◽  
Β Carotene ◽  
Food Consumed

Maize is an important staple food consumed by millions of people in many countries. Yellow maize naturally contains carotenoids which not only provide provitamin A carotenoids but also xanthophylls, which are known to be important for eye health. This study was aimed at 1) evaluating the effect of saponification during extraction of yellow maize carotenoids, 2) determining the major carotenoids in 36 genotypes of yellow maize by high-performance liquid chromatography with a C30 column, and 3) determining the effect of cooking on the carotenoid content of yellow maize. The major carotenoids in yellow maize were identified as all-trans lutein, cis-isomers of lutein, all-trans zeaxanthin, α- and β-cryptoxanthin, all-trans β-carotene, 9-cis β-carotene, and 13-cis β-carotene. Our results indicated that carotenoid extraction without saponification showed a significantly higher yield than that obtained using saponification. Results of the current study indicate that yellow maize is a good source of provitamin A carotenoids and xanthophylls. Cooking by boiling yellow maize at 100° C for 30 minutes increased the carotenoid concentration, while baking at 450° F for 25 minutes decreased the carotenoid concentrations by almost 70% as compared to the uncooked yellow maize flour.

scholarly journals Genetic Variation of Beta-carotene and Lutein Contents in Lettuce

2005 ◽  
Vol 130 (6) ◽  
pp. 870-876 ◽  
Author(s):  
Beiquan Mou
Keyword(s):  
Genetic Variation ◽  
High Performance ◽  
Beta Carotene ◽  
Carotenoid Content ◽  
Color Measurement ◽  
Primitive Form ◽  
Carotenoid Concentration ◽  
Highly Correlated ◽  
Wild Accessions ◽  
Β Carotene

There is increasing medical evidence for the health benefits derived from dietary intake of carotenoid antioxidants, such as β-carotene and lutein. Enhancing the nutritional levels of vegetables would improve the nutrient intake without requiring an increase in consumption. A breeding program to improve the nutritional quality of lettuce (Lactuca sativa L.) must start with an assessment of the existing genetic variation. To assess the genetic variability in carotenoid contents, 52 genotypes including crisphead, leaf, romaine, butterhead, primitive, Latin, and stem lettuces, and wild species were planted in the field in Salinas, Calif., in the Summer and Fall of 2003 with four replications. Duplicate samples from each plot were analyzed for chlorophyll (a and b), β-carotene, and lutein concentrations by high-performance liquid chromatography (HPLC). Wild accessions (L. serriola L., L. saligna L., L. virosa L., and primitive form) had higher β-carotene and lutein concentrations than cultivated lettuces, mainly due to the lower moisture content of wild lettuces. Among major types of cultivated lettuce, carotenoid concentration followed the order of: green leaf or romaine > red leaf > butterhead > crisphead. There was significant genetic variation in carotenoid concentration within each of these lettuce types. Crisphead lettuce accumulated more lutein than β-carotene, while other lettuce types had more β-carotene than lutein. Carotenoid concentration was higher in summer than in the fall, but was not affected by the position of the plant on the raised bed. Beta-carotene and lutein concentrations were highly correlated, suggesting that their levels could be enhanced simultaneously. Beta-carotene and lutein concentrations were both highly correlated with chlorophyll a, chlorophyll b, and total chlorophyll concentrations, suggesting that carotenoid content could be selected indirectly through chlorophyll or color measurement. These results suggest that genetic improvement of carotenoid levels in lettuce is feasible.

scholarly journals Retinal Biosynthesis in Fungi: Characterization of the Carotenoid Oxygenase CarX from Fusarium fujikuroi

2007 ◽  
Vol 6 (4) ◽  
pp. 650-657 ◽  
Author(s):  
Alfonso Prado-Cabrero ◽  
Daniel Scherzinger ◽  
Javier Avalos ◽  
Salim Al-Babili
Keyword(s):  
High Performance ◽  
Feedback Mechanism ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Carotenoid Content ◽  
Fusarium Fujikuroi ◽  
Negative Feedback Mechanism ◽  
Total Carotenoid Content ◽  
Β Carotene

ABSTRACT The car gene cluster of the ascomycete Fusarium fujikuroi encodes two enzymes responsible for torulene biosynthesis (CarRA and CarB), an opsin-like protein (CarO), and a putative carotenoid cleaving enzyme (CarX). It was presumed that CarX catalyzes the formation of the major carotenoid in F. fujikuroi, neurosporaxanthin, a cleavage product of torulene. However, targeted deletion of carX did not impede neurosporaxanthin biosynthesis. On the contrary, ΔcarX mutants showed a significant increase in the total carotenoid content, indicating an involvement of CarX in the regulation of the pathway. In this work, we investigated the enzymatic activity of CarX. The expression of the enzyme in β-carotene-accumulating Escherichia coli cells led to the formation of the opsin chromophore retinal. The identity of the product was proven by high-performance liquid chromatography and gas chromatography-mass spectrometry. Subsequent in vitro assays with heterologously expressed and purified CarX confirmed its β-carotene-cleaving activity and revealed its capability to produce retinal also from other substrates, such as γ-carotene, torulene, and β-apo-8′-carotenal. Our data indicate that the occurrence of at least one β-ionone ring in the substrate is required for the cleavage reaction and that the cleavage site is determined by the distance to the β-ionone ring. CarX represents the first retinal-synthesizing enzyme reported in the fungal kingdom so far. It seems likely that the formed retinal is involved in the regulation of the carotenoid biosynthetic pathway via a negative feedback mechanism.

Uncultivated Brazilian Green Leaves are Richer Sources of Carotenoids than are Commercially Produced Leafy Vegetables

2008 ◽  
Vol 29 (4) ◽  
pp. 320-328 ◽  
Author(s):  
Cintia Nanci Kobori ◽  
Delia B. Rodriguez Amaya
Keyword(s):  
High Performance ◽  
Vitamin A Deficiency ◽  
Hplc Method ◽  
Leafy Vegetables ◽  
Carotenoid Content ◽  
International Programs ◽  
Β Carotene ◽  
Coriander Leaves

Background With the continuing problem of vitamin A deficiency, the recognition of the role of carotenoids in disease prevention, and international programs promoting biodiversity, determination of the carotenoid content of indigenous Brazilian foods is needed. Objective To determine the principal carotenoids in native leaves and compare the levels with those in commercially produced leafy vegetables. Methods The indigenous Brazilian leafy vegetables caruru, mentruz, taioba, serralha, and beldroega were analyzed with the use of a previously developed and validated high-performance liquid chromatography (HPLC) method. Parsley and coriander leaves, which were previously shown to be the richest in carotenoids among commercially produced leaves, were analyzed for comparison. Five sample lots of each vegetable collected at different times during the year were analyzed immediately after harvest. Results Lutein concentrations were 119 ± 21, 111 ± 48, 104 ± 44, 87 ± 7, and 34 ± 14 μg/g, and β-carotene contents were 114 ± 22, 97 ± 40, 66 ± 18, 72 ± 9, and 32 ± 14 μg/g for caruru, mentruz, taioba, serralha, and beldroega, respectively. Except for beldroega, these values were higher than those for commercial leaves. Parsley had 88 ± 18 μg/g of lutein and 65 ± 13 μg/g of β-carotene. Coriander leaves contained 74 ± 6 μg/g of lutein and 55 ± 5 μg/g of β-carotene. The violaxanthin and neoxanthin concentrations were also higher in the native leaves. Comparison with values for previously analyzed commercial leafy vegetables confirmed the higher carotenoid levels of the native leaves. Conclusions The indigenous leaves investigated are richer sources of carotenoids than are commercially produced leafy vegetables.

scholarly journals Variation in β-Carotene and Total Carotenoid Content in Fruits of Capsicum

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 746-749 ◽  
Author(s):  
Marisa M. Wall ◽  
Cynthia A. Waddell ◽  
Paul W. Bosland
Keyword(s):  
High Performance ◽  
Dry Weight ◽  
Carotenoid Content ◽  
Total Carotenoid ◽  
Fresh Weight ◽  
Ripe Fruit ◽  
Total Carotenoids ◽  
New Mexican ◽  
Total Carotenoid Content ◽  
Β Carotene

The β-carotene and total carotenoid content of either fresh or dried tissue of fruits of a total of 57 cultivars of six Capsicum species were analyzed using high performance liquid chromatography (HPLC). β-Carotene levels in ripe fruit varied from 0 to 166 μg·g-1 fresh weight, and carotenoid levels were from 1 to 896 μg·g-1 in ripe fruit in 1996. The range of values for β-carotene was similar in 1997, but that for total carotenoids was wider (4 to 1173 μg·g-1 fresh weight). Fresh fruit of the cultivars Greenleaf Tabasco, Pulla, Guajillo, NuMex Conquistador, Ring-O-Fire, and Thai Dragon contained greater amounts of β-carotene per 100 g fresh weight than the recommended dietary allowance (RDA) for vitamin A for the average adult. For dried Capsicum entries, New Mexican, aji, pasilla, and ancho types had the highest levels of β-carotene. In 1996, β-carotene levels among the dried Capsicum germplasm ranged from 2 to 739 μg·g-1 dry weight, and carotenoid levels from 111 to 6226 μg·g-1. Values were higher in 1997, ranging from 24 to 1198 μg·g-1 dry weight for β-carotene and from 187 to 10,121 μg·g-1 for total carotenoids. A pasilla type (C. annuum L.) had the highest total carotenoid content among the dried entries in both years.

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