scholarly journals Biocompatible Parylene Neurocages for Action Potential Recording and Stimulation

2006 ◽  
Vol 926 ◽  
Author(s):  
Angela Tooker ◽  
Jon Erickson ◽  
Yu-Chong Tai ◽  
Jerry Pine
Keyword(s):  
Neural Networks ◽  
Survival Rate ◽  
Cell Survival ◽  
Action Potentials ◽  
Fabrication Process ◽  
Glass Substrates ◽  
Cell Survival Rate ◽  
Potential Recording

ABSTRACTParylene neurocages are biocompatible and very robust, making them ideally suited for studying neural networks. We present a design and fabrication process for building parylene neurocages for in vitro studies of neural networks. The fabrication process, on either silicon or glass substrates, incorporates electrodes into the neurocages to allow for stimulation and recording of action potentials. The resulting neurocages have a long-term cell survival rate of ∼50% and have proven to be 99% effective in trapping neurons.

Modified Gelatin-Based Cell Assembling Process Using Glycerin

2012 ◽  
Vol 476-478 ◽  
pp. 443-447
Author(s):  
Hai Xia Liu ◽  
Sheng Jie Li ◽  
Feng Lin ◽  
Yong Nian Yan
Keyword(s):  
Composite Materials ◽  
Survival Rate ◽  
Cell Survival ◽  
Cell Damage ◽  
Cell Assembly ◽  
Assembly Process ◽  
Cell Survival Rate ◽  
Assembly Technology ◽  
Cell Assembling

Cell assembly technology adopting the gelatin-based composite materials has found broad application in the field of disease mechanism research, drug development and organ reconstruction etc. But there are still several troublesome problems, such as the necessaries of high forming concentration of gelatin-based materials and the cell damage produced during extrusion. In view of existing situation, a modified gelatin-based cell assembling process using glycerin was brought forward. The results showed that adding 10% (v/v) glycerin to the existing gelatin-based composite materials, the cells inactivation effect under 4 °C or lower temperature environment can be reduced obviously, meanwhile, the glycerin has a compensatory effect of gelatin. It can significantly improve the forming temperature and the cell survival rate, get high cell survival rate even when the scanning speed is on 40 mm/s. In addition, the glycerin is easier to dissolve in culture medium in the tissue analog training process; it is more conducive to the rapid materials degradation, as well as cell proliferation and tissue regeneration. Therefore, modified gelatin-based cell assembly process with glycerin will be more widely used in tissue or organ in vitro assembly process.

scholarly journals Establishing a Surgical Procedure for Rhesus Epiretinal Scaffold Implantation with HiPSC-Derived Retinal Progenitors

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ziming Luo ◽  
Kang Li ◽  
Kaijing Li ◽  
Bikun Xian ◽  
Ying Liu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Cell Survival ◽  
Surgical Procedure ◽  
Cell Counting ◽  
Fundus Photography ◽  
Cell Survival Rate ◽  
Catheter Group ◽  
Time Required

Background. To develop an effective surgical procedure for cellular scaffold epiretinal implantation in rhesus, facilitating subsequent epiretinal stem cell transplantation. Methods. Retinal progenitors were seeded onto a poly(lactic-co-glycolic) acid (PLGA) scaffold. First, the cellular scaffolds were delivered by 18G catheter or retinal forceps into rabbit epiretinal space (n=50). Then, the cell survival rate was evaluated by Cell Counting Kit-8 (CCK-8). Second, three methods of scaffold fixation, including adhesion after gas-liquid exchange (n=1), tamponade by hydrogel (n=1), and fixation by retinal tacks (n=4), were performed in rhesus monkeys. After one month, fundus photography and SD-OCT were performed to assess the outcomes, and histological examination was performed to evaluate proliferation. Results. The cell survival rate was significantly higher in the catheter group. Follow-up examination showed that retinal tack fixation was the only method to maintain the scaffolds attached to host retina for at least 3 weeks, which is the minimal time required for cell integration. Histological staining demonstrated slight glial fibrillary acidic protein (GFAP) accumulation in the retinal tack insertion area. Conclusions. The established surgical procedure offers a new insight into research of epiretinal cell replacement therapy in rhesus eyes. The successful delivery and long-term fixation provide a prerequisite for cell migration and integration.

scholarly journals Efficient Cryopreservation of Populus tremula by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Elena O. Vidyagina ◽  
Nikolay N. Kharchenko ◽  
Konstantin A. Shestibratov
Keyword(s):  
Survival Rate ◽  
Axillary Buds ◽  
Long Term Storage ◽  
Average Survival ◽  
Transgenic Lines ◽  
Ssr Loci ◽  
One Step ◽  
The One

Axillary buds of in vitro microshoots were successfully frozen at –196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from the point of their influence towards recovery after cryopreservation. It was found out that the use of axillary buds as explants after removal of the apical one increases recovery on average by 8%. The cultivation on growth medium of higher density insignificantly raises the regenerants survival rate. Pretreatment of the osmotic fluid (OF) shows the greatest influence on the survival rate. It leads to the increase in survival rate by 20%. The cryopreservation technology providing regenerants average survival rate of 83% was developed. It was based on the experimental results obtained with explant pretreatment. Incubation time in liquid nitrogen did not affect the explants survival rate after thawing. After six months cryostorage of samples their genetic variability was analyzed. Six variable simple sequence repeat (SSR) loci were used to analyze genotype variability after the freezing-thawing procedure. The microsatellite analysis showed the genetic status identity of plants after cryopreservation and of the original genotypes. The presence of the recombinant gene in the transgenic lines after cryostorage were confirmed so as the interclonal variation in the growth rate under greenhouse conditions. The developed technique is recommended for long-term storage of various breeding and genetically modified lines of aspen plants, as it provides a high percentage of explants survival with no changes in genotype.

A naphthalimide derivative can release COS and form H2S in a light-controlled manner and protect cells against ROS with real-time monitoring ability

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3878-3884
Author(s):  
Wuyang Hua ◽  
Jian Zhao ◽  
Shaohua Gou
Keyword(s):  
Survival Rate ◽  
Cell Survival ◽  
Real Time ◽  
Uv Light ◽  
Real Time Monitoring ◽  
Cell Survival Rate

Triggered by UV light, the donor could release H2S to protect cells against the damage of ROS and prompt the cell survival rate, meanwhile turning on its fluorescence to be monitored in real time.

scholarly journals Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The “Ring Pump”

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 447 ◽  
Author(s):  
Kaoru Uesugi ◽  
Keizo Nishiyama ◽  
Koki Hirai ◽  
Hiroaki Inoue ◽  
Yoichi Sakurai ◽  
...  
Keyword(s):  
Survival Rate ◽  
Cell Survival ◽  
Saline Solution ◽  
Mechanical Load ◽  
Survival Rates ◽  
Cell Culture System ◽  
Large Size ◽  
Cell Survival Rate ◽  
Peristaltic Pumps ◽  
Phosphate Buffered Saline

A ring pump (RP) is a useful tool for microchannels and automated cell culturing. We have been developing RPs (a full-press ring pump, FRP; and a mid-press ring pump, MRP). However, damage to cells which were sent by the RP and the MRP was not investigated, and no other studies have compared the damage to cells between RPs and peristaltic pumps (PPs). Therefore, first, we evaluated the damage to cells that were sent by a small size FRP (s-FRP) and small size MRPs (s-MRPs; gap = 25 or 50 μm, respectively). “Small size” means that the s-FRP and the s-MRPs are suitable for microchannel-scale applications. The survival rate of cells sent by the s-MRPs was higher than those sent by the s-FRP, and less damage caused by the former. Second, we compared the survival rate of cells that were sent by a large size FRP (l-FRP), a large size MRP (l-MRP) (gap = 50 μm) and a PP. “Large size” means that the l-FRP and the l-MRP are suitable for automated cell culture system applications. We could not confirm any differences among the cell survival rates. On the other hand, when cells suspended in Dulbecco’s phosphate-buffered saline solution were circulated with the l-MRP (gap = 50 μm) and the PP, we confirmed a difference in cell survival rate, and less damage caused by the former.

Comparison of Effects of Curcumin and Nano-curcumin on the Survival of Human-Derived Mesenchymal Stem Cells: An Experimental Study

2020 ◽  
Vol 11 (2) ◽  
pp. 148-155
Author(s):  
Pinjari Hameeda ◽  
Sandeep Katti ◽  
Rajkishore Jammalamadugu ◽  
Kishore Bhatt ◽  
Malleswara Rao Peram ◽  
...  
Keyword(s):  
Stem Cells ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Survival Rate ◽  
Cell Viability ◽  
Cell Survival ◽  
Dependent Manner ◽  
Cell Survival Rate ◽  
Post Hoc ◽  
Dose Dependent

Aim: To evaluate and compare the effect of curcumin (CUR) and Nano-curcumin (N-CUR) on human-derived mesenchymal stem cells (MSCs) in a dose-dependent manner. Materials and Methods: An experimental study performed with putative MSCs from a total of five systemically healthy subjects with chronic periodontitis. These putative MSCs were isolated by cell culture and were further characterized and identified by colony-forming unit assay and immunocytochemical analysis using cell surface markers CD105, CD146, CD45 and CD73. The identified MSCs were treated with different doses of CUR and N-CUR, and compared with α-minimum essential medium (α -MEM) for its cell viability by performing MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay for 48 and 72 hr. The statistically analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test and Bonferroni’s post hoc test. Results: Compared to the α-MEM group, both CUR and N-CUR treated cells have shown significantly ( P = .029) higher survival rate at lower concentration (0.1 and 0.5 µM/L), at 48 hr incubation. However, there was no statistically significant difference between the CUR and N-CUR groups on cell survival rate at both 48 and 72 hr incubation. When compared between the concentrations of the same group, significantly higher cell viability ( P = .001) was observed at lower concentrations (0.1, 0.5 µM/L) in both test groups after incubation for 48 and 72 hr. Conclusion: Both CUR and N-CUR have a dose-dependent effect on human derived MSCs survival when incubated for 48 hr, whereas N-CUR shows increased cell survival rate even at 72 hr of incubation. Although, the cautious use of CUR and N-CUR at higher concentrations is recommended.

Synergistic Suppression of Human Multiple Myeloma Cell Growth by the Natural Product TBL-12 in Combination with Low Doses of Velcade: Insight Into Mechanisms

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 5109-5109
Author(s):  
Bhagavathi A Narayanan ◽  
Amitabha Mazumder
Keyword(s):  
Multiple Myeloma ◽  
Survival Rate ◽  
Side Effects ◽  
Cell Growth ◽  
Cell Survival ◽  
Treatment Efficacy ◽  
Low Doses ◽  
Myeloma Cells ◽  
Cell Survival Rate ◽  
Isobologram Analysis

Abstract Abstract 5109 Targeting Multiple Myeloma (MM) cells with potential natural agents could overcome the side effects of current treatment drugs while increasing the efficacy at very low doses. In this study we tested the efficacy of proteasome inhibitor Velcade (Bortezomib) with TBL-12, (an extract from Sea Cucumber, Unicorn Pacific Corporation) in human myeloma cells. Based on the findings from cell survival and proliferation assays we believe that as a single agent TBL-12 is effective in inducing cell growth inhibition with a dose of 100ug/ml in myeloma cells MM1, U266 and ARP1 cells and 200ug/ml in KMSI cells. We further examined the combined effect of Velcade with TBL-12 to test the hypothesis that a natural agent in combination with a pharmaceutical drug at low doses (but with different modes of action) can reduce toxicity while enhancing the treatment efficacy that may increase the survival rate. In this study we report the in vitro effect of low doses of Velcade in combination with TBL-12. We performed cell survival assays in MM1 and U266 cells using very low doses of Valcade ranging from 1–10ngs/ml in combination with an already established dose for TBL-12 (100ugs/ml) showed a time and dose dependent inhibitory effect on cell growth. We observed a cell survival rate that was reduced from 100 % to 30% at 48h and 20% at 72h (p<0.001) in both MM1 and U266 cells. These findings suggest the possibility of using very low doses of Velcade in combination with TBL-12 to be more effective and reduce the toxicity. Mechanistically, stimulation of MM cells with TNFa could trigger the activation of IL-6 and vasculendothelial growth factor (VEGF) and its receptors which will lead to progressive angiogenesis in preclinical models for myeloma. To address the effect of TBL-12 on angiogenesis, we conducted several independent assays by stimulating MM and U266 cells with TNFa or IL-6 for duration of 8h. First we determined the rate of cell survival in MM1 and U266 cells at different time points of 24, 48 and 72h in cells stimulated with TNFa (5ng/ml) followed by treatment with TBL-12 (100ug/ml) alone and in combination with Velcade. Although our data showed a 50% decrease in the cell survival rate by TBL-12 alone, we observed a significant decrease (70–80%, p<0.001)) in the cell survival rate in combination with Velcade in TNFa stimulated cells compared to the control. Co-culturing of myeloma cells MM1 and U266 with human umbilical vein endothelial cells (HUVEC) followed by treatment with an already established dose of TBL-12 showed a significant decrease (45%) in cells adhering to the surface of HUVEC determined by phase contrast and immunofluorescence microscopic observations. These findings suggest that TBL-12 may have potential to inhibit angiogenesis by targeting VEGF receptors in combination with Velcade at low doses (determined by isobologram analysis). Evidence from Western blot analysis of the MM1 and U266 cells treated with TBL-12 indicates a significant accumulation of caspase -3 and caspase -9 indicating the underlying targets of TBL-12 in mediating apoptosis. Findings on the isobologram analysis indicating synergistic effects exerted by TBL-12 in combination with Velcade on VEGFR1 receptor, and modulation of Bcl2 and Bax that is associated with apoptosis will be discussed during presentation. Overall findings from this study suggest the potential use of TBL-12 in combination with Velcade could improve treatment efficacy with reduced side effects related to high dose toxicity. Currently we are doing trials with TBL-12 at NYUCI and this data could form the basis for future trials. Disclosures: No relevant conflicts of interest to declare.

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