Cellular Adhesion and Spreading of Endothelial Cells Monitored in Real Time Using the Quartz Crystal Microbalance

1998 ◽  
Vol 550 ◽  
Author(s):  
Tiean Zhou ◽  
Susan J. Braunhut ◽  
Diane Medeiros ◽  
Kenneth A. Marx
Keyword(s):  
Endothelial Cells ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Cell Mass ◽  
Viscoelastic Behavior ◽  
Cellular Adhesion ◽  
Frequency Change ◽  
Resistance Change ◽  
Continuous Increase ◽  
Mock Cell

AbstractWe have applied the Quartz Crystal Microbalance (QCM) technique to continuously record the processes of endothelial cell (EC) adhesion, spreading and cellular mass distribution changes during initial cell to surface contact and homeostatic attachment. ECs (50,000) were layered onto a set volume of media in the QCM device and simultaneously in a mock cell used for photomicroscopy. As cells were observed in the mock cell device to contact and attach to the surface over 45-55 min, we measured in the QCM device a continuous decrease in frequency and continuous increase in resistance, achieving a maximum at about one hr (1400 Ω frequency change and 1400 Ω motional resistance change). These frequency and resistance values stabilized over the next 24 hrs and were unchanged out to 72 hr by QCM measurement (to ∼700 Hz, ∼700 Ω), as the cells were observed to spread in the mock device. Both bovine aortic (BAE) and bovine capillary (BCE) endothelial cells were studied and found to exhibit similar behavior. These studies demonstrate that QCM can be used to detect continuous changes in cell mass and viscoelastic behavior.

Charge Transfer Property of Self-Assembled Viologen Derivative by Electrochemical Quartz Crystal Microbalance Response

2006 ◽  
Vol 6 (11) ◽  
pp. 3657-3660 ◽  
Author(s):  
Dong-Yun Lee ◽  
A. K. M. Kafi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon
Keyword(s):  
Charge Transfer ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Modified Electrodes ◽  
Electrochemical Quartz Crystal Microbalance ◽  
Frequency Change ◽  
Self Assembled Monolayer ◽  
Transfer Property ◽  
Redox Peak ◽  
Self Assembled

Viologen modified electrodes have been extensively investigated with quartz crystal microbalance (QCM), which has been known as a nano-gram order mass detector, because of their highly reversible electrochemical properties, especially the first reduction-oxidation cycle of V2+ ↔ V•+. The purpose of this work was to study the charge transfer characteristics of self-assembled monolayer (SAM) by changing electrolyte solutions where the cations and anions are different. The redox peak currents were nearly equal charges during redox processes and showed an excellent linear interrelation between the scan rates and second redox peak currents. The charge transfer of self-assembled viologen monolayer was determined by the mass change during the cyclic voltammetry (CV). The total frequency change was about 17.8 Hz, 19.6 Hz, 9.5 Hz, and 8.4 Hz. From this data, we could know the transferred mass was about 19.0 ng, 20.9 ng, 10.2 ng, and 9.0 ng. Finally, the electrochemical quartz crystal microbalance (EQCM) has been employed to monitor the electrochemically induced adsorption of self-assembled monolayer.

scholarly journals Effect of Ionic Strength on Initial Interactions ofEscherichia coli with Surfaces, Studied On-Line by a Novel Quartz Crystal Microbalance Technique

1999 ◽  
Vol 181 (17) ◽  
pp. 5210-5218 ◽  
Author(s):  
Karen Otto ◽  
Hans Elwing ◽  
Malte Hermansson
Keyword(s):  
Ionic Strength ◽  
Quartz Crystal Microbalance ◽  
Direct Contact ◽  
Quartz Crystal ◽  
Contact Point ◽  
Viscoelastic Behavior ◽  
Bacterial Cells ◽  
Hydrophobic Surfaces ◽  
Intimate Contact ◽  
Cell Surface Structures

ABSTRACT A novel quartz crystal microbalance (QCM) technique was used to study the adhesion of nonfimbriated and fimbriated Escherichia coli mutant strains to hydrophilic and hydrophobic surfaces at different ionic strengths. This technique enabled us to measure both frequency shifts (Δf), i.e., the increase in mass on the surface, and dissipation shifts (ΔD), i.e., the viscoelastic energy losses on the surface. Changes in the parameters measured by the extended QCM technique reflect the dynamic character of the adhesion process. We were able to show clear differences in the viscoelastic behavior of fimbriated and nonfimbriated cells attached to surfaces. The interactions between bacterial cells and quartz crystal surfaces at various ionic strengths followed different trends, depending on the cell surface structures in direct contact with the surface. While Δf and ΔD per attached cell increased for nonfimbriated cells with increasing ionic strengths (particularly on hydrophobic surfaces), the adhesion of the fimbriated strain caused only low-level frequency and dissipation shifts on both kinds of surfaces at all ionic strengths tested. We propose that nonfimbriated cells may get better contact with increasing ionic strengths due to an increased area of contact between the cell and the surface, whereas fimbriated cells seem to have a flexible contact with the surface at all ionic strengths tested. The area of contact between fimbriated cells and the surface does not increase with increasing ionic strengths, but on hydrophobic surfaces each contact point seems to contribute relatively more to the total energy loss. Independent of ionic strength, attached cells undergo time-dependent interactions with the surface leading to increased contact area and viscoelastic losses per cell, which may be due to the establishment of a more intimate contact between the cell and the surface. Hence, the extended QCM technique provides new qualitative information about the direct contact of bacterial cells to surfaces and the adhesion mechanisms involved.

scholarly journals Response of Quartz Crystal Microbalance Loaded with Single-drop Liquid in Gas Phase

2014 ◽  
Vol 8 (1) ◽  
pp. 197-201
Author(s):  
Li Yang ◽  
Xianhe Huang
Keyword(s):  
Resonant Frequency ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Liquid Droplet ◽  
Theoretical Formula ◽  
Frequency Change ◽  
Single Drop ◽  
The Relationship ◽  
Liquid Property ◽  
Repeated Experiment

The frequency response of quartz crystal microbalance loaded by single-drop liquid is studied in this paper. Previous studies have shown that the relationship between resonant frequency and properties of liquid by completely immersing one side of the crystal in liquid. In this work, only localized portion of crystal was wetted by liquid droplet. Repeated experiment shows the relationship between liquid property include viscosity and density to resonant frequency. Furthermore, Theoretical formula describing the frequency change of the quartz crystal microbalance with liquid property is proposed. The predicted results showed distinct coincide with experimental results.

Modification of Polystyrene Morphology and its Influence to the Coated Zinc Phthalocyanine Layer and Frequency Change in QCM Sensor

2015 ◽  
Vol 827 ◽  
pp. 257-261 ◽  
Author(s):  
Masruroh ◽  
D.J.D.H. Djoko ◽  
Lalu A. Didik ◽  
Eka Rachmawati ◽  
Fadli Robiandi ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Quartz Crystal Microbalance ◽  
Bovine Serum ◽  
Quartz Crystal ◽  
Zinc Phthalocyanine ◽  
Frequency Change ◽  
Polystyrene Surface ◽  
Influence Of Solvents

This study observed the influence of solvents on polystyrene regarding the layered morphologies of zinc phthalocyanine (ZnPc) and its influence on the bovine serum albumin (BSA) immobilization on quartz crystal microbalance (QCM) sensor. The larger surface width introduced by surface roughness of the polystyrene due to chloroform (CHCl3) as the solvents allows more ZnPc to be trapped within the polystyrene. Moreover, the wider surface width also increases the amount of ZnPc to be deposited on top of the polystyrene surface. Therefore, the ZnPc layer deposited onto polystyrene created with CHCl3 solvent is thicker than other solvents, which result in the largest ∆f of the ZnPc layer.

Frequency change of a quartz crystal microbalance at the supercritical condition of carbon dioxide

1994 ◽  
Vol 7 (4) ◽  
pp. 289-292 ◽  
Author(s):  
Katsuto Otake ◽  
Shigeru Kurosawa ◽  
Takeshi Sako ◽  
Tsutomu Sugeta ◽  
Masaru Hongo ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Supercritical Condition ◽  
Frequency Change

Immobilization, Hybridization and Amperometric Detection of DNA Sensor Using Quartz Crystal Microbalance

2008 ◽  
Vol 8 (9) ◽  
pp. 4553-4556 ◽  
Author(s):  
Dong-Yun Lee ◽  
Won-Suk Choi ◽  
A. K. M. Kafi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon
Keyword(s):  
Detection Limit ◽  
Quartz Crystal Microbalance ◽  
Gold Electrode ◽  
Quartz Crystal ◽  
Supporting Electrolyte ◽  
Amperometric Detection ◽  
Frequency Change ◽  
Dna Sensor ◽  
Anodic Peak Current ◽  
Over Time

A synthesized 21-mer single-stranded DNA (ssDNA) was covalently immobilized onto a self-assembled aminoethanethiol monolayer modified gold electrode onto quartz crystal microbalance (QCM). The covalently immobilized ssDNA was hybridized with complementary ssDNA. The interaction between surface-immobilized ssDNA and complementary 21-mer DNA in solution was also examined. Each step was followed by monitoring changes in the QCM frequency over time. From the results, the measured frequency change was about 256 Hz, 160 Hz and 141 Hz, respectively. As a result, the adsorption mass was about 273 ng/cm2, 171 ng/cm2 and 151 ng/cm2, according to the Sauerbrey equation. Also, PBS with pH 7.0 was selected as a supporting electrolyte to achieve maximum sensitivity and good bioactivity. The anodic peak current was linearly related to the concentrations between 1 × 10−5 and 9 × 10−5 M with a detection limit of 1.0 × 10 −6 M.

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