Using Indentation and Intra-Vascular Ultrasound to Measure Arterial Response

2000 ◽  
Vol 662 ◽  
Author(s):  
P.M. Anderson ◽  
E.N. Glaser ◽  
A.I. Veress ◽  
G.M. Pharr ◽  
D.G. Vince ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Contact Point ◽  
Accurate Determination ◽  
Initial Contact ◽  
Vascular Ultrasound ◽  
Internal Dissipation ◽  
Microindentation Technique ◽  
Micro Indentation

AbstractTwo alternatives to standard tensile testing of arteries are discussed. The first involves inflation of arteries and simultaneous measurement of radial displacement with intra-vascular ultrasound (IVUS). The second involves the measurement of load versus displacement during micro-indentation of the intimal surface. The IVUS technique is used to study the nonlinear stiffening of porcine coronaries during inflation and, ultimately, it may provide a method to determine mechanical properties in vivo. Processing of the IVUS data relies on accurate determination of the luminal/intimal and medial/advential boundaries during inflation. The microindentation technique is used to study the effect of loading rate on tissue stiffness, recovery, and internal dissipation. Ultimately, this technique may provide a method to measure local mechanical properties in the vicinity of an atherosclerotic plaque, for example. Accurate determination of the initial contact point between the indenter and intima is required, however. The techniques appear to successfully capture significant, nonlinear, time-dependent properties of arterial tissue.

scholarly journals Harnessing PET to track micro- and nanoplastics in vivo

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Outi Keinänen ◽  
Eric J. Dayts ◽  
Cindy Rodriguez ◽  
Samantha M. Sarrett ◽  
James M. Brennan ◽  
...  
Keyword(s):  
Sea Water ◽  
Accurate Determination ◽  
Nuclear Imaging ◽  
Imaging Data ◽  
Biodistribution Studies ◽  
Positron Emission ◽  
20 Nm ◽  
Pharmacokinetic Behavior

AbstractThe proliferation of plastics in the environment continues at an alarming rate. Plastic particles have been found to be persistent and ubiquitous pollutants in a variety of environments, including sea water, fresh water, soil, and air. In light of this phenomenon, the scientific and medical communities have become increasingly wary of the dangers posed to human health by chronic exposure to microplastics (< 5 mm diameter) and nanoplastics (< 100 nm diameter). A critical component of the study of the health effects of these pollutants is the accurate determination of their pharmacokinetic behavior in vivo. Herein, we report the first use of molecular imaging to track polystyrene (PS) micro- and nanoplastic particles in mammals. To this end, we have modified PS particles of several sizes—diameters of 20 nm, 220 nm, 1 µm, and 6 µm—with the chelator desferrioxamine (DFO) and radiolabeled these DFO-bearing particles with the positron-emitting radiometal zirconium-89 (89Zr; t1/2 ~ 3.3 d). Subsequently, positron emission tomography (PET) was used to visualize the biodistribution of these radioplastics in C57BL/6J mice at 6, 12, 24, and 48 h after ingestion. The imaging data reveal that the majority of the radioplastics remain in the gastrointestinal tract and are eliminated through the feces by 48 h post-ingestion, a result reinforced by acute biodistribution studies. Ultimately, this work suggests that nuclear imaging—and PET in particular—can be a sensitive and effective tool in the urgent and rapidly growing effort to study the in vivo behavior and potential toxicity of micro- and nanoplastics.

Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid–liquid microextraction by UHPLC-MS/MS

RSC Advances ◽  
2016 ◽  
Vol 6 (110) ◽  
pp. 108635-108644 ◽  
Author(s):  
Xian-En Zhao ◽  
Yongrui He ◽  
Ping Yan ◽  
Na Wei ◽  
Renjun Wang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Rat Brain ◽  
Accurate Determination ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

In situ UA-DDLLME coupled with UHPLC-MS/MS has been developed for simultaneous determination of neurotransmitters and baicalein from Parkinson's disease rats.

Determination of mechanical properties of the weld line by combining micro-indentation with inverse modeling

2014 ◽  
Vol 85 ◽  
pp. 347-362 ◽  
Author(s):  
Guangyong Sun ◽  
Fengxiang Xu ◽  
Guangyao Li ◽  
Xiaodong Huang ◽  
Qing Li
Keyword(s):  
Mechanical Properties ◽  
Inverse Modeling ◽  
Weld Line ◽  
Micro Indentation

Evaluation of Micro-Pillar Compression Tests for Accurate Determination of Elastic-Plastic Constitutive Relations

2012 ◽  
Vol 79 (6) ◽  
Author(s):  
Huiyang Fei ◽  
Amit Abraham ◽  
Nikhilesh Chawla ◽  
Hanqing Jiang
Keyword(s):  
Mechanical Properties ◽  
Finite Element Analysis ◽  
Compression Test ◽  
Constitutive Relations ◽  
Accurate Determination ◽  
Compression Tests ◽  
Element Analysis ◽  
Plastic Materials ◽  
Properties Of Materials

The micro-pillar compression test is emerging as a novel way to measure the mechanical properties of materials. In this paper, we systematically conducted finite element analysis to evaluate the capability of using a micro-compression test to probe the mechanical properties of both elastic and plastic materials. We found that this test can provide an alternative way to accurately and robustly measure strain, and to some extent, stress. Therefore, this test can be used to measure some strain related quantities, such as strain to failure, or the stress-strain relations for plastic materials.

La formation des liaisons éthériques lors de la production du polytéréphthalate d'éthylène glycol

1978 ◽  
Vol 56 (21) ◽  
pp. 2703-2706 ◽  
Author(s):  
Vladimir Hornof ◽  
Leoš Zeman
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Ethylene Glycol ◽  
Chemical Method ◽  
Periodic Acid ◽  
Accurate Determination ◽  
Physical And Mechanical Properties ◽  
Resistance To Oxidation ◽  
By Products

Ether-type linkages are formed as by-products during the preparation of polyethylene terephthalate from dimethyl terephthalate and ethylene glycol. Incorporated in the polymer, these linkages affect its physical and mechanical properties such as tensile strength, melting point, and resistance to oxidation. An improved chemical method of analysis has been developed allowing an accurate determination of ether bonds in polyester fibres. The method is based on the selective oxidation of ethylene glycol with periodic acid and takes advantage of hydrolysis with water under pressure to decompose the polymer. The method has been employed to study the effect of the concentration of polycondensation catalyst on the formation of ethers.

scholarly journals Numerical Model for the Determination of Erythrocyte Mechanical Properties and Wall Shear Stress in vivo From Intravital Microscopy

2020 ◽  
Vol 10 ◽  
Author(s):  
Vivek P. Jani ◽  
Alfredo Lucas ◽  
Vinay P. Jani ◽  
Carlos Munoz ◽  
Alexander T. Williams ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Shear Stress ◽  
Numerical Model ◽  
Wall Shear Stress ◽  
Intravital Microscopy ◽  
Wall Shear

Use of 13C-labeled glucose for estimating glucose oxidation: some design considerations

1987 ◽  
Vol 63 (5) ◽  
pp. 1725-1732 ◽  
Author(s):  
J. J. Robert ◽  
J. Koziet ◽  
D. Chauvet ◽  
D. Darmaun ◽  
J. F. Desjeux ◽  
...  
Keyword(s):  
Glucose Oxidation ◽  
Accurate Determination ◽  
Metabolic States ◽  
Series Of Experiments ◽  
Time Required ◽  
Infusion Protocol ◽  
The Ideal ◽  
Expired Air

Use of 13C-labeled glucose for estimating in vivo rates of glucose oxidation faces several difficulties, particularly the accurate determination of the output of 13C in expired air. In an investigation of wholebody glucose metabolism in healthy adult humans, using a continuous intravenous infusion of D-[U-13C]glucose, we found that a precise estimate of the rate of glucose oxidation was difficult to achieve when the study included infusions with unlabeled glucose. Problems arose 1) as a result of the slow rate at which the 13CO2 released by glucose oxidation reaches an equilibrium in expired air CO2 and 2) due to the contribution to 13CO2 output by the natural 13C in the unlabeled glucose that was infused. In a subsequent series of experiments in healthy young adults, we found that the entry of 13CO2 released by the tissues into the bicarbonate pool and into the expired air is relatively slow and a tracer infusion protocol of approximately 6 h is required for determination of glucose oxidation. This applies when metabolic states are changed acutely during the experiment or when unlabeled glucose is infused. However, for resting subjects in the basal postabsorptive state we confirmed that the time required to achieve a steady state in the 13C enrichment of expired air can be shortened significantly by the use of a NaH13CO3 priming dose, even when this dose varies from the ideal.

scholarly journals Targeted LC-MS/MS platform for the comprehensive determination of peptides in the kallikrein-kinin system

2021 ◽  
Author(s):  
Tanja Gangnus ◽  
Bjoern B. Burckhardt
Keyword(s):  
Healthy Volunteers ◽  
Clinical Symptoms ◽  
Matrix Effects ◽  
Accurate Determination ◽  
Kinin System ◽  
Carry Over ◽  
Kallikrein Kinin System ◽  
The Impact

AbstractThe kallikrein-kinin system (KKS) is involved in many physiological and pathophysiological processes and is assumed to be connected to the development of clinical symptoms of angioedema or COVID-19, among other diseases. However, despite its diverse role in the regulation of physiological and pathophysiological functions, knowledge about the KKS in vivo remains limited. The short half-lives of kinins, their low abundance and structural similarities and the artificial generation of the kinin bradykinin greatly hinder reliable and accurate determination of kinin levels in plasma. To address these issues, a sensitive LC-MS/MS platform for the comprehensive and simultaneous determination of the four active kinins bradykinin, kallidin, des-Arg(9)-bradykinin and des-Arg(10)-kallidin and their major metabolites bradykinin 2-9, bradykinin 1-7 and bradykinin 1-5 was developed. This platform was validated according to the bioanalytical guideline of the US Food and Drug Administration regarding linearity, accuracy, precision, sensitivity, carry-over, recovery, parallelism, matrix effects and stability in plasma of healthy volunteers. The validated platform encompassed a broad calibration curve range from 2.0–15.3 pg/mL (depending on the kinin) up to 1000 pg/mL, covering the expected concentrations in disease states. No source-dependent matrix effects were identified, and suitable stability of the analytes in plasma was observed. The applicability of the developed platform was proven by the determination of endogenous levels in healthy volunteers, whose plasma kinin levels were successfully detected in the low pg/mL range. The established platform facilitates the investigation of kinin-mediated diseases (e.g. angioedema, COVID-19) and enables the assessment of the impact of altered enzyme activities on the formation or degradation of kinins. Graphical abstract

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