Electrochemical detection of non-labeling DNA using electronic array

2002 ◽  
Vol 761 ◽  
Author(s):  
H. Y. Lee ◽  
J. W. Park ◽  
Y. S. Choi ◽  
T. Kannno ◽  
Hiro. Tanaka ◽  
...  
Keyword(s):  
Electron Transport ◽  
Nucleic Acids ◽  
Electrochemical Detection ◽  
Dna Hybridization ◽  
Detection System ◽  
Good Reproducibility ◽  
Redox Peak ◽  
Target Dna ◽  
Redox Peak Current ◽  
Hybridization Detection

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 ?M concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.

Electrochemical detection of non-labeling DNA using electronic array

2002 ◽  
Vol 735 ◽  
Author(s):  
H. Y. Lee ◽  
J. W. Park ◽  
Y. S. Choi ◽  
T. Kannno ◽  
Hiro. Tanaka ◽  
...  
Keyword(s):  
Electron Transport ◽  
Nucleic Acids ◽  
Electrochemical Detection ◽  
Dna Hybridization ◽  
Detection System ◽  
Good Reproducibility ◽  
Redox Peak ◽  
Target Dna ◽  
Redox Peak Current ◽  
Hybridization Detection

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 μM concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.

scholarly journals Substrate Optimization of Carbon Nanomaterial Based DNA Hybridization Detection System

2018 ◽  
Vol 114 (3) ◽  
pp. 686a
Author(s):  
Sethan K. Jasti ◽  
Shawn M. McGinley ◽  
Franzel Pena ◽  
Samuel Opper ◽  
Ewa S. Kirkor ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Detection System ◽  
Carbon Nanomaterial ◽  
Hybridization Detection

Development of an electrochemical DNA biosensor based on Quercetin as a new electroactive indicator for DNA hybridization detection

2021 ◽  
Author(s):  
Esmaeel Alipour ◽  
Sheida Norouzi ◽  
Shokoufe Moradi
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Dna Hybridization ◽  
Electrode Surface ◽  
Dna Biosensor ◽  
Carbon Electrode ◽  
Electrochemical Dna Biosensor ◽  
Specific Target ◽  
Target Dna ◽  
Hybridization Detection

Electrochemical DNA biosensor is designed for detection of specific target DNA after hybridization with complementary probe DNA immobilized onto glassy carbon electrode surface. Quercetin was successfully used as a new...

A DNA hybridization detection sensor based on photo biased ZnO thin film FET devices

Sensor Review ◽  
2016 ◽  
Vol 36 (4) ◽  
pp. 368-376
Author(s):  
Mehdi Habibi ◽  
Maryam Fanaei
Keyword(s):  
Dna Hybridization ◽  
Noise Sources ◽  
Charge Detection ◽  
Content Type ◽  
Target Dna ◽  
Fabrication Procedure ◽  
Effect Transistor ◽  
Measurement Devices ◽  
A Charge ◽  
Hybridization Detection

Purpose The purpose of this paper is to present a DNA hybridization detection sensor. An inexpensive fabrication procedure was used so that the sensors can be disposed economically after the measurement is completed. Design/methodology/approach Field effect transistor (FET) devices are used in the proposed structure. The FET device acts as a charge detection element and produces an amplified output current based on surface charge variations. As amplification is performed directly at the sensor frontend, noise sources have less effect on the detected signal, and thus, acceptably low DNA concentrations can be detected with simple external electronics. ZnO nano layers are used as the FET active semiconductor channel. Furthermore, a photobiasing approach is used to adjust the operating point of the proposed FET without the need for an additional gate terminal. Findings The proposed sensor is evaluated by applying matched and unmatched target DNA fragments on the fabricated sensors with capture probes assembled either directly on the ZnO surface or on a nano-platinum linker layer. It is observed that the presented approach can successfully detect DNA hybridization at the nano mole range with no need for complex laboratory measurement devices. Originality/value The presented photobiasing approach is effective in the adjustment of the sensor sensitivity and decreases the fabrication complexity of the achieved sensor compared with previous works.

scholarly journals DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dezhi Feng ◽  
Jing Su ◽  
Yi Xu ◽  
Guifang He ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Reaction Time ◽  
Electrochemical Detection ◽  
Detection System ◽  
Detection Performance ◽  
Pdms Layer ◽  
Microfluidic Chips ◽  
Serum Samples ◽  
Gold Nanoflowers ◽  
Dna Tetrahedron

AbstractProstate-specific antigen (PSA) is the most widely used biomarker for the early diagnosis of prostate cancer. Existing methods for PSA detection are burdened with some limitations and require improvement. Herein, we developed a novel microfluidic–electrochemical (μFEC) detection system for PSA detection. First, we constructed an electrochemical biosensor based on screen-printed electrodes (SPEs) with modification of gold nanoflowers (Au NFs) and DNA tetrahedron structural probes (TSPs), which showed great detection performance. Second, we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering microchannels. Finally, the μFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection platform. The μFEC system we developed acquired great detection performance for PSA detection in PBS solution. For PSA assays in spiked serum samples of the μFEC system, we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction time <25 min. Real serum samples of prostate cancer patients presented a strong correlation between the “gold-standard” chemiluminescence assays and the μFEC system. In terms of operation procedure, cost, and reaction time, our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.

scholarly journals COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR

1996 ◽  
Vol 42 (12) ◽  
pp. 1915-1923 ◽  
Author(s):  
N DiDomenico ◽  
H Link ◽  
R Knobel ◽  
T Caratsch ◽  
W Weschler ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Internal Control ◽  
Detection System ◽  
Dna Amplification ◽  
Cross Reactivity ◽  
Diagnostic Pcr ◽  
Thermal Cycler ◽  
Paramagnetic Microparticles ◽  
Rna And Dna ◽  
Single Reaction

Abstract The COBAS AMPLICOR system automates amplification and detection of target nucleic acids, making diagnostic PCR routine for a variety of infectious diseases. The system contains a single thermal cycler with two independently regulated heating/cooling blocks, an incubator, a magnetic particle washer, a pipettor, and a photometer. Amplified products are captured on oligonucleotide-coated paramagnetic microparticles and detected with use of an avidin-horseradish peroxidase (HRP) conjugate. Concentrated solutions of amplicon or HRP were pipetted without detectable carryover. Amplified DNA was detected with an intraassay CV of &lt; 4.5%; the combined intraassay CV for amplification and detection was &lt; 15%. No cross-reactivity was observed when three different target nucleic acids were amplified in a single reaction and detected with three target-specific capture probes. The initial COBAS AMPLICOR menu includes qualitative tests for diagnosing infections with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycobacterium tuberculosis, and hepatitis C virus. All tests include an optional Internal Control to provide assurance that specimens are successfully amplified and detected.

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