A new look at endometriosis: the role of restoring of full immune surveillance over ectopic cells of the endometrium

2017 ◽  
pp. 99-106
Author(s):  
S.M. Melnikov ◽  
◽  
O.F. Tatskyy ◽  
Keyword(s):  
Clinical Symptoms ◽  
Immune Surveillance ◽  
Hormonal Treatment ◽  
Regulatory Peptides ◽  
Control Group ◽  
Immune Homeostasis ◽  
Childbearing Age ◽  
Endometrial Cells ◽  
Immunological Disorders

The article deals with the role of immunological disorders in the occurrence of genital endometriosis recurrences, as well as the results of treatment of this disease. The objective: the study of the effect of restoring immune homeostasis with the help of exogenous peptides on the effectiveness of the treatment of endometriosis. Patients and methods. Endometriosis was treated in 68 women of childbearing age. In 48 patients, uterine endometriosis (adenomyosis) was diagnosed, and 20 cases were endometriosis of the vaginal part of the cervix. In order to study the effectiveness of Camelyn-Bio in the treatment of endometriosis, patients were divided into two groups. Results. In the group of patients who were prescribed hormonal monotherapy, 3 months after the end of the course of hormonal treatment, recurrence of endometriosis was observed. They arose due to systemic and local immunological disorders, which probably led to ectopic endometrial cells from immune surveillance. Due to the properties of the exogenous peptides of the Camelyn-Bio preparation, reacting with the regulatory peptides of the ectopic cells of the endometrium, there are conditions for a complete immunological surveillance of the endometrium foci. In the group of patients treated with Camelyn-Bio, the incidence of relapse was 4 times lower than that of the control group. Conclusion. Treatment of endometriosis with the combined progestogen and estragon drug is effective and contributes to a sustained regression of the clinical symptoms of the disease. A break in the use of basic hormonal therapy leads to relapses of endometriosis, which is confirmed by the instrumental methods of research. Clinical experience confirms that the restoration of the immune homeostasis of patients with the Camelyn-Bio preparation is an important factor in increasing the effectiveness of endometriosis treatment, leads to clinical remission and improves the quality of life. Key words: endometriosis, ectopic cells of the endometrium, apoptosis, Camelyn-Bio, immune homeostasis, relapses, treatment.

The role of exogenous peptides in restoration of full immune response under secondary immunodeficiency

2017 ◽  
pp. 89-97
Author(s):  
A.I. Kurchenko ◽  
◽  
V.A. Benyuk ◽  
H.P. Potebnya ◽  
V.L. Kobys ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Combined Therapy ◽  
Cervical Dysplasia ◽  
Control Group ◽  
Immune Homeostasis ◽  
Childbearing Age ◽  
Papillomavirus Infection ◽  
Clinically Significant ◽  
And Control

The objective: defining the role of immunomodulation with the use of exogenous peptides MHP (Camelyn-Bio) on the effectiveness of treatment of cervical dysplasia I-II that was caused by mixed chlamydia and human papillomavirus infection. Patients and methods. In accordance with the objectives of the study we selected and randomized 72 patients with dysplasia (paplomavirus-chlamydial etiology). Women were divided into two groups: basic (n=36) and control (n=36) matched by age, symptoms and duration of the process. The main conventional treatment group received Camelyn-Bio capsules at a dose of 1 capsule (0.19 g) 3 times a day 30 days from starting treatment. Results. The result of the reduction in viral load lower than a clinically significant threshold (3 lg) was achieved in 70% of patients in control group and 77.8% in the main group. Therefore, adequate complex therapy taking into account the role of exogenous peptides reduces the activity of HPV, promotes its elimination, reduces the frequency of relapses. The effectiveness of combined therapy of mild cervical dysplasia with the use of Camelyn-Bio was 88.9%. In the group of patients receiving standard therapy, the efficacy was 83.4%. Conclusion. Now to resolve vital clinical tasks a practitioner has an innovative agent Camelyn-Bio, which, in particular, speeds up the elimination of HPV in women of childbearing age. The inclusion of Camelyn-Bio in the traditional scheme of treatment of cervical dysplasia is justified etiopatogeneticaly and allows to harmonize the immune homeostasis of patients and allows to increase the effectiveness of treatment. Key words: exogenous peptides, T-lymphocytes, macrophages, interleukins, immune homeostasis, human papillomavirus, dysplasia, treatment, efficacy.

ATI-2341 Trifluoroacetic Acid (TFA) Promotes Menstrual Blood-Derived Mesenchymal Stem Cell Recruitment and Enhances Uterine Repair Through Gi Pathway in Asherman’s Syndrome

2022 ◽  
Vol 12 (3) ◽  
pp. 506-513
Author(s):  
Ying Lv ◽  
Liyan Ye ◽  
Xiujuan Zheng
Keyword(s):  
Menstrual Blood ◽  
Control Group ◽  
Dependent Manner ◽  
Asherman’S Syndrome ◽  
Expression Levels ◽  
Endometrial Cells ◽  
Injury Model ◽  
Recruitment Effect ◽  
Dose Dependent

This study aimed to explore the role of ATI-2341 in Asherman’s syndrome and its impact on menstrual blood-derived mesenchymal stem cells (MenSCs). Following establishment of endometrial injury model, MenSCs were extracted from rats and cultured. They were treated with ATI-2341 TFA at different concentrations (10 ng/mL, 50 ng/mL, 100 ng/mL) and MenSCs treated without ATI-2341 TFA were taken as controls. Flow cytometry was conducted to detect the cell cycle. MTT was carried out to evaluate proliferation of endometrial cells. The expression levels of MMP-9, TIMP-1, CK, and VIM were determined with staining used to reflect morphology of endometrium. Administration with ATI-2341 TFA resulted in decreased expression of MMP-9 and increased expression of TIMP-1 in a dose-dependent manner. Of note, the increase of ATI-2341 TFA concentration was accompanied with elevated cell proliferation rate, increased number of glands in the endometrium, and decreased fibrosis area. As treated with 100 ng/mL ATI-2341 TFA, the cells exhibited more glands than that under other concentrations with uniformly arranged glands and lowest expression levels of CK and VIM, control group had plenty of blue-stained collagen fibers in the intima and least amount of glands. ATI-2341 TFA 100 ng/mL induced endometrial epithelial recruitment effect on MenSCs and promoted endometrial repair more significantly than Gi-3 pathway agonists. Collectively, ATI-2341 TFA enhances MenSC recruitment and facilitates endometrial epithelial cells proliferation and the repair of uterine damage in Asherman’s syndrome through Gi pathway. These findings provide a\ novel insight into the MenSC-based treatment against Asherman’s syndrome and deserve further investigation.

scholarly journals Autophagy Suppresses Invasiveness of Endometrial Cells through Reduction of Fascin-1

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaomei Luo ◽  
Wei Cheng ◽  
Shizhang Wang ◽  
Zhihong Chen ◽  
Jieqiong Tan
Keyword(s):  
Metabolic Diseases ◽  
Growth Curves ◽  
Cellular Growth ◽  
Control Group ◽  
Scratch Assay ◽  
Endometrial Cells ◽  
Autophagy Inhibitor ◽  
Exogenous Expression ◽  
Potential Therapeutics

Objective. Autophagy has been reported to be involved in the development of various disorders such as neurodegenerative and metabolic diseases and tumors. Autophagy activators and inhibitors are also potential therapeutics for these diseases. However, the mechanism of autophagic involvement in different diseases is not the same, and the role of autophagy in endometriosis (EM) has not yet been elucidated. This research investigated the mechanism by which autophagy acts in EM, with the aim of establishing a theoretical basis for its prevention and treatment through the targeted interference with autophagy. Methods. We used an RNA interference fragment targeting ATG5, the autophagy activator rapamycin, and the autophagy inhibitor 3-MA or overexpression of filopodia-related protein fascin-1, in conjunction with clonogenic assays, growth curves, and scratch assay to investigate the influence of autophagy on cellular growth, proliferation, and invasiveness. We collected specimens from 20 clinical cases of EM and investigated the protein expression of the autophagic marker LC3-II, the autophagic substrate p62, and fascin-1. Results. Rapamycin was able to inhibit the proliferation and colony formation of the endometriotic cell line CRL-7566, whereas the autophagy inhibitor 3-MA as well as the interference with the autophagy-related gene ATG5 had the opposite effect. More importantly, the autophagy activator rapamycin was able to inhibit the growth of filopodia in the endometriotic cells, and the overexpression of the fascin-1 restored the rapamycin-induced decrease of invasiveness. We found that the expression of the autophagy marker LC3-II was significantly reduced among the clinical EM specimens compared to the control group, while the expressions of fascin-1 and autophagic substrate p62 were increased. Conclusion. Our results indicate that the inhibition of autophagy and exogenous expression of fascin-1 may promote the invasiveness of endometrial cells. As a corollary, autophagy represents a potential target for the treatment of EM.

scholarly journals Association of HMGB1, S100A12 and IL-17A Expression with Immunoglobulin-Resistant in Kawasaki Disease and the Comparisons Between Different Adjunctive Therapeutic Approaches

2022 ◽  
Author(s):  
Yang Zhou ◽  
Liu Pan ◽  
You-jun Yang ◽  
Shi-yu Li ◽  
Wei Yin ◽  
...  
Keyword(s):  
Adjunctive Therapy ◽  
Clinical Symptoms ◽  
Poor Response ◽  
Serum Levels ◽  
Ivig Treatment ◽  
Control Group ◽  
Laboratory Parameters ◽  
Methyl Prednisolone ◽  
Laboratory Variables

Abstract Objective: The DAMPs such as HMGB1, S100A12 and IL-17A have been reported to predict poor response to IVIG. The aim of this study was to analyze the role of HMGB1,S100A12 and IL-17A in the detection of inflammation in KD patients with IVIG-resistant, and to investigate the value of different adjunctive therapy.Method: This study enrolled 126 patients diagnosed with KD, as well as age-matched 16 febrile control subjects. The demographic or clinical data, laboratory parameter and blood sample were collected. Various laboratory parameters as predictive factors for IVIG-resistant were calculated. And the serum levels of IL-17A and mRNA expression levels of HMGB1 and S100A12 were tested in all patients. For patients with acute KD in IVIG-resistant, we studied the levels of laboratory variables when using of IVIG retreatment, methylprednisolone, infliximab for children patients. Result: The variance of laboratory parameters between the febrile control group and KD group were analyzed. Regarding laboratory parameters, KD individuals were found to have lower levels of L%, PA, CD4+, CD8+ and higher levels of WBC, N%, CRP, ESR, NT-proBNP, ALT, CD4+/CD8+ (P<0.05 or P<0.01). For KD group, the 53 IVIG-resistant patients had significantly higher levels of blood S100A12, HMGB1, serum IL-17A levels And N%, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT (P<0.05 or P<0.01) in comparison to the IVIG-responsive patients. For patients with acute KD in IVIG-resistant, after initial IVIG-treatment, the adjunctive therapy of IVIG, methyl prednisolone or infliximab were used, the inflammatory symptoms and laboratory inflammatory markers were improved when treated with those drugs. Conclusion: IVIG-resistant was associated with higher levels of HMGB1, S100A12, IL-17A, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT before IVIG, especially when combined, were useful predictors for IVIG-resistant in KD. In addition, the adjunctive therapy of methylprednisolone and infliximab showed more effective in relief clinical symptoms than IVIG retreatment.

scholarly journals Promising diagnostic tools for endometriosis given the pathogenic role of genetic factors

2021 ◽  
Vol 4 (1) ◽  
pp. 12-16
Author(s):  
G.A. Ikhtiyarova ◽  
◽  
M.Zh. Aslonova ◽  
Z.Sh. Kurbanova ◽  
D.M. Kalimatova ◽  
...  
Keyword(s):  
Genetic Factors ◽  
Clinical Manifestations ◽  
Hormonal Treatment ◽  
Diagnostic Tools ◽  
Pathogenic Role ◽  
Endometrial Cells ◽  
Keywords Endometriosis ◽  
Depth Study ◽  
Diagnostic Modalities

This paper discusses promising diagnostic modalities for endometriosis considering current ideas about the role of genetic factors in the pathogenesis of this condition. The hypotheses on pathogenesis and modern diagnostic approaches are addressed. The authors uncover the issues of genetic predisposition to endometriosis. The inhibition of tumor suppressor genes (CDKN2B, CDKN2 A, and ARF) and the activation of genes of early (embryonic) development of the female reproductive system (HOXA, HOXB, WNT4) lead to endometrial metaplasia. As a result, endometrial cells gain the ability to proliferate. The role of microRNA in the impaired regulation of the methylation of various genes associated with endometriosis is described. These factors specifically affect the course and clinical manifestations of endometriosis. Certain key proteins involved in the pathogenesis of endometriosis and whose synthesis is controlled by microRNA may be regarded as the diagnostic markers of endometriosis. Their in-depth study will identify novel therapeutic targets and promote the development of new non-hormonal treatment approaches to endometriosis. KEYWORDS: endometriosis, proliferation, endometrium, genes, polymorphism, diagnostic, epigenetics, microRNA. FOR CITATION: Ikhtiyarova G.A., Aslonova M.Zh., Kurbanova Z.Sh., Kalimatova D.M. Promising diagnostic tools for endometriosis given the pathogenic role of genetic factors. Russian Journal of Woman and Child Health. 2021;4(1):12–16. DOI: 10.32364/2618-8430- 2021-4-1-12-16.

scholarly journals Viruses in acute gastroenteritis in children in Pune, India

1989 ◽  
Vol 102 (2) ◽  
pp. 345-353 ◽  
Author(s):  
P. Bright Singh ◽  
M. A. Sreenivasan ◽  
Khorshed M. Pavri
Keyword(s):  
Electron Microscopy ◽  
Acute Gastroenteritis ◽  
Small Proportion ◽  
Inverse Relationship ◽  
Clinical Symptoms ◽  
Control Group ◽  
Hospitalized Children ◽  
Stool Samples ◽  
The Mean

SUMMARYA 2-year study from January 1981 to December 1982 was undertaken to determine the role of viruses in the causation of diarrhoea in hospitalized children in Pune, Maharashtra State, India. The stool samples of 426 children (213 diarrhoeal and 213 non-diarrhoeal controls) were investigated by electron microscopy and ELISA for the presence of viruses. Six morphologically distinct viruses were visualized: rotavirus, coronavirus-like particles (CVLP), adenovirus, astrovirus, calicivirus and small round virus-like particles (SRV). Rotavirus was detected in 28·6% of the diarrhoea! patients and in 1·4 % of the controls. The frequency of infection with rotavirus was highest in the children aged less than 5 years. The mean age of rotavirus-positive patients was 11 months. Although rotavirus was detected in almost every month, there has a seasonal trend for colder months when CVLP cases were fewest. However, the prevalence of CVLP was greater in the control group (23–0%) rather than in those with diarrhoea (8–9%). In the control group, CVLP were detected more frequently during the summer months. An inverse relationship between CVLP and rotavirus was observed in children. Adenovirus, astrovirus, calicivirus and SRV were detected in a small proportion of children with and without clinical symptoms of gastroenteritis.

scholarly journals FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION

2021 ◽  
Vol 23 (4) ◽  
pp. 799-804
Author(s):  
M. A. Levkovich ◽  
L. V. Kravchenko ◽  
A. A. Afonin ◽  
I. I. Krukier ◽  
A. Yu. Levkovich ◽  
...  
Keyword(s):  
Adaptive Immunity ◽  
Cytomegalovirus Infection ◽  
Clinical Symptoms ◽  
Control Group ◽  
Antiviral Immune Response ◽  
Innate And Adaptive Immunity ◽  
Group I ◽  
Severity Of The Disease

Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) – newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNγ, IFNα, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNα, IFNγ, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNα, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns. 

scholarly journals Features Of The Anamnesis Of Women With The Threat Of Miscarriage And Their Role In Determining The Risk Group

2020 ◽  
Vol 2 (09) ◽  
pp. 32-34
Author(s):  
Solieva N.K. ◽  
Negmatullaeva M.N ◽  
Sultonova N. A
Keyword(s):  
Clinical Symptoms ◽  
Risk Group ◽  
First Trimester ◽  
Control Group ◽  
Biological Factors ◽  
Lower Abdomen ◽  
Case Histories ◽  
Perinatal Center ◽  
First Trimester Of Pregnancy

Determine the role of studying socio-biological factors and obstetric-gynecological history in predicting and early diagnosis of the threat of termination in the first trimester of pregnancy. We analyzed 210 case histories of patients who were treated in the gynecological department of the Perinatal Center in Bukhara and were observed in antenatal clinics No. 6 and No. 11 of the same city for the period of 2019. All pregnant women were divided into two groups. The main I-group consisted of 110 women, whose pregnancies with the threat of termination with clinical symptoms - pain in the lower abdomen with the onset of the 1st trimester at gestation periods from 5 to 12 weeks. The control group included 100 patients with the physiological course of pregnancy II – group.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

The Use of Posterior Pericardiotomy Technique to Prevent Postoperative Pericardial Effusion in Cardiac Surgery

2012 ◽  
Vol 15 (2) ◽  
pp. 84 ◽  
Author(s):  
Canturk Cakalagaoglu ◽  
Cengiz Koksal ◽  
Ayse Baysal ◽  
Gokhan Alici ◽  
Birol Ozkan ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Heart Surgery ◽  
Artery Bypass ◽  
Pericardial Tamponade ◽  
Anxiety Level ◽  
Control Group ◽  
Echocardiographic Study ◽  
Number Of Patients ◽  
Hospital Stays

<p><b>Aim:</b> The goal was to determine the effectiveness of the posterior pericardiotomy technique in preventing the development of early and late pericardial effusions (PEs) and to determine the role of anxiety level for the detection of late pericardial tamponade (PT).</p><p><b>Materials and Methods:</b> We divided 100 patients randomly into 2 groups, the posterior pericardiotomy group (n = 50) and the control group (n = 50). All patients undergoing coronary artery bypass grafting surgery (CABG), valvular heart surgery, or combined valvular and CABG surgeries were included. The posterior pericardiotomy technique was performed in the first group of 50 patients. Evaluations completed preoperatively, postoperatively on day 1, before discharge, and on postoperative days 5 and 30 included electrocardiographic study, chest radiography, echocardiographic study, and evaluation of the patient's anxiety level. Postoperative causes of morbidity and durations of intensive care unit and hospital stays were recorded.</p><p><b>Results:</b> The 2 groups were not significantly different with respect to demographic and operative data (<i>P</i> > .05). Echocardiography evaluations revealed no significant differences between the groups preoperatively; however, before discharge the control group had a significantly higher number of patients with moderate, large, and very large PEs compared with the pericardiotomy group (<i>P</i> < .01). There were 6 cases of late PT in the control group, whereas there were none in the pericardiotomy group (<i>P</i> < .05). Before discharge and on postoperative day 15, the patients in the pericardiotomy group showed significant improvement in anxiety levels (<i>P</i> = .03 and .004, respectively). No differences in postoperative complications were observed between the 2 groups.</p><p><b>Conclusion:</b> Pericardiotomy is a simple, safe, and effective method for reducing the incidence of PE and late PT after cardiac surgery. It also has the potential to provide a better quality of life.</p>

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