Neuroimmunology of stressful pregnancy

2020 ◽  
pp. 58-62
Author(s):  
S.I. Zhuk ◽  
◽  
O.D. Shchurevska ◽  
Keyword(s):  
T Cells ◽  
High Risk ◽  
Pregnant Women ◽  
Functional Activity ◽  
Risk Groups ◽  
Internally Displaced ◽  
Pregnancy Anxiety ◽  
Pregnancy And Childbirth ◽  
Immunological Mechanisms ◽  
Complicated Pregnancy

Nowadays, almost every woman is at high risk because of stress during pregnancy, but their nature, intensity, duration of exposure are different for each woman. Extremely high-risk groups are pregnant women living in conditions of military aggression, social and humanitarian crises. The period of reactive adaptation to stress in them is rapidly changing maladaptation. This is due to neuro-immunological mechanisms, which are further implemented in the complicated pregnancy and childbirth. The objective: is to establish the stressors effects on the function of the immune system of pregnant women and their possible role in the occurrence of complications of gestation. Materials and methods. To achieve this goal, we surveyed 78 pregnant women in the third trimester of pregnancy. They were divided into 2 groups: 1 group (main) – 42 pregnant women, displaced from Lugansk and Donetsk regions, 2 group – 36 pregnant women with physiological pregnancy. General clinical studies, psychological testing (Spielberger–Hanin scale) and immunological studies: population and subpopulation composition of blood lymphocytes using the method of indirect immunofluorescence using monoclonal antibodies CD3+, CD4+, CD4+, CD8+, CD8+ CD20 + and CD16 +, study of lymphocyte functional activity and determination of CIC were conducted. Results. Total lymphopenia and significant increase in the level of circulating immune complexes (CIC) were found in pregnant women of the 1 group (forcibly displaced). This indicates a state of marked suppression of immunity (lymphopenia), complicated pregnancy, susceptibility to infectious-inflammatory processes, severe course and prolonged convalescence. The number of lymphocytes was within the normative parameters in pregnant women of the second group. Activation of the number of T cells (CD4+, CD8+, CD16+), as well as their functional activity (RBTL) were found. Which points at the activation of the T-cells immunity type. Conclusions. The results of the study prove the immunosuppressive role of psychosocial factors in internally displaced women and the presence of stress-induced decompensation of the psychoneuroendocrine system in them. Keywords: psychoneuroimmunology, pregnancy, anxiety, women–forcibly displaced, stress.

scholarly journals Peculiarities of pregnansy and childbirth in patients with a burdened gynecological anamnesis

2020 ◽  
Vol 5 ◽  
pp. 5-10
Author(s):  
T. P. Andriichuk ◽  
A. Ya. Senchuk ◽  
V. I. Chermak
Keyword(s):  
Retrospective Study ◽  
High Risk ◽  
Pregnant Women ◽  
Postpartum Period ◽  
Control Group ◽  
Careful Monitoring ◽  
Pregnancy And Childbirth ◽  
History Of ◽  
High Level ◽  
Adaptive Reactions

The objective: to study the features of pregnancy, childbirth, postpartum period, fetal status and newborns in patients with a history of chronic salpingo-оophoritis.Materials and methods. Conducted a retrospective study of 150 birth histories and neonatal development maps. All patients were divided into two groups. The main group includes 100 patients with chronic salpingo-оophoritis, for which they received anti-inflammatory treatment from 1 to 3 times before pregnancy. The control group included 50 pregnant women who did not suffer from chronic salpingo-оophoritis.Results. Our analysis of pregnancy, childbirth, fetal and neonatal status in women with chronic salpingo-оophoritis indicates that such patients have a complicated obstetric and gynecological and somatic history, which forms an unfavorable basic condition of organs and systems, imperfect adaptation to pregnancy, high risk of failure of adaptive reactions. The result is a violation of the formation and development of the mother-placenta-fetus system and, as a consequence, a high level of complications during pregnancy, childbirth and perinatal pathology.Conclusion. Patients suffering from chronic salpingo-oophoritis should be considered at high risk of possible complications during pregnancy and childbirth. This category of women needs quality preconception training and careful monitoring during pregnancy.

scholarly journals Association between clonogenic cell growth and clinical risk group in B- cell chronic lymphocytic leukemia

Blood ◽  
1990 ◽  
Vol 76 (1) ◽  
pp. 142-149 ◽  
Author(s):  
R Dadmarz ◽  
SN Rabinowe ◽  
SA Cannistra ◽  
JW Andersen ◽  
AS Freedman ◽  
...  
Keyword(s):  
T Cells ◽  
High Risk ◽  
Cell Growth ◽  
Chronic Lymphocytic Leukemia ◽  
B Cell ◽  
Risk Groups ◽  
Lymphocytic Leukemia ◽  
Cloning Efficiency ◽  
Activated T Cells ◽  
Clonogenic Cells

Abstract Chronic lymphocytic leukemia of B-cell origin (B-CLL) is a disease with a variable clinical course, despite the fact that the neoplastic cells in this disorder are homogeneous with respect to morphology, immunophenotype, and cell cycle stage. To further investigate the heterogeneity observed in the clinical behavior of B-CLL, we determined the phenotype and growth requirements of clonogenic cells from 28 patients with B-CLL from low-, intermediate-, and high-risk groups as defined by the Rai staging system. Using methyl-cellulose as a semi- solid media with feeder cells and/or growth factors, colonies were observed with one or more of the culture conditions tested in 25 of 28 CLLs. Phenotypic analysis of colonies demonstrated that the clonogenic cells uniformly expressed la, CD19, CD20, CD5, and the identical light chain as the original CLL cell cultured. However, heterogeneity was observed in clonogenic B-CLL cell growth among the three different CLL risk groups. Clonogenic cells from patients with low-risk CLL required either irradiated unstimulated T cells, with or without conditioned media (CM) or irradiated activated T cells alone for colony formation. Both the number of colonies (227 +/- 15) as well as the number of cells per colony (220 +/- 82) were large, with a mean cloning efficiency of 0.39%. In contrast, clonogenic cells from patients with intermediate- and high-risk CLL required the combination of both irradiated activated T cells and CM. As compared with the low-risk CLLs, both the number and size of the colonies formed by the intermediate- (74 +/- 17, 70 +/- 39) and high- (83 +/- 28, 40 +/- 14) risk groups were significantly lower (P less than .0001). Similarly, the mean cloning efficiency was significantly reduced to 0.15% and 0.14%, respectively. None of the recombinant cytokines (interleukin 1 [IL-1] to IL-7, tumor necrosis factor, alpha and gamma-interferon, B-cell growth factor, and granulocyte macrophage colony-stimulating factor) alone or in combination with each other could entirely replace the stimulatory effect of the activated T cells. These data suggest that clinical progression of B-CLL is associated with a loss of clonogenic potential in the circulating pool of neoplastic cells, which require as yet undefined factors provided by activated T cells and CM.

scholarly journals Comparação da Aplicação da EER Hema-Obs a 250 Gestações da Consulta de Hematologia-Obstetrícia do Centro Hospitalar São João, Portugal Versus EER Galit Sarig a 90 Gestações no Rambam Health Care Campus, Israel

2015 ◽  
Vol 28 (2) ◽  
pp. 189
Author(s):  
Ana Salselas ◽  
Inês Pestana ◽  
Francisco Bischoff ◽  
Mariana Guimarães ◽  
Joaquim Aguiar Andrade
Keyword(s):  
High Risk ◽  
Risk Stratification ◽  
Pregnant Women ◽  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Risk Groups ◽  
Low Risk ◽  
Very High ◽  
Receiver Operating

<strong>Introduction:</strong> Pregnant women with thromboembolic diseases, previous thrombotic episodes or thrombophilia family history were supervised in a multidisciplinary Obstetrics/ Hematology consultation in Centro Hospitalar São João EPE, Porto, Portugal. For the evaluation and medication of these women, a risk stratification scale was used.<br /><strong>Purposes:</strong> The aim of this study was to validate a Risk Stratification Scale and thromboprophylaxis protocol by means of comparing it with a similar scale, developed and published by Sarig.<br /><strong>Material and Methods:</strong> We have compared: The distribution, by risk groups, obtained through the application of the two scales on pregnant women followed at Centro Hospitalar São João, Porto, Portugal, consultation; the sensibility and specificity for each one of the scales (DeLong scale, applied to Receiver Operating Characteristic) curves; the outcomes in pregnancies followed in Hospital São João, Porto, Portugal<br /><strong>Results:</strong> According to our Hema-Obs risk stratification scale, 29% were allocated to low-risk, 47% to high-risk and 24% to very-high-risk groups. According to Galit Sarig risk stratification scale, 24% were considered low-risk, 53% moderate, 16% high-risk and 7% as very high-risk group. In our study we observed 9% of spontaneous abortions, in comparison with 18% in the Galit Sarig cohort. From the application of Receiver Operating Characteristic curve to both risk stratification scales, the results of the calculated areas were 58,8% to our Hema-Obs risk stratification scale and 38,7% to Galit Sarig risk stratification scale, with a Delong test significancie of p = 0.0006.<br /><strong>Conclusions:</strong> We concluded that Hema-Obs risk stratification scale is an effective support for clinical monitoring of therapeutic strategies.

scholarly journals Kontribusi Jumlah Kehamilan (Gravida) Terhadap Komplikasi Selama Kehamilan dan Persalinan

2019 ◽  
Vol 2 (1) ◽  
pp. 9
Author(s):  
Sulastri Sulastri ◽  
Arina Maliya ◽  
Ni'mah Mufidah ◽  
Eka Nurhayati
Keyword(s):  
Risk Factors ◽  
High Risk ◽  
Data Collection ◽  
Pregnant Women ◽  
Health Information ◽  
Pregnancy And Childbirth ◽  
High Risk Factors ◽  
Work Area ◽  
Life Threatening ◽  
Quantitative Descriptive

                             Introduction. Obstetric and neonatal emergencies are a condition that can be life threatening and can occur both during pregnancy and childbirth. One of the causes of emergency is the complication of pregnancy, where this complication can arise due to risk factors during pregnancy, one of which is the gravida. Pregnant women with primigravida and gravida = 4 are high-risk factors for pregnancy and childbirth. The purpose of this study was to determine the contribution of the number of pregnancies (gravida) to mothers who had complications of pregnancy and childbirth in the work area of the Gatak Health Center, Sukoharjo. Methods. This research is a quantitative descriptive with a retrospective approach, data collection using the record or other documents in the form of health information for pregnant women with pregnancy comorbidities taken based on data from January 1 to December 31, 2018, in total sampling. Result. Of the 224 data of pregnant women with comorbidities, among them experienced pre-eclampsia (67.1%). The characteristics of respondents were based on the most age at 20-35 years old (82.5%), multigravida status (84.8%), and SC delivery (67%). Discussion. The importance of regulating and monitoring the number of pregnancies (gravida) of mothers to prevent complications of pregnancy and childbirth and to improve the degree of life of a mother.

scholarly journals Metabolic syndrome related gene signature predicts the prognosis of patients with pancreatic ductal carcinoma. A novel link between metabolic dysregulation and pancreatic ductal carcinoma

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Weiyang Cai ◽  
Wenming Bao ◽  
Shengwei Chen ◽  
Yan Yang ◽  
Yanyan Li
Keyword(s):  
T Cells ◽  
High Risk ◽  
Cox Regression ◽  
Ductal Carcinoma ◽  
Risk Groups ◽  
Cancer Genome ◽  
Low Risk ◽  
Pancreatic Ductal Carcinoma ◽  
Blue Module ◽  
Geo Database

Abstract Background Pancreatic cancer is one of the most common malignancies worldwide. In recent years, specific metabolic activities, which involves the development of tumor, caused wide public concern. In this study, we wish to explore the correlation between metabolism and progression of tumor. Methods A retrospective analysis including 95 patients with pancreatic ductal adenocarcinoma (PDAC) and PDAC patients from The Cancer Genome Atlas (TCGA), the International Cancer Genome Consortium (ICGC), and The Gene Expression Omnibus (GEO) database were involved in our study. Multivariate Cox regression analysis was used to construct the prognosis model. The potential connection between metabolism and immunity of PDAC was investigated through a weighted gene co-expression network analysis (WGCNA). 22 types of Tumor-infiltrating immune cells (TIICs) between high-risk and low-risk groups were estimated through CIBERSORT. Moreover, the potential immune-related signaling pathways between high-risk and low-risk groups were explored through the gene set enrichment analysis (GSEA). The role of key gene GMPS in developing pancreatic tumor was further investigated through CCK-8, colony-information, and Transwell. Results The prognostic value of the MetS factors was analyzed using the Cox regression model, and a clinical MetS-based nomogram was established. Then, we established a metabolism-related signature to predict the prognosis of PDAC patients based on the TCGA databases and was validated in the ICGC database and the GEO database to find the distinct molecular mechanism of MetS genes in PDAC. The result of WGCNA showed that the blue module was associated with risk score, and genes in the blue module were found to be enriched in the immune-related signaling pathway. Furthermore, the result of CIBERSORT demonstrated that proportions of T cells CD8, T cells Regulatory, Tregs NK cells Activated, Dendritic cells Activated, and Mast cells Resting were different between high-risk and low-risk groups. These differences are potential causes of different prognoses of PDAC patients. GSEA and the protein–protein interaction network (PPI) further revealed that our metabolism-related signature was significantly enriched in immune‐related biological processes. Moreover, knockdown of GMPS in PDAC cells suppressed proliferation, migration, and invasion of tumor cells, whereas overexpression of GMPS performed oppositely. Conclusion The results shine light on fundamental mechanisms of metabolic genes on PDAC and establish a reliable and referable signature to evaluate the prognosis of PDAC. GMPS was identified as a potential candidate oncogene with in PDAC, which can be a novel biomarker and therapeutic target for PDAC treatment.

scholarly journals A Seroprevalence Study for HBV in Pregnant Women in Greece: High Risk Migrant Groups and Opportunities for Intervention

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Anagnostatou N ◽  
◽  
Hatzidaki E ◽  
Galanakis E ◽  
◽  
...  
Keyword(s):  
High Risk ◽  
Pregnant Women ◽  
Health Planning ◽  
Action Plan ◽  
Perinatal Care ◽  
Risk Groups ◽  
Quality Health Care ◽  
Roma Population ◽  
Seroprevalence Data ◽  
Migrant Groups

Background: Perinatal transmission of HBV leads to chronic infection in up to 90% of neonates. Focused prenatal screening and appropriate treatment of pregnant women and neonates is necessary for the elimination of hepatitis B, as was stated in the 2017 WHO Action Plan for HBV. Information on seroprevalence of HBV in Greece, especially in pregnant women, is scarce and rather outdated. Seroprevalence data specifically for high-risk groups, such as immigrants, is necessary for proper public health planning and elimination of vertical transmission and this study will struggle to fill the gap that exists in Greece. Methods: HBsAg status of pregnant women delivering during 2017 in Crete was studied. Seroprevalence was estimated for the whole population and each ethnic group separately. Results: The mean age of the participants was 30.38 (±6) years. Their origin was Greek (76.76 %), Albanian (10.18%), Bulgarian (3.79%), Roma population (2.44%), Russia and Former Republics of Russia (2.06%), Romanian (1.95%), Central Europe (0.70%) refugees from Syria, Morocco, Egypt (0.55%), and East Asian (0.43%). The HBsAg (+) seroprevalence was 1.5%. The seroprevalence of Greeks was 0.5%, while Albanians, Bulgarians, Romanians and Roma had 4.3%, 5.7%, 2.8%, and 11.1% respectively (p<0.001). Conclusions: Defining specific at-risk groups in each country is fundamental, since MTCT is the principal mode of transmission in high prevalence settings. Our study revealed high seroprevalence in certain migrant groups and Roma women. This information is essential for proper planning of perinatal care in Greece, especially taking into account that these underprivileged groups often lack quality health care.

Prevalence of infection by HTLV-I/II among pregnant women and high-risk groups in the Peloponnese peninsula, Greece

2006 ◽  
Vol 17 (8) ◽  
pp. 543-546 ◽  
Author(s):  
Parthenopi M Tseliou ◽  
Nicholas Spanakis ◽  
Anna Spiliotakara ◽  
Antonios Markogiannakis ◽  
Nicholas J Legakis ◽  
...  
Keyword(s):  
High Risk ◽  
Pregnant Women ◽  
Risk Groups

scholarly journals Prognostic Value of Immune-Related genes in the Tumor Microenvironment of Glioma - Analyzed by Integrated Bioinformatics

2021 ◽  
Author(s):  
Yali Zhong ◽  
Xiaobin Luo ◽  
Fubing Yang ◽  
Xinling Song
Keyword(s):  
T Cells ◽  
High Risk ◽  
Risk Prediction ◽  
Immune Cell ◽  
Risk Group ◽  
Risk Groups ◽  
High Risk Group ◽  
Low Risk ◽  
Lasso Regression ◽  
Immune Related Genes

Abstract Object: Immune related genes play an important role in the process of tumor genesis and development. Therefore, we aim to find the Immune genes which are related to the prognosis of glioma patients, and to explore the infiltration of Immune cells in glioma microenvironment. Methods We downloaded the data of the glioma samples from the CGGA database, and performed batch correction to screen the primary glioma samples for subsequent analysis. Then the ESTIMATE algorithm was used to deal with the Stromal scores and Immune scores of the primary glioma samples, and the difference was analyzed. Then the common Immune related genes (IRGs) were obtained by intersecting with the Immune genes in the ImmPort database. Moreover, we used common IRGs to construct protein-protein interaction (PPI) networks, from which we screened the top 30 genes with high connectivity, and Lasso regression was used to screen the IRGs. Lastly, we obtained the combined genes, which were overlapped both in the top 30 high-connection genes and Lasso regression genes. The final genes were used to construct COX risk prediction models. The accuracy of the model were verified by the TCGA glioma data, and the model genes were analyzed for Immune-related pathways, as well as the Hallmark and KEGG enrichment. Additionally, we used CIBERSOFT algorithm to estimate the Immune cell content of the samples, and analyzed the differences, correlations and survival of the Immune cells in high and low risk groups. Results Firstly, a total of 117 IRGs were obtained from the gene sets, which were overlapped in the data of Stromal score, Immune score and ImmPort database. Secondly, the top 30 genes were selected after the PPI network, and another 26 genes were screened out after the Lasso regression algorithm. And then, six coexist IRGs were obtained from the intersecting sets. Furthermore, the COX risk prediction model was constructed and tested, showing that the overall survival rate of the high-risk group was about 50% of that of the low-risk group. We observed that the high-risk group were enriched in Immune response and Immune process. Most importantly, in KEGG pathways, the high-risk groups were mainly enriched in p53 signaling pathway, JAK-STAT signaling pathway, pathways in cancer and cell cycle. By estimating the Immune cell contents, we also found that the Immune cell Plasma cells, T cells CD8, T cells CD4 naïve, T cells regulatory (Tregs), Macrophages M0 and Neutrophils were higher in high-risk groups, when compared to the low-risk group, with significant difference. Finally, the correlation analysis showed that the degree of Immune infiltration in high-risk groups was related to T cells regulatory (Tregs), Macrophages M0 and Neutrophils. Conclusion A COX risk prediction model of 6 genes was successfully constructed, which was enriched in Immune-related pathways. Meanwhile, survival analysis and TCGA data validation revealed significant differences in the model genes in the overall survival of the glioma patients, and the degree of Immune infiltration in the model was associated with T cells regulatory (Tregs), Macrophages M0 and Neutrophils.

scholarly journals ACCOMPANY OF PREGNANT WOMEN HIGH RISK THROUGH PREGNANT CLASS IN KARANGMANGU VILLAGE PURWOJATI DISTRCT BANYUMAS REGENCY

2019 ◽  
Vol 5 (2) ◽  
pp. 28-38
Author(s):  
Surtinigsih ◽  
Linda yanti
Keyword(s):  
Back Pain ◽  
High Risk ◽  
Early Detection ◽  
Pregnant Women ◽  
Community Health Center ◽  
Energy Deficiency ◽  
Pregnancy And Childbirth ◽  
Chronic Energy Deficiency ◽  
And Child Health ◽  
The Coordinator

Pregnancy and childbirth are physiological but require good preparation and assistance from family and health professionals to minimize the risk of pregnancy and childbirth complications.  From the results of interviews with the Coordinator of Maternal and Child Health at the Purwojati Community Health Center, there were quite a number of problems, including underage or over 35 years of pregnancy, pregnancy outside marriage, pregnancy with Chronic Energy Deficiency (KEK) and anemia in pregnancy. Discomfort of many trimester III pregnancies are also found such as back pain, constipation, frequent urination and swelling in the legs. The purpose of this activity is the formation and activation of classes of pregnant women who are equipped with complementary care and synergize between standard care with innovations that are expected to maximize services at both the Posyandu or Puskesmas levels. Assistance of high risk mothers is carried out with early detection, physical examination, laboratory examination, health education, and also some activities such as pelvic rocking exercise. From the results of early detection conducted in Karangmangu Village, there were cases of pregnant women experiencing chronic energy deficiency of 16.2%, Anemia of 21.1%, pregnancy with diabetes risk of diabetes 8.1%, positive urine protein 5.4% and others pregnancy is accompanied by an infectious disease of 5.4%.  In addition to the findings of the problem there are also complaints that are felt by many pregnant women, including constipation, cramps, frequent urination and back pain. The conclusion of this activity is the formation of a class of pregnant women in Karangmangu Posyandu 2 which is implemented in Karangmangu Village Polyclinic so that the pregnant class is not focused in Purwojati Puskesmas, the detection of pregnancy problems so that assistance can be made especially to high risk pregnant women, the increase in knowledge of mothers pregnancy and back pain, cramps, constipation can be minimized with pelvic rocking exercises

scholarly journals COMPARATIVE ASPECTS OF PLACENTAL DYSFUNCTION IN WOMEN USING DIFFERENT METHODS OF CONTRACEPTION IN ANAMNESIS

2020 ◽  
Vol 5 ◽  
pp. 34-40
Author(s):  
Oleksandra Lubkovska
Keyword(s):  
Pregnant Women ◽  
Clinical Laboratory ◽  
Risk Groups ◽  
Oral Contraception ◽  
Pregnancy And Childbirth ◽  
Diagnostic Measures ◽  
Perinatal Pathology ◽  
Satisfactory Condition ◽  
Fetoplacental Complex ◽  
History Of

The aim. To reduce the incidence of perinatal pathology in women who have used various methods of contraception, based on the study of the functional state of the fetoplacental complex (FPS), as well as improving diagnostic measures and tactics of pregnancy. Materials and methods. 140 pregnant women were studied with their division into control and 3 test groups depending on the method of contraception in the anamnesis, using clinical, laboratory and instrumental research methods during pregnancy and childbirth. Results. The study found that women with a history of intrauterine contraception (IUC), in contrast to combined oral contraception (COC) and combined oral contraception containing folate (COC+F), had a higher incidence of complications during pregnancy and childbirth, as well as fewer newborns with a satisfactory condition at birth. Assessment of folic acid levels at 6-8 weeks of gestation showed significantly better results among women with a history of COC+F, compared with IUC and COC. In the group of women with IUC in the anamnesis, significantly worse mean endocrinological values prevailed, and there was also a greater number of pregnant women with disorders of fetal-placental blood flow. More pronounced dystrophic changes in the placentas of women in this group were pathomorphologically confirmed. Conclusions. The presence in the anamnesis of IUC is accompanied by a high proportion of pregnant women with various pathological conditions and is a risk factor for FPS dysfunction. The use of COC+F in the anamnesis is the best option for women of different risk groups.

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