scholarly journals Saponins accumulation and antimicrobial activities on shallot (Allium cepa L.) from marginal land

Jurnal Agro ◽  
2022 ◽  
Vol 8 (2) ◽  
pp. 188-198
Author(s):  
Nur Aeni Ariyanti ◽  
Sonia Latifa
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Fusarium Oxysporum ◽  
Allium Cepa ◽  
Ralstonia Solanacearum ◽  
Agricultural Land ◽  
Antimicrobial Activities ◽  
Observational Research ◽  
Sandy Land ◽  
Allium Cepa L

Saponins are one of the secondary metabolites found in Shallot (Allium cepa L.), particularly in the roots. Microclimate differences in the cultivation area are thought to have a significant impact on the production of secondary metabolites, such as saponins. This research aimed to observe the saponins content in the root of shallot plants cultivated in marginal agricultural land and their antimicrobial activity against bacteria (Ralstonia solanacearum) and fungus (Fusarium oxysporum). This research was observational research with a random sampling method. The samples were collected from the shallot plantation with two different cultivation conditions. Two varieties of ‘Bima' and 'Tiron' cultivated by farmers in sandy coastal land Samas, Bantul were used. The plants were harvested at 1, 1.5, and 2 months after planting, respectively. The crude saponins extract was used to test antimicrobial activity. Shallot plants cultivated in marginal coastal sandy land produced higher saponins accumulated in their roots. The saponins production increased along with the maturity of shallot plants, both cultivated in marginal coastal sandy land and regular paddy field. The saponins extracted from the roots of shallots cultivated in both marginal and regular land showed higher antimicrobial activity than antifungal activity. Saponin merupakan salah satu metabolit sekunder yang terdapat pada bawang merah (Allium cepa L.), terutama pada bagian akar. Perbedaan iklim mikro pada lahan budidaya diduga akan berpengaruh terhadap produksi metabolit sekunder termasuk saponin. Tujuan penelitian ini adalah untuk mengetahui kandungan saponin pada bagian akar tanaman bawang merah yang dibudidayakan di lahan pertanian marginal serta aktivitas antimikrobanya terhadap bakteri (Ralstonia solanacearum) dan jamur (Fusarium oxysporum). Penelitian ini merupakan penelitian observasi dengan metode pengambilan sampel secara acak. Sampel berasal dari perkebunan bawang merah dengan dua lahan budidaya yang berbeda. Digunakan dua varietas yaitu 'Bima' dan 'Tiron' yang dibudidayakan oleh petani di daerah pantai Samas,kabupaten Bantul. Bahan tanaman dipanen pada tiga waktu berbeda, yaitu 1 bulan, 1,5 bulan dan 2 bulan berturut-turut setelah tanam. Ekstrak kasar saponin digunakan untuk menguji aktivitas antimikrobanya. Tanaman bawang merah yang dibudidayakan di lahan marginal pasir pantai menghasilkan saponin yang  lebih tinggi yang terakumulasi pada akarnya. Produksi saponin semakin meningkat seiring dengan umur tanaman bawang merah, baik yang dibudidayakan di lahan marginal pasir pantai maupun di lahan sawah biasa. Saponin yang diekstraksi dari akar bawang merah yang dibudidayakan di lahan marginal dan lahan biasa menunjukkan aktivitas antimikroba yang lebih tinggi daripada aktivitas antijamurnya.

Biochemical Estimation and Antimicrobial Activities of the Extracts of Caesalpinia Sappan Linn.

1970 ◽  
Vol 46 (4) ◽  
pp. 429-436
Author(s):  
N Senthilkumar ◽  
S Murugesan ◽  
N Banu ◽  
S Supriya ◽  
C Rajeshkannan
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Antimicrobial Activities ◽  
Oriental Medicine ◽  
Good Source ◽  
Caesalpinia Sappan ◽  
Indian Medicinal Plant ◽  
Biochemical Estimation ◽  
Pathogenic Microbes ◽  
Ethyl Acetate Extracts

Caesalpinia sappan Linn., a traditional Indian medicinal plant used widely in oriental medicine. The plant extracts were found to be a good source of secondary metabolites, vitamins and metals. The extracts were further tested against certain human pathogenic microbes. The methanol and ethyl acetate extracts of the heartwood was found to be effective against certain pathogenic microbes. Key words: Caesalpinia sappan; Secondary metabolites; Vitamins; Antimicrobial activity DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9586 BJSIR 2011; 46(4): 429-436

scholarly journals Antimicrobial Activity of Securamines From the Bryozoan Securiflustra securifrons

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199618
Author(s):  
Kine Ø. Hansen ◽  
Ida K. Ø. Hansen ◽  
Céline S. Richard ◽  
Marte Jenssen ◽  
Jeanette H. Andersen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Streptococcus Agalactiae ◽  
Mode Of Action ◽  
Treatment Options ◽  
Antimicrobial Activities ◽  
The Arctic ◽  
Pathogenic Microorganisms ◽  
Human Pathogenic Bacterium

Natural products and their derivatives have served as powerful therapeutics against pathogenic microorganisms and are the mainstay of our currently available treatment options to combat infections. As part of our ongoing search for antimicrobial natural products from marine organisms, one fraction prepared from the Arctic marine bryozoan Securiflustra securifrons was found to be active against the human pathogenic bacterium Streptococcus agalactiae (gr. B). Chemical investigation of the fraction revealed that it contained several variants of the highly modified secondary metabolites known as securamines. The securamines are alkaloids sharing a common isoprene-histamine-tryptamine backbone. In this study, we describe the antimicrobial activities of securamine C, E, and H – J (4, 5, and 1-3) and the attempt to deconvolute the mode of action of 1.

scholarly journals Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9342
Author(s):  
Chao An ◽  
Saijian Ma ◽  
Xinwei Shi ◽  
Wenjiao Xue ◽  
Chen Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
High Performance ◽  
Fungal Endophytes ◽  
Folk Medicine ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Qinling Mountains

Endophytic fungi have been emerged as fruitful resources for producing structurally fascinating and biologically active secondary metabolites. However, endophytic fungi from medicinal plants of Qinling Mountains–the most important natural climatic boundary between the subtropical and warm temperate zones of China with an astonishingly high level of biodiversity–have rarely been explored as potential sources of novel fungal species and active secondary metabolites. In this study, a total of 371 fungal colonies were successfully isolated from 510 tissue segments of the medicinal Tupistra chinensis Baker collected from Qinling Mountains, China. Roots of T. chinensis Baker are used as a folk medicine to ameliorate pharyngitis and treat rheumatic diseases. A total of 100 representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), seven classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Microbotryomycetes, Agaricomycetes, Leotiomycetes, Mortierellomycetes), and at least 35 genera. The genera of Collectotrichum (IF, 29.92%), Fusarium (IF, 8.36%), Aspergillus (IF, 8.09%), and Dactylonectria (IF, 5.39%) were most frequently isolated from the tissues of T. chinensis Baker. The Species Richness Index (S, 65) and the Shannon-Wiener Index (H′, 3.7914) indicated that T. chinensis Baker harbored abundant fungal resources. Moreover, five isolates were potential new taxa because of low similarity of ITS sequences ranged from 95.09%∼96.61%. Fifteen out of 100 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Two important lead compounds produced by two stains (F8047 and F8075) with high antimicrobial activities were identified using high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC–QTOF MS) analyses. In addition, it was noteworthy that the strain F8001, which may be a potential new species, showed antimicrobial activity and should be investigated further. Overall, these results indicated that the endophytic fungi from T. chinensis Baker could be exploited as a novel source of bioactive compounds.

scholarly journals Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling

Plant Science ◽  
2016 ◽  
Vol 246 ◽  
pp. 128-138 ◽  
Author(s):  
Mostafa Abdelrahman ◽  
Fatma Abdel-Motaal ◽  
Magdi El-Sayed ◽  
Sudisha Jogaiah ◽  
Masayoshi Shigyo ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Allium Cepa ◽  
Metabolite Profiling ◽  
Induced Defense ◽  
Trichoderma Longibrachiatum ◽  
Allium Cepa L

scholarly journals Antimicrobial activity and thiosulfinates profile of a formulation based on Allium cepa L. extract

2017 ◽  
Vol 15 (1) ◽  
pp. 175-181
Author(s):  
O. Orăşan ◽  
R. Oprean ◽  
A. Saplonţai-Pop ◽  
M. Filip ◽  
R. Carpa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Allium Cepa ◽  
High Performance ◽  
Ultrapure Water ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Liquid Extract ◽  
Allium Cepa L

AbstractBackgroundAllium species extracts including Allium cepa L. contain sulfur compounds, known for their antiplatelet, antimicrobial, antineoplasic activities.MethodologyAntibacterial activity of a formulation based on A. cepa extracts-liquid and lyophilized samples, has been demonstrated using two classes of bacteria: Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) and three methods: discs soaked with liquid extract, the wells method in the culture medium, filled with the liquid extract and lyophilized formulation extracts transformed by the incorporation of ultrapure water. The second part of this study includes identification of thiosulfinates compounds from the studied samples by high performance liquid chromatography - mass spectrometry (HPLC-MS).ResultsThe most important inhibition and the highest antibacterial effficiency were observed against Gram-positive bacteria, such as Staphylococcus aureus. The HPLC-MS thiosulfinates profile of the tested formulation extracts shows the presence of seven thiosulfinate compounds, MeS(O)S 1-propenyl (E,Z); n-PrS(O)S 1-propenyl-(E); n-PrS(O)S 1-propenyl-(Z); trans-zwiebelane; n-PrS(O)CHEtSS-1propenyl; 1-propenylS(O)CHEtSS1-propenyl, which may be responsible for antibacterial activity.ConclusionTesting antimicrobial effects using the three mentioned methods confirmed the antimicrobial activity of the tested samples based on A. cepa extracts, with a demonstrated content of seven thiosulfinate compounds.

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