PENGARUH PENAMBAHAN EKSTRAK JAGUNG MANIS  DAN AIR KELAPA PADA MEDIA MS SECARA IN VITRO TERHADAP  INDUKSI TUNAS ADVENTIF BAWANG PUTIH (Allium sativum L.)

[SWEET CORN EXTRACT AND COCONUT WATER EFFECT TO INDUCE GARLIC (Allium sativum L.) ADVENTIST SHOOTS BY IN-VITRO CULTURE]. Garlic (Allium sativum L.) is one of the horticultural plants used daily for various uses. This study aims to determine the best extract concentration of sweet corn and coconut water and its interaction for induction garlic Adventist shoots (Allium sativum L.) var. Lumbu Hijau. The study was conducted at the Biotechnology Laboratory, Agriculture Faculty, UPN ”Veteran” East Java, in February – April 2021. The experimental design used was a factorial completely randomized design (CRD). The first factor is four levels of extract of sweet corn concentration (0, 50, 100, and 150 g/L), while the second factor is four levels of coconut water concentration (0, 50, 100, and 150 mL/L). The results showed that the extract of sweet corn concentration-time treatment increased the growing adventist shoots percentage, adventist shoots number, and adventist shoots size. The section of sweet corn concentration of 150 g/L increased the rate of growing adventist shoots, adventist shoots number, and adventist shoots size. The treatment of coconut water concentration and its interaction with the extract of sweet corn concentration is still not able for induction garlic adventist shoots (Allium sativum L.) of the var. Lumbu Hijau.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Application of Young Coconut Water and “Kascing” Fertilizer to Red Palm Seed Germination

Jurnal ILMU DASAR ◽

10.19184/jid.v18i1.2115 ◽

2017 ◽

Vol 18 (1) ◽

pp. 17

Author(s):

Fatimatuz Zuhro ◽

Hasni Ummul Hasanah ◽

Sukadi Sukadi

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Water Concentration ◽

Germination Percentage ◽

Coconut Water ◽

Randomized Design ◽

Completely Randomized Design

Redpalm isornamentplantwhichpotentiallytobecultured.Thescarcityofredpalmmadeithashighmarket value and need to be preserved. However, the germination growth of red palm is slower than other kinds of palm, so the best way to germinate the red palm seed is needed. The aim of this research is to determine the effect of apliccation some young coconut water concentration and kascing fertilizer to red palm seed germination. The research used factorial completely randomized design with 5 replication, the first factor is young coconut water concentration (0%, 25%, 50%, 75%, and 100%) and the second factor is germination media (steril sand and kascing fertilizer). Variables measured were live germination percentage, speed germination rate, radicula length, and plumula height. Results of this study showed that the best germination media is steril sand and 0% concentration of young coconut water.

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Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201967010009 ◽

2019 ◽

Vol 67 (1) ◽

pp. 9-18

Author(s):

Enik Akhiriana ◽

Samanhudi ◽

Ahmad Yunus

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Tribulus Terrestris ◽

Emergence Time ◽

Shoot Height ◽

Randomized Design ◽

Number Of Leaves ◽

Indole 3 Acetic Acid ◽

Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

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PEMANFAATAN AIR KELAPA UNTUK MENINGKATKAN PERTUMBUHAN AKAR STEK TUNAS AKSILAR Andrographis paniculata Nees

EKSAKTA Berkala Ilmiah Bidang MIPA ◽

10.24036/eksakta/vol18-iss02/54 ◽

2017 ◽

Vol 18 (02) ◽

pp. 62-68

Author(s):

Retno Prihatini

Keyword(s):

Root Length ◽

Water Concentration ◽

Height Growth ◽

Andrographis Paniculata ◽

Coconut Water ◽

Axillary Buds ◽

Root Number ◽

Randomized Design ◽

Completely Randomized Design ◽

Cutting Height

The research has been conducted to find out the best concentration of coconut water in the induction of roots of cuttings axillary buds Andrographis paniculata Nees. This research was conducted in Plant Physilogy of Division, Department of Biology, Faculty of Math and Natural Science, AndalasUniversity, for 12 weeks. The research used Completely Randomized Design. The material used was axillary buds A. paniculata with nodal number 3. treated for 30 minutes in coconut water 10, 15 and 20% and aquadest as a controle each with 6 replications. Observations were done on live percentage, root length, root numerous and cuttings height after 12 weeks planting. The results showed that the concentration 10 to 15% of coconut water was able with significant to induce the root growth of cutting axillary buds A. Paniculat. The concentration 15% of coconut water was the best in inducing cutting height growth, each with an average root length is 8.2 cm and the average number of roots that formed is 16, 0 and the average cutting height is 22.7 cm. There was an increase of average of cuttings height growth by 86% which was supported by the average increase of root length and root number of 105.0% and 280.9% on the occasion of cuttings of axillary buds in coconut water concentration 15%.

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Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽

10.15575/1344 ◽

2017 ◽

Vol 4 (2) ◽

pp. 97-109

Author(s):

Lamro Purba ◽

Erni Suminar ◽

Denny Sobardini ◽

Wieny Rizky ◽

Syariful Mubarok

Keyword(s):

Tissue Culture ◽

Leaf Number ◽

Shoot Induction ◽

Seed Technology ◽

Randomized Design ◽

Rooting Media ◽

Completely Randomized Design ◽

Four Levels ◽

Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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Pengaruh lama perendaman menggunakan air kelapa terhadap maskulisasi ikan lele masamo (Clarias sp)

Acta Aquatica Aquatic Sciences Journal ◽

10.29103/aa.v6i2.1398 ◽

2019 ◽

Vol 6 (2) ◽

pp. 58

Author(s):

Suardi Laheng ◽

Anjar Widyastuti

Keyword(s):

Treatment Efficacy ◽

Immersion Time ◽

Coconut Water ◽

Randomized Design ◽

Completely Randomized Design ◽

Four Levels

AbstrakTujuan penelitian untuk mengetahui bagaimana proses maskulinisasi pada ikan lele masamo dan bagaimana hasil maskulinisasi ikan lele masamo menggunakan air kelapa. Penelitian ini dilaksanakan di Balai Benih Ikan Lokal Tatatanga, Kota Palu. Hewan uji yang digunakan dalam penelitian ini yaitu larva ikan lele masamo (Clarias sp) berjumlah 360 ekor berumur 7 hari. Rancangan penelitian yang digunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga kali ulangan, yaitu : P1 (lama waktu perendaman 5 jam), P2 (lama waktu perendaman 10 jam), P3 (lama waktu perendaman 15 jam) dan P4 (lama perendaman 20 jam). Larva ikan Lele masamo berumur 7 hari direndam dalam air kelapa dengan dosis 30%/L. Pengamatan dilakukan pada umur ikan 40 hari. Hasil penelitian menunjukkan rata-rata jumlah persentase ikan lele jantan pada perlakuan P1; P2; P3 dan P4 masing-masing adalah 53,89; 65,56; 44,44 dan 0,00%. Perlakuan P2 efektif dalam maskulinisasi ikan lele masamo melalui perendaman air kelapa.Kata kunci: maskulinisasi; lele masamo; air kelapa; pertumbuhanAbstractThis present work aimed to investigate the masculinization of masamo catfish (Clarias sp) larvae by immersion with coconut water. The experiment was carried out in local fish nurtury centre in Tatatanga, Palu. The 7-days-old larvae (360 individuals) of masamo catfish was used. A completely randomized design was arranged, using four levels of immersion time: 5, 10, 15, and 20 (named as P1, P2, P3, and P4, respectively). The immersion was carried out in coconut water and made three triplicates. After the fish reached 40 days old, the treatment efficacy was evaluated. The results showed that the percentage of male catfish differed sharply, i.e. 53,89% (P1), 65,56% (P2), 44,44% (P3), and 0,00% (P4). To sum up, we concluded that P2 demonstrated the highest percentage, being the most effective masculinization treatment on masamo catfish.Keywords: masculinization; masamo catfish; coconut water; growth

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In Vitro Response of Protocorm Grammatophyllum stapeliiflorum (Teijsm. & Binn.) J.J.Sm. Orchid in Growth on Several Media Composition

JURNAL BIOLOGI UNAND ◽

10.25077/jbioua.7.2.118-125.2019 ◽

2019 ◽

Vol 7 (2) ◽

pp. 118

Author(s):

Ranny Wirmasari ◽

Mayta Novaliza Isda

Keyword(s):

Growth And Development ◽

Coconut Water ◽

Media Composition ◽

Randomized Design ◽

In Vitro Response ◽

Completely Randomized Design ◽

Original Habitat ◽

Test Treatment ◽

Number Of Shoots

Family Orchidaceae has about 800 genera which are already difficult to find and almost extinct, including the orchid genus Grammatophyllum. A species of rare orchids, Grammatophyllum stapeliiflorum, is hard to find in original habitat. The addition of Growmore, BAP and 15% coconut water is expected to increase the growth and development of the G. stapeliiflorum orchid protocorms. This study use a Completely Randomized Design, consisting of 6 treatments (control, 1 and 3 mg/l BAP, 1 and 3 g/l Growmore, and 150 ml/l coconut water) in MS media with 5 replicates with observation for 4 weeks after planting. The results showed did not differ markedly in the number of protocorms, number of shoots and number of browning protocorms, but differ markedly in the parameter protocorm color based on DMRT test. Treatment of 3 mg/l BAP gives the best results on the number of shoots 2,60 protocorms and number of browning protocormss at the least amount 0,80 protocorms. The number of protocorms most widely on the treatment of 1 g/l Growmore 14,40 protocorms. Protocorms color is best found in 3 g/l Growmore with green color. This research managed to multiply the number of protocorms and induces protocorms shoots from Grammatophyllum stapeliiflorum orchid.

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