scholarly journals In Vitro Screening Ketahanan Galur Padi (Oryza Sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Keracunan Besi (Fe)

2019 ◽  
Vol 19 (3) ◽  
pp. 241
Author(s):  
Onny Chrisna Pandu Pradana ◽  
Siti Novridha Andini
Keyword(s):  
Tissue Culture ◽  
Oryza Sativa ◽  
Iron Toxicity ◽  
In Vitro Screening ◽  
Culture Bottle ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
And Control

This research aimed to invstigate the response of paddy culture (B7 strain) assembled by Lampung State Polytechnic to the iron toxicity tolerance. The research was done at Plant Tissue Culture Laboratory, Lampung State Polytechnic, from July to September 2019. Treatments were single arranged in a completely randomized design with three replications. The treatment tried was five levels of Fe concentrations (5,6 ppm 28 ppm, 56 ppm, 84 ppm, 112 ppm, and control). Each replication consisted of three culture bottle containing one explant. The homogenity of data was tested using Barlett test. If the assumption were fulfilled then analysis of variance is executed using STATISTIX 10, and then followed by the Honest Significant Difference (HSD) test in 5% alpha for mean separation and RPA analysis. The result of this research showed that the B7 strain has tolerance to iron toxicity until 56 ppm of Fe concentration, it can be concluded from the PAR value of its strain (>0,50). Meanwhile in  84 and 112 ppm of Fe concentration, the RPA value of B7 starin (<0,50), and it is indicate that its strain is sensitive. 

scholarly journals Uji Virulensi Dua Isolat Ganoderma sp. terhadap Bibit Kelapa Sawit Kultur Jaringan di Laboratorium

2018 ◽  
Vol 1 (1) ◽  
pp. 6-10
Author(s):  
Mhd Irvan Fadli Nst ◽  
Lisnawita Lisnawita ◽  
Suzanna Sitepu
Keyword(s):  
Tissue Culture ◽  
Oil Palm ◽  
Growth And Development ◽  
Stem Rot ◽  
Culture In Vitro ◽  
Severity Of Disease ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control

Busuk pangkal batang (Ganoderma sp.) pada tanaman kelapa sawit merupakan penyakit utama yang dihadapi oleh perkebunan kelapa sawit di Indonesia. Di lapangan banyak faktor yang dapat mempengaruhi pertumbuhan dan perkembangan dari penyakit busuk pangkal batang dan penyakit ini menunjukkan serangan yang berbeda di setiap daerah. Penelitian ini bertujuan untuk melihat daya virulensi dari dua isolat Ganoderma sp. yang berasal dari daerah berbeda tanpa adanya faktor-faktor lain pada kelapa sawit kultur jaringan secara invitro. Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Sumatera Utara pada bulan Maret - Juli 2016. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan 3 perlakuan penginokulasian yaitu isolat Ganoderma sp.I, isolat Ganoderma sp.II, dan Kontrol. Hasil menunjukkan adanya perbedaan daya virulensi dari kedua isolat Ganoderma, dimana kejadian penyakit dan keparah penyakit tertinggi terjadi pada perlakuan Ganoderma isolat II. The stem rot (Ganoderma sp.) is the main disease faced by oil palm plantations in Indonesia. There are many factors that can affect the growth and development of stem rot in the different region. This study aims to study the virulence of two isolates of Ganoderma sp. originating from different regions in the absence of other factors in oil palm tissue culture in vitro. This study used a non-factorial Completely Randomized Design with 3 inoculation treatments, such as isolates I and II of Ganoderma sp., and Control. The results showed that there were differences in virulence power of the two Ganoderma sp. isolates, where the highest incidence of disease and severity of disease occurred in the treatment of isolates II of Ganoderma sp.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Dry Matter ◽  
Vegetable Waste ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

scholarly journals In vitro antimicrobial activity and alternative control of anthracnose in papaya

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Héber Ferreira dos Reis ◽  
Lilian Maria Arruda Bacchi ◽  
Silvana de Paula Quintão Scalon ◽  
Jasna Karoliny Pereira Flores
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Syzygium Aromaticum ◽  
Alternative Control ◽  
Ginger Extract ◽  
Cinnamomum Zeylanicum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control

ABSTRACT: We evaluated the efficacy of natural products in the control of papaya anthracnose, in vitro and in vivo. The in vitro experiments for evaluation of mycelial growth used a completely randomized 10 × 4 factorial design (treatments × evaluation periods) with eight replicates, with sporulation evaluated at the end of the experiment. The treatments involved the use of aqueous extract at concentrations of 5 and 15% for Syzygium aromaticum (L.) Merr. & Perry (clove), Cinnamomum zeylanicum Breym (cinnamon), and Zingiber officinalis Rox. (ginger); 1 and 3% chitosan; the fungicide Prochloraz at 100 µg.mL-1; and a control (no treatment). For evaluating conidia germination, we used six treatments with five replicates. The treatments included 7.5% of each extract (clove, cinnamon, and ginger), 1.5% chitosan, and 50 µg.mL-1 of Prochloraz. For the in vivo experiment, “Formosa” papaya “Tainung 1” was used in a completely randomized design, with six treatments and four replicates to evaluate the severity of anthracnose caused by Colletotrichum gloeosporioides. The fruits were treated by immersion for 5 min with 15% clove, cinnamon, and ginger extracts, 8% chitosan, and control with distilled water, and immersion for 2 min in Prochloraz (33.75 g a.i./100 L). The treatments with 15% clove extract and 8% chitosan were effective in all evaluations, resulting in a viable alternative to the fungicide Prochloraz. The treatments with ginger extract were less effective and those with cinnamon offered intermediate control.

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals Penambahan Glutathione pada Medium Fertilisasi Efektif Mendukung Pembentukan Pronukleus dan Perkembangan Awal Embrio Sapi (SUPPLEMENTATION OF GLUTATHIONE IN FERTILIZATION MEDIUM EFFECTIVELY SUPPORT NORMAL PRONUCLEUS FORMATION AND EARLY BOVINE EMBRYONIC DE

2017 ◽  
Vol 18 (3) ◽  
pp. 327
Author(s):  
Aras Prasetiyo Nugroho ◽  
Iman Supriatna ◽  
Mohamad Agus Setiadi
Keyword(s):  
Tissue Culture ◽  
Embryonic Development ◽  
Culture Medium ◽  
Fertilization Rate ◽  
Tissue Culture Medium ◽  
Randomized Design ◽  
Oviduct Fluid ◽  
And Control ◽  
Bovine Oocytes

The objective of this study was to determine fertilization rate effectiveness and early embryonic development competency with glutathione (GSH) supplementation in fertilization medium and culture This study consisted of two experiments comprising each of the four treatment and six repetitions with completely randomized design (CRD) using 651 oocytes. In the first experiment, a total of 317 bovine oocytes were matured in tissue culture medium (TCM) 199 at incubator 5% CO2 with temperature 39 ºC for24 h, then fertilized with sperm separated by swim up technique. Oocyte and sperms were incubated in fertilization medium supplemented with 0.25 mM, 0.50 mM, 1.00 mM GSH. In the second experiment, bovine oocytes were matured in maturation medium and fertilized with same procedure as mentioned before, then cultured in modified synthetic oviduct fluid (mSOF) with the following treatment: supplementation GSH only in fertilization medium (T1), supplementation GSH only in culture medium (T2), and supplementation GSH in both fertilization and culture medium (T3), while control not supplementation GSH (T0). Result of the first experiment showed that supplementation 1.00 mM GSH in fertilization medium can increase higher normal pronucleus (2PN) formation (86,9%) compared to other treatments, 0.50 mM (80.3%), 0.25 mM (73.8%), and control (58.9%) (P<0.05). In the second experiment showed that early bovine embryonic development on 2nd day cultured which reached 5-8 cell on treatment T1 (56.0%) and T3 (53.6%) were higher (P<0.05) compared to treatment T2 (26.2%) and T0 (control) (31.3%). Result of the other were showed that early bovine embryonic development on 4th day cultured which reached 9-16 cell on treatment T1 (26.2%) and T3 (27.4%) were higher (P<0.05) compared to that T2 (11.9%) and T0 (control) (10.8%). In conclusion, 1.00 mM GSH supplementation in medium was more effective in supporting normal pronucleus formation and early fertilization bovine embryonic development compared to in culture medium. ABSTRAK Penelitian ini bertujuan untuk mengetahui tingkat fertilisasi dan kompetensi perkembangan awal embrio sapi dengan penambahan glutathione (GSH) pada medium fertilisasi in vitro (IVF) dan kultur in vitro (IVC). Penelitian ini terdiri atas dua penelitian yang terdiri dari masing-masing empat perlakukan dan enam kali ulangan dengan rancangan acak lengkap (RAL) menggunakan 651 oosit. Penelitian I, sebanyak 317 oosit sapi dalam tissue culture medium (TCM) 199 dimatangkan pada inkubator 5% CO2 dan suhu 39°C selama 24 jam, kemudian difertilisasi dengan spermatozoa yang telah diseleksi menggunakan teknik swim up. Oosit dan spermatozoa diinkubasi pada medium fertilisasi dengan penambahan 0,25 mM, 0,50 mM, dan 1,00 mM GSH. Penelitian II, sebanyak 334 oosit sapi dimatangkan pada medium pematangan dan difertilisasi, kemudian dikultur pada medium modified synthetic oviduct fluid (mSOF), dengan perlakuan: penambahan GSH hanya pada medium fertilisasi (T1), penambahan GSH hanya pada medium kultur (T2), dan kombinasi penambahan GSH pada medium fertilisasi dan kultur (T3). Hasil penelitian I, menunjukkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi dapat meningkatkan pembentukan pronukleus normal (2PN) yang lebih tinggi (86,9%) dibandingkan dengan perlakuan yang lain yaitu 0,50 mM (80,3%), 0,25 mM (73,8%), dan kontrol (58,9%) (P<0,05). Penelitian II menujukkan bahwa perkembangan awal embrio sapi pada hari ke-2 kultur yang mencapai pembelahan 5-8 sel pada perlakukan T1 (56,0%) dan T3 (53,6%) lebih tinggi (P<0,05) dibandingkan dengan perlakuan T2 (26,2%) dan T0 (kontrol) (31,3%). Hasil penelitian lain menunjukkan bahwa perkembangan awal embrio sapi pada hari ke-4 kultur yang mencapai pembelahan 9-16 sel pada perlakuan T1 (26,2%) dan T3 (27,4%) lebih tinggi dibandingkan dengan perlakukan T2 (11,9%) dan T0 (kontrol) (10,8%) (P<0,05). Dapat disimpulkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi lebih efektif dalam mendukung pembentukan pronukleus normal dan perkembangan awal embrio sapi dibandingkan pada medium kultur.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽  
10.15575/1344 ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 97-109
Author(s):  
Lamro Purba ◽  
Erni Suminar ◽  
Denny Sobardini ◽  
Wieny Rizky ◽  
Syariful Mubarok
Keyword(s):  
Tissue Culture ◽  
Leaf Number ◽  
Shoot Induction ◽  
Seed Technology ◽  
Randomized Design ◽  
Rooting Media ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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