Identification and Antimicrobial Activity of Marine Streptomyces from Geographically Different Regions of Indonesia

Identification and antimicrobial assay of indegenous marine Streptomyces have been conducted. Samples were obtained from culture collection of Biotech Center, Agency for the Assessment and Application of Technology (BPPT). They were originated from several Indonesian seashores including Pelabuhan Ratu, Pangandaran, Manokwari, Pulau Seribu, Garut, Bangka, Banjarmasin, Belitung, Cirebon, and Palu. Isolates stored as glycerol stocks were inoculated onto HV (Humic acid vitamin) agar and incubated for 5 days at 30 °C. Each colony was cultivated using yeast-peptone medium and then extracted by butanol. Antimicrobial activities were monitored by the agar diffusion paper-disc method against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, Pseudomonas aeruginosa ATCC 27853, Candida albicans BIOMCC 00122 and Aspergillus niger BIOMCC 0013. Molecular identification of Streptomyces was carried out based on 16S rRNA gene analysis. Our research results showed that 71 isolates obtained from several Indonesian seashore were identified as 57 different Streptomyces species. Fifty of them showed antimicrobial activity. Twenty three isolates inhibited B. subtilis ATCC 66923, 14 isolates inhibited S. aureus ATCC 25923, 24 isolates inhibited C. albicans BIOMCC 00122 and 26 isolates inhibited A. niger BIOMCC 0013 and there was no active isolates inhibited the growth of E. coli ATCC 25922 and P. aeruginosa ATCC 27853. Phylogenetic analysis showed that Streptomyces isolates originated from the same geographically region was not necessarily grouped into the same cluster. Likewise a phylogenetic cluster may contain isolates of the same Streptomyces species, but from geographically different locations.

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ANTIMICROBIAL ACTIVITY OF STREPTOMYCES SP. PJ90 ISOLATED FROM SOIL IN NORTHEAST THAILAND

Jurnal Teknologi ◽

10.11113/jt.v78.8574 ◽

2016 ◽

Vol 78 (5-5) ◽

Author(s):

Panjamaphon Chanthasena ◽

Nawarat Nantapong

Keyword(s):

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Staphylococcus Epidermidis ◽

Morphological Characteristics ◽

Rrna Gene ◽

Northeast Thailand ◽

16S Rrna Gene Analysis ◽

Streptomyces Sp ◽

University Of Technology

In this study, an antimicrobial-producing Actinomycetes PJ90 was isolated from forest soil in Suranaree University of Technology, Nakhon Ratchasima, Thailand. The morphological characteristics and 16S rRNA gene analysis revealed that isolate PJ90 could be classified as Streptomyces triostinicus. The isolate PJ90 exhibited antimicrobial activity against Staphylococcus aureus TISTR1466, Staphylococcus epidermidis TISTR518, Bacillus subtilis TISTR008, Candida albicans TISTR5779, Candida tropicalis TISTR5174 and Saccharomyces cerevisiae TISTR5049. To our best knowledge, this study constitutes the first anti-bacterial and anti-yeast activities of Streptomyces triostinicus isolated from soil in Thailand.

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Characterization and Antimicrobial Activities of a Metabolite from a New Streptomyces Species from Bangladeshi Soil

Journal of Scientific Research ◽

10.3329/jsr.v2i1.3079 ◽

2009 ◽

Vol 2 (1) ◽

pp. 178-185

Author(s):

M. Akhand ◽

M. A. A. Al-Bari ◽

M. A. Islam ◽

Proma Khondkar

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Activities ◽

Assay Method ◽

Streptomyces Species ◽

Isolation And Characterization ◽

Test Organisms ◽

Actinomycete Strain ◽

Diffusion Assay ◽

Strong Antimicrobial Activity

A new actinomycete strain was isolated from Western part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. The present paper describes the isolation and characterization of compound 1 from this new Streptomyces species with the help of various chemical and spectroscopic methods. Antimicrobial activity of compound 1 was tested by disc diffusion assay method and compared with that of standard antibiotics (Kanamycin for antibacterial activity and Nystatin for antifungal activity). The compound has been found to exhibit moderate to strong antimicrobial activity against the test organisms. Cytotoxicity of the compound 1 and the pet. ether extract of Czapek Dox (alkaline) broth of Streptomyces species was evaluated in brine shrimp bioassay with LC50 values of 23.85 µg/ml (ppm) and 19.95 µg/ml (ppm), respectively. Keywords: Streptomyces; Antimicrobial activity; Cytotoxicity. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.3079 J. Sci. Res. 2 (1), 178-185 (2010)

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Antimicrobial Activity of New 2,4-Disubstituted Thiazolidinone Derivatives

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-11-1205 ◽

2009 ◽

Vol 64 (11-12) ◽

pp. 785-789 ◽

Cited By ~ 5

Author(s):

Wael A. El-Sayed ◽

Yasser K. Abdel-Monem ◽

Nabil M. Yousif ◽

Nashwa Tawfek ◽

Mohamed T. Shaaban ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Antimicrobial Activities ◽

Gram Positive ◽

Streptomyces Species ◽

Gram Negative

A number of new disubstituted 2,5-thiazolidinone derivatives were synthesized and tested for their antimicrobial activity against Bacillus subtilis (Gram-positive), Pseudomonas aeruginosa (Gram-negative), and Streptomyces species (Actinomycetes). They displayed different degrees of antimicrobial activities or inhibitory actions

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Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria

Jurnal Sumberdaya Hayati ◽

10.29244/jsdh.3.1.21-26 ◽

2017 ◽

Vol 3 (1) ◽

pp. 21-26

Author(s):

SASMIATI FARACH DITA ◽

SRI BUDIARTI ◽

YULIN LESTARI

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Marine Ecosystem ◽

Morphological Characteristics ◽

Antibacterial Activities ◽

Morphological Characters ◽

Antimicrobial Activities ◽

Shigella Dysenteriae ◽

Rrna Gene

Sponge-associated actinobacteria may diverse and have potency to produce bioactive compounds. Diversity and antimicrobial activity of indigenous sponge-associated actinobacteria isolated from the marine ecosystem in Indonesia have not much been explored. This work aimed to assess morphological and antibacterial activity of sponge-associated actinobacteria. The morphological characteristics were examined based on their color of aerial and substrate mycelia, and pigmentation, while antibacterial activities were assayed using the antagonist technique. The selected actinobacterial isolate was identified using 16S rRNA gene. Various sponge-associated actinobacteria were successfully isolated from Hyrtios sp., Callyspongia sp., and Neofibularia sp. sponges. A total of 62 actinobacterial isolates were obtained, and each isolate showed a variety of morphological characters, which could be seen in aerial mass color, substrate mass color, and pigmentation. Actinobacterial isolates were tested against human pathogenic bacteria, i.e. Staphylococcus aureus and Methicillin-Resistant S. aureus, representing Gram-positive, and Escherichia coli EPEC K1-1 and Shigella dysenteriae, representing Gram-negative. Most of actinobacterial isolates had antimicrobial activities at least against one of pathogenic bacteria. High activity was shown by NOHa.2, isolated from Neofibularia, and HRHa.5 isolated from Hyrtios. The NOHa.2 showed the highest antimicrobial activity against S. dysenteriae, meanwhile, HRHa.5 showed antimicrobial activity against 3 of 4 tested bacterial pathogens. These data showed diversity of sponge-asccociated actinobacteria from marine ecosystem in Indonesia, and several of them have potency as source of antibacterial compounds

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Probiotic Potential ofLactobacillusStrains with Antimicrobial Activity against Some Human Pathogenic Strains

BioMed Research International ◽

10.1155/2014/927268 ◽

2014 ◽

Vol 2014 ◽

pp. 1-16 ◽

Cited By ~ 77

Author(s):

Parisa Shokryazdan ◽

Chin Chin Sieo ◽

Ramasamy Kalavathy ◽

Juan Boo Liang ◽

Noorjahan Banu Alitheen ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Activities ◽

Rrna Gene ◽

Human Pathogens ◽

Bacterial Strains ◽

Probiotic Potential ◽

Good Ability ◽

Acid And Bile Tolerance ◽

Infant Feces

The objective of this study was to isolate, identify, and characterize some lactic acid bacterial strains from human milk, infant feces, and fermented grapes and dates, as potential probiotics with antimicrobial activity against some human pathogenic strains. One hundred and forty bacterial strains were isolated and, after initial identification and a preliminary screening for acid and bile tolerance, nine of the best isolates were selected and further identified using 16 S rRNA gene sequences. The nine selected isolates were then characterizedin vitrofor their probiotic characteristics and their antimicrobial activities against some human pathogens. Results showed that all nine isolates belonged to the genusLactobacillus. They were able to tolerate pH 3 for 3 h, 0.3% bile salts for 4 h, and 1.9 mg/mL pancreatic enzymes for 3 h. They exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics. They also showed good antimicrobial activities against the tested pathogenic strains of humans, and most of them exhibited stronger antimicrobial activity than the reference strainL. caseiShirota. Thus, the nineLactobacillusstrains could be considered as potential antimicrobial probiotic strains against human pathogens and should be further studied for their human health benefits.

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Synthesis and Antimicrobial Activity of New Substituted 1,2,4-Triazoles and their Acyclic C-Nucleoside Analogues

Zeitschrift für Naturforschung C ◽

10.1515/znc-2010-1-203 ◽

2010 ◽

Vol 65 (1-2) ◽

pp. 15-21 ◽

Cited By ~ 3

Author(s):

Wael A. El-Sayed ◽

Omar M. Ali ◽

Saly R. El-Dakkony ◽

Adel A.-H. Abdel-Rahman

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Antimicrobial Activities ◽

Nucleoside Analogues ◽

Gram Positive ◽

Streptomyces Species ◽

Gram Negative ◽

Sugar Hydrazones

A number of new substituted 1,2,4-triazole {[(1,2,4-triazolyl)ethyl]tetrazolyl} derivatives, their sugar hydrazones, and their acyclic C-nucleoside analogues were synthesized and tested for their antimicrobial activity against Bacillus subtilis (Gram-positive), Pseudomonas aeruginosa (Gram-negative), and Streptomyces species (Actinomycetes). The synthesized compounds displayed different degrees of antimicrobial activities or inhibitory actions.

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Taxonomy and antimicrobial activities of a new Streptomyces sp. TN17 isolated in the soil from an oasis in Tunis

Archives of Biological Sciences ◽

10.2298/abs1104047s ◽

2011 ◽

Vol 63 (4) ◽

pp. 1047-1056 ◽

Cited By ~ 5

Author(s):

Slim Smaoui ◽

Florence Mathieu ◽

Ben Fguira ◽

Georges Merlina ◽

Lotfi Mellouli

Keyword(s):

Taxonomic Status ◽

Antimicrobial Activities ◽

Rrna Gene ◽

Streptomyces Species ◽

Streptomyces Sp ◽

Phenotypic Differences ◽

Fatty Acids Profile ◽

Actinomycete Strain ◽

Bacteria And Fungi ◽

Physiological Tests

An actinomycete strain referred to as TN17 was screened for its antimicrobial activities. The taxonomic status of this strain was established. The organism was found to have morphological and chemotaxonomic characteristics typical of Streptomycetes. Based on the 16S rRNA nucleotide sequences, Streptomyces sp. TN17 was found to have a relationship with Streptomyces lilaceus, Streptomyces gobitricini and Streptomyces lavendofoliae. Combined analysis of the 16 S rRNA gene sequence (FN687757), phylogenetic analysis, fatty acids profile and physiological tests indicated that there are genotypic and phenotypic differences between TN17 and neighboring Streptomyces species? neighbors. Therefore, TN17 is a novel species: Streptomyces sp. TN17 (=DSM 42020T=CTM50229T). A cultured extract of this strain inhibits the growth of several Gram positive and Gram negative bacteria and fungi.

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Antimicrobial Activities of Chitosan Derivatives

Pharmaceutics ◽

10.3390/pharmaceutics13101639 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1639

Author(s):

Cristina Ardean ◽

Corneliu Mircea Davidescu ◽

Nicoleta Sorina Nemeş ◽

Adina Negrea ◽

Mihaela Ciopec ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Activities ◽

Culture Collection ◽

Quaternary Ammonium Salts ◽

Ammonium Salts ◽

New Materials ◽

Direct Control ◽

Antifungal Effects ◽

Antibacterial And Antifungal ◽

Reference Strains

Considering the challenge created by the development of bacterial and fungal strains resistant to multiple therapeutic variants, new molecules and materials with specific properties against these microorganisms can be synthesized, like those synthesized from biopolymers such as chitosan with improved antimicrobial activities. Antimicrobial activities of seven obtained materials were tested on four reference strains belonging to American Type Culture Collection. The best antimicrobial activity was obtained by functionalization by impregnation of chitosan with quaternary ammonium salts, followed by that obtained by functionalization of chitosan with phosphonium. The lowest antibacterial and antifungal effects were expressed by Ch-THIO and Ch-MBT, but new materials obtained with these extractants may be precursors with a significant role in the direct control of active molecules, such as cell growth factors or cell signaling molecules.

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Synthesis and Antimicrobial Activity of New Substituted Fused 1,2,4-Triazole Derivatives

Zeitschrift für Naturforschung C ◽

10.1515/znc-2010-1-204 ◽

2010 ◽

Vol 65 (1-2) ◽

pp. 22-28 ◽

Cited By ~ 3

Author(s):

Wael A. El-Sayed ◽

Omar M. Ali ◽

Marwa M. Hathoot ◽

Adel A.-H. Abdel-Rahman

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Antimicrobial Activities ◽

Gram Positive ◽

Streptomyces Species ◽

Triazole Derivatives

A number of new substituted 1,2,4-triazole, 1,2,4-triazolo[3,4-b]1,3,4-thiadiazole and 1,2,4-triazolo[3,4-b]1,3,4-thiadiazine derivatives were synthesized and tested for their antimicrobial activity against Bacillus subtilis (Gram-positive), Pseudomonas aeruginosa (Gramnegative), and Streptomyces species (Actinomycetes). The synthesized compounds displayed different degrees of antimicrobial activities or inhibitory actions.

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Antimicrobial Characteristics of Lactic Acid Bacteria Isolated from Homemade Fermented Foods

BioMed Research International ◽

10.1155/2018/5416725 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Dayong Ren ◽

Jianwei Zhu ◽

Shengjie Gong ◽

Hongyan Liu ◽

Hansong Yu

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Proteinase K ◽

Control Group ◽

Rrna Gene ◽

Fermented Foods ◽

16S Rrna Gene Analysis ◽

Group 10

Objective. Lactic acid bacteria (LAB) were isolated from fermented foods, such as glutinous rice dough, corn noodle, chili sauce, potherb mustard pickles, and stinky tofu, in northeast China. LAB strains with antimicrobial activities were screened, and seven of these Lactobacillus strains were identified as L. plantarum, L. pentosus, and L. paracasei through 16S rRNA gene analysis. After the supernatant of LAB was treated with proteinase K, pepsin, and papain, their antibacterial effect almost disappeared. Most strains with antibacterial activities were highly resistant to heat (65°C–121°C), acidity (pH 2–6), and alcohol. The antimicrobial effect of most strains treated with the Tween-80 surfactant was significantly reduced, and the antibacterial property of T4 was even lost. Ammonium sulfate precipitation, PCR, and nanoLC-ESI-MS/MS results confirmed that T8 produced antibacterial substances belonging to a protein family, and its zone of inhibition against pathogens significantly increased (>13 mm). In bacterial growth inhibition experiments, the colony count of Staphylococcus aureus was up to 1015 CFU/mL in the 3⁎de Man, Rogosa, and Sharpe (MRS) group, and this value was more than that in the 3⁎S6 supernatant group (1012 CFU/mL) and the control group (1010 CFU/mL) at 12 h. This study provided a basis for the selection of antimicrobial peptides and the development and utilization of LAB.

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