scholarly journals Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria

2020 ◽  
Vol 15 (2) ◽  
pp. 81
Author(s):  
Sri Sedjati ◽  
Ambariyanto Ambariyanto ◽  
Agus Trianto ◽  
Endang Supriyantini ◽  
Ali Ridlo ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Antibacterial Activities ◽  
Drug Resistant ◽  
Antibacterial Compounds ◽  
Trichoderma Longibrachiatum ◽  
Layer Chromatography ◽  
Antibacterial Potential ◽  
Ethyl Acetate Extracts

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.  Meanwhile, the  ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria

Antibacterial Activities of Ramalin, Usnic Acid and its Three Derivatives Isolated from the Antarctic Lichen Ramalina terebrata

2010 ◽  
Vol 65 (1-2) ◽  
pp. 34-38 ◽  
Author(s):  
Babita Paudel ◽  
Hari Datta Bhattarai ◽  
Hong Kum Lee ◽  
Hyuncheol Oh ◽  
Hyun Woung Shin ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Usnic Acid ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibacterial Compounds ◽  
The Antarctic ◽  
Antibacterial Potential

The development of new antibacterial compounds is an urgent issue to meet the evolution of resistivity of pathogenic bacteria against the available drugs. The objective of this study was to investigate the antibacterial compounds from the Antarctic lichen species Ramalina terebrata. A total of fi ve compounds, usnic acid, usimine A, usimine B, usimine C, and ramalin, were isolated by bioactivity guided-fractionation of the methanol extract of R. terebrata after several chromatographic procedures. The qualitative antibacterial activities of the crude extract and isolated compounds were determined by the disk diffusion method while the minimum inhibitory concentration (MIC) determination assay gave the quantitative strength of the test samples. All the test samples showed antibacterial activity against Bacillus subtilis. The crude extract and usnic acid showed antibacterial activity against Staphylococcus aureus. The MIC values of the isolated compounds against B. subtilis were in the range of 1 to 26 μg/mL. These observed experimental data showed the strong antibacterial potential of these compounds against B. subtilis.

scholarly journals Antibacterial Compounds from Mushrooms: A Lead to Fight ESKAPEE Pathogenic Bacteria?

Planta Medica ◽  
2020 ◽  
Author(s):  
Violette Hamers ◽  
Clément Huguet ◽  
Mélanie Bourjot ◽  
Aurélie Urbain
Keyword(s):  
Antibiotic Resistance ◽  
Global Health ◽  
Pathogenic Bacteria ◽  
Resistance Mechanisms ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Antibacterial Compounds ◽  
New Antibiotics ◽  
Hospital Stays ◽  
Antibacterial Potential

AbstractInfectious diseases are among the greatest threats to global health in the 21st century, and one critical concern is due to antibiotic resistance developed by an increasing number of bacterial strains. New resistance mechanisms are emerging with many infections becoming more and more difficult if not impossible to treat. This growing phenomenon not only is associated with increased mortality but also with longer hospital stays and higher medical costs. For these reasons, there is an urgent need to find new antibiotics targeting pathogenic microorganisms such as ESKAPEE bacteria. Most of currently approved antibiotics are derived from microorganisms, but higher fungi could constitute an alternative and remarkable reservoir of anti-infectious compounds. For instance, pleuromutilins constitute the first class of antibiotics derived from mushrooms. However, macromycetes still represent a largely unexplored source. Publications reporting the antibacterial potential of mushroom extracts are emerging, but few purified compounds have been evaluated for their bioactivity on pathogenic bacterial strains. Therefore, the aim of this review is to compile up-to-date data about natural products isolated from fruiting body fungi, which significantly inhibit the growth of ESKAPEE pathogenic bacteria. When available, data regarding modes of action and cytotoxicity, mandatory when considering a possible drug development, have been discussed in order to highlight the most promising compounds.

Preparation of graphene oxide (GO)/lanthanum coordination polymers for enhancement of bactericidal activity

2021 ◽  
Author(s):  
Jia Wang ◽  
Zezhi Shan ◽  
Xiao Tan ◽  
Xinxiang Li ◽  
Zhenqi Jiang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Coordination Polymers ◽  
Bactericidal Activity ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Large Scale ◽  
Antibacterial Activities ◽  
Antibacterial Compounds ◽  
Large Scale Preparation ◽  
Scale Preparation

The synthesized GO/lanthanum coordination polymers exhibit broad-spectrum antibacterial activities against seven typical Pathogenic bacteria, are compatible with large-scale preparation and can be harnessed as antibacterial compounds.

Patulin Production in Apples Stored in a Controlled Atmosphere

1975 ◽  
Vol 58 (5) ◽  
pp. 912-914 ◽  
Author(s):  
Joseph Lovett ◽  
Ruben G Thompson ◽  
Brenda K Boutin
Keyword(s):  
Carbon Dioxide ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Penicillium Expansum ◽  
Controlled Atmosphere ◽  
Atmospheric Conditions ◽  
Naturally Occurring ◽  
Apple Tissue ◽  
Layer Chromatography ◽  
Ethyl Acetate Extracts

Abstract Red Delicious apples were inoculated with Penicillium expansum NRRL 973 or P. expansutn 1071 (fresh apple isolate), and incubated in air at 33°F and in a controlled atmosphere of 1% carbon dioxide, 3% oxygen, and 96% nitrogen at 33°F. Both fungal strains produced the carcinogen, patulin, in the air-incubated lots, but only the freshly isolated strain (1071) produced detectable patulin in controlled atmosphere lots. Thin layer chromatography was used to assay ethyl acetate extracts of juice pressed from blended apple tissue. We conclude that naturally occurring P. expansum strains are capable of producing significant levels of patulin in apples stored from 3 to 6 months under controlled atmospheric conditions.

Diversity and antibacterial activity of actinomycetes from wetland soil

2010 ◽  
Vol 28 (1) ◽  
pp. 52 ◽  
Author(s):  
Maya George ◽  
Gisha George ◽  
A. A. Mohamed Hatha
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Enzyme Production ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Wetland Soil ◽  
Production Potential ◽  
Soil Actinomycetes ◽  
Test Organisms ◽  
Antibacterial Potential

Diversity of soil actinomycetes isolated from various regions of a wetland was analysed. The strains were assigned under 11 genera according to their morphological and biochemical characteristics. They are: Nocardia, Pseudonocardia, Streptomyces, Micromonospora, Rhodococcus, Actinosynnema, Nocardiodes, Kitasatospora, Gordona, Intrasporangium and Streptoalloteichus. Around 55% of the identified strains are Nocardioform actinomycetes. Screening for their antibacterial activities revealed that 96% of the isolated strains showed different degrees of inhibition potential against 12 test pathogenic bacteria (including various serotypes of Salmonella, Vibrio cholerae, Bacillus subtilis and Escherichia coli). Of these about 41% of strains showed antagonism towards Bacillus subtilis. The Nocardioform actinomycetes exhibited antibacterial activity against 9 out of 12 test organisms. The least antibacterial potential was exhibited by Gordona, Rhodococcus, Micromonospora and Kitasatospora. The isolated strains differed among themselves in their ability to decompose proteins and amino acids and also in enzyme production potential.

scholarly journals THÀNH PHẦN VÀ HOẠT TÍNH KHÁNG KHUẨN CỦA CAO ETYL AXETAT CHIẾT TỪ 3 LOÀI RONG ĐỎ THUỘC CHI LAURENCIA

2018 ◽  
Vol 18 (2) ◽  
pp. 187-196
Author(s):  
Le Ho Khanh Hy ◽  
Nguyen Kim Hanh ◽  
Pham Xuan Ky ◽  
Dao Viet Ha ◽  
Nguyen Thu Hong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Salmonella Typhimurium ◽  
Antibacterial Activities ◽  
Ethyl Acetate Extracts

This paper is concerned with composition and antibacterial activities of ethyl acetate extracts from 3 species of Laurencia (Rhodophyta): Laurencia papillosa, Laurencia flexilis and Laurencia cartilaginea collected from the southern coasts of Vietnam. The results showed that ethyl acetate (EtOAc) extracts of these species displayed antibacterial activities against Salmonella typhimurium, Escherichia coli and Bacillus subtilis. The qualitative investigations revealed the presence of two sesquiterpene secondary metabolites Elatol and Palisadin A in EtOAc extracts from the three above- mentioned Laurencia species.

scholarly journals Antibacterial and antifungal activity of crude extracts of Plumeria rubra L.

2013 ◽  
pp. 87-94
Author(s):  
Laila Jarin ◽  
Md Shafiqur Rahman ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts ◽  
Plumeria Rubra

Petroleum ether, carbon tetrachloride, chloroform and ethyl acetate extracts of Plumeria rubra leaves were studied for their antimicrobial activities against eleven human pathogenic bacteria, viz., Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae and four human pathogenic fungi, viz., Aspergillus niger, A. ochraceus, A. ustus and Candida albicans using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extract exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extracts exhibited the largest zone of inhibition (25 mm in diameter with 2000 mg/disc extract) against E. coli. The highest inhibition of fungal radial mycelial growth (62.00% with 100 mg extract/ml medium) was recorded against A. ustus with ethyl acetate extract. The MICs were determined by broth macrodilution technique. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against E. coli. However, for fungi the lowest MIC was 500 mg/ml against A. ustus with the same extract. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13409 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):87-94, 2008

scholarly journals Bioproduction of Sorbicillin Derivatives from Marine Trichoderma sp.

2008 ◽  
Vol 63 (9-10) ◽  
pp. 631-635 ◽  
Author(s):  
Ahmed Abdel-Lateff
Keyword(s):  
Ethyl Acetate ◽  
Biomass Production ◽  
Convenient Method ◽  
Culture Media ◽  
Diode Array ◽  
Diode Array Detection ◽  
Trichoderma Sp ◽  
Array Detection ◽  
Good Medium ◽  
Ethyl Acetate Extracts

The variability of sorbicillin derivatives production by marine Trichoderma sp., isolated from the sponge Agelas dispar J., was studied using six different culture media to find a good medium for biomass production, particularly of trichodimerol, bislongiquinolide and bisvertinol. A simple and rapid convenient method for identification, quantification and validation of the sorbicillin derivatives in ethyl acetate extracts has been applied by using RPHPLC coupled with diode array detection.

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