scholarly journals Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity

2013 ◽  
Vol 8 (2) ◽  
pp. 69 ◽  
Author(s):  
Kustiariyah Tarman ◽  
Dwi Safitri ◽  
Iriani Setyaningsih
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Culture Broth ◽  
Rhizophora Mucronata ◽  
Economic Potential ◽  
Mangrove Plant ◽  
Traditional Medicines ◽  
Activity Test ◽  
Fungal Extracts

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.

scholarly journals Antibacterial Activity Test of Endophytic Fungus from Mangrove Plant (Rhizophora apiculata L.) and (Bruguiera gymnorrizha (L.) Lamk.) Against Klebsiella pneumoniae ATCC 700603

2017 ◽  
Vol 2 (6) ◽  
pp. 146
Author(s):  
Rossiana Nia ◽  
Miranti Mia ◽  
Kosmita Oktapiana
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Drug Candidate ◽  
Mangrove Plant ◽  
Fungal Extract ◽  
Rhizophora Apiculata ◽  
Activity Test

Pathogenic bacteria Klebsiella pneumoniae is the cause of pneumonia disease in infants and adults. Antibiotics treatment is usually used to treat this disease. However, synthetic antibiotic use could lead to bacterial resistance. Therefore, novel antibiotics from natural resources are required to overcome this problem. The aim of present study is to determine the antibacterial activity of endophytic fungal extracts from mangrove plants Rhizophora apiculata and Bruguiera gymnorrhiza against K. pneumonia ATCC 700 603. Isolation, cultivation, extraction and antibacterial activity test were conducted. Fourteen endophytic fungi were derived from the two mangrove species, where the two fungal isolates: Penicilium sp.2 and Penicilium sp.4 showed the highest antibacterial activity. The minimum inhibitory concentration of K. pneumoniae for both fungal extract is 3.6 mg mL-1, where bactericidal effect was observed. Additionally, antibacterial activity from the extract of Penncillium sp.4 is higher compared to Penicilium sp.2 extract, which is signified by larger average diameter of inhibition zone. However, at concentration more than 3.6 mg mL-1, both extract effectively inhibit the growth of K. pneumoniae. Our study reveals the potential of fungal extract from mangrove plant as prospective drug candidate to overcome the pneumonia disease. Keywords: antibacterial, pneumonia, endophytic fungi, mangrove

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDOFIT DAUN MANGROVE AVICENNIA MARINA TERHADAP BAKTERI UJI STAPHYLOCOCCUS AUREUS DAN SHIGELLA DYSENTERIAE

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Yolanda A. Kasi ◽  
Jimmy Posangi ◽  
O. Mona Wowor ◽  
Robert Bara
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
Avicennia Marina ◽  
Shigella Dysenteriae ◽  
Plant Tissues ◽  
Mangrove Plant ◽  
Activity Test ◽  
White Mycelium

Abstract: Endophytic fungi is a species of fungi which lives within plant tissues including leaf’s tissues that also harmless for the host plant itself. Isolates of endophytic fungi have a potent antibacterial capability with broad spectrum. The aim of this research is to know whether there is an antibacterial effect of endophytic fungi which isolated from Avicennia marina leaf towards Staphylococcus aureus and Shigella dysenteriae. The method that is used for the antibacterial activity test, done by putting mycelia of endophytic fungi in combination media that has been smeared with Staphylococcus aureus and Shigella dysenteriae. Based on research that has been done, it obtained two types of endophytic fungi isolated from mangrove plant, Avicennia marina leaf. Both endophytic fungi have potential for inhibiting Staphylococcus aureus and Shigella dysenteriae, but endophytic fungi with white mycelium has a better antibacterial effect than endophytic fungi with black mycelium and positive control.Keywords: antibacterial, endophytic fungi, mangrove avicennia marinaAbstrak: Jamur endofit merupakan jamur yang hidup di dalam jaringan tanaman salah satunya yaitu dalam jaringan daun dan tidak membahayakan tanaman tersebut. Isolat-isolat jamur endofit memiliki daya antibakteri yang poten dengan spektrum luas. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antibakteri jamur endofit daun Avicennia marina terhadap bakteri Staphylococcus aureus dan Shigella dysenteriae. Metode yang digunakan untuk uji antibakteri yaitu dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan, diperoleh dua jenis jamur endofit yang diisolasi dari daun Mangrove Avicennia marina. Kedua jamur endofit memiliki potensi dalam menghambat kedua bakteri uji tetapi jamur endofit miselium putih memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit miselium hitam dan kontrol positif.Kata kunci: antibakteri, jamur endofit, daun avicennia marina

scholarly journals Antibacterial Activity of Ethyl Acetate Culture Broth Extract from Endophytic Fungi of Nigrospora oryzae Associated with Rhizophora mucronata

2017 ◽  
Vol 15 (2) ◽  
pp. 191
Author(s):  
Muhamad S. Fareza ◽  
Lingling T. Ayoesty ◽  
Siti R. Wargiyanti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Culture Broth ◽  
Dilution Method ◽  
Rhizophora Mucronata ◽  
Nigrospora Oryzae ◽  
Mic Value

The purpose of this study was to provide information on the antibacterial activity of ethyl acetate culture broth extract of endophytic fungi associated with Rhizophora mucronata. An isolated fungi was identifi ed as Nigrospora oryzae using microscopic and molecular analysis. Theantibacterial activity was carried out using micro dilution method to get MIC value. The ethyl acetate extract showed antibacterial activity with MIC value of 250 μg/mL against and Escherichia coli and 500 μg/mL against Staphylococcus aureus.

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDIFIT PADA DAUN MANGROVE Sonneratia alba TERHADAP BAKTERI UJI Staphylococcus aureus DAN Escherichia coli

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwilestari . ◽  
Henoch Awaloei ◽  
Jimmy Posangi ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
Type I ◽  
Mangrove Plant ◽  
Activity Test ◽  
Sonneratia Alba ◽  
Tissue System

Abstract: Endophytic fungi is a species of fungi which life on plant tissue system. Endophytic fungi can be isolated from the roots, stems and leave of the plant. Endophytic fungi can produce a substance potencial to be antibacteria. This research aimed to test the presence of antibacterial effect on an isolated leaf of mangrove plant Sonneratia alba towards Staphylococcus aureus and Escherichia coli. The method that is used for the antibacterial activity test, done by putting mycelia of endophytic fungi in combination media that has been smeared with Staphylococcus aureus and Escherichia coli. Obtained from research that has been made, it conducted two species of endophytic fungi that isolated from mangrove plant leaf Sonneratia alba. Both endophytic fungi has effect as antibacteria against Staphylococcus aureus and Escherichia coli, but the second type of endophytic fungi has more effective and stronger antibacterial effect compare to endophytic fungi type I and positive control.Keywords: antibacterial, endophytic fungi, mangrove Sonneratia albaAbstrak: Jamur endofit adalah jamur yang terdapat dalam sistem jaringan tumbuhan. Jamur endofit dapat diisolasi dari akar, batang dan daun tumbuhan. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antibakteri jamur endofit daun Sonneratia alba terhadap bakteri Staphylococcus aureus dan Escherichia coli. Metode yang digunakan untuk uji antibakteri dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan diperoleh dua jenis jamur endofit yang diisolasi dari daun tumbuhan mangrove Sonneratia alba. Kedua jamur endofit memiliki efek sebagai antibakteri terhadap kedua bakteri uji akan tetapi jamur endofit tipe II memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit tipe I dan kontrol positif.Kata kunci: antibakteri, jamur endofit, daun Sonneratia alba

scholarly journals The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots Avicennia marina as a Producer of Antibiotics

2019 ◽  
Vol 6 (1) ◽  
pp. 83
Author(s):  
Kustiasih Lestari ◽  
Anthoni Agustien ◽  
Akmal Djamaan
Keyword(s):  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Avicennia Marina ◽  
Antibiotic Activity ◽  
Microbiology Laboratory ◽  
Inhibitory Zone ◽  
Fungal Isolation ◽  
Activity Test ◽  
Mangrove Roots ◽  
Source Of Information

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.

scholarly journals ISOLATION OF ACTINOMYCIN D FROM MARINE DERIVED Streptomyces

2010 ◽  
Vol 7 (3) ◽  
pp. 350-353
Author(s):  
Abdullah Rasyid ◽  
Kyoko Adachi
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Marine Bacterium ◽  
Actinomycin D ◽  
Culture Supernatant ◽  
Culture Broth ◽  
Bacterial Cells ◽  
Antibacterial Screening ◽  
Activity Test ◽  
Bioactive Substance

The bioactive substance producing strain, a marine bacterium A5S-46 was found in the antibacterial screening. This strain was isolated from the artificial sponge set in the coastal seawater at Iriomote islands (Japan). The crude extract from culture broth of the strain A5S-46 was assayed to the antibacterial activity test against the seven kinds of bacterial test strains. The active substance accumulated in the both bacterial cells and culture supernatant. Based on the 1H NMR and the LC/PDA/MS data, the bioactive substance was identified to be actinomycin D.   Keywords: Marine bacteria, Actinomycin D, Antibacterial, Anticancer

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

2020 ◽  
Vol 840 ◽  
pp. 205-213
Author(s):  
Elfita Elfita ◽  
Muharni Muharni ◽  
Mardiyanto Mardiyanto ◽  
Fitrya Fitrya ◽  
Feti Fera ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Antibacterial Compounds ◽  
Activity Test ◽  
Antibacterial Compound ◽  
Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

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