Construction of vector and transformation of drought-responsive gene OsNAC1 into J02 (Oryza sativa L. Japonica) rice

NAC (including NAM - no apical meristem, ATAF1/2 - Arabidopsis transcription activation factor and CUC2 - cup-shaped cotyledon), which is the largest plant transcription factor family, plays an important role in development and stress responses in plants. Protein of this family is characterized by a highly conserved DNA binding domain, known as NAC domain in the N-terminal region. In contrast, the C-terminal region of NAC proteins, usually containing the transcriptional activation domain, is highly diversified both in length and sequence. More than 100 members of this family have been identified in rice. However, only a few of them have been functionally characterized, especially in rice. Gene encoding transcription factor OsNAC1 has been proved to play an important role in drought stress in plants. The CaMV35S promoter derived from the common plant virus, cauliflower mosaic virus (CaMV), is a component of transgenic constructs in more than 80% of genetically modified (GM) plants. It is the promoter of choice for plant genetic engineering, as it is a strong and constitutive promoter. In this study, an expression vector harboring OsNAC1 in the form of CaMV35S:OsNAC1:Nos was constructed and transferred into J02 (Oryza sativa L. Japonica) rice plants via Agrobacterium tumefaciens. The presence of the transgene was confirmed by PCR using OsNAC1 specific primers. T0 CaMV35S:OsNAC1:Nos transgenic lines were selected from transgenic plants. The obatained results are expected to be further exploited for development of stress tolerant rice varieties in the future.

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The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

Plants ◽

10.3390/plants8110498 ◽

2019 ◽

Vol 8 (11) ◽

pp. 498

Author(s):

Cuong D. Nguyen ◽

Holden Verdeprado ◽

Demeter Zita ◽

Sachiyo Sanada-Morimura ◽

Masaya Matsumura ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Genetic Background ◽

Oryza Sativa L ◽

Rice Variety ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Japonica Rice ◽

Rice Varieties ◽

Nilaparvata Lugens Stål

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

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Geographic pattern of variation among Asian native rice cultivars (Oryza sativa L.) based on fifteen isozyme loci

Genome ◽

10.1139/g88-126 ◽

1988 ◽

Vol 30 (5) ◽

pp. 782-792 ◽

Cited By ~ 19

Author(s):

J. C. Glaszmann

Keyword(s):

Genetic Diversity ◽

Oryza Sativa ◽

Oryza Sativa L ◽

Indian Subcontinent ◽

Indica Rice ◽

Japonica Rice ◽

Geographic Pattern ◽

Cultivated Rice ◽

Rice Varieties ◽

Himalayan Foothills

The geographic pattern of isozyme variation among rice varieties (Oryza sativa L.) in Asia is described based on an electrophoretic survey of 1688 accessions for 15 loci. The distribution patterns are strongly determined by the existence of several varietal groups that are characterized by contrasting multilocus types with dissimilar environmental and macrogeographic distributions. The two main groups correspond to the indica and japonica subspecies. Other types are frequently found in the Indian subcontinent, especially along the Himalayan foothills. These types are predominant in the Indus River basin. They are differentiated into four groups in the eastern part of the Himalayan foothills. There is variation within the groups. Non-random allele distributions are observed, such as regional clines and narrow localization of alleles. Diversity among indica rice is evenly distributed in whole tropical Asia. Variation among japonica rice shows the hilly part of continental Southeast Asia to be the region of highest genetic diversity and its probable area of origin. All this information provides a guide for further analysis aimed at elucidating the history of cultivated rice in Asia and, subsequently, in other continents.Key words: Asian rice, genetic diversity, isozymes, geographic distributions.

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A large-scale analysis of seed natural aging revealed stronger aging resistance in weedy rice than coexisting cultivated rice for continuous 4 years across China

10.21203/rs.2.17773/v1 ◽

2019 ◽

Author(s):

weimin dai ◽

Yuan Wang ◽

Yu-Jie Zhang ◽

Xi-Xi Sun ◽

Jin-Ling Yang ◽

...

Keyword(s):

Oryza Sativa ◽

Large Scale ◽

Oryza Sativa L ◽

Germination Rate ◽

Weedy Rice ◽

Japonica Rice ◽

Rice Cultivars ◽

Cultivated Rice ◽

Rice Varieties ◽

Aging Resistance

Abstract Increasing resistance to aging is conducive to seed storage and germination rate of crop. Meanwhile, the resistance to aging is one of the important adaptive mechanisms of weed to thrive in farmland. Weedy rice (Oryza sativa f. spontanea) and cultivated rice (Oryza sativa L.) provide a unique pair demonstrating a weed and conspecific model crop that can be used to study the aging resistance of plants across a diverse geographical range. Chinese weedy rice derived from de-domestication of cultivated rice has rapidly risen to malignant weeds, though the hazard has only been reported for about 20 years. Whether weedy rice rapidly evolves higher seed aging resistance than cultivated rice during the process of dedomestication, which is conducive to its persistence in rice fields, is still unclear. In this experiment, the seeds of weed rice populations and their co-existing rice varieties were collected from 61 regions of China and germinated under normal and high temperatures for consecutive four years (2013–2016). Our study found that the aging resistance of weedy rice was higher than that of the co-existing rice cultivars, and weedy rice may have evolved a different aging resistance mechanism than rice cultivars and could be used as a germplasm resource to cultivate aging-resistant rice. The indica-type has strong aging resistance and no dormancy, while the japonica type has weak aging resistance and a little weak dormancy. Thus, by introducing indica-type aging-resistant alleles into japonica rice, cultivation of aging-resistant japonica rice could be possible.

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Genome-wide investigation and expression analysis of APETALA-2 transcription factor subfamily reveals its evolution, expansion and regulatory role in abiotic stress responses in Indica Rice (Oryza sativa L. ssp. indica)

Genomics ◽

10.1016/j.ygeno.2020.10.037 ◽

2020 ◽

Author(s):

Sohaib Ahmed ◽

Muhammad Abdul Rehman Rashid ◽

Syed Adeel Zafar ◽

Muhammad Tehseen Azhar ◽

Muhammad Waqas ◽

...

Keyword(s):

Transcription Factor ◽

Oryza Sativa ◽

Abiotic Stress ◽

Expression Analysis ◽

Stress Responses ◽

Oryza Sativa L ◽

Indica Rice ◽

Regulatory Role ◽

Genome Wide ◽

Apetala 2

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Identification of responsive genes and analysis of genes with bacterial-inducible cis-regulatory elements in the promoter regions in Oryza sativa L.

Acta agriculturae Slovenica ◽

10.14720/aas.2020.116.1.1035 ◽

2020 ◽

Vol 116 (1) ◽

pp. 115

Author(s):

Abbas SAIDI ◽

Zohreh HAJIBARAT ◽

Zahra HAJIBARAT

Keyword(s):

Gene Ontology ◽

Oryza Sativa ◽

Stress Responses ◽

Biotic Stress ◽

Ribosomal Proteins ◽

Oryza Sativa L ◽

Protein Translation ◽

Regulatory Elements ◽

Rice Varieties ◽

Time Point

Bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most critical diseases in rice. In order to study rice responsive genes to bacterial stress, microarray data were retrieved from GEO dataset. To identify the responsive genes to biotic stress (bacteria) bioinformatic tools were employed and the data presented in the forms of heatmap, gene ontology, gene network, and cis-element prediction were used. Almost all responsive genes were down-regulated at around 3 h time point and up-regulated 24 h time point in response to bacterial stress in rice varieties (Oryza sativa subs. japonica ‘IR64’, ‘IRBB5’, ‘IRBB7’ and ‘Y73’). Gene ontology showed that genes are involved in different biological processes including translation and cellular protein metabolic processes. Network analysis showed that genes expressed in response to pathogen infection (Xoo) included protein translation, eukaryotic initiation factors (eIFs), ribosomal proteins, protein ubiquitin, and MAPK genes. The genes expressed in response to bacterial stress can enable plant balance between synthesis and degradation of proteins which in turn allows plants for further growth and development. TATA-box and CAAT box had the highest number of cis elements involved in bacterial stress. These genes can provide novel insights into regulatory mechanisms in biotic stress responses in rice. Identification of bacterial stress response/tolerance genes of rice can assist the molecular breeding of new rice varieties tolerant to bacterial stress.

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