scholarly journals Selecion of carrier material and substrate for biofertilizer by-product containing three halophilic plant growth promoting bacteria (Burkholderia cepacia BL1-10, Bacillus megaterium ST2-9 and Bacillus aquimaris KG6-3)

2018 ◽  
Vol 15 (2) ◽  
pp. 381-392
Author(s):  
Nguyễn Khởi Nghĩa ◽  
Nguyễn Thị Kiều Oanh
Keyword(s):  
Rice Bran ◽  
Coal Ash ◽  
Viable Cell ◽  
Cell Number ◽  
Viable Cell Number ◽  
Bacterial Strains ◽  
Brown Sugar ◽  
Carrier Material ◽  
Plant Growth Promoting ◽  
Growth Promoting

In biofertilizer production, carriers and substrates have an important role in maintaining an efficacy of the commercial biofertilizer by-product. The aim of this study was to select the best carrier material and substrate and as well substrate’s moisture to sustainably store biofertilizer by-product containing three halophilic plant growth promoting bacterial strains. They are Bacillus aquimaris KG6-3 (KG6-1), Burkholderia sp. BL1-10 (BL1-10) and Bacillus megaterium ST2-9 (ST2-9) with a function of non-symbiosic nitrogen fixer, phosphorous solubilizing bacteria and IAA producing bacteria, respectively. All the experiments in this study were conducted under the laboratory conditions. Spent coffee ground and domestic coal ash were used as carrier materials. Besides that, rice bran, banana peel, banana flesh, cocopeat and brown sugar were used as substrate materials. The results showed that the domestic coal ash was able to sustain the highest viable cell number of ST2-9 and KG6-3 strains after 16 incubation hours whilst BL1-10 was found to be highest viable cell number in carrier material of spent coffee ground and the viable cell number of mixed inoculum including three bacterial strains was shown to be not significantly different among the three tested carrier materials. Moreover, the viable cell number of all three bacterial strains regardless of single or mixed inoculation was found to be highest during 15 weeks in rice bran substrate with 50% of moisture content. The mixed viable cell number of bacterial consortium achieved highest in the substrate containing rice bran + brown sugar (15:1, w/w) and when taking into account a comparion between two inoculation means, it was shown that the viable cell number in treatments with bacteria immobilized in domestic coal ash was always higher than that in treatments with free cell bacteria inoculation method. Thus, it was concluded that domestic coal ash and rice bran + brown sugar mixture (15:1) was the best carrier material type and substrate for biofertilizer by-product containing the three holophilic plant growth promoting bacteria and the immobilization technique to inoculate bacteria via carrier material was the best option for microbial inoculation.

scholarly journals Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate

2017 ◽  
Vol 71 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Tanja Krunic ◽  
Natasa Obradovic ◽  
Maja Bulatovic ◽  
Maja Vukasinovic-Sekulic ◽  
Kata Trifkovic ◽  
...  
Keyword(s):  
Ph Value ◽  
Starter Culture ◽  
Titratable Acidity ◽  
Viable Cell ◽  
Cell Number ◽  
Alginate Beads ◽  
Viable Cell Number ◽  
Carrier Material ◽  
Fermentative Activity ◽  
Antioxidant Characteristics

The main objectives of this paper were to study the influence of the carrier material used for encapsulation and of bead size to fermentative activity and viability of the dairy starter culture ?Lactoferm ABY 6?. Encapsulation of yoghurt culture in beads with diameter of 1mm provides better results than encapsulation in beads with larger diameter. Alginate beads and chitosan coated beads have proved to be a strong barrier for nutrients from substrate, so samples with those beads have lower viable cell count, lower titratable acidity and higher pH value after 5h of fermentation at 42?C, than samples with WPC-alginate beads. Also those beads have significantly (P < 0.05) lower cell leaking, than WPC-alginate beads and lower antioxidant capacity. Encapsulation of yoghurt culture in WPC-alginate carrier with diameter of approximately 1mm provided the best characteristics for fermented product. Samples with these beads have significantly (P < 0.05) higher increase of viable cell number after fermentation, despite of major cell leaking (19.7 %). Moreover, sample with these beads have the highest titratable acidity, the lowest pH value after fermentation (the best fermentative activity) and the best antioxidant characteristics.

scholarly journals Application of a roller conveyor type plasma disinfection device with fungus-contaminated citrus fruits

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akikazu Sakudo ◽  
Yoshihito Yagyu
Keyword(s):  
Treatment Time ◽  
Citrus Fruit ◽  
Viable Cell ◽  
Cell Number ◽  
Viable Cell Number ◽  
Citrus Fruits ◽  
Decimal Reduction Time ◽  
Gas Plasma ◽  
Plasma Device ◽  
Time Required

AbstractEfficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressure dielectric barrier discharge (APDBP) plasma on a set of rollers. Penicillium venetum was spotted onto the surface of the fruit or pericarps, as well as an aluminium plate to act as a control, before performing the plasma treatment. The results showed that viable cell number of P. venetum decreased with a decimal reduction time (D value or estimated treatment time required to reduce viable cell number by 90%) of 0.967 min on the aluminium plate, 2.90 min and 1.88 min on the pericarps of ‘Kiyomi’ (Citrus unshiu × C. sinensis) and ‘Kawano-natsudaidai’ (C. natsudaidai) respectively, and 2.42 min on the surface of ‘Unshu-mikan’ (C. unshiu). These findings confirmed a fungicidal effect of the plasma not only on an abiotic surface (aluminium plate) but also on a biotic surface (citrus fruit). Further development of the instrument by combining sorting systems with the plasma device promises an efficient means of disinfecting citrus fruits during food processing.

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize

2015 ◽  
Vol 42 (8) ◽  
pp. 770 ◽  
Author(s):  
Saqib Saleem Akhtar ◽  
Mathias Neumann Andersen ◽  
Muhammad Naveed ◽  
Zahir Ahmad Zahir ◽  
Fulai Liu
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Crop Production ◽  
Interactive Effect ◽  
Nutrient Balance ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Salt Affected Soils

The objective of this work was to study the interactive effect of biochar and plant growth-promoting endophytic bacteria containing 1-aminocyclopropane-1-carboxylate deaminase and exopolysaccharide activity on mitigating salinity stress in maize (Zea mays L.). The plants were grown in a greenhouse under controlled conditions, and were subjected to separate or combined treatments of biochar (0% and 5%, w/w) and two endophytic bacterial strains (Burkholderia phytofirmans (PsJN) and Enterobacter sp. (FD17)) and salinity stress. The results indicated that salinity significantly decreased the growth of maize, whereas both biochar and inoculation mitigated the negative effects of salinity on maize performance either by decreasing the xylem Na+ concentration ([Na+]xylem) uptake or by maintaining nutrient balance within the plant, especially when the two treatments were applied in combination. Moreover, in biochar-amended saline soil, strain FD17 performed significantly better than did PsJN in reducing [Na+]xylem. Our results suggested that inoculation of plants with endophytic baterial strains along with biochar amendment could be an effective approach for sustaining crop production in salt-affected soils.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

scholarly journals INVESTIGATION ON QUALITY PROPERTIES OF TRADITIONAL BULK BREAD COVERED WITH PROBIOTICS AND SOYBEAN OIL EDIBLE COATING

2020 ◽  
Vol 49 (2) ◽  
pp. 144-153
Author(s):  
R. Amiri Qandashtant ◽  
E. Ataye Salehi ◽  
A. Mohamadi Sani ◽  
M. Mehraban Sangatash ◽  
O. Safari
Keyword(s):  
Soybean Oil ◽  
Whey Protein ◽  
Corn Starch ◽  
Lactobacillus Rhamnosus ◽  
Methyl Cellulose ◽  
Viable Cell ◽  
Cell Number ◽  
Whey Protein Concentrate ◽  
Viable Cell Number ◽  
Quality Properties

Probiotic food products are available at the supermarket commercially, but probiotic bakery products are much less in evidence. In the present study, methyl cellulose (2%), whey protein concentrate (2%), corn starch (1%), and soybean oil at 2, 4, and 6% were used for coating layer on the bulked bread surface, and then the quality properties were studied. The results showed that Lactobacillus rhamnosus GG, as probiotic component of the coating, immobilized in corn starch, whey protein, and methyl cellulose films had enhanced viability throughout shelf-life. The probiotics remained viable for 4 days, maintaining high viable cell number levels. Adding soybean oil at 6% concentration enhanced texture, sensory properties, and image index during storage.

scholarly journals Classification of Five Uremic Solutes according to Their Effects on Renal Tubular Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Takeo Edamatsu ◽  
Ayako Fujieda ◽  
Atsuko Ezawa ◽  
Yoshiharu Itoh
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Viable Cell ◽  
Cell Number ◽  
Viable Cell Number ◽  
Cycle Progression ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Uremic Solutes ◽  
Renal Tubular

Background/Aims. Uremic solutes, which are known to be retained in patients with chronic kidney disease, are considered to have deleterious effects on disease progression. Among these uremic solutes, indoxyl sulfate (IS) has been extensively studied, while other solutes have been studied less to state. We conducted a comparative study to examine the similarities and differences between IS,p-cresyl sulfate (PCS), phenyl sulfate (PhS), hippuric acid (HA), and indoleacetic acid (IAA).Methods. We used LLC-PK1 cells to evaluate the effects of these solutes on viable cell number, cell cycle progression, and cell death.Results. All the solutes reduced viable cell number after 48-hour incubation. N-Acetyl-L-cysteine inhibited this effect induced by all solutes except HA. At the concentration that reduced the cell number to almost 50% of vehicle control, IAA induced apoptosis but not cell cycle delay, whereas other solutes induced delay in cell cycle progression with marginal impact on apoptosis. Phosphorylation of p53 and Chk1 and expression of ATF4 and CHOP genes were detected in IS-, PCS-, and PhS-treated cells, but not in IAA-treated cells.Conclusions. Taken together, the adverse effects of PCS and PhS on renal tubular cells are similar to those of IS, while those of HA and IAA differ.

Sign in / Sign up

Export Citation Format

Share Document