scholarly journals FRUIT SEEDS, SEEDS OF GRAPE AND CUCURBITACEOUS – A PROSPECTIVE SOURCE OF OBTAINING BIOLOGICALLY ACTIVE SUBSTANCES. OVERVIEW

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
K. Gafurov ◽  
B. Muslimov

Republic of Uzbekistan is a major producer of agricultural products. The production of vegetables, grapes, fruits and berries, melons and gourds are increasing every year. The total volume of their production is approximately up to 16200 thousand tons per year. The seeds of these crops make up from 2 to 10% of the mass of processed raw materials, which are sources of valuable biologically active substances. The main method of obtaining oil (from apricot kernel, grape and melon seeds) is cold pressing, i.e. pressing carried out at a temperature below 60°C. Such oil immediately goes from the category of ordinary to the category of medicinal. At present, in Uzbekistan, the processing of apricot kernels, grape and melons seeds with the aim of to obtain high-quality vegetable oil is carried out on low-tonnage technological lines by the method of cold pressing. However, this method of processing raw materials does not provide the maximum yield of valuable vegetable oil and nutrients. Residual oilcake is not processed to obtain biologically active substances. The use of extraction as a method of obtaining these substances is not practiced. Unlike other solvents such as butane, hexane has the advantage of being virtually harmless to the user in ethanol extraction. Unlike the so-called "CO2-extraction", the extraction with ethanol can be carried out without the use of expensive equipment and subject to the correct technological regulations; the obtained extract can be of a very high-quality spectrum.  A scientifically substantiated methodology for the engineering calculation of a plant for the extraction of ingredients from plant materials with a low capacity, operating on an ethanol solvent, has not been developed, which shows the relevance of the development and implementation of such equipment in production.

Author(s):  
Natalya Kostina ◽  
Tatiana Podlegaeva ◽  
Irina Sergeeva

Introduction. Violation of the ecological balance and modern processing methods may result in a lower content of important nutrients in food. Therefore, there is a need to restore the balance of biologically active substances in food products. In this regard, it seems appropriate to use plant materials that contain biologically active substances, as well as pigments. The use of aromatic plants for these purposes remains understudied. The research objective was to extract chlorophyll-based green pigments based from green plants. Study objects and methods. The object of the study was dried parsley (Petroselinum vulgare). The extractants included acetone, ethyl alcohol (95%), hexane, petroleum ether, and chloroform. Results and discussion. The extractants were chosen according to the total content of chlorophylls, which determine the color of the extract. Since carotenoids are extracted along with chlorophylls, the content of this component was also determined. Acetone demonstrated the maximum degree of chlorophyll recovery. However, this solvent cannot be used in food industry. Therefore, 95% ethanol, which also showed a high degree of chlorophyll extraction, was chosen as the extractant. A set of experiments made it possible to establish the ratio of feedstock and extractant as 1:60 (three times extraction with a mass ratio of parsley and extract); the optimal time of each extraction was 0.5 ± 0.1 h. Magnesium carbonate (MgCO2) helped to preserve the dyes. In order to increase the concentration of coloring substances, the obtained extract was subjected to thickening at a residual pressure of 400 Pa at 40°C. Conclusion. The obtained concentrate from dried parsley can be used in production of combined foods.


2021 ◽  
Vol 17 (2) ◽  
pp. 56-66
Author(s):  
A. A. Fabritskaya ◽  
S. O. Semenikhin ◽  
V. O. Gorodetsky ◽  
N. I. Kotlyarevskaya ◽  
E. P. Victorova

The article overviews modern studies of domestic and foreign scientists in the field of extraction of biologically active substances from plant materials using enzymes. Extraction of biologically active substances using enzymes allows for controlled biotransformation of plant materials due to the precise specificity and selectivity of enzymes, which creates mild conditions that ensure the preservation of biologically active substances. It has been noted that the quality of the extractant used has a significant effect on the efficiency of the extraction process. Thus, raw materials treated with a combination of an electrolyte and an enzyme are extracted more efficiently by reducing the mass transfer barrier. The main advantage of using supercritical fluids as an extractant is a decrease in temperature or pressure that leads to the precipitation of the extractable substance. One of the main disadvantages of the process of extracting biologically active substances from plant materials using enzymes is their high cost. To eliminate this disadvantage, the following intensification methods are used: ultrasonic treatment, high pressure treatment and microwave treat- ment. The most promising methods for intensifying the process of extracting biologically active substances from plant materials using enzymes are methods of ultrasonic and microwave treatment. Ultrasonic treatment under optimal conditions allows increasing the activity of enzymes, and microwave treatment provides more efficient penetration of the extractant into the tissues of plant material, due to the destruction of cell walls.


2019 ◽  
Vol 8 (3) ◽  
pp. 35-39 ◽  
Author(s):  
S. S. Belokurov ◽  
E. V. Flysyuk ◽  
I. E. Smekhova

Introduction. According to the literature, modern technologies, in particular, extraction in an ultrasonic field, make it possible to obtain concentrates of biologically active substances (BAS) with almost complete preservation of the chemical composition inherent in natural raw materials and high yield of extractive substances. At the same time, the ability to regulate the concentration of recoverable active substances during the technological process opens up prospects for the use of natural components as the main pharmaceutical substance.Aim. The purpose of this study was to select the optimal extraction method for obtaining extracts with a high content of BAS from fenugreek seeds. Materials and methods. Extracts were obtained at a temperature of 60±2 °С, by the method of dynamic maceration using an ultrasonic device. Ultrasonic extraction was performed using an ultrasonic installation I100-6/4. In both cases, purified water and ethanol solutions in various concentrations from 40 to 90% were used as extractants. The ratio of raw materials: extractant was as 1:10 (by weight). A portion of vegetable raw materials (10 g) was placed in a glass and poured 100 cm3 of extractant. Next, maceration or sonication was performed. Maceration was performed with magnetic stirring, the rotation speed was 100 rpm. Extraction was carried out at a temperature of 55–60 °C for 5 hours. Ultrasonic effects on solid plant materials were carried out with an intensity in the range from 17 to 22 kHz for 30–60 minutes.Results and discussion. Studies have allowed to determine the amount of extractives in the seeds of fenugreek hay, and choose the most promising method of extraction to obtain extracts with a high content of biologically active substances. A comparison was made between dynamic maceration and ultrasonic extraction. The most promising was the method of ultrasonic extraction at an oscillation frequency of 22 kHz. For 1 hour of extraction, it was possible to achieve depletion of raw materials. It is established that the content of extractable substances directly proportional depends on the duration of treatment and the frequency of ultrasonic waves. The correlation coefficient was 0.78.Conclusion. Ultrasonic extraction when exposed for 60 minutes allows you to get a greater amount of extractives compared with dynamic maceration. To achieve the same level of extractive substances by the method of dynamic maceration, the raw materials must be processed within 5 hours.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 518d-518
Author(s):  
W. Letchamo ◽  
V.S. Krutilina

Echinace from the Asteracea family has been among the top 10 marketed herbs in North America and Europe. So far, only a limited amount of Echinace originate from commercial cultivation. Echinacea purpurea has been commercially cultivated in the United States since the mid 1970s, in Russia since 1935, while in Europe since the 1960s. However, there has been no published information in the English language on methods of cultivation, expected yields, limiting factors, nutrient requirements, and the optimum plant development stage to obtain maximum yield and concentration of the biologically active substances for pharmaceutical and cosmetic processing. The objective of this investigation was, therefore, to study the general distribution of major nutrients and biologically active substances and the relationship between the two groups, and the pattern of the accumulation of cichoric acid, echnacoside, isobutylamides (tetraenes) and essential oil in commercially cultivated Echinacea purpurea. There was a significant variation in the content of essential oil, cichoric acid, and isobutylamides (tetraenes) in different organs and stages of the plant development. Similarly, the difference in the distribution of N, P, K, Ca, Mg, and Fe in different organs and developmental stages is demonstrated. The correlation between the content of the active substances and nutrients is demonstrated, while recommendations for an optimum harvesting stage and date are made.


Author(s):  
SHAPOVALOVA Nataliia ◽  
VEZHLIVTSEVA Svitlana ◽  
ANTIUSHKO Dmytro

Background. The deficiency of essential nutrients in the human body is the root cause of reduced efficiency, general resistance of the body to various diseases. That is an urgent problem in the context of the spread of coronavirus infection. In this regard, the search for opportunities to meet the needs of the population in food products that contain biologically active substances (BAS) through the use of high-value and at the same time available plant raw materials is extremely important. Marigolds attract special attention among such raw materials. The aim of the work is to study the chemical composition and evaluate the consumer properties of Tagetes L. varieties, zoned in the Kiev region, to prove the possibility of increasing the biological value of pasta by adding to their recipe powder from marigold inflorescences. Materials and methods. The object of research is pasta of B group (noodles). Inflorescences of marigolds of different varieties gathered during the flowering period in July-September 2019–2020 in the territory of Vasylkiv and Obukhiv districts of Kyiv region, Ukraine were used as enriching plant raw materials. The quality of finished pasta was assessed by organoleptic and physicochemical indexes (moisture content and titratable acidity). The content of mineral elements in the fine powder of marigold inflorescences was determined on the EXHERT-3L device, the quantitative composition and BAS identification – by spectrophotometric analysis on the device Specord-200 Analytic Jena UV-vis. The integrated quality index of pasta products was calculated taking into account the importance factors of individual indexes. Results. Comparing the obtained results, we can state that all the studied varieties – Gold Kopfen, Orange Flame, Hawaii, Equinox are characterized by high biological value, regardless of the area of collection. As a result of calculated and experimental studies it was determined that the addition of 7 % of the supplement does not provide the required supply of vitamins and minerals. Replacing the flour in the recipe by 20 % significantly improves the mineral composition, but deteriorates the taste and aromatic properties of pasta. The most optimal and rational amount of added additive is 15 %, as it improves the organoleptic properties of pasta and enriches their BAS. Conclusion. The usage of non-traditional vegetable raw materials, in particular powder from marigold inflorescences, which is rich in biologically active substances, allows not only to expand the range, improve the quality of finished products, but also increase the biological value of the product. The optimal amount of additive –powder from marigold inflorescences, was defined. It is 15 % by weight of flour in the production of noodles. This concentration allows not only to improve the organoleptic properties, but also to increase the content of biologically active substances – vitamins, micro- and macronutrients. The usage of non-traditional natural raw materials (powder from marigold inflorescences) allows to abandon artificial colorsand give the finished pasta a pleasant colorand aroma.


2018 ◽  
pp. 30-36
Author(s):  
V. M. Minarchenko ◽  
A. Yu. Butko

The popularity of herbal remedies and medicines, with the participation of biologically active substances from plants is traditionally high. The annual increase of the domestic market for herbal remedies is due to the preservation and modernization of production capacities for processing of medicinal vegetative raw materials in Ukraine, introduction of modern scientific developments, a large natural resource base of medicinal plants and attracting investments in the development of this industry. The aim of our work was the study of the domestic market of medicines of plant origin, which includes herbal remedies, substances and preparations with the participation of biologically active substances from plants. The object of the study was domestic medicines of plant origin that are registered in Ukraine for 2012–2017. The study used the analytical, statistical methods, and the results of its own resource studies of medicinal plants. Phytopharmaceutical market of Ukraine is actively developing due to the attraction of new types of medicinal plants and expanding the range of medicines. The number of domestic drugs with the participation of biologically active substances from plants has increased by 17.4% compared with 2014. It is established that as of 1 January 2017 in Ukraine was 1 141 the form of medicinal drugs of plant origin. Of them: 645 – domestic products, 496 – foreign. Most of the herbal drugs in the pharmaceutical market of Ukraine are made from wild and cultivated domestic raw materials.


2020 ◽  
Vol 100 (4) ◽  
pp. 52-59
Author(s):  
А.S. Kishkentayeva ◽  
◽  
S.N. Mantler ◽  
М.М. Zhakanov ◽  
S.М. Adekenov ◽  
...  

The review summarizes data on biologically active compounds of Achillea nobilis L. and methods of their isolation. From Achillea nobilis L., collected in different places of growth, the following have been isolated: essential oil, the main components of which are monoterpene compounds; sesquiterpene lactones estafiatin, hanphyllin, anobin, chrysartemine A, canin, anolide and tanapartin-β-peroxide; the steroid acetyleucanbin; flavonoids: 3,5-dihydroxy-6,7,8-trimethoxyflavone, 5-hydroxy-3,6,7,4’-tetramethoxyflavone and 5,3’-di-hydroxy-3,6,7,4’-tetramethoxyflavone. It has been determined that the component composition of the essen-tial oil of Achillea nobilis L. largely depends on the soil and climatic factors in the places of its growth, the phase of the growing season and the method of its extraction from plant raw materials, and the extractant used (chloroform, ethanol, hot water, diethyl ether). Antibacterial, antimicrobial, antioxidant, antiparasitic activi-ties are characteristic both for the sums of extractive substances from Achillea nobilis L. and for individual compounds isolated from them. Methods for the isolation of biologically active substances from Achillea nobilis L. for the development of new drug substances are described. The main aim of this work was a com-parative analysis of the available research results on the phytochemical study of Achillea nobilis L.


Author(s):  
G.А. Zhorov ◽  
◽  
L.L. Zakharova ◽  
V.N. Obryvin ◽  
◽  
...  

For correctly assess the effectiveness and safety of the use of sorption-detoxifying agents and feed additives intended for animals receiving feed with an excess content of toxicants, it is necessary to take into account the background levels of radionuclides, toxic elements, pesticides and other technogenic and natural pollutants in the studied substances. The need for such studies is due both to the existence of areas with a naturally elevated level of natural toxicants in the sources of raw materials for the production of additives and drugs, and to the increasing anthropogenic influence, accompanied by the entry of xenobiotics into environmental objects. In series of radiometric studies, the specific activity of technogenic and natural radioactive isotopes (90Sr, 137Cs, 40К, 226Ra, 232Th) in a number of sorption-detoxifying and biologically active substances and preparations used in animal husbandry and veterinary medicine as part of feed additives and pharmacological agents was determined. It was found that in 43% of the studied samples, the level of specific activity of 1,1Sr in 3-90 times higher than allowed by the current standards. The maximum level of 90Sr, equal to (137±9) Bq/kg, was detected in perlite. The specific activity of 137Cs did not exceed the permissible level: in mineral sorbents its amount reached (40±7) Bq/kg, in organic and complex sorbents – (24±4), and in feed additives – (29±8) Bq/kg. The maximum levels of 40K were (1429±83) Bq/ kg in minerals (radionite) and (2613±100) Bq/kg in organic substances (lignohumate). The levels of 226Ra and 232Th did not exceed (153±13) and (79±13) Bq/kg, respectively, and were higher in the mineral samples.


Author(s):  
Е.П. ВИКТОРОВА ◽  
А.Д. АЧМИЗ ◽  
М.В. ЛУКЬЯНЕНКО ◽  
С.О. СЕМЕНИХИН

При производстве качественной пищевой продукции особое внимание необходимо уделять ее упаковыванию и хранению. Витаминно-минеральные концентраты, используемые для приготовления пищевых продуктов и получения качественной безопасной продукции животноводства, содержат комплекс лабильных биологически активных веществ, для сохранности которых необходимо минимизировать окислительные процессы. Это обусловливает повышенные требования к упаковке таких продуктов. С целью выбора упаковочного материала и способа упаковки витаминно-минерального концентрата проведен тематический обзор публикаций отечественных и зарубежных ученых. Рассмотрены тенденции в области применения упаковочных материалов, указаны преимущества и недостатки биополимеров. На основе проведенного анализа установлено, что для упаковывания витаминно-минерального концентрата целесообразно в качестве материала упаковки использовать полимерные пленки, обладающие высокой свето- и газонепроницаемостью, позволяющей снизить скорость протекания окислительных процессов и, следовательно, сократить потери содержащихся в продукте лабильных биологически активных веществ. В качестве способа упаковки можно рекомендовать применять вакуумирование, обеспечивающее отсутствие кислорода воздуха в упаковке. When producing high-quality food products, special attention should be paid to their packaging and storage. Vitamin and mineral concentrates used for food preparation and production of high-quality safe livestock products contain a complex of labile biologically active substances, for the safety of which it is necessary to minimize oxidative processes. This leads to increased requirements for the packaging of such products. In order to select the material and method of packaging of vitamin and mineral concentrates, a thematic review of publications of domestic and foreign scientists was conducted. Trends in the use of packaging materials are considered, advantages and disadvantages of biopolymers are indicated. It was found that for packaging vitamin and mineral concentrate, polymer pellicle with high light and gas tightness, which allows reducing the rate of oxidative processes and, consequently, reducing the loss of labile biologically active substances contained in the product, should be used as a packaging material. Vacuuming, which ensures the absence of oxygen in the air in the package, can be recommended as a method of packaging.


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