scholarly journals Аcinetobacter baumannii bv Tryptophandestruens bv nov. isolated from clinical samples

2021 ◽  
Vol 11 (5) ◽  
pp. 965-972
Author(s):  
E. P. Sivolodskii ◽  
L. A. Kraeva ◽  
D. A. Starkova ◽  
N. V. Mikhailov ◽  
G. V. Gorelova
Keyword(s):  
Reference Strain ◽  
Bacterial Species ◽  
Synthetic Medium ◽  
Taxonomic Status ◽  
Acinetobacter Calcoaceticus ◽  
Rpob Gene ◽  
Clinical Samples ◽  
Military Medical Academy ◽  
The Military ◽  
Atypical Strains

The aim of the study was to determine the taxonomic status of a group consisting of atypical strains of Acinetobacter baumannii, outline relevant characteristics and methods necessary for their identification. There were examined 10 strains of A. baumannii (6 of them primary comprised) bearing similar profile of atypical features isolated from clinical samples (urine, sputum) in 2017–2019 at the Military Medical Academy. Сlinical strains of typical A. baumannii (n = 36), Acinetobacter nosocomialis (n = 14), Acinetobacter pittii (n = 9) and 1 strain of Acinetobacter calcoaceticus isolated from the external environment were used in comparative studies. Atypical strains had the characteristics of A. calcoaceticus — A. baumannii (ACB) complex bacteria and were identified as A. baumannii. The utilization of substrates as the only carbon source was studied on a dense synthetic medium added with 0.2 % substrate during incubation for 72 hours at 37°C. Carbohydrate oxidation coupled to acid formation was detected on the Hugh–Leifson medium by using a micromethod. Aromatic amino acid biotransformation was carried out in liquid and dense nutrient media assessed in chromogenic reaction. The rpoB gene was used for strain genetic characterization. Amplification of two 940 and 1210 base pair (bp)-long fragments from the rpoB gene was performed by the routine polymerase chain reaction using primers with previously described sequences. Amplification products were sequenced by Sanger using Big Dye Terminator v3.1 (Applied Biosystems, USA) and capillary electrophoresis on an automatic sequencer ABI PRISM 3130 (Applied Biosystems, USA), followed by using methods for determining the similarity levels of sequenced fragments with the rpoB gene sequences of the reference strain A. baumannii ATCC 17978 (GenBank accession no. CP053098.1). It was found that all strains belonging to atypical A. baumannii spp. had a specific set of features that distinguish them from typical strains of A. baumannii as well as other types of the ACB complex: detected biotransformation of L-tryptophan (via anthranilate pathway) and anthranilic acid under unambiguous lack of such signs in other bacteria; lack of utilized sodium hippurate and L-arabinose being unambiguously evident in other bacteria; lack of utilized L-tryptophan, putrescine, L-ornithine being utilized in the majority of strains of belonging to other bacterial species. Genetic analysis showed that the control strains of typical A. baumannii displayed 99.20–99.21% similarity within the sequenced fragments of the rpoB gene with those from the rpoB gene of the reference strain. All 10 strains of atypical A. baumannii had similar features (99.20–99.21%). At the same time, parameters of control strains from other bacterial species significantly differed: A. nosocomialis (95.10–95.97%), A. pittii (94.63–94.92%), A. calcoaceticus (93.00%). Hence, the strains of atypical and typical A. baumannii are genetically homogeneous and belong to the same species. The data presented allow us to consider this group of atypical A. baumannii strains as a new biovar. We propose the name for this new biovar — tryptophandestruens (tryptophan-destroying) stemming from the Latin word destruens — destroying. Identification of A. baumannii bv. tryptophandestruens bacteria can be carried out in laboratory of any level by using tests for L-tryptophan biotransformation as well as sodium hippurate utilization.

scholarly journals Rapid identification of health care–associated infections with an integrated fluorescence anisotropy system

2016 ◽  
Vol 2 (5) ◽  
pp. e1600300 ◽  
Author(s):  
Ki Soo Park ◽  
Chen-Han Huang ◽  
Kyungheon Lee ◽  
Yeong-Eun Yoo ◽  
Cesar M. Castro ◽  
...  
Keyword(s):  
Health Care ◽  
Fluorescence Anisotropy ◽  
Bacterial Species ◽  
Rapid Identification ◽  
Integrated System ◽  
Clinical Samples ◽  
Drug Resistant ◽  
Polarization Anisotropy ◽  
Major Health ◽  
Health Care Associated Infections

Health care–associated infections (HAIs) and drug-resistant pathogens have become a major health care issue with millions of reported cases every year. Advanced diagnostics would allow clinicians to more quickly determine the most effective treatment, reduce the nonspecific use of broad-spectrum antimicrobials, and facilitate enrollment in new antibiotic treatments. We present a new integrated system, polarization anisotropy diagnostics (PAD), for rapid detection of HAI pathogens. The PAD uses changes of fluorescence anisotropy when detection probes recognize target bacterial nucleic acids. The technology is inherently robust against environmental noise and economically scalable for parallel measurements. The assay is fast (2 hours) and performed on-site in a single-tube format. When applied to clinical samples obtained from interventional procedures, the PAD determined the overall bacterial burden, differentiated HAI bacterial species, and identified drug resistance and virulence status. The PAD system holds promise as a powerful tool for near-patient, rapid HAI testing.

scholarly journals SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades

2021 ◽  
Vol 7 (6) ◽  
pp. 433
Author(s):  
Ahmad Ibrahim ◽  
Lucie Peyclit ◽  
Rim Abdallah ◽  
Saber Khelaifia ◽  
Amanda Chamieh ◽  
...  
Keyword(s):  
Culture Medium ◽  
Limit Of Detection ◽  
Bacterial Species ◽  
Multidrug Resistant ◽  
Rapid Identification ◽  
Clinical Samples ◽  
Candida Auris ◽  
Phenotypic Profile ◽  
Stool Samples ◽  
Selective Culture Medium

Candida auris is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium for all C. auris clades. This medium is sensitive with a limit of detection ranging between 101 and 102 CFU/mL. The 100% specificity of SCA (specific C. auris) medium is confirmed on a set of 135 Candida strains, 50 bacterial species and 200 human stool samples. Thus, this medium specifically selects for C. auris isolation from clinical samples, allowing the latter to study its phenotypic profile.

Comparative analysis of the economic costs of treating patients with non-infectious diseases of internal osteosynthesis

2020 ◽  
Vol 22 (4) ◽  
pp. 156-160
Author(s):  
V. V. Khominets ◽  
V. Yu. Tegza ◽  
I. V. Foos ◽  
E. M. Pugaev
Keyword(s):  
Comparative Analysis ◽  
Indirect Costs ◽  
Infectious Complications ◽  
Long Bones ◽  
Economic Costs ◽  
Direct And Indirect Costs ◽  
Military Medical Academy ◽  
Limb Fractures ◽  
Primary Osteosynthesis ◽  
The Military

A comparative analysis of the economic costs of revision surgical treatment of 121 patients with non-infectious complications of internal osteosynthesis and 59 patients who underwent primary osteosynthesis for fractures of the long bones of the limbs with an uncomplicated course was carried out. General economic costs include direct and indirect costs. It was found that the total costs for the treatment of patients suffering from non-infectious complications of internal osteosynthesis of the long bones of the extremities, who are in the clinic of military traumatology and orthopedics. G.I. Turner of the Military Medical Academy in the period from 2007 to 2018 revision surgery was performed, amounted to 279637238 rubles, which corresponds to 2311051,55 rubles per patient. At the same time, the total costs for the treatment of patients with fractures of the long bones of the extremities without complications amounted to 44960873 rubles (762048,69 rubles per patient). The total cost per patient with a non-infectious complication of internal osteosynthesis of the femur was 2540499,54 rubles (483824,85 straight, 2056674,69 indirect), of the leg bones 2333762,17 rubles (474501,47 straight, 1859260,70 indirect), the humerus 1830362,75 rubles (399942 straight, 1430420,75 indirect), the bones of the forearm 1804664,6 rubles (339700 straight, 1464964,6 indirect). It was revealed that due to failures after primary osteosynthesis of limb fractures and complications, the costs associated with the direct and indirect costs of their treatment increase. In this regard, further research is needed to improve the treatment of complications of internal osteosynthesis and to develop a set of measures for their prevention. This is important both for the patient and for the health care system as a whole.

Anniversary of Professor V.M. Aristovsky

1935 ◽  
Vol 31 (1) ◽  
pp. 150-151
Author(s):  
V. Gruzdev
Keyword(s):  
25Th Anniversary ◽  
Military Medical Academy ◽  
Medical Academy ◽  
Red Army ◽  
The Military

On 23 November 1934, the Military Medical Academy of the Red Army solemnly honoured one of its most prominent professors, Vyacheslav Mikhailovich Aristovsky, head of the Department of Microbiology, on the occasion of his 25th anniversary of medical, social and scientific and pedagogical activities.

scholarly journals Microvascular osteoseptocutaneous radial flap in reconstruction of mandible following war injury

2005 ◽  
Vol 62 (6) ◽  
pp. 429-434 ◽  
Author(s):  
Srboljub Stosic ◽  
Jefta Kozarski ◽  
Tatjana Stosic-Opincal ◽  
Nebojsa Jovic ◽  
Ruzica Kozomara
Keyword(s):  
Maxillofacial Surgery ◽  
Late Complications ◽  
War Injuries ◽  
Military Medical Academy ◽  
Medical Academy ◽  
The Face ◽  
Compound Graft ◽  
The Military ◽  
Outstanding Quality

Background. Vascularized osteoseptocutaneous radial flap is commonly used in the reconstruction of composite bony and soft tissue defects of the lower third of the face due to the outstanding quality of its cutaneous component. The aim was to evaluate the primary and overall success in the reconstruction of mandibular defects, following war injuries, with vascularized osteoseptocutaneous radial flap. Methods. At the Department of Maxillofacial Surgery of the Military Medical Academy Belgrade, there were eight patients with this kind of defect following war injury, and the mandible was reconstructed with a vascularized osteoseptocutaneous radial flap. Bony compartment of the graft was harvested as up to 11 cm long segment of radial circumference. Results. The localization and structure of the defect, features of a harvested compound graft, the procedure of the reestablishment of the mandibular continuity was presented as well as immediate and late complications during the consolidation period, and the primary successful reconstruction in 87.5% of the patients. Conclusion. The primary and overall success in the mandibular defects reconstruction with a vascularized osteoseptocutaneous radial flap was equal or even better than those presented in the literature on the reconstruction of the similar defects after tumor resections.

scholarly journals The General, His Fandom, and a Participatory Pandemic

2021 ◽  
Vol 82 ◽  
pp. 53-78
Author(s):  
Angelina Ilieva ◽  
Keyword(s):  
Focal Point ◽  
Public Image ◽  
Fan Culture ◽  
The Public ◽  
Military Medical Academy ◽  
Medical Academy ◽  
The Media ◽  
Characteristic Features ◽  
The Military

In February 2020, the Bulgarian government established the National Operational Headquarters for Combating the COVID-19 Pandemic in Bulgaria. General Ventsislav Mutafchiyski, a military doctor, professor at the Military Medical Academy in Sofia, was appointed as its chairman. This paper presents a case study on the public image of Ventsislav Mutafchiyski, its readings and interpretations by the audience, and the specific fan culture that emerged around his media persona during the first wave of the COVID-19 pandemic in Bulgaria. Placed in the spotlight of the media at the very beginning of the crisis, Mutafchiyski became extremely popular as the public figure most strongly associated with the fight against the spread of the disease in the country. Around his media persona, shaped in the public imagination as a wartime leader, a fan culture has grown with all its characteristic features and dimensions: fans and anti-fans, affirmative and transformative fandom. As a fictional character, Mutafchiyski has appeared in numerous forms of vernacular creativity: poems, songs, material objects, jokes, fake news, conspiracy theories, and memes. In this way, the General has become the main character of Bulgarian pandemic folklore and the focal point of a participatory pandemic.

scholarly journals The first outbreak of brucellosis in the region of Sabac

2010 ◽  
Vol 67 (8) ◽  
pp. 634-637 ◽  
Author(s):  
Ljiljana Markovic-Denic ◽  
Vesna Skodric-Trifunovic ◽  
Vladimir Zugic ◽  
Dragana Radojcic ◽  
Goran Stevanovic
Keyword(s):  
Confidence Interval ◽  
Animal Species ◽  
Epidemiological Methods ◽  
Human Brucellosis ◽  
Reference Laboratory ◽  
Serological Tests ◽  
Military Medical Academy ◽  
Medical Academy ◽  
Laboratory Confirmation ◽  
The Military

Background/Aim. In Serbia brucellosis is a primary disease of the animals in the southern parts of the country. The aim of this study was to describe the first outbreak of human and animal brucellosis in the region of Sabac, Serbia. Methods. An epidemiological investigation was conducted to identify a source of outbreak and the ways of transmission of brucellosis infection in human population. A descriptive and analytical epidemiological methods (cohort study) were used. Additional data included monthly reports of the infectious diseases from the Institutes of Public Health and data from the Veterinary Specialistic Institute in Sabac. The serological tests for human brucellosis cases were performed in the Laboratory of the Military Medical Academy; laboratory confirmation of animal brucellosis cases was obtained from the reference laboratory of the Faculty of Veterinary Medicine, Belgrade. Results. Twelve cases of brucellosis were recorded from February 9 to September 1, 2004. Total attack rate was 8.1% (7.5% of males, 14.2% of females). Relative risk (RR) of milk consumption was 8.9 (95% confidence interval: 1.63-13.38), and RR for direct contact with animals was 14 (95% confidence interval: 3.5-55.6). The prevalence of seropositive animals in 33 villages of the Macva region accounted for 0.8%. Regarding animal species, sheep were predominant - 264 (95.7%). Out of a total number of seropositive animals, ELISA results were positive in 228 (88.7%) of them. Conclusion. As contact epidemics generally last longer, it is probable that the implemented measures of outbreak control did reduce the length of their duration.

scholarly journals NodC-based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar

2019 ◽  
Vol 70 (1) ◽  
pp. 59-67
Author(s):  
Anna Lenart-Boroń ◽  
Tadeusz Zając ◽  
Piotr Mateusz Boroń ◽  
Agnieszka Klimek-Kopyra
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Reference Strain ◽  
Root Nodules ◽  
Bacterial Species ◽  
Soil Science ◽  
Associated Bacteria ◽  
Plant Cultivation ◽  
Yeast Extract Mannitol ◽  
Diverse Community

SummaryThe bacterial nodulation (nod) genes are essential in the formation process of root nodules. This study was aimed to verify the occurrence of nodule-associated bacteria in two pea varieties (“Tarchalska” and “Klif ”) inoculated withRhizobiuminoculants – Nitragine™ and a noncommercial one produced by the Polish Institute of Soil Science and Plant Cultivation (IUNG). The number of colonies isolated on yeast extract mannitol (YEM) agar from the nodules of “Klif ” inoculated with IUNG inoculants was significantly higher than the number of colonies isolated from other variants. Species identification was based on sequencing of 16S rDNA, which revealed that despite careful sterilization of nodules, sequences of other bacterial species were detected. Among them, one sequence belonged toRhizobium leguminosarum(isolated from IUNG inoculant). To assess the presence of nodulation-capableRhizobium, amplification of thenodCgene was performed, which revealed that of 29 samples, 19 were positive. The remaining isolates, including reference strain and bacteria isolated from Nitragine™, lacked this gene. The results show that pea nodules harbor a very diverse community of bacteria. The lack ofnodCgene in some strains isolated from plants inoculated with Nitragine™ and with IUNG inoculant proves that even ifR. leguminosarumare abundant, they may not be efficient in nodulation.

scholarly journals SHAMOV Vladimir Nikolaevich (1882-1962). To the 135th of the birthday

2017 ◽  
Vol 10 (2) ◽  
pp. 176
Author(s):  
Alexander Alekseevich Andreev ◽  
Anton Petrovich Ostroushko
Keyword(s):  
Small Intestine ◽  
Blood Transfusion ◽  
Medical Service ◽  
Medical Institute ◽  
Medical Council ◽  
Military Medical Academy ◽  
Medical Academy ◽  
The Military ◽  
First Time ◽  
Lenin Prize

Shamov, Vladimir Nikolaevich (1882-1962) – an outstanding Soviet surgeon, neurosurgeon, transfuziolog, academician of the USSR (1945), honored scientist of the RSFSR and the Ukrainian SSR, General-Lieutenant of medical service, laureate of the Lenin prize (1962); awarded the order of Lenin (twice), red banner (twice), red banner, red Star and medals of the USSR. Born may 22, 1882 in Menzelinsk, Ufa governorate (now Tatarstan). In 1908 he graduated from the Military medical Academy. In 1911 he defended his doctoral thesis on the topic: "the importance of physical methods for surgery of malignant tumors". From 1914 to 1923 V. N. Shamov – senior assistant in the Department of Fedorov. In 1919 he received isohemagglutinins serum for the determination of blood groups and for the first time the country produced a blood transfusion given group membership. In 1923, V. N. Shamov was elected as head of the Department of surgery of the Kharkov medical Institute and the surgical clinic of the Ukrainian Institute of experimental medicine. In 1926, he reported he developed a method of complete isolation from neural connections of the small intestine, derived under the skin, and transferring it to the blood supply of the subcutaneous vessels. In 1928, V. N. Shamov proposed and successfully conducted the transfusion of cadaveric blood. In 1930, he organized the second in the USSR and in the world Institute of blood transfusion and emergency surgery, and became its Director. In 1935 he was awarded the title of honored Worker of science. In the years 1939-1958 V. N. Shamov headed the Department of hospital surgery of the Military medical Academy, he was the scientific Director of the Leningrad Institute of blood transfusion (1939-1941). During world war II – General-Lieutenant of medical service, Deputy chief surgeon of the red Army, in 1945 – the chief surgeon of the Supreme command of the far Eastern front. In October 1945, he was elected a full member of the USSR AMS. Since 1947 – was also the Director of the Leningrad research neurosurgical Institute them. A. L. Polenov, surgeon-in-chief of the RSFSR. Since 1958 Professor-consultant of the Military medical Academy. In 1962, V. N. Shamov became a laureate of the Lenin prize for development and introduction in practice of the method of preparation and use fibrinoliticescoy blood. N. Shamov for the first time in the country performed periarterial sympathectomy and surgery choroidal plexuses of the ventricles of the brain; developed method pregrading plasty of the esophagus isolated loop of the small intestine, raised the question of limitation contraindications for surgical interventions in the elderly. He was one of the first applied with the purpose of anesthesia, controlled hypotension and hypothermia anesthesia gas nitrous oxide, etc.; successfully completed one-step pankreatoduodenektomiyu in pancreatic cancer; described the clinical picture of tumors of cortex and medulla of the adrenal glands. V.N. Shamov was a member of the Board of the all-Union society of surgeons and the International Association of surgeons, Chairman of the Surgical society. N.I.Pirogov, the Chairman of the organizing Bureau of the 24th all-Union Congress of surgeons, member of the scientific medical Council of Ministry of health of the USSR. More than 20 of his students became heads of departments of medical Universities. V. N. Shamov awarded the order of Lenin twice red banner (twice); the red banner of Labour, red Star, medals of the USSR. Died V.N. Shamov in Leningrad on 30 March 1962. In memory of academician V. N. The Shamov in St. Petersburg on the building of the Military medical Academy and Neurosurgical Institute. Professor A. L. Polenov installed a memorial plaque, a bust of Lieutenant General of medical service V. N. Shamova installed in the courtyard of the St. Petersburg blood transfusion center, one of the streets of the city of Menzelinsk were named after academician V. N. Shamova.

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