scholarly journals CLINICAL SIGNIFICANCE OF STREPTOCOCCUS MEMBERS IN DEVELOPING PERIODONTITIS

2021 ◽  
Author(s):  
I. V. Bazhutova ◽  
D. D. Ismatullin ◽  
A. V. Lyamin ◽  
D. A. Trunin ◽  
A. V. Zhestkov ◽  
...  
Keyword(s):  
Proteolytic Enzymes ◽  
Infectious Agent ◽  
Complex Problem ◽  
Protective Role ◽  
Pathological Process ◽  
Research Literature ◽  
Protective Function ◽  
Actinomyces Naeslundii ◽  
Fibrinogen Binding ◽  
Wide Range

Bacteria of the genus Streptococcus are one of the most numerous and diverse representatives in the normal biocenosis of human organs and systems particularly being abundant as obligatory inhabitants of the oral cavity. All streptococci are divided into six groups: S.mitis, S.anginosus, S.salivarius, S.mutans, S.bovis and S.pyogenic groups, among which their certain number may potentially participate in the infectious process of developing periodontitis. Owing to the presence of a wide range of adhesion, invasion and colonization factors, they are capable of performing a protective function such as, e.g., colonization resistance, but they may also cause formation of a pathological process in the tooth tissues and dento-facial system. The most prominent adhesion factors are antigens I / II (Ag I / II), fibronectin, collagen, laminin, fibrinogen binding proteins, serine-rich glycoproteins, pili, protein M, proteases, C5a peptidases, and the presence of a tooth capsule. Among the complex of proteolytic enzymes, it is important to note that streptococci contain enzymes hyaluronidase and lyase, which cleave the β1,4 bond between N-acetylglucosamine and d-glucuronic acid as the components of hyaluronic acid being a part of the connective tissues. The members of the S.anginosus group are able to release chondroitin sulfatase, which destroys chondroitin sulfates as specific components in cartilage, ligaments and other connective tissue structures. The enzymes noted contribute to a deeper spread of microorganisms in host tissues. Pathological processes associated with the development of periodontitis comprise a complex problem, wherein several important elements take part, including an infectious agent, a macroorganismal response in the form of nonspecific and adaptive immunity, as well as involvement of anti-inflammatory components. A great body of studies in research literature are dedicated to describe to participation of the members within the "red, orange and green" complexes as the principal components in developing periodontitis. Whereas the "yellow" and the "purple" complex plays a more protective role by acting as antagonists while interacting with periodontopathogens, but it should not be ruled out a potential participation for some representatives, particularly S. intermedius, S. gordonii, Actinomyces odontolyticus, Actinomyces naeslundii in developing periodontal disease. Altogether, it poses a problem, which may be solved solely based on a multidisciplinary approach by inviting not only dentists and bacteriologists but also researchers of other specialties. Here we review the studies found in international and national data bases such as Scopus, Web of Science, Springer, RINC.

Abstract 137: Deficiency of Senescence Marker Protein 30 Exacerbates Doxorubicin-Induced Cardiac Dysfunction in Mice

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Makiko Miyata ◽  
Satoshi Suzuki ◽  
Tomofumi Misaka ◽  
Shu-ichi Saitoh ◽  
Yasuchika Takeishi
Keyword(s):  
Cardiac Dysfunction ◽  
Protective Role ◽  
Left Ventricular ◽  
Left Ventricular Ejection ◽  
Marker Protein ◽  
Morphological Examination ◽  
Protective Function ◽  
Ventricular Ejection Fraction ◽  
Wide Range ◽  
Oxidative Effects

Background: Senescence marker protein 30 (SMP30) was originally identified as an aging marker protein in the rat liver. The expression of SMP30 decreases with aging androgen-independently. Doxorubicin (DOX) has been used as a potent anticancer agent, but serious cardiotoxicity precludes its use in a wide range of patients. SMP30 may have anti-oxidative and anti-apoptosis functions in several organs, but functional role of SMP30 has not been rigorously examined in the heart. We hypothesized that SMP30 has cardio-protective function by anti-aging and anti-oxidant effects on DOX-induced cardiac dysfunction. Method and Results: Male SMP30 knockout (SMP30KO) and age-matched littermate male wild-type (WT) mice at 12-14 weeks of age were given intraperitoneal injections of DOX (20 mg/kg) or saline. Seven days after DOX injection, echocardiography revealed that left ventricular ejection fraction in DOX-treated SMP30KO mice was more severely reduced than in DOX-treated WT mice (40.9 ± 3.1% vs. 46.9 ± 4.9%, P<0.01). Morphological examination of myocardial sections showed fibrotic change in DOX-treated SMP30KO mice significantly increased compared to DOX-treated WT mice (3.2 ± 0.5% vs. 1.3 ± 0.2%, P<0.01). Generation of reactive oxygen species assessed by dihydroethidium staining was greater in DOX-treated SMP30KO mice than DOX-treated WT mice (166.9 ± 8.6% vs. 131.6 ± 5.8%, P<0.01). Moreover, apoptotic signaling pathways such as caspase-3 activity (1.8 ± 0.1% vs. 1.1 ± 0.2%, P<0.01), bax/bcl-2 ratio (2.4 ± 0.3% vs.1.6 ± 0.2%, P<0.05) and phosphorylation activity of c-Jun N-terminal kinase (1.6 ± 0.3 vs. 1.0 ± 0.1, P<0.05) were significantly elevated in the SMP30KO mice compared with WT mice after DOX injection. The numbers of TUNEL-positive nuclei in the myocardium were higher in DOX-treated SMPKO mice than in DOX-treated WT mice (0.15 ± 0.02% vs. 0.08 ± 0.01%, P<0.01). Conclusions: The results of this study demonstrated that DOX-induced cardiotoxicity is aggravated in SMP30KO mice by exacerbating of superoxide generation, leading to enhanced apoptosis of cardiomyocytes. SMP30 has a cardio-protective role by anti-apoptotic and anti-oxidative effects in DOX-induced cardiotoxicity.

scholarly journals Individual Expression of Hepatitis A Virus 3C Protease Induces Ferroptosis in Human Cells In Vitro

2021 ◽  
Vol 22 (15) ◽  
pp. 7906
Author(s):  
Alexey A. Komissarov ◽  
Maria A. Karaseva ◽  
Marina P. Roschina ◽  
Andrey V. Shubin ◽  
Nataliya A. Lunina ◽  
...  
Keyword(s):  
Cell Death ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Proteolytic Enzymes ◽  
Morphological Changes ◽  
Human Cells ◽  
Mitochondrial Potential ◽  
3C Protease ◽  
Wide Range

Regulated cell death (RCD) is a fundamental process common to nearly all living beings and essential for the development and tissue homeostasis in animals and humans. A wide range of molecules can induce RCD, including a number of viral proteolytic enzymes. To date, numerous data indicate that picornaviral 3C proteases can induce RCD. In most reported cases, these proteases induce classical caspase-dependent apoptosis. In contrast, the human hepatitis A virus 3C protease (3Cpro) has recently been shown to cause caspase-independent cell death accompanied by previously undescribed features. Here, we expressed 3Cpro in HEK293, HeLa, and A549 human cell lines to characterize 3Cpro-induced cell death morphologically and biochemically using flow cytometry and fluorescence microscopy. We found that dead cells demonstrated necrosis-like morphological changes including permeabilization of the plasma membrane, loss of mitochondrial potential, as well as mitochondria and nuclei swelling. Additionally, we showed that 3Cpro-induced cell death was efficiently blocked by ferroptosis inhibitors and was accompanied by intense lipid peroxidation. Taken together, these results indicate that 3Cpro induces ferroptosis upon its individual expression in human cells. This is the first demonstration that a proteolytic enzyme can induce ferroptosis, the recently discovered and actively studied type of RCD.

scholarly journals Albizia chinensis bark extract ameliorates the genotoxic effect of cyclophosphamide

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Marian Nabil ◽  
Entesar E. Hassan ◽  
Neven S. Ghaly ◽  
Fawzia A. Aly ◽  
Farouk R. Melek ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Inhibitory Effect ◽  
Protective Role ◽  
Bark Extract ◽  
Repeated Treatment ◽  
Mouse Bone Marrow ◽  
Sperm Abnormalities ◽  
Wide Range ◽  
Different Doses

Abstract Background The genus Albizia (Leguminoseae) is used in folk medicine for the treatment of a wide range of ailments. Recently, saponins from plant origin have attracted much attention. Saponins are recorded to have a broad range of biological and pharmacological activities. This study was performed to evaluate the protective role of Albizia chinensis bark methanolic extract (MEAC) against the genotoxicity induced by cyclophosphamide (CP) using different mutagenic parameters. Results The results showed that MEAC induced an inhibitory effect against chromosomal aberrations of CP in mouse bone marrow and spermatocytes. Such effect was found to be significant (p < 0.01) with a dose of 100 mg/kg treated once for 24 h and also after repeated treatment at a dose of 25 mg/kg for 7 days. In sperm abnormalities, the protective effect of Albizia extract showed a dose-related relationship. Different doses of MEAC (25, 50, and 100 mg/kg) significantly (p < 0.01) ameliorated sperm abnormalities induced by CP dose-dependently. The percentage of sperm abnormalities was decreased to 5.14 ± 0.72 in the group of animals treated with CP plus MEAC (100 mg/kg) indicating an inhibitory effect of about 50%. Conclusion MEAC at the doses examined was non-genotoxic compared to control (negative) and exhibited a protective role against CP genotoxicity.

STUDYING OF ACTIVITY OF INHIBITORS OF PROTEOLYTIC ENZYMES IN PRODUCTS OF PROCESSING OF GRAIN HARICOT (PHASEOLUS VULGARIS)

2018 ◽  
Author(s):  
Н.Т. ШАМКОВА ◽  
А.М. АБДУЛХАМИД
Keyword(s):  
Phaseolus Vulgaris ◽  
Liquid Medium ◽  
Proteolytic Enzymes ◽  
Trypsin Inhibitors ◽  
Milk Whey ◽  
Fine Grinding ◽  
Bean Flour ◽  
Wide Range ◽  
Mashed Potatoes

Определено содержание ингибиторов протеолитических ферментов в фасолевой муке, в пюре из зерновой фасоли, сваренной в воде, и в пюре из зерновой фасоли, сваренной после замачивания в воде в молочной сыворотке. Обосновано использование молочной сыворотки в качестве жидкой среды для варки зерновой фасоли после замачивания. Разработана технология полуфабриката в виде фасолевого пюре, предусматривающая замачивание фасоли в воде, варку в молочной сыворотке, грубое измельчение доведенной до готовности фасоли, последующее тонкое измельчение и охлаждение. Установлено, что в фасолевом пюре активность ингибиторов трипсина значительно ниже, чем в муке из фасоли, что делает пюре более предпочтительным полуфабрикатом для производства широкого ассортимента кулинарной продукции. The content of inhibitors of proteolytic enzymes in bean flour, in puree from beans harvested in water and in puree from cereal beans welded in milk whey after soaking in water is determined. The use of whey as a liquid medium for cooking grain beans after soaking is substantiated. The technology of semi-finished product in the form of bean puree, providing for soaking beans in water, cooking in milk whey, coarse grinding of the bean brought to the ready, subsequent fine grinding and cooling is developed. It has been found that the activity of trypsin inhibitors in bean puree is much lower than in bean flour, which makes mashed potatoes a more preferred semi-finished product for the production of a wide range of culinary products.

scholarly journals AUTOPSY FEATURES AT THE ACUTE DESTRUCTIVE PANCREATITIS

2020 ◽  
Vol 28 (2) ◽  
pp. 48-54
Author(s):  
Al'fiya K. Imayeva ◽  
Tagir I. Mustafin
Keyword(s):  
Acute Pancreatitis ◽  
Transverse Colon ◽  
Infectious Agent ◽  
Pathological Process ◽  
Postmortem Examination ◽  
Retroperitoneal Space ◽  
Contrast Study ◽  
Type I ◽  
Type Iii ◽  
The Right

In all variants of the destructive form of pancreatitis, the involvement of adipose tissue of both para-pancreatic and distant localization was noted. In this case, it is necessary to improve the pathological diagnosis of acute destructive pancreatitis. The aim of the study was to develop new techniques for postmortem examination of those who died from complications of acute pancreatitis. Pathological studies were supplemented by the methods proposed by the authors, which made it possible to clarify the spread of the infectious agent through the retroperitoneal tissue. The scheme proposed by the authors for recording changes in the tissue of the pancreas and surrounding tissues after a contrast study made it possible to clarify the main ways of propagation of the purulent-necrotic process along the retroperitoneal tissue. The results of postmortem examination of 67 deaths from complications of acute pancreatitis are presented. Based on the study, 5 types of acute destructive pancreatitis were identified. In type I, the pathological process involves the right half of the retroperitoneal tissue, including the mesentery root of the small intestine, the para-pancreatic region, the right para-kidney and sub-hepatic space. The second type is characterized by the presence of inflammatory and destructive changes in the fiber of the right lateral canal, the actual retroperitoneal space on the right and the mesentery root of the transverse colon. Purulent-necrotic changes in para-pancreatic, left perineal tissue, mesentery of the transverse colon, also in peritoneum were typical for type III acute destructive pancreatitis. For type IV disease, in addition to areas of involvement as in type III, involvement of the pelvic tissue and the left sections of the retroperitoneal space itself were noted. The spread of the infectious agent to both the right and left parts of the retroperitoneal tissue was defined as type V acute destructive pancreatitis.

scholarly journals Analysis of the Powertrain Component Size of Electrified Vehicles Commercially Available on the Market

2022 ◽  
Vol 24 (1) ◽  
pp. B74-B86
Author(s):  
Ruzimov Sanjarbek ◽  
Jamshid Mavlonov ◽  
Akmal Mukhitdinov
Keyword(s):  
Mass Production ◽  
Combustion Engine ◽  
Research Literature ◽  
Component Size ◽  
Performance Requirements ◽  
Vehicle Data ◽  
Wide Range ◽  
Insight Into ◽  
Real Vehicle ◽  
Electrified Vehicles

The paper aims to present an analysis of the component sizes of commercially available vehicles with electrified powertrains. The paper provides insight into how the powertrain components (an internal combustion engine, an electric motor and a battery) of mass production electrified vehicles are sized. The data of wide range of mass production electrified vehicles are collected and analyzed. Firstly, the main requirements to performance of a vehicle are described. The power values to meet the main performance requirements are calculated and compared to the real vehicle data. Based on the calculated values of the power requirements the minimum sizes of the powertrain components are derived. The paper highlights how the sizing methodologies, described in the research literature, are implemented in sizing the powertrain of the commercially available electrified vehicles.

scholarly journals Hepatic involvement in sarcoidosis

2019 ◽  
Vol 91 (4) ◽  
pp. 4-12
Author(s):  
V V Fomin ◽  
M Yu Brovko ◽  
M V Kalashnikov ◽  
V I Sholomova ◽  
T P Rozina ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
Normal Liver ◽  
Liver Function Tests ◽  
Pathological Process ◽  
Unknown Etiology ◽  
Primary Biliary Cholangitis ◽  
Treatment Protocols ◽  
Hepatic Involvement ◽  
Wide Range ◽  
Hepatic Sarcoidosis

Sarcoidosis is a multisystem granulomatous disease of unknown etiology. Liver is frequently involved in the pathological process. Wide range of clinical manifestations can be seen: from asymptomatic lesion with normal liver function tests to cirrhosis with portal hypertension. Biopsy plays the key role in diagnosis of the hepatic sarcoidosis. It is essential for morphological diagnosis to exclude other causes of granulomatous liver disease, most often - primary biliary cholangitis. Nowadays there are no standard treatment protocols for patients with hepatic sarcoidosis.

scholarly journals Smart EcoCUVA – Tools for Food Marketing Strategy

2006 ◽  
pp. 1-8
Author(s):  
Maarit Pallari
Keyword(s):  
Decision Making ◽  
Action Research ◽  
Well Being ◽  
Complex Problem ◽  
Utility Value ◽  
Value Analysis ◽  
Trade Offs ◽  
Wide Range ◽  
The Usa ◽  
Application Fields

The implementation of the green productivity and marketing concept in agribusiness is a must in the future.Food production is influenced by the environment and society, and vice versa. Today a growing number ofconsumers are aware of the link between environmental and social well-being and fresh, pure, healthy, tastyand safe foodstuffs. Enterprises will have to consider three important aspects of value when doing business:economic, social and environmental value. The foundation of the quality research this study is concernedwith is action research. Action research is a way to analyze sustainable development, the aptness ofagriculture and the marketing opportunities these offer for developing ecoproducts in the SMEs.The study seeks to answer the following questions:- What kind of Classical Utility Value Analyses could be tool of the eco-product?- To what extent can a customer/interest group affect the development and decision-making of ecoproducts?- Is the method a suitable tool for analyzing ecological criterions in the marketing model?Classical Utility Value Analysis is a formal, analytic approach for evaluating and comparing differentalternatives. It is one decision making method of multi-criteria analysis. The roots of utility value analysis,which is one of the mathematical models for analytical decision making, are in the USA and Germany. Themethod is almost 40 years old, of the same age as the manuscript of the values tradeoff. The same historicallanguage is being used to build up new tools, principles and theory. The so-called Smart EcoCUVA hasused both methodologies (Utility value analysis and decision making analysis) when setting the goals andmathematical steps. Research results always give the best available alternative.Making rational decisions for any complex problem requires various analyses of trade-offs (compromises)between conflicting goals (objectives, outcomes) that are used for measuring the results of applying variousdecisions in a wide range of application fields. A typical decision problem has an infinite number ofsolutions, and decision makers are interested in analyzing trade-offs between those that correspond to theirpreferences, which is often called a preferential structure of the decision-maker.Smart EcoCuva analysis helps to assess different alternatives according to a variety of environmentalcriteria associated with enterprises and their products. The analysis methods take account of the monetaryand non-monetary aspects when determining the selection of the best alternative. Smart EcoCuva is themethodological cornerstone for the creation of an innovative concept that will contribute to encouraging theefficient use of natural resources and thereby enhancing sustainability.The Smart EcoCuva tools to be developed aim to be environmentally sound, economically viable, sociallyjust and culturally appropriate. They are a new, science-based reaction of sustainable agriculture to globalatmosphere, as well as constitute an appropriate link between people and nature. New environmentallyfriendly food combines healthy food with people’s lifestyles.

scholarly journals Digestive Alkaline Proteases from Zosterisessor ophiocephalus, Raja clavata, and Scorpaena scrofa: Characteristics and Application in Chitin Extraction

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Rim Nasri ◽  
Islem Younes ◽  
Imen Lassoued ◽  
Sofiane Ghorbel ◽  
Olfa Ghorbel-Bellaaj ◽  
...  
Keyword(s):  
Proteolytic Enzymes ◽  
Tween 20 ◽  
Sodium Perborate ◽  
Alkaline Proteases ◽  
Shrimp Waste ◽  
Wide Range ◽  
Raja Clavata ◽  
Shrimp Wastes ◽  
Scorpaena Scrofa ◽  
Zosterisessor Ophiocephalus

The aim of this work was to study some biochemical characteristics of crude alkaline protease extracts from the viscera of goby (Zosterisessor ophiocephalus), thornback ray (Raja clavata), and scorpionfish (Scorpaena scrofa), and to investigate their applications in the deproteinization of shrimp wastes. At least four caseinolytic proteases bands were observed in zymogram of each enzyme preparation. The optimum pH for enzymatic extracts activities of Z. ophiocephalus, R. clavata, and S. scrofa were 8.0-9.0, 8.0, and 10.0, respectively. Interestingly, all the enzyme preparations were highly stable over a wide range of pH from 6.0 to 11.0. The optimum temperatures for enzyme activity were 50∘C for Z. ophiocephalus and R. clavata and 55∘C for S. scrofa crude alkaline proteases. Proteolytic enzymes showed high stability towards non-ionic surfactants (5% Tween 20, Tween 80, and Triton X-100). In addition, crude proteases of S. scrofa, R. clavata, and Z. ophiocephalus were found to be highly stable towards oxidizing agents, retaining 100%, 70%, and 66%, respectively, of their initial activity after incubation for 1 h in the presence of 1% sodium perborate. They were, however, highly affected by the anionic surfactant SDS. The crude alkaline proteases were tested for the deproteinization of shrimp waste in the preparation of chitin. All proteases were found to be effective in the deproteinization of shrimp waste. The protein removals after 3 h of hydrolysis at 45∘C with an enzyme/substrate ratio (E/S) of 10 were about 76%, 76%, and 80%, for Z. ophiocephalus, R. clavata, and S. scrofa crude proteases, respectively. These results suggest that enzymatic deproteinization of shrimp wastes by fish endogenous alkaline proteases could be applicable to the chitin production process.

scholarly journals Release of endogenous chondroitin sulfate and heparin as consequence of dysregulated proteolysis in COVID-19

2021 ◽  
Author(s):  
Marco Ruggiero
Keyword(s):  
Adverse Effect ◽  
Platelet Aggregation ◽  
Chondroitin Sulfate ◽  
Plasma Proteins ◽  
Adverse Reactions ◽  
Proteolytic Enzymes ◽  
Protective Role ◽  
Platelet Factor ◽  
Sequential Release ◽  
Immune Mediated

Abstract Infection by severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), the pathogen responsible for COVID-19, is associated with immune-mediated responses that lead to dysregulated activation of proteolytic enzymes; these contribute to damage to the endothelium, thrombosis, hypercoagulability, and other hematologic complications that include thrombotic thrombocytopenia, a complication of severe COVID-19 as well as a potentially fatal adverse effect of COVID-19 vaccination. Here, it is demonstrated that proteolysis of plasma proteins leads to sequential release of endogenous glycosaminoglycans (GAGs), first chondroitin sulfate (CS), followed by heparin (HP). The extension and degree of what is called "proteolytic storm" determines whether only one endogenous type of GAGs (CS), or both (CS and HP), are released. Sulfated GAGs such as CS and HP exert a protective role against SARS-CoV-2 infection. However, sustained and excessive release of endogenous HP may be responsible for thrombotic thrombocytopenia just as it happens in HP-induced thrombocytopenia (HIT) a well-known side effect of HP administration that results in thromboembolisms in atypical sites, thrombocytopenia, and synthesis of autoantibodies directed against platelet factor 4 (PF4) that contribute to platelet aggregation. It is concluded that release of endogenous HP as consequence of dysregulated proteolysis occurring during COVID-19 or COVID-19 vaccination may play a fundamental role in the pathophysiology of the disease as well as in adverse reactions to vaccination.

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