scholarly journals Evaluation of the Prevalence of Subclinical Mastitis in Dairy Cattle in the Soummam Valley (Bejaia, Algeria)

2019 ◽  
Vol 76 (2) ◽  
pp. 143
Author(s):  
Asma Ait KAKI ◽  
Salem DJEBALA ◽  
Muhammad Bilal LATIF ◽  
Nassim MOULA
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Dairy Cattle ◽  
Dairy Cows ◽  
Farming System ◽  
Subclinical Mastitis ◽  
Bacterial Strains ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Bacterium Species

The present study was carried out to investigate the prevalence of subclinical mastitis (SCM) in milking cows and the effect of farming system on the prevalence of SCM”, as well as the identification of bacterial strains causing the mammary gland infection. A total of 100 dairy cows were randomly selected from 32 farms of Bejaia district (Algeria), precisely the Soummam region: from Tazmalt to El Kseur. The SCM was screened by California Mastitis Test (CMT). Milk samples were collected aseptically from (CMT) positive cows and dispatched to laboratory for further microbiological tests. Results showed that the prevalence of SCM was 26%. Moreover, no significant differences in SCM prevalence were observed between intensive and semi intensive systems (P>0.05). The Staphylococcus aureus was found to be the most frequent bacterium species associated with SCM in the cows studied.

Prevalence of Subclinical Mastitis in some Dairy Cattle Farms in Kuwait

2019 ◽  
Vol 20 (2) ◽  
pp. 79-85
Author(s):  
A AbdelKhalek ◽  
M El-sherbini ◽  
S Alanzi
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Single Infection ◽  
Bacterial Strains ◽  
E Coli ◽  
Milk Samples ◽  
Staphyloccocus Aureus ◽  
Cattle Farms ◽  
Apparently Healthy

A survey to prevalence of subclincal mastitis (SCM) in some dairy cattle farms in state of Kuwait was carried out. 200 quarter milk samples from apparently healthy dairy cows. 200 quarter milk samples from apparently healthy dairy cows at Kuwait were investigated for subclinical mastitis using California mastitis test (CMT), Somatic cell count (SCC) and bacteriological isolation. The results revealed that the prevalence of subclinical mastitis was 45 % and bacterial culture was positive in 37.5%. SCC was assessed in 200 milk samples, the minimum and maximum SCC was 0.12 x 105 cell/ ml and 4.75 x 105 cell/ ml with the mean of 1.7±18.11 x 105 cell/ ml. The cultural examinations to determine subclinical mastitis causative agents revealed that the isolated bacterial strains in examined milk samples of cows were Staphyloccocus aureus, Streptococcus agalactia, E. coli and Pseudomanas aeruginosa with distribution of 37.6%, 20.5%, 19.4% and 3.2%, respectively as single infection from total 93 isolated bacterial strains. Meantime, there were mixed infection of (Staphyloccocus aureus + E. coli); (Streptococcus agalactia + E. coli) and (Staphyloccocus aureus + Pseudomanas aeruginosa) with the recovery rates of distribution of 8.6%, 9.7%, and 1%, respectively. Antibiogram profile of some representative field strains of Staphyloccocus aureus, Streptococcus agalactia, E. col and Pseudomanas aeruginosa isolated from subclinical mastitis cases to 11 different antibiotics. It revealed that all Staphyloccocus aureus, Streptococcus agalactia E. coli and Pseudomanas aeruginosa isolates were highly sensitive to enrofloxacillin, and Cloxacillin. However, it showed variable sensitivity degrees to other tested antibiotics. It could be concluded that subclinical mastitis constitutes a major economic and healthy problem for dairy herd in Kuwait.

scholarly journals Molecular characterization of virulence factors in Staphylococcus aureus isolated from bovine subclinical mastitis in central Ethiopia

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Desiye Tesfaye Tegegne ◽  
Gezahegne Mamo ◽  
Hika Waktole ◽  
Yohannes Equar Messele
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Animal Health ◽  
Subclinical Mastitis ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Lactating Dairy Cows ◽  
Genomic Study

Abstract Purpose Staphylococcus aureus (S. aureus) is the most important pathogen involved in bovine mastitis in dairy production. S. aureus produces a spectrum of extracellular protein toxins and virulence factors which are thought to contribute to the pathogenicity of the organism. The aim of this work was to isolate and molecular characterize S. aureus associated with bovine subclinical mastitis in the central part of Ethiopia. Methods A total of 265 lactating dairy cows from various dairy farms in four different geographical locations were screened by the California mastitis test (CMT) for bovine subclinical mastitis. One hundred thirty CMT-positive milk samples were collected and transported to the laboratory. Different biochemical tests and polymerase chain reaction (PCR) were used for the identification of S. aureus isolates. Finally, PCR was performed for molecular detection of virulence genes. Results From a total of 265 lactating dairy cows screened, 49% (n = 130) were positive for bovine subclinical mastitis. One hundred thirty mastitic milk samples were subjected to bacterial culturing, and one hundred (76%) S. aureus isolates were identified based on phenotypic characters. Sixty-eight confirmed S. aureus isolates were obtained using PCR. The confirmed S. aureus isolates were tested for six virulence genes (tsst-1, hlb, eta, sea, clfA, and icaD) using PCR. Of the six virulence genes screened from all the isolates, only two (clfA and eta) were detected in the isolates. Out of 68 isolates, 25% and 22% were possessed the eta and clfA genes, respectively. Conclusion The presence of Staphylococcus aureus having virulence genes (eta and clfA) revealed that mastitis is a major concern nowadays affecting animal health, milk quality, and yield. Further genomic study of these isolates will provide broad new insights on virulence.

scholarly journals THE OCCURRENCE OF BACTERIAL AGENTS CAUSING MASTITIS IN DAIRY SHEEP AND THEIR RESISTANCE TO ANTIBIOTICS

2020 ◽  
Vol 3 (7) ◽  
Author(s):  
František Zigo ◽  
Katarína Veszelits Lakticová ◽  
Mária Vargová M
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
High Resistance ◽  
Subclinical Mastitis ◽  
Causative Organism ◽  
Coagulase Negative Staphylococci ◽  
Isolation And Identification ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Acute Form

Introduction: The aim of this study was to monitor the occurrence of bacterial agents causing mastitis, forms of mastitis and antibiotic resistance in 300 ewes on a farm in Eastern Slovakia. Material and methods: During the milking season, were performed three complex investigations including clinical examination, California Mastitis Test and laboratory analysis of milk samples. The investigations and milk samples were taken in three phases; the beginning (April), the middle (June) and the end (September) of the milking season. Results: Of all the samples (806), 225 (28.0%) were positive for bacterial pathogens. The highest incidence of mastitis (33.3%) was recorded in September, while April (23.8%) and June (25.3%) had lower incidence. The samples from September showed the highest incidence of subclinical mastitis (20.1%), with 13.2% being an acute form of mastitis. Coagulase-negative staphylococci (CNS) were identified in 61.7% of the positive samples. Especially, S. chromogenes, S. epidermidis and S. schleiferi were most frequently isolated.  Staphylococcus aureus was the causative agent in 20.0% of the positive samples and caused acute or subclinical mastitis in the affected ewes. The tested bacteria showed very high resistance to novobiocin (59.5%) and penicillin (51.4%) and high resistance to amoxycillin (35.1%). We found that 80% of Staphylococcus aureus bacteria tested for antibiotic resistance were resistant to novobiocin and 70% were resistant to penicillin. Of all tested CNS, 56.5% were resistant to novobiocin, 39.1% to penicillin, and 34.7% to amoxicillin. Conclusion: Proper isolation and identification of the causative organism play a significant role in the prevention and control of the intramammary infection. In our study, a combinations of Streptomycin, Ciprofloxacin and Tetracycline were the most effective antibiotics for the control of mastitis.

A COMPARISON OF MASTITIS DETECTION METHODS IN DAIRY CATTLE

1984 ◽  
Vol 64 (2) ◽  
pp. 305-312 ◽  
Author(s):  
T. R. BATRA ◽  
A. J. McALLISTER
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Good Accuracy ◽  
Subclinical Mastitis ◽  
Detection Methods ◽  
Considerable Proportion ◽  
Milk Samples ◽  
California Mastitis Test

The potential value of electrical conductivity, somatic cell count, and California Mastitis Test (CMT) Score in composite milk samples as a screening test for subclinical mastitis was evaluated. The effect of genetic group was significant (P < 0.05) for conductivity and somatic cell count while the effects of parity and month of lactation were significant (P < 0.01) for all the three traits studied. Somatic cell count and CMT score increased from first to fourth parity while there was general trend for increasing conductivity from second to fourth parity. All the three methods identified healthy cows with a good accuracy but failed to recognize a considerable proportion of infected cows. The error rate for misclassification was lowest for CMT score (9.1%), followed by somatic cell count (13.9%) and conductivity (29.4%). Composite strict foremilk samples from cows infected with primary pathogens had significantly (P < 0.01) higher conductivity than those from uninfected cows. Staphylococcus aureus were isolated in 60% of the milk samples and appeared to be in the major organism causing mastitis in this herd. Key words: Dairy cattle, mastitis detection methods

scholarly journals The Impact of Mastitis on Reproductive Parameters of Dairy Cows

2019 ◽  
Vol 63 (3) ◽  
pp. 72-78
Author(s):  
F. Zigo ◽  
A. Adamová ◽  
M. Vasiľ ◽  
J. Elečko ◽  
S. Ondrašovičová ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reproductive Parameters ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Service Period ◽  
High Incidence ◽  
The Impact

Abstract The aim of this study was to evaluate the occurrence of mastitis and its impact on the reproductive parameters in a herd of 180 dairy cows. Based on the herd records 127 cows of Slovak spotted cattle and their crosses with red Holstein were selected for study between 1—2 months after calving. The examination of the health status of the mammary glands consisted of: the clinical examination of the udder, the California mastitis test (CMT) supplemented by the collection of mixed milk samples, and the laboratory examination of bacterial pathogens causing the mastitis. In addition to the mam­mary investigation, reproduction indicators such as the length of the insemination interval, the service period, the intercalving period and the insemination index were also analyzed. The results of this study indicated: a high incidence of mastitis (41.6 %), especially latent (21.2 %), subclinical (15.7 %) and clinical (4.7 %) forms were most common in the herd. The most frequently isolated bacteria from the infected milk samples were: coagulase negative staphylococci (54.1 %), S. aureus (16.9 %), Streptococcus spp. (15.0 %), A. viridans (7.5 %) and Ent. faecalis (6.4 %). According to the available literature, the optimum values of the intercalving period were 365—400 days, the insemination interval 55—80 days, the insemination index 1.2—2 and the service period 60—110 days. In comparison, our results showed increased, unsatisfactory reproductive values in the group of dairy cows with clinical mastitis. While in healthy cows as well as in groups of cows with latent and subclinical mastitis, all of the reproductive indicators were within the optimal levels.

scholarly journals Staphylococcus aureus isolates from bovine subclinical mastitis in central Ethiopia revealed the presence of antimicrobial resistance and resistant genes

2021 ◽  
Author(s):  
Desiye Tesfaye Tegegne ◽  
Gezahegne Mamo ◽  
Hika Waktole ◽  
Gebrerufael Girmay
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Resistance Genes ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Economic Losses ◽  
Central Ethiopia ◽  
Milk Samples ◽  
Lactating Dairy Cows

Abstract Background: Staphylococcus aureus is one of the predominant causative agents of mastitis disease in dairy herds. Mastitis disease has a negative impact in the economic losses in the dairy sector across the globe. The aims of this study were to determine the prevalence and detect antimicrobial resistance genes in the Staphylococcus aureus isolated from milk samples of subclinical bovine mastitis in Central Ethiopia.Methods: A total of 265 lactating dairy cows from various dairy farms in four different geographical locations were screened by California mastitis test (CMT) for bovine subclinical mastitis. One-hundred thirty CMT positive milk samples were collected and transported to laboratory. Different biochemical tests and polymerase chain reaction (PCR) were used for the identification of S. aureus isolates. Finally, phenotypic and genotypic methods were performed for detection of some antimicrobial resistance patterns and genes (mecA, ermA, ermC, and msrA), respectively. Results: From total of 265 lactating dairy cows screened, 49% (n=130) were positive for bovine subclinical mastitis. One-hundred thirty mastitic milk samples were subjected to bacterial culturing, one hundred (76%) S. aureus isolates were identified based on phenotypic characters. Sixty-eight confirmed S. aureus isolates were obtained using PCR. Of the sixty-eight isolates tested 12 samples were contained the methicillin resistance gene A (mecA). No amplification was observed for the erythromycin resistance genes (ermA, ermC, and msrA). Conclusion: The high resistance of Staphylococcus aureus to commonly used antimicrobials contribute in dairy farms may cause health problems in the community consuming raw milk purchased from these farms.

Subclinical mastitis in dairy cows in Swiss organic and conventional production systems

2006 ◽  
Vol 74 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Markus Roesch ◽  
Marcus G Doherr ◽  
Walter Schären ◽  
Melchior Schällibaum ◽  
Jürg W Blum
Keyword(s):  
Dairy Cows ◽  
Production Systems ◽  
Post Partum ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Cell Counts ◽  
Conventional Production

The objective was to compare the prevalence of subclinical mastitis (SM) and of udder pathogens in 60 Swiss organic (OP) and 60 conventional production systems (CP). Cows (n=970) were studied for SM prevalence and udder pathogens at median 31 d and 102 d post partum. Cows showing a [ges ]1+ positive California Mastitis Test (CMT) in at least one quarter were considered to have SM. Cow-level prevalences of SM for visits at 31 d and 102 d post partum (39% and 40% in OP and 34% and 35% in CP) were similar, but quarter-level prevalences of SM were higher (P<0·02) in OP than CP (15% and 18% in OP and 12% and 15% in CP). Median somatic cell counts in milk at 31 d post partum were higher (P<0·05) in OP than CP cows (43000 and 28000 cells/ml, respectively), but were similar at 102 d post partum in OP and CP cows (45000 and 38000 cells/ml, respectively). In milk samples from quarters showing a CMT reaction [ges ]2+ the prevalences of coagulase negative staphylococci were lower (P<0·05) at 102 d post partum, whereas prevalences of non-agalactiae streptococci were higher (P<0·05) in OP than in CP cows at 31 d and 102 d post partum. In conclusion, under Swiss conditions, subclinical mastitis is a greater problem in organic than in conventional production systems, but differences are not marked.

scholarly journals Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

2015 ◽  
Vol 65 (3) ◽  
pp. 358-370 ◽  
Author(s):  
Gürler Hande ◽  
Findik Arzu ◽  
Gültiken Nilgün ◽  
Ay Serhan Serhat ◽  
Çiftçi Alper ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Culture Methods ◽  
Milk Samples ◽  
California Mastitis Test ◽  
High Prevalence ◽  
Enterococcus Spp ◽  
Molecular Identification Methods ◽  
Polymerase Chain ◽  
Jersey Cows

AbstractThe aim of this study was to investigate the etiology of subclinical mastitis (SCM) in dairy Jersey cows with the use of bacteriological and molecular identification methods. In the study 121 Jersey and 78 hybrid Jersey cows with SCM were observed in the Samsun district of Turkey. A total of 411 California mastitis test (CMT) positive milk samples from these animals were examined bacteriologically. The prevalence of subclinical mastitis was 54.75% and 67.2% in Jerseys and hybrids, respectively. On bacteriological examination, a total of 92 strains were isolated from 411 milk samples. The most prevalent bacteria wereStaphylococcusspp. (69.56%). Among them 24 isolates wereStaphylococcus aureus(26.08%) the other isolates wereStreptococcus dysgalactiae(23.91%),Enterococcus spp.(3.26%) andStreptococcus agalactiae(3.26%). All strains were identified with bacteriological culture methods, as well as by Polymerase Chain Reaction (PCR). Gram-negative bacteria were not isolated. In conclusion, the etiology of SCM in full blood and hybrid Jersey dairy cows in Samsun and the prevalence of bacteria were determined. The relatively high prevalence of SCM indicates the potential need for the consideration of some factors contributing to the formation of mastitis (e.g. management) as well as bacterial agents. The present study and further studies may be useful to develop mastitis vaccines by means of providing true vaccine strain sources.

SUBCLINICAL MASTITIS IN LACTATING COWS: COMPARISON OF FOUR SCREENING TESTS AND EFFECT OF ANIMAL FACTORS ON ITS OCCURRENCE

2016 ◽  
Vol 13 (2) ◽  
pp. 41-50 ◽  
Author(s):  
M Badiuzzaman ◽  
MA Samad ◽  
SHMF Siddiki ◽  
MT Islam ◽  
S Saha
Keyword(s):  
Dairy Cows ◽  
Predictive Value ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Standard Test ◽  
Screening Tests ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Cultural Isolation

A total of 444 quarter milk samples from 111 crossbred dairy cows were subjected to California mastitis test (CMT), somatic cell count (SCC) test, white side test (WST) and surf field mastitis (SFMT) test to quantify their efficacy in detecting sub clinical mastitis in dairy cows of Bangladesh during the period from 2010 to 2011.Milk samples positive by CMT, SCC, WST and SFMT were 265,230, 225 and 222 respectively. All samples were subjected to cultural isolation (gold standard test for comparison of indirect mastitis tests). Of these, 261 samples were positive by cultural isolation. The sensitivity of the CMT, SCC, WST and SFMT were 80.08%, 86.60%, 60.54% and 57.47%; specificity 69.40%, 97.81%, 63.38% and 60.66%; percentage accuracy 75.68%,91.22%, 61.71%  and 58.78%; positive predictive value 78.87%, 98.26%, 70.22% and 67.57%; and negative predictive value 70.95%, 83.64%, 52.97% and 50%respectively. Quarter-wise sub clinical mastitis (SCM) was detected in 59.68%, 51.80%, 50.68% and 50% samples by CMT, SCC, WST and SFMT, respectively, while animal-wise SCM was recorded in 72.07%, 66.67%, 64.86% and 61.26% samples by CMT, SCC, WST and SFMT, respectively. The right hind quarters were most significantly (p<0.001) susceptible to SCM than other quarters. Cows with 3rd and 4th parity and at their early lactation stage had significantly higher (p<0.001) SCM than others. Kappa value of SCC was higher than that of CMT. CMT was concluded to be the most accurate test after cultural isolation and SCC. Unlike laboratory tests as cultural isolation and SCC that require adequate laboratory facilities and skilled personnel, CMT is a reliable diagnostic method in field conditions.

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