scholarly journals Antioxidant Activity, Polyphenols Content and Antimicrobial Activity of Several Native Pteridophytes of Romania

2012 ◽  
Vol 40 (1) ◽  
pp. 53 ◽  
Author(s):  
Liliana Cristina SOARE ◽  
Mariana FERDES ◽  
Stefan STEFANOV ◽  
Zapriana DENKOVA ◽  
Radosveta NICOLOVA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Fresh Weight ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Brevibacterium Flavum ◽  
Methanolic Extracts ◽  
Cosmetic Ingredients ◽  
Better Than

The aim of this paper was to test the antioxidant activity, polyphenols content and antimicrobial activity of crude extracts obtained from leaves of pteridophyte species commonly found in Romania. The ORAC (Oxygen Radical Absorbance Capacity) of the investigated ferns varied between 421.90 ?mol TE (Trolox equivalents/g FW (fresh weight) in Dryopteris filix-mas and 128.18 ?mol TE/g FW in D. affinis. Methanolic extracts obtained from leaves of ferns have similar antioxidant activity to that of some medicinal plants. Polyphenols content in the leaves of ferns varies between 2340 mg Gallic acid equivalents (GAE)/100 g FW in D. filix-mas and 887 mg GAE/100 g FW in D. affinis. The correlation coefficient between ORAC and the total polyphenol content was R=0.985. This correlation suggests that phenolic compounds are major contributors to the antioxidant activity. The methanolic extract obtained from ferns inhibits the growth of Gram negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa NBJMCC1390, Salmonella abony and Gram positive Staphyllococcus aureus ATCC 25093 and Enterococcus faecalis. The highest antimicrobial activity was determined for the Dryopteris extract. The antimicrobial activity of methanolic extracts obtained from leaves of D. filix-mas and D. affinis is better than the A. filix-femina in the case of Brevibacterium flavum ATCC 14067, Sarcina sp., Bacillus cereus ATCC 1390, Saccharomyces cerevisiae and Aspergillus niger. The tested ferns could be used as cosmetic ingredients, as preservatives in food or in antimicrobial therapy.

scholarly journals INHIBITORY EFFECT OF ESSENTIAL OILS FROM SOME LAURACEAE SPECIES ON THE GROWTH OF PENICILIUM COMMUNE

2019 ◽  
Vol 9 (Special issue) ◽  
pp. 385-389
Author(s):  
Denisa Foltinová ◽  
Dana Tančinová ◽  
Miroslava Císarová
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Plant Material ◽  
Methanolic Extract ◽  
Inhibitory Effect ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Allium Species ◽  
Total Polyphenol ◽  
Wild Garlic

Wild garlic (Allium ursinum L.) is a member of the Allium genus. However, wild garlic is still unexplored. To date, there are few publications that deal with this Allium species. The samples of plant material were collected from the three areas near Bratislava (Bratislava region), Krajné (Trenčin region) and Becherov (Prešov region). The aim of this study was to analyse the content of total polyphenols (TPC) and the value antioxidant activity (AOA) in selected morphological parts of wild garlic. The samples of wild garlic were homogenized and a methanolic extract was prepared. These extracts were used for analyses. Total polyphenol content in the samples ranged from 253.19 ± 5.72 mg GAE.kg-1 FW (bulbs from area Becherov) to 1069.51 ± 5.49 mg GAE.kg-1 FW (leaves from area Krajne). The values of antioxidant activity were from 2.54 ± 0.83 (bulbs from area Krajne) to 47.98 ± 0.54 % inhibition (leaves from area Bratislava).

scholarly journals Evaluation of Anti-Tyrosinase and Antioxidant Properties of Four Fern Species for Potential Cosmetic Applications

Forests ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 179 ◽  
Author(s):  
Adrià Farràs ◽  
Guillermo Cásedas ◽  
Francisco Les ◽  
Eva Terrado ◽  
Montserrat Mitjans ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Dpph Radical ◽  
Terrestrial Plants ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Methanolic Extracts ◽  
Skin Hyperpigmentation ◽  
Fern Species ◽  
Tyrosinase Enzyme

Ferns are poorly explored species from a pharmaceutical perspective compared to other terrestrial plants. In this work, the antioxidant and tyrosinase inhibitory activities of hydrophilic and lipophilic extracts, together with total polyphenol content, were evaluated in order to explore the potential cosmetic applications of four Spanish ferns collected in the Prades Mountains (Polypodium vulgare L., Asplenium adiantum-nigrum L., Asplenium trichomanes L., and Ceterach officinarum Willd). The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, oxygen radical absorbance capacity (ORAC) and xanthine/xanthine oxidase (X/XO) assays. The potential to avoid skin hyperpigmentation was tested by inhibiting the tyrosinase enzyme, as this causes melanin synthesis in the epidermis. All ferns were confirmed as antioxidant and anti-tyrosinase agents, but interestingly hydrophilic extracts (obtained with methanol) were more potent and effective compared to lipophilic extracts (obtained with hexane). Polypodium vulgare, Asplenium adiantum-nigrum, and Ceterach officinarum methanolic extracts performed the best as antioxidants. Polypodium vulgare methanolic extract also showed the highest activity as a tyrosinase inhibitor.

scholarly journals Antimicrobial Activity, Phenolic and Antioxidant Content of Extracts from Cuajilote (Parmentiera aculeata Kunth) Fruits at Different Degrees of Ripening

2021 ◽  
Vol 65 (2) ◽  
Author(s):  
Cristina Santiago Ruiz ◽  
Viviana Noemi Nuricumbo Lievano ◽  
Mariana Gabriela Chapa Barrios ◽  
Gilber Vela Gutiérrez ◽  
Arturo Velázquez
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Aqueous Ethanol ◽  
Polyphenol Content ◽  
Methanolic Extracts ◽  
Ethanolic Extracts ◽  
Ripe Stage

Abstract. Antioxidants and phenolic compounds are involved in antioxidative processes, decreasing the activity of free radicals. Cuajilote (Parmentiera aculeata) is a crop of the state of Chiapas, Mexico, mainly used as feed for cattle, unknown the compounds that the fruit may contain, but many researches indicate that this fruit has been used for therapeutic purposes. Therefore, the purpose of the present study is the evaluation of the antioxidant, antibacterial and polyphenol content of pulp and seed of cuajilote. The recollected fruit were classified by degree of ripeness (green (G), mature green ripe (MG) and ripe (R)). The samples were dry at 40 °C. Aqueous, ethanol and methanolic extracts were obtained from degree of ripeness mentioned above.  Higher antioxidant activity was obtained from ethanolic extracts of ripe pulp, greater polyphenol content from methanolic extracts from ripe pulp and antibacterial activity in ethanolic extracts from cuajilote pulp. The pulp has a higher antioxidant activity and polyphenol concentration in the ripe stage. Resumen. Los antioxidantes y compuestos fenólicos intervienen en los procesos antioxidantes, disminuyendo la actividad de los radicales libres. El Cuajilote (Parmentiera aculeata) es un cultivo del estado de Chiapas, México, utilizado principalmente como alimento para el ganado, desconociendo los compuestos que el fruto puede contener, pero muchas investigaciones indican que el fruto se ha utilizado para fines terapéuticos. Por lo tanto, el propósito del presente estudio es la evaluación del contenido antioxidante, antibacteriano y de polifenoles de la pulpa y la semilla de cuajilote. Los frutos recogidos se clasificaron por grado de maduración (verde (G), maduro-verde-maduro (MG) y maduro (R)). Las muestras se secaron a 40 °C, los extractos acuosos, etanólico y metanólico contuvieron los compuestos de interés. Se obtuvo actividad antioxidante mayor en los extractos etanólicos de pulpa madura; un mayor contenido de polifenoles a partir de extractos metanólicos de pulpa madura y elevada actividad antibacteriana en extractos etanólicos de la pulpa de cuajilote. La pulpa presenta superior actividad antioxidante y concentración de polifenoles en la etapa madura.

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 64-67
Author(s):  
Maruthi T. Ekbote ◽  
◽  
Rajashekar K. V ◽  
Shankarappa L ◽  
Bharathi D. R. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Piper Longum ◽  
Polyherbal Formulation ◽  
Methanolic Extracts ◽  
Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals Effect of Methanolic Extract of Caryota no on Antioxidant Levels in Drosophila melanogaster

2020 ◽  
pp. 27-35
Author(s):  
Chinonye A. Maduagwuna ◽  
Simeon Omale ◽  
Monday A. Etuh ◽  
Steven S. Gyang
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Methanolic Extract ◽  
Control Group ◽  
Total Protein Content ◽  
Total Thiol ◽  
Methanolic Extracts ◽  
Centre Of Excellence ◽  
Anti Oxidant ◽  
Plateau State

Aims: To investigate the anti-oxidant activity of the methanolic extracts of Caryota no seeds in Drosophila melanogaster (DM). Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019.                                                                                                                                                     Methodology: These assays were conducted by exposing 50 flies per vial to the selected concentrations (350 mg, 400 mg and 500 mg) of the extract in 5 independent replicates for seven days while control group received distilled water. The total protein content was then determined from the supernatant of the fly homogenate. The antioxidant activity and levels of GST, CAT and total thiol were then measured. The statistical difference among test groups was presumed at P < .05.                                                                                                         Results: The methanolic extract of Caryota no caused nonsignificant (P = .33) decrease in total proteins in DM below basal levels in a dose-dependent pattern.  The antioxidant activity showed nonsignificant (P = .28) lowering of the GST activity in DM below control levels. The methanolic extract of CN nonsignificantly (P > .05) increased the levels of catalase (P = .36) and total thiol levels (P = .22).                                                                                                       Conclusion: It can therefore be concluded that the methanolic extract of Caryota no contains appreciable concentrations of different types of antioxidants. This may provide perspectives for the evaluation and development of effective and safe phytomedicines created from the local biodiversity. 

scholarly journals Photoprotection and Photostability of a New Lignin-Gelatin-Baccharis antioquensis-Based Hybrid Biomaterial

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1904
Author(s):  
Juan C. Mejía-Giraldo ◽  
Juan C. Scaiano ◽  
Cecilia Gallardo-Cabrera ◽  
Miguel A. Puertas-Mejía
Keyword(s):  
Phenolic Content ◽  
Methanolic Extract ◽  
Polyphenol Content ◽  
Protection Factor ◽  
Dry Extract ◽  
Natural Extract ◽  
Natural Extracts ◽  
Electrostatic Stability ◽  
Better Than

The aim of this study was to develop a new hybrid biomaterial that could photo-stabilize and improve the photoprotective capacity of a Baccharis antioquensis extract. Different combinations of lignin/gelatin/natural extract were applied to prepare hybrid biomaterial nanoparticles (NPs), which were then incorporated into an emulsion. The in vitro photoprotection and photostability were evaluated. The methanolic extract showed high phenolic content (646.4 ± 9.5 mg GAE/g dry extract) and a DPPH radical assay revealed that the antiradical capacity of the extract (0.13 to 0.05 g extract/mmol DPPH) was even better than that of BHT. The particle size of the hybrid biomaterial ranged from 100 to 255 nm; a polydispersity index (PdI) between 0.416 and 0.788 is suitable for topical use in dermocosmetic products. The loading capacity of the extract ranged from 27.0 to 44.5%, and the nanoparticles (NPs) showed electrostatic stability in accordance with the zeta potential value. We found that the formulation based on lignin: extract (1:1 ratio) and gelatin: lignin: extract (0.5:0.5:1 ratio) demonstrated photoprotection qualities with a sun protection factor (SPF) ranging from 9.4 to 22.6. In addition, all the hybrid NP-formulations were time-stable with %SPFeff and %UVAPFeff greater than 80% after exposure to 2 h of radiation. These results suggest that the hybrid biopolymer-natural extract improved the photoprotection and photostability properties, as well as the antiradical capacity, of the B. antioquensis extract, and may be useful for trapping high polyphenol content from natural extracts, with potential application in cosmeceutical formulations.

scholarly journals Wines with Increased Lignan Content by the Addition of Lignan Extracts

2016 ◽  
Vol 34 (No. 5) ◽  
pp. 439-444 ◽  
Author(s):  
Balík Josef ◽  
Híc Pavel ◽  
Kulichová Jana ◽  
Novotná Pavla ◽  
Tříska Jan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Storage Time ◽  
Red Wine ◽  
White Wine ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Ethanol Extracts ◽  
One Year ◽  
And Storage

Red and white wines [Grüner Veltliner white wine and Blue Limberger (Blaufränkisch) red wine (vintage 2013)] were enriched with lignan hydroxymatairesinol originated from spruce knots. These spruce knots with removed resin were extracted with ethyl alcohol of agricultural origin. Ethanol extracts of lignans were then used to enrich wine sorts. Enriched wines were stored for 13 months. At 2, 6, and 13 months, samples were taken and subjected to a variety of analyses and sensory evaluations. Analyses included 7-hydroxymatairesinol and alpha-conidendrin lignan content, antioxidant activity (as determined by FRAP), total polyphenols, and sensory evaluation. The obtained data were evaluated using the analysis of variance to determine which factors e.g. wine type, quantity of added lignan extracts, additional sugar, method of preservation, and storage time had the most significant influence on lignan content, antioxidant activity and polyphenol content. In all cases the lignan content in the wines was significantly influenced by the addition of lignan extracts. After one year of storage, lignan contents changed only moderately and added lignans were stable in stored wines. Total polyphenol content in wines and the antioxidant activity of wines were significantly influenced by the type of wine (i.e., red or white). The presented method of wine enrichment with lignans opens the door for the production of extra quality wines.

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