scholarly journals Photoprotection and Photostability of a New Lignin-Gelatin-Baccharis antioquensis-Based Hybrid Biomaterial

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1904
Author(s):  
Juan C. Mejía-Giraldo ◽  
Juan C. Scaiano ◽  
Cecilia Gallardo-Cabrera ◽  
Miguel A. Puertas-Mejía
Keyword(s):  
Phenolic Content ◽  
Methanolic Extract ◽  
Polyphenol Content ◽  
Protection Factor ◽  
Dry Extract ◽  
Natural Extract ◽  
Natural Extracts ◽  
Electrostatic Stability ◽  
Better Than

The aim of this study was to develop a new hybrid biomaterial that could photo-stabilize and improve the photoprotective capacity of a Baccharis antioquensis extract. Different combinations of lignin/gelatin/natural extract were applied to prepare hybrid biomaterial nanoparticles (NPs), which were then incorporated into an emulsion. The in vitro photoprotection and photostability were evaluated. The methanolic extract showed high phenolic content (646.4 ± 9.5 mg GAE/g dry extract) and a DPPH radical assay revealed that the antiradical capacity of the extract (0.13 to 0.05 g extract/mmol DPPH) was even better than that of BHT. The particle size of the hybrid biomaterial ranged from 100 to 255 nm; a polydispersity index (PdI) between 0.416 and 0.788 is suitable for topical use in dermocosmetic products. The loading capacity of the extract ranged from 27.0 to 44.5%, and the nanoparticles (NPs) showed electrostatic stability in accordance with the zeta potential value. We found that the formulation based on lignin: extract (1:1 ratio) and gelatin: lignin: extract (0.5:0.5:1 ratio) demonstrated photoprotection qualities with a sun protection factor (SPF) ranging from 9.4 to 22.6. In addition, all the hybrid NP-formulations were time-stable with %SPFeff and %UVAPFeff greater than 80% after exposure to 2 h of radiation. These results suggest that the hybrid biopolymer-natural extract improved the photoprotection and photostability properties, as well as the antiradical capacity, of the B. antioquensis extract, and may be useful for trapping high polyphenol content from natural extracts, with potential application in cosmeceutical formulations.

scholarly journals Extract from Broccoli Byproducts to Increase Fresh Filled Pasta Shelf Life

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 621
Author(s):  
Luisa Angiolillo ◽  
Sara Spinelli ◽  
Amalia Conte ◽  
Matteo Alessandro Del Nobile
Keyword(s):  
Shelf Life ◽  
Sensory Quality ◽  
Phenolic Content ◽  
Control Sample ◽  
In Vitro Digestion ◽  
Nutritional Content ◽  
Life Test ◽  
Natural Extract ◽  
Before And After

The aim of the study was to evaluate the efficacy of extract from broccoli byproducts, as a green alternative to chemical preservation strategies for fresh filled pasta. In order to prove its effectiveness, three different percentages (10%, 15%, and 20% v/w) of extract were added to the filling of pasta. A shelf life test was carried out by monitoring microbiological and sensory quality. The content of phenolic compounds before and after in vitro digestion of pasta samples was also recorded. Results underlined that the addition of the natural extract helped to record a final shelf life of about 24 days, that was 18 days longer in respect to the control sample. Furthermore, results highlighted that the addition of byproducts extract to pasta also increased its phenolic content after in vitro digestion. Therefore, broccoli byproducts could be valorized for recording extracts that are able to prolong shelf life and increase the nutritional content of fresh filled pasta.

In- vitroantioxidant and anti-obesity properties of Bauhinia variegata

2017 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
M. M. Patil ◽  
T. Anand ◽  
N. Ilaiyaraja ◽  
Farhath Khanum
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Chloroform Extract ◽  
Polyphenol Content ◽  
Bioactive Properties ◽  
The Family ◽  
Bauhinia Variegata ◽  
And Oxidative Stress

<p><em>Bauhinia variegata</em> L (Synonyms: <em>Phanera variegata</em> Benth), commonly known as orchid tree or Kachnar belongs to the family of Leguminosae and found throughout India. All parts of the plants are used in the treatment of many ailments such as obesity, dyspepsia, bronchitis, leprosy, ulcer <em>etc.</em> The objective of the present study was to assess the i<em>n- vitro</em> antioxidant and anti-obesity properties of different solvent (hexane, chloroform, ethyl acetate, acetone, methanol and distilled water) extracts of <em>Bauhinia variegata</em>leaves. All the solvent extracts were found to contain different phytochemicals with bioactive properties at varying concentrations.The results of extraction showed that methanol was the suitable solvent for extraction of phytochemical as it recorded highest yield of 6.80%. The phytochemical assay results showed the highest polyphenol content in methanol extract (243 μg GAE/mg) and flavonoids content in chloroform extract (1.87 μg CE/g).The <em>in-vitro</em> antioxidant and anti obesity property was observed more in methanolic extract than the other extracts. The overall study suggests that <em>Bauhinia variegata</em>leaf extract could be a potential ingredient for the preparation of nutraceuticals to treat obesity and oxidative stress related complications. </p>

scholarly journals Antioxidant Activity, Polyphenols Content and Antimicrobial Activity of Several Native Pteridophytes of Romania

2012 ◽  
Vol 40 (1) ◽  
pp. 53 ◽  
Author(s):  
Liliana Cristina SOARE ◽  
Mariana FERDES ◽  
Stefan STEFANOV ◽  
Zapriana DENKOVA ◽  
Radosveta NICOLOVA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Fresh Weight ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Brevibacterium Flavum ◽  
Methanolic Extracts ◽  
Cosmetic Ingredients ◽  
Better Than

The aim of this paper was to test the antioxidant activity, polyphenols content and antimicrobial activity of crude extracts obtained from leaves of pteridophyte species commonly found in Romania. The ORAC (Oxygen Radical Absorbance Capacity) of the investigated ferns varied between 421.90 ?mol TE (Trolox equivalents/g FW (fresh weight) in Dryopteris filix-mas and 128.18 ?mol TE/g FW in D. affinis. Methanolic extracts obtained from leaves of ferns have similar antioxidant activity to that of some medicinal plants. Polyphenols content in the leaves of ferns varies between 2340 mg Gallic acid equivalents (GAE)/100 g FW in D. filix-mas and 887 mg GAE/100 g FW in D. affinis. The correlation coefficient between ORAC and the total polyphenol content was R=0.985. This correlation suggests that phenolic compounds are major contributors to the antioxidant activity. The methanolic extract obtained from ferns inhibits the growth of Gram negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa NBJMCC1390, Salmonella abony and Gram positive Staphyllococcus aureus ATCC 25093 and Enterococcus faecalis. The highest antimicrobial activity was determined for the Dryopteris extract. The antimicrobial activity of methanolic extracts obtained from leaves of D. filix-mas and D. affinis is better than the A. filix-femina in the case of Brevibacterium flavum ATCC 14067, Sarcina sp., Bacillus cereus ATCC 1390, Saccharomyces cerevisiae and Aspergillus niger. The tested ferns could be used as cosmetic ingredients, as preservatives in food or in antimicrobial therapy.

scholarly journals Spicy Herb Extracts as a Potential Improver of the Antioxidant Properties and Inhibitor of Enzymatic Browning and Endogenous Microbiota Growth in Stored Mung Bean Sprouts

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 425
Author(s):  
Małgorzata Sikora ◽  
Urszula Złotek ◽  
Monika Kordowska-Wiater ◽  
Michał Świeca
Keyword(s):  
Mung Bean ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Cation Radical ◽  
Total Phenolic ◽  
Natural Extracts ◽  
Mung Bean Sprouts ◽  
Herb Extracts ◽  
Bean Sprouts

The quality and shelf life of sprouts can be improved by postharvest application of water herb extracts. The effect of water infusions of marjoram, oregano, basil, and thyme on the phenolic content, antioxidant potential, and the microbiological and consumer quality of stored mung bean sprouts was studied. Compared to the control, the treatments increased total phenolic content. The highest amounts were determined in sprouts soaked in the thyme extract (6.8 mg/g d.m.). The infusions also inhibited the activity of enzymes utilizing phenolics, and marjoram and oregano were found to be the most effective. The increase in the level of phenolics was reflected in enhanced antioxidant properties (ability to quench cation radical ABTS•+, reducing and chelating power). Both total phenolics and flavonoids, as well as antioxidant capacities, were highly bioaccessible in vitro. All the natural extracts effectively reduced the growth of total mesophilic bacteria, coliforms, and molds (they were more effective than ascorbic and kojic acids). The treatments did not exert a negative influence on the sensory properties or nutritional value of the sprouts, and even improved starch and protein digestibility. These results are very promising and may suggest a wider used of natural extracts as preservatives of minimally processed food.

scholarly journals Polyphenol Composition and (Bio)Activity of Berberis Species and Wild Strawberry from the Argentinean Patagonia

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3331 ◽  
Author(s):  
Melina F. Chamorro ◽  
Gabriela Reiner ◽  
Cristina Theoduloz ◽  
Ana Ladio ◽  
Guillermo Schmeda-Hirschmann ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Diode Array ◽  
Tandem Mass ◽  
Polyphenol Content ◽  
Argentinean Patagonia ◽  
Wild Strawberry ◽  
Array Detection ◽  
Polyphenol Profile ◽  
In Vitro Antioxidant Activity

The Argentinean Patagonia berries Berberis microphylla, Berberis darwinii, and Fragaria chiloensis ssp. chiloensis f. patagonica were investigated for their polyphenol content and composition by means of liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. The in vitro antioxidant activity and inhibition of metabolic syndrome-associated enzymes (α-glucosidase, α-amylase, and lipase) of the fruit extracts was assessed. The most complex polyphenol profile was found in the Berberis samples, with 10 anthocyanins, 27 hydroxycinnamic acids, 3 proanthocyanidins, 2 flavan-3-ol, and 22 flavonols. Fragaria presented four anthocyanins, nine ellagitannins, two proanthocyanidin dimers, one flavan-3-ol, and five flavonols. The Berberis samples showed the best antioxidant capacity, while Fragaria displayed better activity against α-glucosidase and lipase. The phenolic content and composition of the Argentinean Patagonia berries was similar to that reported for Chilean samples but with some chemical differences between Eastern (Argentina) and Western (Chile) Patagonia. The data obtained supports the consumption of these berries as sources of beneficial polyphenols.

scholarly journals Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations

2021 ◽  
Vol 8 ◽  
Author(s):  
Jose Aguilera ◽  
Miguel Vicente-Manzanares ◽  
María Victoria de Gálvez ◽  
Enrique Herrera-Ceballos ◽  
Azahara Rodríguez-Luna ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Biological Effects ◽  
Contact Hypersensitivity ◽  
Immune Protection ◽  
Protection Factor ◽  
Natural Extract ◽  
Precise Knowledge ◽  
Polypodium Leucotomos ◽  
Booster Effect

Background: Novel approaches to photoprotection must go beyond classical MED measurements, as discoveries on the effect of UV radiation on skin paints a more complex and multi-pronged scenario with multitude of skin cell types involved. Of these, photoimmunoprotection emerges as a crucial factor that protects against skin cancer and photoaging. A novel immune parameter is enabled by the precise knowledge of the wavelength and dose of solar radiation that induces photoimmunosupression. Natural substances, that can play different roles in photoprotection as antioxidant, immune regulation, and DNA protection as well as its possible ability as sunscreen are the new goals in cosmetic industry.Objective: To analyze the effect of a specific natural extract from Polypodium leucotomos (PLE, Fernblock®), as part of topical sunscreen formulations to protect from photoimmunosuppression, as well as other deleterious biological effects of UV radiation.Methods: The possible sunscreen effect of PLE was analyzed by including 1% (w/w) PLE in four different galenic formulations containing different combinations of UVB and UVA organic and mineral filters. In vitro sun protection factor (SPF), UVA protection factor (UVA-PF), contact hypersensitivity factor (CHS), and human immunoprotection factor (HIF) were estimated following the same protocol as ISO 24443:2012 for in vitro UVA-PF determination.Results: PLE-containing formulations significantly reduced UV radiation reaching to skin. Combination of UVB and UVA filters with PLE increased SPF and UVAPF significantly. PLE also increased UV immune protection, by elevating the contact hypersensitivity factor and the human immunoprotective factor of the sunscreen formulations.Conclusion: This study confirms the double role of PLE in photoprotection. Together to the biological activity shown in previous works, the UV absorption properties of PLE confers a booster effect when it is supplemented in topical sunscreens increasing the protection not only at level of erythema and permanent pigment darkening but also against two photoimmunoprotection factors.

scholarly journals Reducing Sugar, Total Phenolic Content, and Antioxidant Potential of Nepalese Plants

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Deepa Khatri ◽  
Sumit Bahadur Baruwal Chhetri
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Total Phenolic ◽  
Dry Extract ◽  
Pharmaceutical Industries ◽  
In Vitro Antioxidant Activity

The aim of the present study was to investigate the reducing sugar, total phenolic content, and in vitro antioxidant activity of 70% ( v / v ) ethanolic extract of seven medicinal plants grown in Nepal. The reducing sugar content and total phenolic content were determined by 3,5-dinitrosalicylic acid (DNSA) and the Folin–Ciocalteu method, respectively. The in vitro antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The reducing sugar content of the studied plant samples ranged from 6.89 ± 2.44 to 674.13 ± 2.43  mg GE/g dry extract weight and the total phenolic content ranged from 14.87 ± 0.41 to 281.71 ± 1.47  mg GAE/g dry extract weight. The reducing sugar and total phenolic content were found highest in Ficus glaberrima. Antioxidant activity was found highest in Melastoma malabathricum (IC50 value = 6.27  μg/mL), followed by F. glaberrima (IC50 value = 11.7  μg/mL). A positive and significant correlation was found between (i) total phenolic content and reducing sugar content and (ii) total phenolic content and antioxidant activity. The present study is the first study for the analysis of reducing sugar content of selected plants and for the scientific exploration of F. glaberrima. The present result suggests that the various parts of these studied plants could be assumed as a rich source of biologically active compounds and considered beneficial for the food and pharmaceutical industries.

scholarly journals Impact of Gastrointestinal In Vitro Digestion and Deficit Irrigation on Antioxidant Activity and Phenolic Content Bioaccessibility of “Manzanilla” Table Olives

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Lucía Sánchez-Rodríguez ◽  
Marina Cano-Lamadrid ◽  
Ángel A. Carbonell-Barrachina ◽  
Francisca Hernández ◽  
Esther Sendra
Keyword(s):  
Antioxidant Activity ◽  
Deficit Irrigation ◽  
Phenolic Content ◽  
Soluble Fraction ◽  
In Vitro Digestion ◽  
Antioxidant Potential ◽  
Table Olives ◽  
Polyphenol Content ◽  
Regulated Deficit Irrigation

This was the first study investigating the polyphenol content, antioxidant potential, and polyphenol bioaccessibility after in vitro digestion of table olives grown using regulated deficit irrigation (RDI) treatments to save irrigation water. Two experiments were carried out: (i) experiment A, where RDI was applied during the pit hardening stage and (ii) experiment B, where RDI was applied during the rehydration stage. Only slight differences among irrigation treatments were observed in two antioxidant assays (ABTS+• and DPPH•) and on TPC for the soluble fraction after in vitro digestion. An average of 1 g gallic acid equivalents kg−1 of table olives were found after digestion. Approximately, 12% of the polyphenols of table olives were bioaccessible for human absorption. Saving water techniques influence neither the final polyphenol content and antioxidant potential of table olives nor the bioaccessibility of polyphenols. The consumption of 40 g of table olives will provide 40 mg of bioaccessible polyphenols able to provide associated health benefits (∼7% of the daily polyphenols intake recommendation).

scholarly journals Antioxidant and Hepatoprotective properties of Helianthus annuus seed extract against paracetamol-induced liver toxicity

2020 ◽  
Vol 9 (5) ◽  
pp. 361-366
Author(s):  
Abena Amponsaa Brobbey ◽  
◽  
Yakubu Jibira ◽  
Baba Fuseini ◽  
Richard Nii-Lamptey ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Helianthus Annuus ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Total Phenol Content

This experiment aimed to evaluate the antioxidant and hepatoprotective effect of methanolic extract of Helianthus annuus (sunflower) seeds against paracetamol induced liver injury. Four groups of rats (n = 3) were used and administered orally once daily with H. annus methanolic extract (100, 300, and 500 mg/kg) for 7 days, followed by the induction of hepatotoxicity using acetaminophen. The blood and liver samples were subjected to liver function biochemical and lipid peroxidation assay. The extract was also subjected to in-vitro antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay. The total phenolic content and total flavonoid content were also evaluated. The extract was found to have a total flavonoids of 7.72 ± 2.3 mg/g quercetin equivalence and total phenolic content of 40.60244 ± 1.14 mg/g Gallic acid equivalence. H. annus methanolic extract exhibited a significant decrease (P < 0.0001) in Alanine aminotransferase, alkaline phosphatase, and aspartate aminotransferase enzyme levels. The extract exhibited antioxidant activity and contained high total phenol content. H. annus methanol extract was found to possess a potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content.

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