scholarly journals Antimicrobial Activity, Phenolic and Antioxidant Content of Extracts from Cuajilote (Parmentiera aculeata Kunth) Fruits at Different Degrees of Ripening

2021 ◽  
Vol 65 (2) ◽  
Author(s):  
Cristina Santiago Ruiz ◽  
Viviana Noemi Nuricumbo Lievano ◽  
Mariana Gabriela Chapa Barrios ◽  
Gilber Vela Gutiérrez ◽  
Arturo Velázquez
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Aqueous Ethanol ◽  
Polyphenol Content ◽  
Methanolic Extracts ◽  
Ethanolic Extracts ◽  
Ripe Stage

Abstract. Antioxidants and phenolic compounds are involved in antioxidative processes, decreasing the activity of free radicals. Cuajilote (Parmentiera aculeata) is a crop of the state of Chiapas, Mexico, mainly used as feed for cattle, unknown the compounds that the fruit may contain, but many researches indicate that this fruit has been used for therapeutic purposes. Therefore, the purpose of the present study is the evaluation of the antioxidant, antibacterial and polyphenol content of pulp and seed of cuajilote. The recollected fruit were classified by degree of ripeness (green (G), mature green ripe (MG) and ripe (R)). The samples were dry at 40 °C. Aqueous, ethanol and methanolic extracts were obtained from degree of ripeness mentioned above.  Higher antioxidant activity was obtained from ethanolic extracts of ripe pulp, greater polyphenol content from methanolic extracts from ripe pulp and antibacterial activity in ethanolic extracts from cuajilote pulp. The pulp has a higher antioxidant activity and polyphenol concentration in the ripe stage. Resumen. Los antioxidantes y compuestos fenólicos intervienen en los procesos antioxidantes, disminuyendo la actividad de los radicales libres. El Cuajilote (Parmentiera aculeata) es un cultivo del estado de Chiapas, México, utilizado principalmente como alimento para el ganado, desconociendo los compuestos que el fruto puede contener, pero muchas investigaciones indican que el fruto se ha utilizado para fines terapéuticos. Por lo tanto, el propósito del presente estudio es la evaluación del contenido antioxidante, antibacteriano y de polifenoles de la pulpa y la semilla de cuajilote. Los frutos recogidos se clasificaron por grado de maduración (verde (G), maduro-verde-maduro (MG) y maduro (R)). Las muestras se secaron a 40 °C, los extractos acuosos, etanólico y metanólico contuvieron los compuestos de interés. Se obtuvo actividad antioxidante mayor en los extractos etanólicos de pulpa madura; un mayor contenido de polifenoles a partir de extractos metanólicos de pulpa madura y elevada actividad antibacteriana en extractos etanólicos de la pulpa de cuajilote. La pulpa presenta superior actividad antioxidante y concentración de polifenoles en la etapa madura.

scholarly journals Antioxidant Activity, Polyphenols Content and Antimicrobial Activity of Several Native Pteridophytes of Romania

2012 ◽  
Vol 40 (1) ◽  
pp. 53 ◽  
Author(s):  
Liliana Cristina SOARE ◽  
Mariana FERDES ◽  
Stefan STEFANOV ◽  
Zapriana DENKOVA ◽  
Radosveta NICOLOVA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Fresh Weight ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Brevibacterium Flavum ◽  
Methanolic Extracts ◽  
Cosmetic Ingredients ◽  
Better Than

The aim of this paper was to test the antioxidant activity, polyphenols content and antimicrobial activity of crude extracts obtained from leaves of pteridophyte species commonly found in Romania. The ORAC (Oxygen Radical Absorbance Capacity) of the investigated ferns varied between 421.90 ?mol TE (Trolox equivalents/g FW (fresh weight) in Dryopteris filix-mas and 128.18 ?mol TE/g FW in D. affinis. Methanolic extracts obtained from leaves of ferns have similar antioxidant activity to that of some medicinal plants. Polyphenols content in the leaves of ferns varies between 2340 mg Gallic acid equivalents (GAE)/100 g FW in D. filix-mas and 887 mg GAE/100 g FW in D. affinis. The correlation coefficient between ORAC and the total polyphenol content was R=0.985. This correlation suggests that phenolic compounds are major contributors to the antioxidant activity. The methanolic extract obtained from ferns inhibits the growth of Gram negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa NBJMCC1390, Salmonella abony and Gram positive Staphyllococcus aureus ATCC 25093 and Enterococcus faecalis. The highest antimicrobial activity was determined for the Dryopteris extract. The antimicrobial activity of methanolic extracts obtained from leaves of D. filix-mas and D. affinis is better than the A. filix-femina in the case of Brevibacterium flavum ATCC 14067, Sarcina sp., Bacillus cereus ATCC 1390, Saccharomyces cerevisiae and Aspergillus niger. The tested ferns could be used as cosmetic ingredients, as preservatives in food or in antimicrobial therapy.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

scholarly journals Comparative study of some bioactive compounds and their antioxidant activity of some berry types

10.5219/1132 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 515-523 ◽  
Author(s):  
Amina Aly ◽  
Rabab Maraei ◽  
Omneya Abou El-Leel
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Environmental Conditions ◽  
The Body ◽  
Harvest Time ◽  
Berry Fruits ◽  
Red Currant

Berries are wealthy in bioactive compounds like phenolic compounds and flavonoids that are deemed antioxidants and are great important to health. This research was performed to examine, recognize and compare bioactive compounds in certain types of berries and their antioxidant activity. The data show that blue berry, black berry and Egyptian black mulberry contain the highest content of most bioactive compounds such as phenolic compounds, flavonoids and tannins, while long mulberry and red currant berry have the lowest content for most of these compounds. They therefore, contain the highest value of antioxidant activity. The chemical composition of the berries varies depending on cultivar, variety, location of growth, environmental conditions and harvest time, as well as post-harvest treatments therefore the composition differed from berry fruit to another. Thus, berry fruits are very useful in nutrition to protect the body from many diseases because of its containment of these compounds, which act as free radicals scavenger that harm the body and thus rid the body of many harmful toxins.

Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Bachra Khettal ◽  
Nabil Kadri ◽  
Karim Tighilet ◽  
Ahmed Adjebli ◽  
Farid Dahmoune ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
The Other ◽  
Enzymatic Browning ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Total Phenolic Compounds ◽  
Methanolic Extracts ◽  
Citrus Leaves ◽  
Browning Inhibition

AbstractBackgroundPhenolic compounds fromMethodsMethanolic and aqueous extracts were isolated fromResultsThe results showed that aqueous extracts of all species are rich in total phenolic compounds and flavonoids (from 68.23 to 125.28 mg GAE/g DM) and (from 11.99 to 46.25 mg QE/g DM) respectively. The methanolic and aqueous extracts were examined forConclusionsIn this investigation, the assessment of antiperoxidase activity proved that the leaves extracts of different species were able to inhibit peroxidase activity. However, this inhibition varied with the species and the source of these enzymes. On the other hand, the aqueous extracts of different species showed moderate inhibition of polyphenol oxidase, while no effect on these enzymes was obtained with methanolic extracts.

scholarly journals Study of preparations of bee pollen extracts, antioxidant and antibacterial activity

2007 ◽  
Vol 31 (6) ◽  
pp. 1818-1825 ◽  
Author(s):  
Solange Teresinha Carpes ◽  
Rosicler Begnini ◽  
Severino Matias de Alencar ◽  
Maria Lúcia Masson
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Activity Index ◽  
Ethanolic Extract ◽  
Ethanol Solution ◽  
Pollen Extract ◽  
Extraction Condition ◽  
Polyphenol Compounds ◽  
Significant Difference

The aim of this study was to determine the antioxidant activity, phenolic content and antibacterial activity of pollen extracts obtained with different concentrations of ethanol. Each extraction condition (ethanol solutions from 40 to 90%) had a different effect in the phenolic compounds content. Although, the pollen extract obtained at 60, 70 and 80% of ethanol showed relatively higher levels of phenolic compounds (>10 mg/g) and did not present statistical significant difference between the extraction conditions. The amount of total phenolics ranged from 3.6 to 8.1 and 6.6 to 10.9 mg GAE/g for Alagoas state and Parana state pollen, respectively. The higher value for antioxidant activity index was 83.30% for the pollen from Alagoas state and 81.15 % for Parana state pollen. The highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds. Staphylococcus aureus was inhibited by the ethanolic extract of Alagoas state pollen in all the concentrations of solvent, except the ethanolic extract of pollen at 90%. The extract at 60% of ethanol solution (Parana sample) inhibited Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella sp.

scholarly journals Polyphenol Content and Antioxidant Activity of Stevia and Peppermint as a Result of Organic and Conventional Fertilization

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Lina Garcia-Mier ◽  
Adriana E. Meneses-Reyes ◽  
Sandra N. Jimenez-Garcia ◽  
Adan Mercado Luna ◽  
Juan Fernando García Trejo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Stevia Rebaudiana ◽  
Mentha Piperita ◽  
Total Phenols ◽  
Organic Fertilization ◽  
Polyphenol Content ◽  
Stevia Rebaudiana Bertoni ◽  
Chemical Fertilization ◽  
Therapeutic Properties

Stevia rebaudiana Bertoni and Mentha piperita are plants that generate interest mainly due to the presence of bioactive compounds in their leaves, such as phenolics. Studies indicate that phenolics have pharmacological and therapeutic properties, including antioxidant activity. Phenolic compounds may be affected by the type of fertilization. For this reason, organic and chemical fertilization were evaluated along with antioxidant activity. Results showed significant differences for total phenols in organic peppermint (62% higher content). Also, DPPH test displayed differences for peppermint and stevia (572% and 16% greater in organic). Organic fertilization may be alternative for producing high added agricultural and commercial products.

scholarly journals Antimicrobial Activity Exerted by Total Extracts of Germander

2019 ◽  
Vol 70 (9) ◽  
pp. 3242-3244
Author(s):  
Raul Chioibas ◽  
Razvan Susan ◽  
Monica Susan ◽  
Ovidiu Mederle ◽  
Delia Berceanu Vaduva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Fungal Species ◽  
Polyphenolic Compounds ◽  
Biologically Active ◽  
E Coli ◽  
Methanolic Extracts ◽  
Teucrium Polium ◽  
Active Substances

In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.

scholarly journals In-vitro Antioxidant Activity and Antimicrobial Activity of Hydroalcoholic Extracts of Vernonia cinerea

2019 ◽  
Vol 9 (1) ◽  
pp. 225-228
Author(s):  
Jaswinder Mehta ◽  
Peenu Mahendra Joshi ◽  
Priyanka Kushwaha ◽  
Geeta Parkhe
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Vernonia Cinerea

The aim of present study was to estimate the in vitro antioxidant potential and antimicrobial activity of hydroalcoholic extract of Vernonia cinerea. Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay using ascorbic acid as standard antioxidant. The extract was found to scavenge effectively the free radicals. The total flavonoid contents were determined by established methods and were found to be 0.547 mg/100mg in quercitin equivalents. Antimicrobial activity was performed against 2 stains of human pathogenic bacteria by well diffusion method. Hydroalcoholic extract of Vernonia cinerea showed good antimicrobial activity against gram positive bacteria. The antioxidant activities may be attributed to the presence of significant amounts of flavonoid compounds. Results indicated that hydroalcoholic extract of Vernonia cinerea possess significant antioxidant effect in dose dependent manner, followed by the hydroalcoholic extract of Vernonia cinerea possessed good antimicrobial activity. Keywords: Antioxidant activity, Radical scavenging activity, Free radicals, Antimicrobial activity.

scholarly journals Recovery of Added-Value Compounds from Orange and Spinach Processing Residues: Green Extraction of Phenolic Compounds and Evaluation of Antioxidant Activity

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1800
Author(s):  
María Fernanda Montenegro-Landívar ◽  
Paulina Tapia-Quirós ◽  
Xanel Vecino ◽  
Mònica Reig ◽  
César Valderrama ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Added Value ◽  
Solvent Ratio ◽  
Polyphenol Content ◽  
Ultrasound Assisted Extraction ◽  
Total Polyphenol ◽  
Mechanical Stirring ◽  
Assisted Extraction ◽  
Gallic Acid Equivalent

Phenolic compounds recovery by mechanical stirring extraction (MSE) was studied from orange and spinach wastes using water as a solvent. The statistical analysis showed that the highest total polyphenol content (TPC) yield was obtained using 15 min, 70 °C, 1:100 (w/v) solid/solvent ratio and pH 4 for orange; and 5 min, 50 °C, 1:50 (w/v) solid/solvent ratio and pH 6 for spinach. Under these conditions, the TPC was 1 mg gallic acid equivalent (GAE) g−1 fresh weight (fw) and 0.8 mg GAE g−1 fw for orange and spinach, respectively. MSE substantially increased the phenolic compounds yields (1-fold for orange and 2-fold for spinach) compared with ultrasound-assisted extraction. Furthermore, the antioxidant activity of orange and spinach extracts was evaluated using DPPH, FRAP and ABTS. The obtained results pointed out that the evaluated orange and spinach residues provided extracts with antioxidant activity (2.27 mg TE g−1 and 0.04 mg TE g−1, respectively).

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