scholarly journals High-Intensity Resistance Training Suppresses Exacerbation of Atopic Dermatitis in Mice

2020 ◽  
Vol 29 (4) ◽  
pp. 443-449
Author(s):  
Eun-Ju Choi ◽  
Ki Chan Cho ◽  
Jooyoung Kim
Keyword(s):  
Atopic Dermatitis ◽  
Lymph Node ◽  
Resistance Training ◽  
Mrna Expression ◽  
High Intensity ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Positive Effects ◽  
Draining Lymph Node

PURPOSE: Training generally promotes health and inhibits diseases. However, in patients with atopic dermatitis (AD), training and sweating are significant aggravating factors. This study examined the effect of high-intensity resistance training (HIRT) on AD-like skin lesions in mice.METHODS: Eight-week-old female BALB/c mice were divided into four groups (control, HIRT, AD-only, and AD+HIRT). The mice in the HIRT group performed vertical ladder climbing for four weeks.RESULTS: After four weeks of HIRT, histopathological examination revealed reduced epidermis/dermis and dermal infiltration of inflammatory cells in the mice ear tissue. Additionally, HIRT suppressed serum immunoglobulin (Ig) levels and mRNA expression of pathogenic cytokines in the ear tissue; further, it reduced the size and weight of the draining lymph node (dLN) and non-dLN (ndLN), and the pathogenic cytokine-related mRNA expression of CD4+T cells from dLNs and ndLNs. We thus observed a negative correlation between HIRT and AD symptoms in mice.CONCLUSIONS:The results show that HIRT exerts positive effects in patients with AD.

scholarly journals Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 249 ◽  
Author(s):  
Kim ◽  
Seong ◽  
Choung
Keyword(s):  
Atopic Dermatitis ◽  
Barrier Function ◽  
Molecular Mechanisms ◽  
Inflammatory Diseases ◽  
Skin Barrier ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Morinda Citrifolia ◽  
Skin Barrier Function ◽  
Immune Balance

Morinda citrifolia, a fruit generally known as “Noni”, has been traditionally used in parts of East Asia to relieve inflammatory diseases. Although several studies using noni have been reported, the effect of fermented Morinda citrifolia (F.NONI) on atopic dermatitis (AD) has not been investigated. Thus, we aimed to investigate the improving effect of F.NONI treatment on AD-like skin lesions and elucidate molecular mechanisms. F.NONI was prepared by the fermentation of noni fruit with probiotics and then extracted. F.NONI was orally administrated to NC/Nga mice to evaluate its therapeutic effect on 2,4-dinitrochlorobenzene (DNCB)-induced AD. Oral administration of F.NONI significantly alleviated AD lesions and symptoms such as dermatitis scores, ear thickness, scratching behavior, epidermal thickness, and infiltration of inflammatory cells (e.g., mast cells and eosinophils). In addition, F.NONI treatment reduced the levels of histamine, IgE and IgG1/IgG2a ratio, thymus and activation regulated chemokine (TARC), and thymic stromal lymphopoietin (TSLP) in serum and beneficially modulated the expressions of Th1, Th2, Th17, and Th22-mediated cytokines in lesioned skin and splenocytes. Furthermore, the expressions of the skin barrier-related proteins including filaggrin (FLG), loricrin (LOR), involucrin (IVL), zonula occludens-1 (ZO-1), and occludin (OCC) were restored by F.NONI treatment. Taken together, these results suggest that F.NONI could be a therapeutic agent to attenuate AD-like skin lesions through modulating the immune balance and skin barrier function.

scholarly journals Anti-Inflammatory Effect of Qingpeng Ointment in Atopic Dermatitis-Like Murine Model

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yun-Zhu Li ◽  
Xue-Yan Lu ◽  
Wei Jiang ◽  
Lin-Feng Li
Keyword(s):  
Atopic Dermatitis ◽  
Chinese Medicine ◽  
Mast Cells ◽  
Mrna Expression ◽  
Murine Model ◽  
Skin Lesions ◽  
Serum Immunoglobulin ◽  
Repeated Application ◽  
Immune Dysfunctions ◽  
Inflammatory Effect

Qingpeng ointment (QP) is a Chinese medicine which has been used in treatment of atopic dermatitis (AD) in China. AD-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene (DNFB) on shaved backs. The mice were then treated for 2 weeks with QP of different concentrations and Mometasone Furoate cream (MF), respectively. Macroscopic and microscopic changes of the skin lesions were observed after the treatment. The levels of serum immunoglobulin (Ig) E, tissue interferon (IFN)-γ, and interleukin (IL)-4 and IL-17A and the levels of involucrin, filaggrin, and kallikrein7 in epidermis were measured. The results show severe dermatitis with immune profiles similar to human acute AD. A significant infiltration of CD4+T and mast cells was observed in dermis of lesion but inhibited by QP after a 2-week treatment with it. The production of IgE, IL-4 and the mRNA expression of IL-17A were also suppressed, but the level of IFN-γwas increased. MF suppressed all production of these cytokines and IgE. Accordingly, the mechanism of QP on AD might correlate with its ability of modulating the immune dysfunctions rather than suppressing them. It had no effect on expressions of involucrin and filaggrin, except that its vehicle decreased the level of kallikrein7.

scholarly journals Inhibitory Effect ofNelumbo nucifera(Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Rajendra Karki ◽  
Myung-A Jung ◽  
Keuk-Jun Kim ◽  
Dong-Wook Kim
Keyword(s):  
Atopic Dermatitis ◽  
Severity Score ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
Transepidermal Water Loss ◽  
Inhibitory Effect ◽  
Clinical Severity ◽  
Inflammatory Skin Disease ◽  
Level Measurement ◽  
Different Doses

Atopic dermatitis (AD) is a chronic inflammatory skin disease which has a complex etiology that encompasses immunologic responses. The study was carried out to examine the effect ofNelumbo nucifera(Gaertn.) leaf (NL) on the AD-like skin lesion induced by repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of NC/Nga mice. Three different doses of NL (5, 25, and 50 mg/mice/day) were administered orally from the day of sensitization with DNCB for 4 weeks. The efficacy of NL was judged by histopathological examination, blood IgE level, measurement of transepidermal water loss (TEWL), scratching behavior, and skin severity score. NL resulted in the suppression of clinical severity score, TEWL, scratching behavior, and blood IgE level. Histopathologic analyses revealed that thickening of the epidermis and mast cell degranulation was significantly reduced in NL group. These results suggest that NL may be a useful natural resource for the management of AD.

Distinct strains ofLeishmania majorinduce different cytokine mRNA expression in draining lymph node of BALB/c mice

2012 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
A. Asadpour ◽  
F. Riazi-Rad ◽  
V. Khaze ◽  
S. Ajdary ◽  
M.H. Alimohammadian
Keyword(s):  
Lymph Node ◽  
Mrna Expression ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Draining Lymph Node

scholarly journals Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Soon Re Kim ◽  
Han-Seok Choi ◽  
Hye Sook Seo ◽  
Youn Kyung Choi ◽  
Yong Cheol Shin ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Topical Application ◽  
Immunoglobulin E ◽  
Elevated Serum ◽  
Ethanol Extract ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Serum Ige ◽  
Beneficial Effects ◽  
Blood Eosinophils

KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

scholarly journals Neutrophil elastase in the development of nephrogenic systemic fibrosis (NSF)-like skin lesion in renal failure mouse model

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0259211
Author(s):  
A. Adhipatria P. Kartamihardja ◽  
Syahla Nisaa Amalia ◽  
Akiko Sekiguchi ◽  
Anu Bhattarai ◽  
Ayako Taketomi-Takahashi ◽  
...  
Keyword(s):  
Renal Failure ◽  
Mrna Expression ◽  
Mouse Models ◽  
Neutrophil Elastase ◽  
Inductively Coupled Plasma ◽  
Nephrogenic Systemic Fibrosis ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Control Group ◽  
Inductively Coupled

Although neutrophil elastase (NE) may play a role in lung fibrosis and liver fibrosis, NE involvement in the development of nephrogenic systemic fibrosis has been unclear. We investigated the involvement of NE in the development of nephrogenic systemic fibrosis-like skin lesions post-injections of linear gadolinium-based contrast agents in renal failure mouse models. Renal failure mouse models were randomly divided into three groups: control group (saline), gadodiamide group, and gadopentetate group. Each solution was intravenously administered three times per week for three weeks. The mice were observed daily for skin lesions. Quantification of skin lesions, infiltrating inflammatory cells, and profibrotic cytokines in the affected skin was performed by immunostaining and reverse-transcription polymerase chain reaction (RT-PCR). Blood samples were collected from the facial vein to quantify NE enzymatic activity. The 158Gd concentrations in each sample were quantified using inductively coupled plasma mass spectrometry (ICP-MS). In the gadodiamide group, the mRNA expression of fibrotic markers was increased in the skin lesions compared to the control group. In the gadopentetate group, only collagen 1α and TGF-β mRNA expression were higher than in the control group. The expression of CD3+, CD68+, NE cells and the NE activity in the blood serum were significantly higher in the gadodiamide and gadopentetate groups compared to the control group. Gadolinium concentration in the skin of the gadodiamide group was significantly higher than the gadopentetate group, while almost no traces of gadolinium were found in the control group. Although gadopentetate and gadodiamide affected the fibrotic markers in the skin differently, NE may be involved in the development of fibrosis linked to the GBCAs injections in renal failure mouse models.

Stellera chamaejasme and Its Main Compound Luteolin 7-O-Glucoside Alleviates Skin Lesions in Oxazolone- and 2,4-Dinitrochlorobenzene-Stimulated Murine Models of Atopic Dermatitis

Planta Medica ◽  
2018 ◽  
Vol 85 (07) ◽  
pp. 583-590 ◽  
Author(s):  
Beom-Geun Jo ◽  
No-June Park ◽  
Jonghwan Jegal ◽  
Sangho Choi ◽  
Sang Lee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
Transepidermal Water Loss ◽  
Murine Models ◽  
Hairless Mice ◽  
Main Compound ◽  
Etoh Extract ◽  
Aerial Parts ◽  
Stellera Chamaejasme

Abstract Stellera chamaejasme, also known as “Langdu”, has been traditionally used for the management of skin-related diseases such as psoriasis and skin ulcers. The aim of this study was to determine whether S. chamaejasme and its major component, luteolin 7-O-glucoside, have a preventive effect on the development of atopic dermatitis in oxazolone-treated BALB/c mice and 2,4-dinitrochlorobenzene-treated hairless mice. The epicutaneous applications of oxazolone and 2,4-dinitrochlorobenzene evoke an experimental murine atopic dermatitis-like reaction in BALB/c mouse ears and SKH-1 hairless mice. Atopic skin symptoms, including erythema (redness), pruritus (itching), exudation (weeping), excoriation (peeling), and lichenification (skin thickening), responded to treatment with S. chamaejasme aerial parts EtOH extract for 2 or 3 weeks. Histopathological examination revealed S. chamaejasme aerial parts EtOH extract significantly reduced inflammatory cell infiltration when applied to atopic dermatitis mice. In addition, luteolin 7-O-glucoside, the major active compound of the S. chamaejasme aerial parts EtOH extract, decreased serum IgE and IL-4 levels and transepidermal water loss and increased skin hydration, therefore exhibiting strong anti-atopic dermatitis activity in 2,4-dinitrochlorobenzene-induced atopic dermatitis mice. In this study, we confirmed antipruritic and antidermatitic effects of S. chamaejasme extract and its main component luteolin 7-O-glucoside in atopic dermatitis murine models. The study shows S. chamaejasme aerial parts EtOH extract and luteolin 7-O-glucoside are most likely to be potential drug candidates for atopic dermatitis treatment.

scholarly journals Kampo Medicines for Mite Antigen-Induced Allergic Dermatitis in NC/Nga Mice

2005 ◽  
Vol 2 (2) ◽  
pp. 191-199 ◽  
Author(s):  
Xiu Kun Gao ◽  
Kazutoshi Fuseda ◽  
Tomonori Shibata ◽  
Hiroyuki Tanaka ◽  
Naoki Inagaki ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mrna Expression ◽  
Elevated Serum ◽  
Inflammatory Cells ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Tape Stripping ◽  
Cervical Lymph Nodes ◽  
Serum Ige ◽  
Allergic Dermatitis

We have established an allergic dermatitis model in NC/Nga mice by repeated local exposure of mite antigen for analyzing atopic dermatitis. We examined how four Kampo medicines, Juzen-taiho-to, Hochu-ekki-to, Shofu-san and Oren-gedoku-to, on the dermatitis model to obtain basic information on their usefulness for treating atopic dermatitis. Mite antigen (Dermatophagoides farinaecrude extract) solution at a concentration of 10 mg/ml was painted on the ear of NC/Nga mice after tape stripping. The procedure was repeated five times, at 7 day intervals. An apparent biphasic ear swelling was caused after the fourth and fifth antigen exposures with elevated serum IgE levels and accumulation of inflammatory cells. In the cervical lymph nodes and ear lobes, the five procedures of antigen exposure induced interleukin-4 mRNA expression but reduced interferon-γ mRNA expression. Oral administration of all four Kampo medicines inhibited the formation of ear swelling and inflammatory cell accumulation. Juzen-taiho-to and Hochu-ekki-to apparently prevented the elevation of serum IgE level. Furthermore, the four Kampo medicines showed a tendency to prevent not only the increase in interleukin-4 mRNA expression but also the decrease in interferon-γ mRNA expression. The present results indicate that Juzen-taiho-to, Hochu-ekki-to, Shofu-san and Oren-gedoku-to may correct the Th1/Th2 balance skewed to Th2, and this activity helps inhibit dermatitis in NC/Nga mice. The ability of the Kampo medicines to correct the Th1/Th2 balance seems to underlie their effectiveness in treating of atopic dermatitis.

scholarly journals Gancao Nurish-Yin Decoction Ameliorates Imiquimod-Induced Psoriasis-Like Skin Lesions and Inflammation in Mice

2020 ◽  
Author(s):  
Chen Yong ◽  
Wu Huimei ◽  
Xiao Changhong ◽  
Kutty Selva Nandakumar
Keyword(s):  
Mrna Expression ◽  
Th17 Cells ◽  
Inflammatory Cells ◽  
Severity Index ◽  
Skin Lesions ◽  
Control Group ◽  
Group Model ◽  
Model Group ◽  
Staining Methods ◽  
Relative Mrna Expression

Abstract BackgroundPsoriasis is an autoimmune skin disease with a high clinical prevalence, which is often poses treatment difficulties. In this study, we tested whether Gancao nurish-Yin (GNY) decoction is effective for treating mice with imiquimod (IMQ)-induced psoriasis-like skin lesions. MethodsMice were divided into 4 groups including healthy control group, model group (IMQ-induced psoriasis mice), and two psoriasis induced groups of mice treated with GNY-2.5g/kg and GNY-5g/kg given in the drinking water (n = 8/group). Psoriasis area and severity index (PASI) was used to monitor psoriatic symptoms. H&E staining of dermis and baker’s scores were used to evaluate disease severity. Inflammatory cells positive for Gr-1, CD11c, RORγt and TGF-β were counted by using immunohistochemistry or immunofluorescence staining methods. Flow cytometry was used to analyse CD4+CD17+ Th17 cells, and CD25+Foxp3+ Tregs within splenocyte cell population. Relative mRNA expression of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-ɑ, TGF-β and AMPKɑ1 in the dermis was was semi-quatified using qPCR. ResultsCompared to model group, GNY decoction treated mice, at both the concentrations, improved morphological features, as demonstrated by PASI scores and decreased levels of hyperproliferating keratinocytes. GNY decoction at a higher concentration significantly decreased Gr-1, CD11c, RORγt and TGF-β positive cells in the dermis as well as the differentiated CD4+CD17+ Th17 cells, whereas, CD25+Foxp3+ Tregs were increased in the spleen, mainly with low dose GNY decoction. Furthermore, relative mRNA expression of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-ɑ, TGF-β and AMPKɑ1 within dermis were elevated in mice from IMQ model group, which were significantly recduced in mice treated with GNY decoction at GNY-5g/kg concentration.ConclusionHere we demonstrate disease ameliorating effects of GNY decoction in IMQ-induced psoriasis-like skin lesions in mice, which could form basis for considering GNY decoction for treatment of psoriasis patients.

scholarly journals Effects of 6 Weeks of Traditional Resistance Training or High Intensity Interval Resistance Training on Body Composition, Aerobic Power and Strength in Healthy Young Subjects: A Randomized Parallel Trial

2020 ◽  
Vol 17 (11) ◽  
pp. 4093 ◽  
Author(s):  
Tatiana Moro ◽  
Giuseppe Marcolin ◽  
Antonino Bianco ◽  
Francesco Bolzetta ◽  
Linda Berton ◽  
...  
Keyword(s):  
Body Composition ◽  
Resistance Training ◽  
Muscle Strength ◽  
Lipid Profile ◽  
High Intensity ◽  
Acid Oxidation ◽  
Major Barrier ◽  
Positive Effects ◽  
High Intensity Interval ◽  
Resting Energy

Consistent practice of physical activity has well known positive effects on general health; however, time for exercise remains one major barrier for many. An acute bout of high intensity interval resistance training (HIIRT) increases acute resting energy expenditure (REE) and decreases respiratory ratio (RR), suggesting its potential role on weight loss and increased fatty acid oxidation. The aim of this study was to test the long-term effect of HIIRT on body composition, lipid profile and muscle strength using a randomized parallel trial. Twenty healthy young adults (22.15 ± 1.95 years) were randomized to perform either a HIIRT (N = 11) protocol, consisting of three sets of 6 repetitions at 6 repetition maximum (RM) and then 20 seconds of rest between repetitions until exhaustion repeated for 3 times with 2′30″ rest between sets or a traditional training (TRT, N = 9) protocol of 3 sets of 15 reps with 75 sec of rest between sets. Body composition, resting energy metabolism, aerobic capacity, muscle strength and blood measurements were taken before and after 8 weeks of training. Both protocols enhanced muscle strength, but only HIIRT improved endurance strength performance (+22.07%, p < 0.05) and lean body mass (+2.82%, p < 0.05). REE and RR were unaltered as lipid profile. HIIRT represents a valid training method to improve muscle strength and mass, but its role on body weight control was not confirmed.

Sign in / Sign up

Export Citation Format

Share Document