scholarly journals Monoterpene isolated from the essential oil of Trachyspermum ammi is cytotoxic to multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus strains

2016 ◽  
Vol 49 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Faride Hosseinkhani ◽  
Fereshteh Jabalameli ◽  
Maryam Banar ◽  
Nafiseh Abdellahi ◽  
Morovat Taherikalani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Essential Oil ◽  
Multidrug Resistant ◽  
Trachyspermum Ammi ◽  
And Staphylococcus Aureus

scholarly journals GC-MS analysis, antibacterial, antioxidant study and brine shrimp lethality analysis of Trachyspermum ammi (L.) Sprague

2020 ◽  
Vol 1 (1) ◽  
pp. 45-50
Author(s):  
Shova D.C. ◽  
Binita Maharjan ◽  
Timila Shrestha ◽  
Samjhana Bharati ◽  
Ram Lal Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Brine Shrimp ◽  
Bacterial Species ◽  
Zone Of Inhibition ◽  
Lc50 Value ◽  
Trachyspermum Ammi ◽  
Ic50 Value ◽  
And Staphylococcus Aureus ◽  
Fruit Part

Fruit part of Trachyspermum ammi (L.) Sprague was subjected to extraction of essential oil by hydrodistillation in Clevenger apparatus. So collected essential oil was analyzed by GC-MS for its composition and exhibited the presence of 10 different compounds. The most abundant were γ-terpinene (53.81%) and thymol (29.40%). Antibacterial activity was performed against six bacterial species and Staphylococcus aureus, Enterobacter cloaceae and Bacillus subtilis were the most susceptible to the essential oil showing zone of inhibition (ZOI) 1.4, 1.5 and 1.4 cm respectively. The IC50 value of the oil against DPPH was found to be 0.94 mg/mL. The LC50 value of essential oil of T. ammi against brine shrimp was found 26.2 μg/mL.

scholarly journals Antibacterial activity of lavender essential oil and linalool combined with gentamicin on selected bacterial strains

2019 ◽  
Vol 75 (05) ◽  
pp. 6279-2019
Author(s):  
MICHALINA ADASZYŃSKA-SKWIRZYŃSKA ◽  
DANUTA SZCZERBIŃSKA ◽  
SŁAWOMIR ZYCH
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Synergistic Interaction ◽  
Antibacterial Drug ◽  
Bacterial Strains ◽  
Staphyloccocus Aureus ◽  
And Staphylococcus Aureus

The aim of this study was to verify the existence of the synergistic antibacterial effect of lavender essential oil and linalool individually combined with the antibacterial drug gentamicin. We investigated in vitro the effectiveness of the combinations of gentamicin and lavender essential oil and of gentamicin and linalool against the following strains: Staphylococus aureus ATCC 25923, Staphylococus aureus MRSA and Pseudomonas aeruginosa ATCC 9027. In order to determine the sensitivity of these microorganisms, we determined the minimum inhibitory concentration (MIC – Minimal Inhibitory Concentration). The study of the interaction of gentamicin with lavender oil and linalool was evaluated by the checkerboard method. Synergistic interaction between lavender essential oil and gentamicin and between linalool and gentamicin was observed against Staphylococcus aureus ATCC 25923 and Staphylococcus aureus MRSA. In particular, a very strong synergistic interaction was observed against Staphyloccocus aureus MRSA (lavender essential oil FIC index = 0.14; linalool FIC index = 0.13). In contrast, combinations of gentamicin and lavender essential oil and of gentamicin and linalool were less effective against Pseudomonas aeruginosa growth.

scholarly journals Molecular identification and screening of mushrooms for antibacterial property against Pseudomonas aeruginosa and Staphylococcus aureus

2018 ◽  
Vol 10 (2) ◽  
pp. 791-796 ◽  
Author(s):  
M. Mariselvi ◽  
Earanna Ninganna
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Property ◽  
Schizophyllum Commune ◽  
Its Region ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
And Staphylococcus Aureus

In this study, 18 mushrooms were collected from Gandhi Krishi Vigyana Kendra (GKVK) campus, University of Agricultural Sciences, Bangalore (India) and identified by Internal Transcribed Spacer (ITS) region using National Centre for Biotechnology Information (NCBI) data base and screened for antibacterial property against two skin infection causing bacteria viz.,Pseudomonas aeruginosa and Staphylococcus aureus in dogs. Eleven mushrooms extract inhibited both the bacteria showing their antimicrobials as broad spectrum while inhibiting gram positive as well as gram negative bacteria.  The highest zone of inhibition was recorded from the extract of Lyophyllum sp. The extract of two mushrooms viz., Phlebopus portentosus and Termitomyces sp. inhibited only P. aeruginosa, a gram negative bacterium  and the extract of three mushrooms viz., Agaricus sp, Coprinellus disseminates and Agaricus blazei inhibited only S. aureus, a gram positive bacterium, suggesting spectral specificity of their antimicrobial compounds.  However,  the extracts of two mushrooms viz.,Macrocybe gigantea and Schizophyllum commune did not show inhibition of any of the pathogens.  Hence, this study suggests that the occurrence of potential pharmaceuticals in mushrooms can be exploited for control of multidrug resistant bacteria.

scholarly journals Pyranoanthocyanins Interfering with the Quorum Sensing of Pseudomonas aeruginosa and Staphylococcus aureus

2021 ◽  
Vol 22 (16) ◽  
pp. 8559
Author(s):  
Patrícia Coelho ◽  
Joana Oliveira ◽  
Iva Fernandes ◽  
Paula Araújo ◽  
Ana Rita Pereira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Quantitative Polymerase Chain Reaction ◽  
Cell Communication ◽  
Multidrug Resistant ◽  
Pure Compound ◽  
Bacterial Quorum ◽  
And Staphylococcus Aureus

Bacterial quorum sensing (QS) is a cell-cell communication system that regulates several bacterial mechanisms, including the production of virulence factors and biofilm formation. Thus, targeting microbial QS is seen as a plausible alternative strategy to antibiotics, with potentiality to combat multidrug-resistant pathogens. Many phytochemicals with QS interference activity are currently being explored. Herein, an extract and a compound of bioinspired origin were tested for their ability to inhibit biofilm formation and interfere with the expression of QS-related genes in Pseudomonas aeruginosa and Staphylococcus aureus. The extract, a carboxypyranoanthocyanins red wine extract (carboxypyrano-ant extract), and the pure compound, carboxypyranocyanidin-3-O-glucoside (carboxypyCy-3-glc), did not cause a visible effect on the biofilm formation of the P. aeruginosa biofilms; however, both significantly affected the formation of biofilms by the S. aureus strains, as attested by the crystal violet assay and fluorescence microscopy. Both the extract and the pure compound significantly interfered with the expression of several QS-related genes in the P. aeruginosa and S. aureus biofilms, as per reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results. Indeed, it was possible to conclude that these molecules interfere with QS at distinct stages and in a strain-specific manner. An extract with anti-QS properties could be advantageous because it is easily obtained and could have broad, antimicrobial therapeutic applications if included in topical formulations.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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