scholarly journals Green Synthesis, Characterization and Antimicrobial Evaluation of Silver Nanoparticles for an Intracanal Dressing

2020 ◽  
Vol 31 (5) ◽  
pp. 485-492
Author(s):  
João Felipe Bonatto Bruniera ◽  
Leonado Gabriel-Silva ◽  
Rafael Silva Goulart ◽  
Yara Teresinha Corrêa Silva-Sousa ◽  
Marilisa Guimarães Lara ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Clinical Laboratory ◽  
Antimicrobial Properties ◽  
Absorption Spectrometry ◽  
Minimal Bactericidal Concentration ◽  
Bacterial Strains ◽  
Molecular Absorption Spectrometry ◽  
Antimicrobial Evaluation

Abstract Green chemistry has been applied in different areas due to the growing demands for renewable processes and one of them is nanotechnology. The aim of this study was to characterize a formulation containing silver nanoparticles (AgNPs) produced by a green synthesis and to evaluate its antimicrobial activity. The formulation will be used as an intracanal dressing exploiting the AgNPs’ antimicrobial properties, which are crucial to prevent infections and bacterial reinfections that can compromise endodontic treatments. In the green synthesis, silver nitrate was employed as the precursor salt, maltose as a reducing agent, and gelatin as a stabilizing agent. The formulation was prepared mixing 50 % of a liquid containing the AgNPs and 50 % of hydroxyethylcellulose gel at 1.5 % with proper evaluation of the process inherent parameters. Techniques such as molecular absorption spectrometry and dynamic light scattering were used in characterization step. The antimicrobial activity of the AgNPs against Escherichia coli ATCC 25922, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Streptococcus mutans ATCC 25175 was verified according to National Comittee for Clinical Laboratory Standards (NCCLS) by determining minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The obtained results indicated the formulation containing AgNPs produced by a green synthesis was properly characterized by the selected techniques. Furthermore, the formulation assessment proved that it is suitable for the proposal as well as it has potential to be used as an intracanal dressing since presented antimicrobial activity against all bacterial strains evaluated.

scholarly journals ANTIMICROBIAL EVALUATION OF ETHANOLIC EXTRACT OF SELECTED SEED SHELLS

2020 ◽  
pp. 74-76
Author(s):  
MOUSHUMI BAIDYA ◽  
ANBU J. ◽  
SEMIMUL AKHTAR ◽  
SIPRA SARKAR ◽  
SUDIP KUMAR MANDAL
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Pathogens ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Local Market ◽  
Bacterial Strains ◽  
Study Results ◽  
Fungal Organisms ◽  
Antimicrobial Evaluation

Objective: The study was undertaken to evaluate the antimicrobial activity of ethanolic extract of polyherbal seed shells. Methods: The seed of Momordica charantia, Manikara zapota, Emblica officinalis, Syzygium cumini, collected from the local market, Mathikere, Bangalore, India. Ethanolic extract was prepared from the dried seed powders using solvent 80% ethanol. Initially, antimicrobial activity of the extract was performed by agar well diffusion method against two bacterial strains (Escherichia coli, and Staphylococcus aureus) and two fungal pathogens (Aspergillus niger and Candida albicans). Results: The antimicrobial study results revealed that the test extract was strongly inhibited the growth of bacteria, whereas it was not inhibited the growth of fungal organisms used in this study. Conclusion: The results suggest that ethanolic extract of seeds possess antimicrobial properties which can be used for the treatment of infectious diseases.

Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles using Uraria picta Leaves Extract

2021 ◽  
Vol 13 ◽  
Author(s):  
Satyendra Mishra ◽  
Sanjay Kumavat
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Cost Effective ◽  
Salmonella Typhi ◽  
Single Step ◽  
Average Particle ◽  
Antifungal Action ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi

Background: The rapid synthesis and antimicrobial activity of silver nanoparticles (AgNPs) synthesized using Uraria picta extract were investigated in this paper, and AgNPs were examined for antimicrobial activity against a variety of pathogenic organisms, including bacteria and fungi. Objective: Green synthesis of AgNPs from Uraria picta leaves extract for antimicrobial evaluation against bacteria and fungi using MIC studies. Methods: The AgNPs were created by treating an aqueous extract of Uraria picta leaves with silver nitrate (1 mM) solution and then synthesizing nanoparticles for various studies. Results: The Uraria picta leaves extract can be used to make green synthesis AgNPs effectively. The absorption band at 425 nm in the UV-Vis spectrum confirmed the synthesis of AgNPs. Silver nanoparticles show antimicrobial and antifungal action, according to MIC tests. This work gives a better understanding of how new antimicrobial and antifungal activity develops. Conclusion: AgNPs was synthesized from Uraria picta using a green, cost-effective, rapid, single-step, and simple process that, for the first time in this plant nanoparticles synthesis, showed antimicrobial activity. AgNPs were found to be spherical and oval, with average particle sizes ranging from 12.54 to 25.58 nm. The strong zone of inhibition of AgNPs against Salmonella Typhi, Escherichia coli, Bacillus subtilis, and Staphylococcus aureus demonstrated their antimicrobial activity.

scholarly journals Green Synthesis, Characterization and Antimicrobial activity of Silver Nanoparticles from the Extract of Lagerstroemia speciosa

2021 ◽  
Vol 37 (3) ◽  
pp. 648-655
Author(s):  
Vinit Prakash ◽  
Harpreet Kaur ◽  
Anjana Kumari ◽  
Manoj Kumar ◽  
Sumeet Gupta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Kidney Diseases ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Ag Nps

Lagerstroemia speciosais commonly known as Banaba or Jarul which is used to get rid of various ailments such as fever, urinary infection, decongestion, diarrhoea, mouth ulcers, astringent, diabetes mellitus, kidney diseases, abdominal pains etc. The present work, describes the green synthesis of silver nanoparticles from ethanolic extract of fruits of L.speciosa (Ls-Ag NPs) and their analysis for antimicrobial activities. The characterisation of so obtained nanoparticles have been carried out with help of Field emission scanning electron microscopy (FESEM) and High-resolution transmission electron microscopy (HRTEM). Further, antimicrobial activities of ethanolic extract of fruits of L.speciosa(Ee-Ls), silver oxide (Ag2O), and Ls-Ag NPs have been examined by using well-diffusion method against two bacterial strains: Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) and one fungal strain: Aspergillus niger.It has been observed that the biosynthesized Ls-Ag NPs possess much effective antimicrobial activity against selected strains as compared to Ee-Ls and Ag2O.

scholarly journals Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Ethanolic Extract ◽  
Spherical Shape ◽  
Bacterial Strains ◽  
X Ray ◽  
Coccinia Indica ◽  
Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

scholarly journals Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

RSC Advances ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 2673-2702 ◽  
Author(s):  
Anupam Roy ◽  
Onur Bulut ◽  
Sudip Some ◽  
Amit Kumar Mandal ◽  
M. Deniz Yilmaz
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Mode Of Action ◽  
Antimicrobial Agents ◽  
Recent Advances

In this review, we discuss the recent advances in green synthesis of silver nanoparticles, their application as antimicrobial agents and mechanism of antimicrobial mode of action.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

Green Synthesis and Antimicrobial Activity of Monodispersed Silver Nanoparticles Synthesized Using Lemon Extract

2015 ◽  
Vol 46 (2) ◽  
pp. 291-294 ◽  
Author(s):  
P. Mosae Selvakumar ◽  
Churchil Angel Antonyraj ◽  
Revington Babu ◽  
Arun Dakhsinamurthy ◽  
N. Manikandan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis

scholarly journals Anticancer and Antimicrobial Activity Evaluation of Cowpea-Porous-Starch-Formulated Silver Nanoparticles

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shiara Ramdath ◽  
John Mellem ◽  
Londiwe Simphiwe Mbatha
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Cell Viability ◽  
High Dose ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Anticancer Drug Delivery ◽  
Ic50 Values ◽  
Biosynthesized Agnps

Health issues involving inadequate treatment of diseases such as cancer and microbial infections continue to be the subject of much ongoing recent research. Biosynthesized silver nanoparticles (AgNPs) were characterized using Transmission Electron Microscopy (TEM), Zeta Sizer, Ultraviolet (UV), and Fourier Transform Infrared (FTIR) spectroscopy. Their antimicrobial activity was evaluated on selected Gram-positive and Gram-negative bacterial strains, using the disc diffusion and broth dilution assays. Cell viability profiles were evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis studies on selected human noncancer and cancer cells. The biosynthesized AgNPs were evaluated to be spherical clusters, with sizes between 40 and 70 nm. The absorption peak at 423 nm and the presence of polyphenols confirmed the synthesis and stabilization of these tested AgNPs. The AgNPs showed a good stability of −23.9 ± 1.02 mV. Good antimicrobial activity (6.0–18.0 mm) was seen on all tested bacteria at a minimum inhibitory concentration (MIC) ranging from 5 to 16 μg/ml, with the highest activity seen against Gram-negative Escherichia coli (18 ± 0.5 mm), and the lowest activity was seen against Gram-positive Listeria monocytogenes (6.0 ± 0.4 mm) after treatment with the AgNPs. These NPs showed a concentration-dependent and cell-specific cytotoxicity with low IC50 values (41.7, 56.3, and 63.8 μg/ml). The NPs were well tolerated by tested cells as indicated by a more than 50% cell viability at the high dose tested and low apoptotic indices (<0.2). These findings indicated that these biosynthesized AgNPs showed great potential as effective antibacterial agents and anticancer drug delivery modalities.

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