Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles using Uraria picta Leaves Extract

2021 ◽  
Vol 13 ◽  
Author(s):  
Satyendra Mishra ◽  
Sanjay Kumavat
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Cost Effective ◽  
Salmonella Typhi ◽  
Single Step ◽  
Average Particle ◽  
Antifungal Action ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi

Background: The rapid synthesis and antimicrobial activity of silver nanoparticles (AgNPs) synthesized using Uraria picta extract were investigated in this paper, and AgNPs were examined for antimicrobial activity against a variety of pathogenic organisms, including bacteria and fungi. Objective: Green synthesis of AgNPs from Uraria picta leaves extract for antimicrobial evaluation against bacteria and fungi using MIC studies. Methods: The AgNPs were created by treating an aqueous extract of Uraria picta leaves with silver nitrate (1 mM) solution and then synthesizing nanoparticles for various studies. Results: The Uraria picta leaves extract can be used to make green synthesis AgNPs effectively. The absorption band at 425 nm in the UV-Vis spectrum confirmed the synthesis of AgNPs. Silver nanoparticles show antimicrobial and antifungal action, according to MIC tests. This work gives a better understanding of how new antimicrobial and antifungal activity develops. Conclusion: AgNPs was synthesized from Uraria picta using a green, cost-effective, rapid, single-step, and simple process that, for the first time in this plant nanoparticles synthesis, showed antimicrobial activity. AgNPs were found to be spherical and oval, with average particle sizes ranging from 12.54 to 25.58 nm. The strong zone of inhibition of AgNPs against Salmonella Typhi, Escherichia coli, Bacillus subtilis, and Staphylococcus aureus demonstrated their antimicrobial activity.

Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Dudoa (Hydnocarpus alcalae C.DC.) Leaf Extract As a Reducing and Stabilizing Agent

2019 ◽  
Vol 4 (2) ◽  
pp. 112-124
Author(s):  
Edward K.B. Bragais ◽  
Lynne M. Labaclado
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Cost Effective ◽  
Endemic Plant ◽  
Stabilizing Agent ◽  
One Step ◽  
Ft Ir ◽  
Size And Morphology

Background and Objective: In this study, dudoa (Hydnocarpus alcalae C.DC.) leaf extract was used as a reducing and stabilizing agent in a novel one-step green synthesis of silver nanoparticles. Dudoa is an endemic plant in the province of Legazpi, Philippines and its seed oil was used as an anti-leprotic drug. Method: Therefore, the dudoa leaf extract was used to synthesize silver nanoparticles. Moreover, optimization of various parameters greatly affected the size and morphology of the synthesized AgNPs as indicated by the Ultraviolet-visible (UV-vis) spectrophotometry. The synthesized AgNPs were further characterized using spectral analyses such as XRD, EDX, SEM, FT-IR, TGA and DLS. The antimicrobial activity of synthesized AgNPs was also demonstrated. Results and Discussions: The synthesized AgNPs exhibited a diffraction pattern and a particle size ranging from 22-48 nm. The AgNPs also showed complete inhibitory and mild reactivity against representative pathogenic gram-positive (S.aureus) and gram-negative (E.coli) bacteria. Conclusion: Silver nanoparticles were successfully synthesized using H. alcalae leaf extract. Furthermore, this green synthesis approach appeared to be cost-effective, non-toxic, and eco-friendly which is a best alternative to the conventional chemical methods.

scholarly journals Green Synthesis, Characterization and Antimicrobial Evaluation of Silver Nanoparticles for an Intracanal Dressing

2020 ◽  
Vol 31 (5) ◽  
pp. 485-492
Author(s):  
João Felipe Bonatto Bruniera ◽  
Leonado Gabriel-Silva ◽  
Rafael Silva Goulart ◽  
Yara Teresinha Corrêa Silva-Sousa ◽  
Marilisa Guimarães Lara ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Clinical Laboratory ◽  
Antimicrobial Properties ◽  
Absorption Spectrometry ◽  
Minimal Bactericidal Concentration ◽  
Bacterial Strains ◽  
Molecular Absorption Spectrometry ◽  
Antimicrobial Evaluation

Abstract Green chemistry has been applied in different areas due to the growing demands for renewable processes and one of them is nanotechnology. The aim of this study was to characterize a formulation containing silver nanoparticles (AgNPs) produced by a green synthesis and to evaluate its antimicrobial activity. The formulation will be used as an intracanal dressing exploiting the AgNPs’ antimicrobial properties, which are crucial to prevent infections and bacterial reinfections that can compromise endodontic treatments. In the green synthesis, silver nitrate was employed as the precursor salt, maltose as a reducing agent, and gelatin as a stabilizing agent. The formulation was prepared mixing 50 % of a liquid containing the AgNPs and 50 % of hydroxyethylcellulose gel at 1.5 % with proper evaluation of the process inherent parameters. Techniques such as molecular absorption spectrometry and dynamic light scattering were used in characterization step. The antimicrobial activity of the AgNPs against Escherichia coli ATCC 25922, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Streptococcus mutans ATCC 25175 was verified according to National Comittee for Clinical Laboratory Standards (NCCLS) by determining minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The obtained results indicated the formulation containing AgNPs produced by a green synthesis was properly characterized by the selected techniques. Furthermore, the formulation assessment proved that it is suitable for the proposal as well as it has potential to be used as an intracanal dressing since presented antimicrobial activity against all bacterial strains evaluated.

scholarly journals Preparation of silver nanoparticle by green synthesis and application to leather in enhancements of antimicrobial activity of leather

2021 ◽  
Vol 2098 (1) ◽  
pp. 012037
Author(s):  
E Rohaeti ◽  
A Putri ◽  
K S Budiasih ◽  
N A Ariyanti ◽  
A S N Annisa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Staphylococcus Epidermidis ◽  
Average Particle Size ◽  
Ex Situ ◽  
Diffusion Method ◽  
Average Particle

Abstract The objectives of this study were to prepare nanoparticle by using extract of Tectona grandis L. leaf and to deposit nanoparticle on goat leather via ex situ method, and also to characterize the antimicrobial activity of modified goat leather. The stages in this study were preparing of silver nanoparticles via green synthesis method by using extract of teak leaf, characterizing of nanoparticle by using a UV-Vis spectrophotometer and Particle Size Analyzer (PSA), impregnating the nanoparticle on the pickle leather by ex situ method, and analysing the antibacterial and antifungal activities of modified leather by the diffusion method. The silver nanoparticles were greenish brown in colour, having absorption at the wavelengths of 449 nm which indicator the formation of silver nanoparticles. The silver nanoparticles have an average particle size of 109.9 nm. The results of antimicrobial tests against the bacteria Staphylococcus epidermidis DNCC 6018, Escherichia coli FNCC 0047, and the fungus Candida albicans ANCC 0048 showed that all variations of the leather have different abilities in inhibiting the growth of the bacteria and the fungi. The pickle leathers after modification with nanoparticle Ag showed the highest antibacterial activities against Staphylococcus epidermidis DNCC 6018 and Escherichia coli FNCC 0047.

scholarly journals Single-Step Green Synthesis of Highly Concentrated and Stable Colloidal Dispersion of Core-Shell Silver Nanoparticles and Their Antimicrobial and Ultra-High Catalytic Properties

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1007
Author(s):  
Azam Ali ◽  
Mariyam Sattar ◽  
Fiaz Hussain ◽  
Muhammad Humble Khalid Tareen ◽  
Jiri Militky ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Tannic Acid ◽  
Rate Constant ◽  
Catalytic Properties ◽  
Colloidal Dispersion ◽  
Alkaline Condition ◽  
Core Shell ◽  
Average Particle ◽  
Ag Nps

The versatile one-pot green synthesis of a highly concentrated and stable colloidal dispersion of silver nanoparticles (Ag NPs) was carried out using the self-assembled tannic acid without using any other hazardous chemicals. Tannic acid (Plant-based polyphenol) was used as a reducing and stabilizing agent for silver nitrate in a mild alkaline condition. The synthesized Ag NPs were characterized for their concentration, capping, size distribution, and shape. The experimental results confirmed the successful synthesis of nearly spherical and highly concentrated (2281 ppm) Ag NPs, capped with poly-tannic acid (Ag NPs-PTA). The average particle size of Ag NPs-PTA was found to be 9.90 ± 1.60 nm. The colloidal dispersion of synthesized nanoparticles was observed to be stable for more than 15 months in the ambient environment (25 °C, 65% relative humidity). The synthesized AgNPs-PTA showed an effective antimicrobial activity against Staphylococcus Aureus (ZOI 3.0 mM) and Escherichia coli (ZOI 3.5 mM). Ag NPs-PTA also exhibited enhanced catalytic properties. It reduces 4-nitrophenol into 4-aminophenol in the presence of NaBH4 with a normalized rate constant (Knor = K/m) of 615.04 mL·s−1·mg−1. For comparison, bare Ag NPs show catalytic activity with a normalized rate constant of 139.78 mL·s−1·mg−1. Furthermore, AgNPs-PTA were stable for more than 15 months under ambient conditions. The ultra-high catalytic and good antimicrobial properties can be attributed to the fine size and good aqueous stability of Ag NPs-PTA. The unique core-shell structure and ease of synthesis render the synthesized nanoparticles superior to others, with potential for large-scale applications, especially in the field of catalysis and medical.

scholarly journals Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

RSC Advances ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 2673-2702 ◽  
Author(s):  
Anupam Roy ◽  
Onur Bulut ◽  
Sudip Some ◽  
Amit Kumar Mandal ◽  
M. Deniz Yilmaz
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Mode Of Action ◽  
Antimicrobial Agents ◽  
Recent Advances

In this review, we discuss the recent advances in green synthesis of silver nanoparticles, their application as antimicrobial agents and mechanism of antimicrobial mode of action.

Green Synthesis and Antimicrobial Activity of Monodispersed Silver Nanoparticles Synthesized Using Lemon Extract

2015 ◽  
Vol 46 (2) ◽  
pp. 291-294 ◽  
Author(s):  
P. Mosae Selvakumar ◽  
Churchil Angel Antonyraj ◽  
Revington Babu ◽  
Arun Dakhsinamurthy ◽  
N. Manikandan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis

scholarly journals Synthesis, Molecular Docking and Antimicrobial Evaluation of Some Benzothiazoles

2021 ◽  
Author(s):  
Smita J. Pawar ◽  
Amol Kale ◽  
Priya Zori ◽  
Rahul Dorugade
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Property ◽  
Docking Study ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Good Antibacterial Activity ◽  
Comparable Activity ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi

Abstract Abstract The new series of 2-(substituted amino)-N-(6- substituted-1,3-benzothiazol-2yl) acetamide BTC(a-t) has been synthesized by appropriate synthetic route from substituted 2-amino benzothiazole. The synthesized compounds were screened experimentally for its antimicrobial property against gram positive, gram negative bacteria and fungi. Zone of inhibition and minimum inhibitory concentration of compounds was determined against selected bacterial and fungal strains. Compound BTC-j N-(6-methoxy-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide and compound BTC-r N-(6-nitro-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide found to have good antimicrobial potential. The compound BTC-j has shown good antibacterial activity against S. aureus at MIC of 12.5 µg/ml, B. subtilis at MIC of 6.25µg/ml, E. coli at MIC of 3.125µg/ml and P. aeruginosa at MIC of 6.25µg/ml. No statistical difference in antimicrobial activity of standard and test compounds was found indicating test compounds have comparable activity. Further docking study was carried out to check the probable interactions with the selected protein using V-life MDS 3.5 software. (DNA gyrase, PDB: 3G75). The dock score of compounds and antimicrobial activity found to be consistent.

Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A Review

2021 ◽  
Vol 09 ◽  
Author(s):  
Sarvat Zafar ◽  
Aiman Zafar ◽  
Fakhra Jabeen ◽  
Miad Ali Siddiq
Keyword(s):  
Silver Nanoparticles ◽  
Large Scale ◽  
Scale Up ◽  
Cost Effective ◽  
Biological Properties ◽  
Single Step ◽  
Biological Synthesis ◽  
Nanosized Particles ◽  
Environment Friendly ◽  
Physical And Chemical

: Nanotechnology studies the various phenomena of physio-chemical procedures and biological properties for the generation of nanosized particles, and their rising challenges in the various sectors, like medicine, engineering, agriculture, electronic, and environmental studies. The nanosized particles exhibit good anti-microbial, anti-inflammatory, cytotoxic, drug delivery, anti-parasitic, anti-coagulant and catalytic properties because of their unique dimensions with large surface area, chemical stability and higher binding density for the accumulation of various bio-constituents on their surfaces. Biological approaches for the synthesis of silver nanoparticles (AgNPs) have been reviewed because it is an easy and single-step protocol and a viable substitute for the synthetic chemical-based procedures. Physical and chemical approaches for the production of AgNPs are also mentioned herein. Biological synthesis has drawn attention because it is cost-effective, faster, non-pathogenic, environment-friendly, easy to scale-up for large-scale synthesis, and having no demand for usage of high pressure, energy, temperature, or noxious chemical ingredients, and safe for human therapeutic use. Therefore, the collaboration of nanomaterials with bio-green approaches could extend the utilization of biological and cytological properties compatible with AgNPs. In this perspective, there is an immediate need to develop ecofriendly and biocompatible techniques, which strengthen efficacy against microbes and minimize toxicity for human cells. The present study introduces the biological synthesis of silver nanoparticles, and their potential biomedical applications have also been reviewed.

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